IndraLab

Statements

USP15 is phosphorylated. 22 / 22
| 19 3
sparser
"However, as it is shown here, inhibition of USP15 phosphorylation by curcumin in HeLa cell lysates caused the formation of high-molecular-weight USP15 species, most likely USP15-Ub conjugates ( Figure[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16005295
sparser
"In that study, phosphorylation of USP15 at Thr149 or Thr219 affected USP15 interaction with its substrates; however, they did not affect intrinsic USP15 catalytic activity ( xref )."
32101751
sparser
"Here, we show that USP15 is phosphorylated, and its localization and activity are dependent on the phosphorylation status."
31330151
sparser
"As pervasive mitotic phosphorylation often inactivates protein functions [ xref ], USP15 phosphorylation may abrogate its role in stabilising REST at mitosis, allowing REST to degrade."
29429988
sparser
"In BRCA, several databases, including GEPIA and UALCAN, describe the upregulation of total protein levels and USP15 phosphorylation."
36213818
sparser
"We also found that MDC1-FHA domain bound phosphorylated USP15, and Ser678 phosphorylation was essential for this binding (Fig.  xref )."
30874560
sparser
"Future structural and biochemical studies will help to inform the precise mechanisms that coordinate the phosphorylation of USP15, the binding of TIFAB, and the conformation of USP15."
32101751
sparser
"The phosphorylated USP15 was recruited to DSBs (Fig.  xref and Supplementary Fig.  xref ), while the S678A mutant, which abolished its phosphorylation by ATM, could not be recruited to DSBs (Fig.  xref )."
30874560
sparser
"We next wanted to figure out whether USP15 phosphorylation affects its localization in cells."
30874560
sparser
"Finally, USP15 is phosphorylated by ATR/ATM and is known to cleave ubiquitin from IκB, thus dampening the NF-κB response (see below)."
19489724
rlimsp
"(B and C) Phosphorylated forms of both REST (B) and USP15 (C) accumulate during prometaphase arrest."
23708518
sparser
"To examine how USP15 phosphorylation affects cellular sensitivity to DNA damage, we reconstituted USP15-KO cells with ectopically expressed WT USP15 or USP15 S678A mutant."
30874560
sparser
"The expression of phosphorylated USP15 in breast cancer tissues was higher than that in normal tissues. ( p = 6.87e − 04) ( xref ), the phosphorylation expression of USP15 in different stages of breast cancer was also analyzed."
36213818
sparser
"USP15 phosphorylation is essential for its functions in HR."
30874560
sparser
"Mechanisms to control USP15 activity within cells are suggested by evidence that USP15 is alternatively spliced [ xref , xref ] and can be ubiquitylated or phosphorylated [ xref , xref , xref – xref ]."
29429988
sparser
"For instance, the FHA domain of MDC1 (mediator of DNA damage checkpoint protein 1) was found responsible for the recruitment of phosphorylated USP15 to double-stranded DNA breaks [114]."
34989786
sparser
"A recent study showed that the differential phosphorylation of USP15 on the UBL region (Thr149 and Thr219) results in an altered interaction with SART3 that affects USP15 localization and activity toward its substrate [ xref ]."
33946990
sparser
"No phospho-SQ/TQ signal was detected in Atm -deficient mouse embryonic fibroblasts (MEFs) (Fig.  xref ), suggesting that USP15 is phosphorylated in an ATM-dependent manner."
30874560
sparser
"Using the UALCAN database, we studied the expression of USP15 phosphorylation in breast cancer and normal tissues."
36213818
sparser
"Moreover, USP15 is phosphorylated during the cell cycle to regulate the mitotic degradation of the RE1-silencing transcription factor (REST) and subsequently dephosphorylated in early G1 while REST is stabilized and re-accumulated [ xref ]."
32512887
rlimsp
"Interestingly, we observed that a proportion of USP15 became phosphorylated at mitosis, which could conceivably alter its activity toward REST."
23708518
rlimsp
"We noticed that USP15 levels accumulate along with REST during G2; however, prometaphase arrest disengaged their correlation (Fig. 6A) co-incident with the appearance of a phosphorylated form of USP15 that persisted during mitosis (Fig. 6C)."
23708518