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STAMBP binds STAM and SH3 domain. 3 / 3
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"To probe this, we used a combination of biophysical techniques and biochemical assays to assess three individual events : (1) AMSH recruitment to STAM via the SH3 domain and a longer STAM segment in which the UIM is fused to the SH3 domain (UIM-SH3), (2) ubiquitin binding to UIM-SH3, and finally, (3) the ternary complex of the catalytic domain of AMSH, UIM-SH3, and Lys63 linked diubiquitin."
24151880
reach
"We determined that the SH3 domain of STAM binds the catalytic domain of AMSH (AMSH219 E280A) with a K D of 1.4 +/- 0.04 microM (XREF_TABLE and XREF_SUPPLEMENTARY)."
24151880
reach
"The molecular mechanism underlying these opposing effects is hinted at by the observation that USP8 and AMSH bind a central SH3 domain of STAM proteins in a mutually exclusive manner."
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