IndraLab

Statements

MTOR is active.
3 9 | 1
MTOR phosphorylated on S2448 is active. 4 / 4
3 1 |
biopax:pid
No evidence text available
10499523
biopax:pid
No evidence text available
1614535
biopax:pid
No evidence text available
15051500
signor
"Importantly, phosphorylation of mTOR by S6K1 occurs at threonine 2446/serine 2448. This region has been shown previously to be part of a regulatory repressor domain. These sites are also constitutively phosphorylated in the breast cancer cell line MCF7 carrying an amplification of the S6K1 geneit has been proposed that other inputs, in addition to phosphorylation of Thr-2446/Ser-2448 by S6K1, are part of the mechanism involved in inhibiting this repressor domain"
15905173
MTOR bound to RHEB is active. 2 / 2
2 |
signor
"Rheb binds and regulates the mTOR kinase."
15854902
signor
"Rheb stimulates the phosphorylation of mtor and plays an essential role in regulation of s6k and 4ebp1 in response to nutrients and cellular energy status."
20006481
MTOR phosphorylated on T2446 is active. 2 / 2
2 |
signor
"We show that s6 kinase 1 (s6k1), but not akt, directly phosphorylates mtor in cell-free_ in vitro_ system and in cells."
12807916
signor
"Importantly, phosphorylation of mTOR by S6K1 occurs at threonine 2446/serine 2448. This region has been shown previously to be part of a regulatory repressor domain. These sites are also constitutively phosphorylated in the breast cancer cell line MCF7 carrying an amplification of the S6K1 geneit has been proposed that other inputs, in addition to phosphorylation of Thr-2446/Ser-2448 by S6K1, are part of the mechanism involved in inhibiting this repressor domain"
15905173
MTOR phosphorylated on S2481 is active. 2 / 2
2 |
signor
"We have found that in HEK293 cells and 3T3-L1 adipocytes, insulin promotes both raptor- and rictor-associated mTOR Ser(P)-2481 in a wortmannin-sensitive manner. Thus, insulin signals via PI3K to promote both mTORC1- and mTORC2-associated mTOR Ser-2481 autophosphorylation."
20022946
signor
"We report here the identification of a FRAP autophosphorylation site. This site, Ser-2481, is located in a hydrophobic region near the conserved carboxyl-terminal FRAP tail. We demonstrate that the COOH-terminal tail is required for FRAP kinase activity and for signaling to the translational regulator p70(s6k) (ribosomal subunit S6 kinase)."
10702316
MTOR bound to AC1NUST0 is active. 1 / 1
1 |
signor
"Pa directly interacted with the domain in mtor that is targeted by rapamycin, and this interaction was positively correlated with mtor's ability to activate downstream effectors."
11729323
MTOR bound to kinase activity is active. 1 / 1
| 1
trips
"In contrast to TRPP1 deficiency, TRPP2-deficient cells did neither display excessive activation of the mTOR-kinase complex nor inhibition of AMPK activity, while ERK1/2 and Akt activity were similarly affected among TRPP1- and TRPP2-deficient cells."
24193408
Phosphorylated MTOR is active. 1 / 1
1 |
signor
"Once phosphorylated, Akt can act on a broad spectrum of substrates that can influence cell survival and proliferation and protein synthesis (65). Phosphorylation of mTOR by Akt leads to mTOR activation (40, 52) and the subsequent activation of p70S6K"
15829723
MTOR is kinase-active.
3 2 |
MTOR phosphorylated on S2448 is kinase-active. 4 / 4
2 2 |
bel
"We first studied the effect of 12 h exposure to T3 on S2448 phosphorylation of mTOR to match the exposure time for Northern blot analysis (Fig. 1A). As shown in Fig. 1B, an increase in S2448 phosphorylation was detected after 12 h exposure to T3....The phosphorylation is associated with a marked increase in T389 phosphorylation of p70S6K. These results demonstrate T3-dependent phosphorylation of S2448 activates mTOR kinase."
15388791
bel
"In contrast to the effect of phosphorylation by Akt/PKB on most of its substrates, phosphorylation of mTOR on Ser2448 reportedly promotes mTOR-dependent signalling (Nave et al. 1999)."
15294054
bel
"In contrast to the effect of phosphorylation by Akt/PKB on most of its substrates, phosphorylation of mTOR on Ser2448 reportedly promotes mTOR-dependent signalling (Nave et al. 1999)."
15294054
bel
"We first studied the effect of 12 h exposure to T3 on S2448 phosphorylation of mTOR to match the exposure time for Northern blot analysis (Fig. 1A). As shown in Fig. 1B, an increase in S2448 phosphorylation was detected after 12 h exposure to T3....The phosphorylation is associated with a marked increase in T389 phosphorylation of p70S6K. These results demonstrate T3-dependent phosphorylation of S2448 activates mTOR kinase."
15388791
MTOR phosphorylated on S2481 is kinase-active. 1 / 1
1 |
bel
"Phosphorylation of wild-type but not kinase-inactive FRAP occurs at Ser-2481 in vivo, suggesting that Ser-2481 phosphorylation is a marker of FRAP autokinase activity in cells."
10702316
MTOR is inactive.
5 |
MTOR bound to DEPTOR is inactive. 1 / 1
1 |
signor
"DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival"
19446321
MTOR bound to FKBP8 is inactive. 1 / 1
1 |
signor
"Fkbp38 binds to mtor and inhibits its activity in a manner similar to that of the fkbp12-rapamycin complex."
17991864
MTOR phosphorylated on S2448 is inactive. 1 / 1
1 |
signor
"Although AKT phosphorylated mTOR at two COOH-terminal sites (Thr2446 and Ser2448) in vitro, Ser2448 was the major phosphorylation site in insulin-stimulated or -activated AKT-expressing human embryonic kidney cells. Transient transfection assays with mTOR mutants bearing Ala substitutions at Ser2448 and/or Thr2446 indicated that AKT-dependent mTOR phosphorylation was not essential for either PHAS-I phosphorylation or p70S6K activation in HEK cells."
10910062
Phosphorylated MTOR is inactive. 1 / 1
1 |
signor
"Abl binds directly to raft1 and phosphorylates raft1 in vitro and in vivo. c-abl inhibits autophosphorylation of raft1 and raft1-mediated phosphorylation p70(s6k)."
10753870
MTOR phosphorylated on T2446 is inactive. 1 / 1
1 |
signor
"AKT phosphorylated mTOR at two COOH-terminal sites (Thr2446 and Ser2448) in vitro, Ser2448 was the major phosphorylation site in insulin-stimulated or -activated AKT-expressing human embryonic kidney cells. These results demonstrate that mTOR is a direct target of the PI3K-AKT signaling pathway in mitogen-stimulated cells, and that the identified AKT phosphorylation sites are nested within a repressor domain that negatively regulates the catalytic activity of mTOR."
10910062