IndraLab

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PDPK1 phosphorylates AKT1 on T308. 65 / 68
12 1 6 6 2 | 17 16 5
hprd
No evidence text available
10226025
rlimsp
"Expressed PDK1 and DSTPK61 phosphorylated Thr308 of PKB alpha only in the presence of Ptdlns(3,4,5)P3 or Ptdlns(3,4)P2."
9368760
biopax:pid
No evidence text available
12242303
biopax:pid
No evidence text available
15550174
biopax:pid
No evidence text available
7637810
hprd
No evidence text available
10226025
reach
"Thr 308 of PKBalpha and Thr 252 of p70 S6 kinase are phosphorylated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) in vitro."
10601311
sparser
"Inactivation of GSK-3β Y216 was followed by the phosphorylation of Akt1 T308 by PDK1."
20041157
reach
"Phosphoinositide-dependent-protein kinase 1 (PDK1) phosphorylates AKT1 at threonine 308, and the second phosphorylation takes place at serine 473 by mTOR complex 2."
33815613
sparser
"In the cell-free assay Li2CO3 significantly inhibited phosphoinositide 3-kinase (PI3K)-mediated phosphorylation of Akt1 at Ser473, but Li2CO3 did not affect PI3K-mediated PI(3,4,5)P3 production and 3-phosphoinositide-dependent protein kinase 1 (PDK1)-mediated phosphorylation of Akt1 at Thr308."
24950409
reach
"Upon PI3K activation and/or PTEN inactivation, PDPK1 is recruited to the plasma membrane and phosphorylates AKT1 at threonine 308 (AKT1-T308), thus leading to its activation [XREF_BIBR]."
33544134
reach
"In contrast, PDK1 (1mug/ml) phosphorylated Akt1 only at Thr308, which was abolished by BX912 (100nM) (XREF_FIG B)."
24169049
biopax:pid
No evidence text available
16798838
sparser
"In contrast, PDK1 phosphorylates Akt1 at Thr308 and Akt2 at Thr309."
24169049
sparser
"A straightforward scaffold model predicted the dependence of AKT1 interaction with LASP1 at the cell membrane, potentially in combination with mTORC2, as AKT1 phosphorylation at T308 by PDK1 was not affected ( xref )."
32075106
reach
"PDK1 phosphorylates Akt1 and Akt2 at residues Thr308 and Thr309, respectively."
26937223
hprd
No evidence text available
9368760
sparser
"It has been shown that PI3,4P2 and PI3,4,5P3, interacting with the PH domain of AKT-1/PKB, promote its translocation to the plasma membrane inducing conformational changes and, in parallel, activate P[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11369519
reach
"PDK1 phosphorylates Thr 308 of PKBalpha (homologous phosphorylation sites are Thr 309 in PKBbeta and Thr 305 in PKBgamma), although full activation requires additional phosphorylation of Ser 473 (Ser [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11384738
sparser
"This pathway is initiated by receptor tyrosine kinase (RTK) or G-protein coupled receptor activation, like CXCR4, thus inducing phosphorylation of PIP2 to PIP3 by PI3K, thereby recruiting AKT1 and phosphoinositide-dependent kinase (PDK1) to the plasma membrane, where AKT1 is phosphorylated by PDK1 at T308."
32075106
reach
"Expressed PDK1 and DSTPK61 phosphorylated Thr308 of PKB alpha only in the presence of Ptdlns (3,4,5) P3 or Ptdlns (3,4) P2."
9368760
rlimsp
"In contrast, PDK1 (1 μg/ml) phosphorylated Akt1 only at Thr308, which was abolished by BX912 (100 nM) (Fig. 6B)."
24169049
biopax:pid
No evidence text available
9094314
sparser
"The phosphoinositide-dependent protein kinase 1 (PDK1) phosphorylates AKT1 on Thr308, which is required for AKT activity, but maximal activation of AKT also requires phosphorylation of Ser473 ( xref )."
30930055
biopax:pid
No evidence text available
hprd
No evidence text available
9368760
biopax:pid
No evidence text available
19401591
sparser
"The results also show that PI3K phosphorylates Akt1 both at Thr308 and Ser473 and Akt2 at Ser474 and that PDK1, a downstream effector of PI3K, phosphorylates Akt1 and Akt2 at Thr308 and Thr309 respectively."
24169049
reach
"Akt1 is phosphorylated by PDK1 at T308, and additionally at S473 by the Pl3K related kinases, mammalian target of rapamycin complex 2 (mTORC2) or DNA-PK to achieve its full kinase activity, depending on the stimulus and the cellular context."
22272775
sparser
"Akt1 is phosphorylated by PDK1 at T308, and additionally at S473 by the Pl3K-related kinases, mammalian target of rapamycin complex 2 (mTORC2) or DNA-PK to achieve its full kinase activity, depending on the stimulus and the cellular context ( xref )."
22272775
signor
"Akt1 and akt2 are phosphorylated and activated by the protein kinase pdk1 at thr-308 or thr-309, respectively, in the activation t-loop, and further activation occurs through phosphorylation at ser-473 or ser-474, respectively. In this paper, we demonstrate that this is indeed the case, and report the purification and initial characterization of a 3 phosphoinositide-dependent protein kinase, pdk1, which activates pkb by phosphorylating it at thr308. Akt is directly phosphorylated and activated by pdk1. Akt/pkb activation requires the phosphorylation of thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (pdk1)."
12808134
signor
"Protein kinase b (pkb) is activated by phosphorylation of thr308 and of ser473. Thr308 is phosphorylated by the 3-phosphoinositide-dependent protein kinase-1 (pdk1) but the identity of the kinase that phosphorylates ser473 (provisionally termed pdk2) is unknown."
10226025
reach
"The results show that the physical association of PDK1 with AKT1 induced by reconstituted IFP is sufficient to produce T308 phosphorylation and activation of AKT1 independent of PI3K activation or membrane localization."
20361045
sparser
"AKT1 T308 is phosphorylated by the upstream kinase PDK1."
35269443
reach
"This pathway is initiated by receptor tyrosine kinase (RTK) or G-protein coupled receptor activation, like CXCR4, thus inducing phosphorylation of PIP2 to PIP3 by PI3K, thereby recruiting AKT1 and phosphoinositide-dependent kinase (PDK1) to the plasma membrane, where AKT1 is phosphorylated by PDK1 at T308."
32075106
reach
"In the cell-free assay Li2CO3 significantly inhibited phosphoinositide 3-kinase (PI3K)-mediated phosphorylation of Akt1 at Ser473, but Li2CO3 did not affect PI3K mediated PI (3,4,5) P3 production and 3-phosphoinositide-dependent protein kinase 1 (PDK1)-mediated phosphorylation of Akt1 at Thr308."
24950409
phosphoelm
No evidence text available
9868760
rlimsp
"The results also show that PI3K phosphorylates Akt1 both at Thr308 and Ser473 and Akt2 at Ser474 and that PDK1, a downstream effector of PI3K, phosphorylates Akt1 and Akt2 at Thr308 and Thr309 respectively."
24169049
sparser
"PDK1 phosphorylates Akt1 and Akt2 at residues Thr308 and Thr309, respectively."
26937223
reach
"In contrast, PDK1 phosphorylates Akt1 at Thr308 and Akt2 at Thr309."
24169049
phosphoelm
No evidence text available
15718470
biopax:pid
No evidence text available
9094314
signor
"Pip3 acts in turn as a docking site for two kinases, phosphoinositidedependent kinase 1 (pdk1) and akt, and the subsequent phosphorylation of akt at serine 308 by pdk1, leading to akt activation."
21798082
sparser
"Another example of a scaffolding circRNA is circAMOTL1, which binds to pyruvate dehydrogenase kinase 1 (PDK1) and AKT serine/threonine kinase 1 (AKT1), leading to AKT1 phosphorylation at T308 by PDK1 and nuclear translocation, where it exerts antiapoptotic and differentiation functions xref ."
35013342
sparser
"Upon PI3K activation and/or PTEN inactivation, PDPK1 is recruited to the plasma membrane and phosphorylates AKT1 at threonine 308 (AKT1–T308), thus leading to its activation [ xref ]."
33544134
signor
"PIP3 recruits PDK1 and AKT to the plasma membrane, where PDK1 phosphorylates AKT on Thr308 in the activation loop of the kinase domain."
19951971
biopax:pid
No evidence text available
9094314
biopax:pid
No evidence text available
12538620
biopax:pid
No evidence text available
16798838
hprd
No evidence text available
10226025
reach
"In general, receptor- or non-receptor tyrosine kinases, PI3K, the 3-phosphoinositide-dependent protein kinase 1 (PDK1) and mTOR complex 2 (mTORC2) can activate Akt by promoting the phosphorylation of Akt1, Akt2 and Akt3 at residues T308/S473, T309/S474 and T305/S472, respectively [83]."
34419139
reach
"The results also show that PI3K phosphorylates Akt1 both at Thr308 and Ser473 and Akt2 at Ser474 and that PDK1, a downstream effector of PI3K, phosphorylates Akt1 and Akt2 at Thr308 and Thr309 respectively."
24169049
hprd
No evidence text available
9368760
biopax:pid
No evidence text available
16787925
biopax:phosphositeplus
No evidence text available
rlimsp
"In the cell-free assay, PI3K phosphorylated Akt1 both at Thr308 and Ser473, but PDK1 phosphorylated Akt1 only at Thr308."
24169049
reach
"Given that PDK1 specifically phosphorylates Akt1 at the Thr308 residue, and that Thr308 phosphorylation is lower in suicide brain, there is a strong possibility that decreased phosphorylation of Thr308 is due to diminished phosphorylation and activation of PDK1."
20163786
rlimsp
"Thr308 is phosphorylated by the 3-phosphoinositide-dependent protein kinase-1 (PDK1) but the identity of the kinase that phosphorylates Ser473 (provisionally termed PDK2) is unknown. RESULTS: The kinase domain of PDK1 interacts with a region of protein kinase C-related kinase-2 (PRK2), termed the PDK1-interacting fragment (PIF). PIF is situated carboxy-terminal to the kinase domain of PRK2, and contains a consensus motif for phosphorylation by PDK2 similar to that found in PKBalpha, except that the residue equivalent to Ser473 is aspartic acid. Mutation of any of the conserved residues in the PDK2 motif of PIF prevented interaction of PIF with PDK1. Remarkably, interaction of PDK1 with PIF, or with a synthetic peptide encompassing the PDK2 consensus sequence of PIF, converted PDK1 from an enzyme that could phosphorylate only Thr308 of PKBalpha to one that phosphorylates both Thr308 and Ser473 of PKBalpha in a manner dependent on phosphatidylinositol (3,4,5) trisphosphate (PtdIns(3,4,5)P3)."
10226025
sparser
"In contrast, PDK1 (1 μg/ml) phosphorylated Akt1 only at Thr308, which was abolished by BX912 (100 nM) ( xref B)."
24169049
signor
"Together, these results suggest a mechanism in which 3' phosphoinositide lipid-dependent translocation of pkb to the plasma membrane promotes serine 473 phosphorylation, which is, in turn, necessary for pdk1-mediated phosphorylation of threonine 308 and, consequentially, full pkb activation."
12167717
reach
"In the case of AKT, activated PI3 kinase phosphorylates (activating) the downstream protein PIP 3 -dependent kinase 1 (PDK1), which in turn phosphorylates protein kinase B (AKT) at a threonine 308 residue."
20161206
sparser
"In the cell-free assay, PI3K phosphorylated Akt1 both at Thr308 and Ser473, but PDK1 phosphorylated Akt1 only at Thr308."
24169049
reach
"AKT1 is usually activated by two different upstream kinases : PDPK1 phosphorylates AKT1 at the T308 residue, while the mTORC2 kinase complex phosphorylates the AKT1-S473 and AKT1-T450 sites."
25582703
sparser
"AKT1 is usually activated by two different upstream kinases: PDPK1 phosphorylates AKT1 at the T308 residue, while the mTORC2 kinase complex phosphorylates the AKT1-S473 and AKT1-T450 sites ( xref , xref )."
25582703
signor
"Akt/PKB activation requires the phosphorylation of Thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (PDK1) and Ser473 within the carboxyl-terminal hydrophobic motif by an unknown kinase."
15718470