IndraLab

Statements

USPL1 affects SUMO3
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USPL1 binds SUMO3.
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SUMO3 binds USPL1. 2 / 6
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"Here, we present the crystal structure of USPL1 bound to SUMO2, defining the key structural elements for the unusual deSUMOylase activity of USPL1."
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"To address these questions, we first established whether USPL1 in addition to catalysis also binds to SUMO2 preferentially."
37712884
USPL1 activates SUMO3.
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USPL1 activates SUMO3. 1 / 1
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"As shown in XREF_FIG C and 1D, SENP1, SENP2, SENP6, andSENP7, as well as USPL1, efficiently promoted global deconjugation of SUMO1 and SUMO2 from target substrates."
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USPL1 affects LEC
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USPL1 binds LEC. 5 / 5
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sparser
"USPL1 also interacts with ICE1, ELL, and EAF1 in PPID data, suggesting there is a physical interaction between USPL1 and the LEC."
35737447
sparser
"USPL1 interacts with LEC components involved in RNAPII-mediated snRNA transcription."
24413172
sparser
"Using a combination of fluorescence microscopy, molecular biology and proteomic approaches, we demonstrate that USPL1 interacts with components of the RNAPII-associated LEC and is associated with the U1 and U2 snRNA gene loci."
24413172
sparser
"To confirm the interaction of USPL1 with members of the LEC, we first used transient transfection of FLAG–ELL and HA–USPL1."
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sparser
"Our findings, together with other data ( xref ), indicate an association of USPL1 with components of the LEC."
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CCL16 affects USPL1
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CCL16 binds USPL1.
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USPL1 binds CCL16. 2 / 2
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"The strongest DepMap co-dependent genes for USPL1 across tumor types were members of the Little Elongation Complex (LEC), which is involved in transcription of spliceosomal machinery (Hutten et al., 2014) suggestive of an association between USPL1 and the LEC (Figure 3b)."
35737447
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"USPL1 also interacts with ICE1, ELL, and EAF1 in PPID data, suggesting there is a physical interaction between USPL1 and the LEC."
35737447
CCL16 activates USPL1.
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CCL16 activates USPL1. 1 / 1
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"The high degree of similarity between the USPL1 and ICE1 knockouts is consistent with the DepMap prediction that USPL1 activity is mediated largely by the LEC."
35737447
COIL affects USPL1
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COIL increases the amount of USPL1.
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COIL increases the amount of USPL1. 1 / 1
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"The overexpression of COIL genes considerably elevated the expression of FBL, ATXN1, WRAP53, and USPL1 genes compared with the control group, as was the expression of SMN, NOLC1, CBS, and KCNG1 genes."
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COIL binds USPL1.
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COIL binds USPL1 and foci. 1 / 1
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"Upon knockdown of coilin, USPL1 still formed nuclear foci, similar to SMN ( xref , arrows in middle panel)."
24413172
SnRNA gene loci affects USPL1
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USPL1 binds snRNA gene loci. 1 / 1
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"Therefore, we decided to investigate whether USPL1 itself could be found associated with snRNA gene loci."
24413172
Smo-1 affects USPL1
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Smo-1 binds USPL1. 1 / 1
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"We therefore examined the interaction of SUMO and USPL1 in more detail.A curated sequence alignment of USPL1 sequences of 183 organisms suggests that recognition of SUMO by USPL1 is based on a highly conserved patch in the finger subdomain, while other surface areas, with the exception of the surroundings of the catalytic center, are much more variable (Figure 3D)."
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Foci affects USPL1
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COIL binds USPL1 and foci. 1 / 1
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sparser
"Upon knockdown of coilin, USPL1 still formed nuclear foci, similar to SMN ( xref , arrows in middle panel)."
24413172
USPL1 affects snRNA gene loci
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USPL1 binds snRNA gene loci. 1 / 1
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"Therefore, we decided to investigate whether USPL1 itself could be found associated with snRNA gene loci."
24413172
USPL1 affects smo-1
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Smo-1 binds USPL1. 1 / 1
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"We therefore examined the interaction of SUMO and USPL1 in more detail.A curated sequence alignment of USPL1 sequences of 183 organisms suggests that recognition of SUMO by USPL1 is based on a highly conserved patch in the finger subdomain, while other surface areas, with the exception of the surroundings of the catalytic center, are much more variable (Figure 3D)."
37712884
USPL1 affects localization
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USPL1 inhibits localization. 1 / 1
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"Consistently, the levels of SUMOylated mCherry–FLAG-INTS11 WT decreased by overexpressing GFP–USPL1 or GFP–SENP6 (Supplementary Figure S7E), increasing the INTS11 cytoplasmatic localization (Figure 7A, B)."
36454007
USPL1 affects gus
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USPL1 increases the amount of gus. 1 / 1
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"AtUSPL1 expression was also assayed by tracking GUS expression produced by the pAtUSPL1 : : GUS transgene."
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USPL1 affects foci
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COIL binds USPL1 and foci. 1 / 1
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sparser
"Upon knockdown of coilin, USPL1 still formed nuclear foci, similar to SMN ( xref , arrows in middle panel)."
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"Our data suggest that USPL1 is involved in snRNA transcription and support the view that Cajal bodies have a role in snRNP biogenesis."
24413172
USPL1 affects Ubiquitin
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USPL1 desumoylates Ubiquitin. 1 / 1
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"It has recently been found that SUMO protease includes desumoylating isopeptidase 1 (DESI1), desumoylating isopeptidase 2 (DESI2) and ubiquitin specific protease like 1 (USPL1)."
29484374
USPL1 affects U2 snRNPs
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USPL1 binds U2 snRNPs. 1 / 1
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"USPL1 also interacts with U1 and U2 snRNPs and its knockdown causes a reduction in snRNA levels [XREF_BIBR]."
28615474
USPL1 affects U2 gene
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USPL1 inhibits U2 gene. 1 / 1
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"The results obtained by using two different antibodies clearly show a significant reduction in RNAPII occupancy at the U1 and U2 gene regions in cells treated with siRNA against USPL1 (XREF_FIG and supplementary material Fig."
24413172
USPL1 affects TRIM5
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USPL1 desumoylates TRIM5. 1 / 1
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"Mechanistically, deSUMOylation of TRIM5α by the deSUMOylating enzyme USPL1 in Cajal bodies allows TRIM5α to be sequestered in the nucleus, leading to efficient type I interferon responses by the DNA sensor cGAS during retroviral infection."
38280996
USPL1 affects SUMO2-KG-TAMRA
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USPL1 binds SUMO2-KG-TAMRA. 1 / 1
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"USPL1 specifically bound SUMO2-KG-TAMRA as is evident from a concentration-dependent increase in fluorescence anisotropy but did not bind SUMO1- or Ub-KG-TAMRA (Figure S6F)."
37712884
USPL1 affects SUMO1
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USPL1 activates SUMO1. 1 / 1
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"As shown in XREF_FIG C and 1D, SENP1, SENP2, SENP6, andSENP7, as well as USPL1, efficiently promoted global deconjugation of SUMO1 and SUMO2 from target substrates."
28903035
USPL1 affects SUMO-2/3
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USPL1 activates SUMO-2/3. 1 / 1
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"Lastly, USPL1 preferably promotes SUMO-2/3 deconjugation and is located in Cajal bodies [XREF_BIBR, XREF_BIBR]."
32714579
USPL1 affects SUMO-2 deconjugation
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USPL1 activates SUMO-2 deconjugation. 1 / 1
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eidos
"Lastly , USPL1 preferably promotes SUMO-2 / 3 deconjugation and is located in Cajal bodies [ 30,31 ] ."
32714579
USPL1 affects RNAPII
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USPL1 increases the amount of RNAPII. 1 / 1
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"Efficient knockdown of USPL1 by RNAi leads to reduced RNAPII mediated snRNA transcription, diminished production of snRNPs and altered pre-mRNA splicing."
24413172
USPL1 affects Protease
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USPL1 desumoylates Protease. 1 / 1
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"It has recently been found that SUMO protease includes desumoylating isopeptidase 1 (DESI1), desumoylating isopeptidase 2 (DESI2) and ubiquitin specific protease like 1 (USPL1)."
29484374
USPL1 affects INTS11
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USPL1 increases the amount of INTS11. 1 / 1
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"Remarkably, the levels of INTS11 SUMOylation, but not those of INTS4 and INTS9, are modulated by USPL1 (Figure 2G–I; Supplementary Figure S2A)."
36454007
USPL1 affects ICE1
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USPL1 binds ICE1 and ELL. 1 / 1
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"However, USPL1 was not identified in our ELL1-3 or MLL-ELL purifications, so the generality of an USPL1, ICE1, and ELL complex remains to be determined."
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USPL1 affects HDAC2
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USPL1 activates HDAC2. 1 / 1
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"Whilst USP33 or USPL1 depletion increased the HDAC2 and HDAC1 ratio, we identified USP27X and the tumor suppressor BAP1 as DUBs whose depletion lead to the most significant decrease in the HDAC2 and HDAC1 ratio."
25970771
USPL1 affects HDAC1
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USPL1 activates HDAC1. 1 / 1
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"Whilst USP33 or USPL1 depletion increased the HDAC2 and HDAC1 ratio, we identified USP27X and the tumor suppressor BAP1 as DUBs whose depletion lead to the most significant decrease in the HDAC2 and HDAC1 ratio."
25970771
USPL1 affects ELL
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USPL1 binds ICE1 and ELL. 1 / 1
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"However, USPL1 was not identified in our ELL1-3 or MLL-ELL purifications, so the generality of an USPL1, ICE1, and ELL complex remains to be determined."
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"Importantly, we find that USPL1 associates directly with U snRNA loci and that it interacts and colocalises with components of the Little Elongation Complex, which is involved in RNAPII mediated snRNA transcription."
24413172
USPL1 affects DESI2
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USPL1 desumoylates DESI2. 1 / 1
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"It has recently been found that SUMO protease includes desumoylating isopeptidase 1 (DESI1), desumoylating isopeptidase 2 (DESI2) and ubiquitin specific protease like 1 (USPL1)."
29484374
USPL1 affects COIL
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COIL binds USPL1 and foci. 1 / 1
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sparser
"Upon knockdown of coilin, USPL1 still formed nuclear foci, similar to SMN ( xref , arrows in middle panel)."
24413172
USPL1 affects CBS
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USPL1 activates CBS. 1 / 1
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"When CBs were perturbed by siRNA knockdown of TCAB1 or USPL1, we could no longer observe the spatial clustering of previously examined sn/snoRNA loci and the histone loci (XREF_FIG)."
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USPL1 affects AtRD22
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USPL1 activates AtRD22. 1 / 1
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"In the root, AtUSPL1 transcription is stimulated by exposure to either mannitol or NaCl, as is that of AtRD22 in the leaf XREF_BIBR."
25333723
USPL1 affects -U2 pre-snRNA
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USPL1 inhibits -U2 pre-snRNA. 1 / 1
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"Depletion of the essential CB components TCAB1 and WRAP53 or USPL1, which both lead to complete CB disassembly, decreases nascent precursor U1 and -U2 pre-snRNA levels [XREF_BIBR], whereas coilin depletion, which does not completely abolish CBs but results in residual CB like substructures, increases pre-U snRNA levels [XREF_BIBR]."
27767214
U2 snRNPs affects USPL1
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USPL1 binds U2 snRNPs. 1 / 1
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"USPL1 also interacts with U1 and U2 snRNPs and its knockdown causes a reduction in snRNA levels [XREF_BIBR]."
28615474
SUMO2-KG-TAMRA affects USPL1
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USPL1 binds SUMO2-KG-TAMRA. 1 / 1
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"USPL1 specifically bound SUMO2-KG-TAMRA as is evident from a concentration-dependent increase in fluorescence anisotropy but did not bind SUMO1- or Ub-KG-TAMRA (Figure S6F)."
37712884
ICE1 affects USPL1
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USPL1 binds ICE1 and ELL. 1 / 1
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"However, USPL1 was not identified in our ELL1-3 or MLL-ELL purifications, so the generality of an USPL1, ICE1, and ELL complex remains to be determined."
22195968
DESI1 affects USPL1
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DESI1 desumoylates USPL1. 1 / 1
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"Moreover, recent studies have identified three new SUMO proteases in humans, desumoylating isopeptidase 1 (DeSI1), DeSI2 18 and ubiquitin specific protease like 1 (USPL1) 19, which share little sequence similarity with the Ulp and SENP protease class."
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COIL affects foci
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COIL binds USPL1 and foci. 1 / 1
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sparser
"Upon knockdown of coilin, USPL1 still formed nuclear foci, similar to SMN ( xref , arrows in middle panel)."
24413172
CARM1 affects USPL1
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CARM1 activates USPL1. 1 / 1
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"As expected, re-introduction of wt CARM1 into the shCARM1 MN-1 cells rescued the Uspl1 E2 +/E2- ratios, returning them towards levels observed in the control condition."
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