A database built with INDRA combining content from numerous readers and databases. This page allows you to curate the loaded statements. For more information please see the manual.

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Statements

databases
phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
reading

PDPK1 phosphorylates AKT1 on T308. 10 / 58
1 14 6 6 | 7 3 9 12
biopax:pid
No evidence text available
hprd
No evidence text available
biopax:pid
No evidence text available
rlimsp
"Expressed PDK1 and DSTPK61 phosphorylated Thr308 of PKB alpha only in the presence of Ptdlns(3,4,5)P3 or Ptdlns(3,4)P2."
reach
"PDK1 phosphorylates Akt1 and Akt2 at residues Thr308 and Thr309, respectively."
signor
"Akt1 and akt2 are phosphorylated and activated by the protein kinase pdk1 at thr-308 or thr-309, respectively, in the activation t-loop, and further activation occurs through phosphorylation at ser-473 or ser-474, respectively. In this paper, we demonstrate that this is indeed the case, and report the purification and initial characterization of a 3 phosphoinositide-dependent protein kinase, pdk1, which activates pkb by phosphorylating it at thr308. Akt is directly phosphorylated and activated by pdk1. Akt/pkb activation requires the phosphorylation of thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (pdk1)."
hprd
No evidence text available
rlimsp
"On the contrary, Akt1 T308A accumulated in mitochondria and did not translocate to but rather decreased in nuclei suggesting that complete activation of Akt1 and further shuttle to nuclei depend on the phosphorylation in Thr308 by mitochondrial PDK1 (Fig. 4A)."
biopax:pid
No evidence text available
signor
"Protein kinase b (pkb) is activated by phosphorylation of thr308 and of ser473. Thr308 is phosphorylated by the 3-phosphoinositide-dependent protein kinase-1 (pdk1) but the identity of the kinase that phosphorylates ser473 (provisionally termed pdk2) is unknown."
PDPK1 phosphorylates AKT1. 10 / 43
3 | 6 5 15 14
sparser
"Mechanistically, PDK1 phosphorylates AKT1 in a critical activation site at T308 (T309 in AKT2) in the activation loop of the kinase domain, while mTORC2 phosphorylates AKT on S473 (S474 in AKT2) in the C-terminal hydrophobic motif [ xref , xref ]."
sparser
"The mTOR-rictor complex (mTORC2) phosphorylates AKT1 within the carboxy terminus at S473 and PDK1 phosphorylates AKT1 within its activation loop at T308 [ xref , xref ]."
reach
"Mechanistically, PDK1 phosphorylates AKT1 in a critical activation site at T308 (T309 in AKT2) in the activation loop of the kinase domain, while mTORC2 phosphorylates AKT on S473 (S474 in AKT2) in the C-terminal hydrophobic motif [XREF_BIBR, XREF_BIBR]."
rlimsp
"PI3K-independent AKT1 phosphorylation and activation by PDK1."
sparser
"To assess Akt1 mitochondria-nucleus functional connection and the significance of intramitochondrial Akt1 phosphorylation by PDK1, we obtained Akt1 T308A by directed mutagenesis, which renders a non-phosphorylatable mutant at Thr 308 ."
sparser
"Akt1 is phosphorylated by PDK1, and thereby activated upon translocation to the membrane [22,23] ."
sparser
"In summary, we have employed PCA as a novel tool to characterize AKT1 phosphorylation by PDK1 and provided direct evidence showing stabilized AKT1 association with PDK1 by the reconstituted IFP is sufficient for AKT1 phosphorylation by PDK1 independent of PI3K and membrane localization."
reach
"PIP3 generates membrane docking sites for both Phosphotidylinositol Dependent Kinase 1 (PDK1) and for the serine/threonine protein kinase AKT-1 through their pleckstrin homology domains, where PDK1 phosphorylates and activates AKT-1."
sparser
"Therefore, after phosphorylation of Akt1 by the PDK1 at the membrane, a conformational change occurs ( xref ), and Akt1 detaches from the membrane, leaving less Akt1 on the membrane at the time of observation."
sparser
"In contrast, the PIF-binding pocket of PDK1 is not required for the phosphorylation of PKBalpha by PDK1."
PDPK1 phosphorylates AKT1 on S473. 10 / 13
1 7 1 3 | 1
biopax:pid
No evidence text available
biopax:phosphositeplus
No evidence text available
biopax:netpath
No evidence text available
sparser
"It has been shown that PI3,4P2 and PI3,4,5P3, interacting with the PH domain of AKT-1/PKB, promote its translocation to the plasma membrane inducing conformational changes and, in parallel, activate P[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
signor
"We have partially purified a kinase from brain extract that phosphorylates Ser473 of PKBalpha in a PtdIns(3,4,5)P3-dependent manner and that is immunoprecipitated with PDK1 antibodies."
hprd
No evidence text available
PDPK1 phosphorylated on S241 phosphorylates AKT1 on T308. 9 / 9
9 |
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
PDPK1 phosphorylates AKT1 at position 308. 9 / 9
9 |
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
PDPK1 phosphorylates AKT1 on Y474. 6 / 6
6 |
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
PDPK1 phosphorylated on S241 phosphorylates AKT1 on S473. 3 / 3
3 |
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
PDPK1 phosphorylates AKT1 at position 473. 2 / 2
2 |
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
PDPK1 phosphorylates AKT1 on threonine. 2 / 2
| 1 1
reach
"PDK1 phosphorylates Akt1 at Thr XREF_BIBR, suggesting that another protein kinase, namely PDK2, is responsible for Ser 473 phosphorylation."
rlimsp
"The PI3K-dependent Akt serine/threonine phosphorylation by PDK1 and mTORC2 has long been thought to be the primary mechanism accounting for Akt activation."
PDPK1 phosphorylated on S241 phosphorylates AKT1 on Y474. 1 / 1
1 |
biopax:pid
No evidence text available
Phosphorylated PDPK1 phosphorylates AKT1. 1 / 1
1 |
biopax:panther
No evidence text available
PDPK1 bound to PI(3,4,5)P3 phosphorylates AKT1. 1 / 1
1 |
biopax:panther
No evidence text available
Modified PDPK1 leads to the phosphorylation of AKT1 on T308. 1 / 1
| 1
reach
"Overexpression of PDK1 in 293 cells activated PKB alpha and potentiated the IGF1 induced phosphorylation of PKB alpha at Thr308."