
IndraLab
Statements
reach
"Based on the convincing fit of the eIF3a and eIF3c structure into the EM density, we attempted to dock the recently solved paralogs of eIF3e (CSN1) and the eIF3h and eIF3f heterodimer (Rpn8 and Rpn11) into the eIF3.IRES EM density along with older models for eIF3k, eIF3m, and eIF3l (XREF_FIG A)."
sparser
"To test whether eIF3f and eIF3h actually interact with the eIF4GI682-1130-MS2 fusion protein, we co-expressed both factors transiently in HEK 293T cells, immunoprecipitated the eIF4G core domain via its MS2 fusion in presence of RNase A and tested for its association of eIF3f and eIF3h ( xref )."
sparser
"The subunit eIF3g interacts with Paip1, a Poly (A)-binding protein and stimulates translation initiation [ xref ], whereas the subunits eIF3h and eIF3f interact with TRC8, a ubiquitin E3 ligase, and inhibit protein synthesis, possibly through ubiquitilation of eIF3 or some other translational components [ xref ]."