IndraLab

"As previously shown, the drug release kinetics into 10 mL 1 × PBS differ between the two wound dressings, although no differences in the disk diffusion assay (release into the gel) were seen. xref Interestingly, previously reported biofilm assays revealed that the CAM-PCL dressing was more effective compared with the CAM-PCL/PEO dressing on E. coli CFT073, most likely due to the different release kinetics. xref In the current assay, both formulations with the same drug load exhibited a similar antibiofilm effect, as a 6-log difference was observed in bacterial numbers for E. coli and S. epidermidis ( xref A,C)."

"The CAM-PCL/PEO dressing was statistically more effective in inhibiting biofilm formation than the CAM-PCL dressing."

"PCL-CIP fibrous wound dressings completely prevented the development of S. aureus and E. coli biofilm, as no viable bacteria were detected in the samples, in contrast to PCL-CAM fibrous wound dressing samples, which occasionally revealed low bacterial counts ( xref A–C, D)."

"As shown before for the CAM-PCL/PEO dressing, 90% of the drug is released in the first 60 min, but for the CAM-PCL dressing, the amount of drug released is only 40% after 24 h and up to 50% after 48 h. xref Therefore, the available drug concentration after 24 h of treatment is different, being higher when using the CAM-PCL/PEO dressing."

"Differences between formulations were also observed: the inhibition of biofilm bacteria was more significant for CAM-PCL/PEO than for CAM-PCL wound dressings ( xref )."

"Strong interactions between PCL and CAM together with hydrophobicity of the system resulted in much slower drug release compared to the hydrophilic ternary system of PCL/PEO/CAM."

"After treatment of S. aureus and E. coli 24-h biofilms with PCL-CAM fibrous wound dressing or CAM solution, no significant decrease in bacterial counts was seen ( xref D, E)."

"Drug encapsulation can either increase or decrease the mechanical properties of electrospun PCL fibers. xref , xref It has been shown that incorporation of the antibacterial drug linezolid into PCL scaffolds improved their mechanical properties. xref It is known that drug–polymer interactions affect the mechanical properties of electrospun polyester fibers, xref and as the solid-state analyses in our previous study have revealed, different solvents may have affected the CAM–PCL interactions at the molecular level differently. xref This may lead to different mechanical behavior of scaffolds."

"Previously developed and characterized fibrous wound dressings were used, namely PCL-CAM and pristine PCL (ES using a chloroform–methanol solvent system), xref and PCL-CIP and pristine PCL (ES using a formic acid–acetic acid solvent system), slightly modified from Zupančič et al. xref Different antibiotic-loaded dressings enabled the comparison of the antibacterial efficacy of the dressings with different drugs against dual-species wound infections (CAM vs CIP) and the effect of fibers with different morphologies."

"PCL-CAM wound dressing was tested only on S. aureus and E. coli biofilm due to the P. aeruginosa resistance to CAM. xref PCL-CIP dressing was tested on all three dual-species biofilm combinations: S. aureus with E. coli , S. aureus with P. aeruginosa , and E. coli with P. aeruginosa ."

"The results showed that PCL-CAM fibrous dressings were effective against the formation of dual-species biofilms at 24 and 48 h in S. aureus and E. coli within a dual-species biofilm model ( xref D–E)."

"In some cases, a small number of bacteria were detected even when a PCL-CAM wound dressing or CAM solution was applied."

"The ES solution compositions, conditions, and method parameters of all four different types of wound dressings (two pristine PCL and PCL/PEO dressings and two antibacterial drug CAM-loaded CAM-PCL and CAM-PCL/PEO dressings) have been previously published by Preem et al. In brief, ES solutions were prepared by dissolving the polymer PCL 12.5% (w/V) alone or PCL 10% (w/V) together with PEO 2% (w/V) in a chloroform/methanol (CHF/MET) (3:1 V/V) solvent mixture."

"No significant difference was found between the efficacy of the CAM solution and PCL-CAM fibers."

"CAM-loaded ES wound dressings (CAM-PCL and CAM-PCL/PEO) and pristine wound dressings (PCL and PCL/PEO) were cut into 1 × 1 cm samples under aseptic conditions under a laminar-flow hood."

"In a similar experiment, where the PCL-CAM fibrous wound dressing was used against a single-bacterium biofilm, no bacterial growth was detected after 24 or 48 h. xref "