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USP10 binds G3BP1. 142 / 190
36 1 | 81 61
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"Resveratrol binds to G3BP1 and interrupts the G3BP1/USP10 interaction, releasing USP10 and promoting its deubiquitinating activity, increasing p53-mediated apoptosis in melanoma (Oi et al., 2015)."
34744708
sparser
"In addition, several viruses (Semliki Forest, Chikungunya, and herpes simplex) encode SG-inhibiting proteins that share the same binding motif (FGDF motif) within USP10 that binds to G3BP ( xref )."
30082464
sparser
"Through searching for protein–protein interaction (PPI) network and Gene Expression Profiling Interactive Analysis (GEPIA) website, we found that USP10 interacted with G3BP1, and that there was a positive correlation between G3BP1 mRNA expression and USP10 mRNA expression in OC."
34967276
sparser
"TAT-RasGAP 317–326 did not modulate binding of G3BP1 to USP10, stress granule formation or c-myc mRNA levels."
22205990
sparser
"We performed PPI analysis and found that G3BP1 interacted with USP10 (Fig.  xref A), and G3BP1 was involved in the ubiquitination of p53 by USP10 [ xref ]."
38243224
sparser
"G3BP1 interacts with USP10 in OC cells."
34967276
sparser
"To assess whether G3BP1 interacts with USP10 in OC or not, we used an IP assay to verify this interaction."
34967276
sparser
"Mechanistically, G3BP1 forms a complex with USP10 in the progression of OC."
34967276
sparser
"We next found that USP10 is a potent regulator of G3BP1 expression in OC cells, and confirmed that G3BP1 bound well to USP10 at endogenous level in OC cells."
34967276
sparser
"Caprin1 binding to G3BP promotes SG formation, whereas USP10 binding to G3BP inhibits SG assembly ( Kedersha et al., 2016 )."
27693920
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"Additionally, we found that G3BP1, a known binding partner of USP10, 27 is likewise depleted from senescent ribosomes ( Figure 3 I; Table S1 )."
37930887
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"Kedersha and colleagues 37 concluded that G3BP binds 40S ribosomes through its RGG region and is essential for stress granule condensation, but distinct from polysome disassembly, and the condensation process is regulated by competitive Caprin1 and USP10 binding to G3BP."
| PMC
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"It has been reported that the G3BP1 mutant is proficient for stress granule formation but fails to interact with USP10 whereas the G3BP1 is deficient for stress granule formation but still binds USP10 (Panas et al, 2015; Kedersha et al, 2016)."
36574355
sparser
"The binding of G3BP to USP10 appears to inhibit its deubiquitinating activity."
12672452
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"USP10, which also binds to G3BP but inhibits stress granule assembly was identified as a suppressor."
27768896
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"In addition, G3BP1-mediated SGs assembly is regulated by the mutually exclusive binding of Caprin1 and USP10, that is, Caprin1 binds to G3BP1 to promote SGs assembly, while USP10 binds to G3BP1 to inh[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35176431
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"Resveratrol (RSVL) directly binds to G3BP1 and prevents the interaction of G3BP1/USP10, which enhances the USP10-mediated de-ubiquitination of p53, thereby increasing p53 expression (135)."
34526993
sparser
"TAT-RasGAP 317–326 does not affect the binding of G3BP1 to USP10."
22205990
sparser
"The results suggested that G3BP1 bound well to USP10 at endogenous level ( xref )."
34967276
sparser
"In summary, these results imply that G3BP1 interacts with USP10 in OC cells."
34967276
reach
"Resveratrol binds to G3BP1 and blocks the G3BP1/USP10 interaction, enhancing USP10-mediated p53 deubiquitination and increasing p53 expression [24]."
38243224
sparser
"USP10 binds G3BP via a conserved FGDF motif."
25658430
sparser
"To determine whether this USP10 region binds G3BP, we fused the N-terminal 40 residues of human USP10 to EGFP (EGFP-USP10 1–40 )."
25658430
sparser
"To test if the overexpression and binding of full-length USP10 to G3BP affects the SG response, BHK cells were transfected with EGFP alone, EGFP-USP10 wt or EGFP-USP10-F10A. Transfected cells were mock stressed or stressed with sodium arsenite for 1 h before fixation and staining for G3BP-1 and TIA-1 to detect SGs."
25658430
sparser
"Mechanistically, DCAF7 serves as a scaffold protein that facilitates the interaction between USP10 and G3BP1, leading to the elimination of K48‐linked ubiquitin moieties from Lys76 of G3BP1."
38973296
sparser
"G3BP1 binds to USP10 and therefore disrupts the interaction of USP10 with p53, leading to suppression of p53 deubiquitination."
24998844
sparser
"Mechanistically, DCAF7 promoted the binding of USP10 to G3BP1, leading to the elimination of K48‐linked ubiquitin moieties from Lys76 of G3BP1, thus preventing the degradation of G3BP1 via the ubiquitin‒proteasome pathway and facilitating SG‐like structures to form."
38973296
sparser
"We then determined whether USP10 is recruited by DCAF7 to stabilize G3BP1 and found that DCAF7 deficiency markedly inhibited the interaction between USP10 and G3BP1 (Figure  xref ), indicating that DCAF7 is required for this interaction."
38973296
sparser
"Among these, the IDP hub protein G3BP1 can form complexes with cytoplasmic phosphoprotein Caprin1 and ubiquitin peptidase USP10; the resulting control of USP10 activity contributes to a pathogenic virulence system that targets endocytic recycling of the ion channel CFTR."
36183834
sparser
"Given that the interaction between G3BP1 and USP10 inhibits stress granule formation, this function is anticipated to be relevant to unstressed/physiological conditions, where translation rates are higher (Kedersha et al., xref )."
33349931
sparser
"However, for the first time we show an in vitro interaction of the purified NTF2-like domain of G3BP1 (residues 1–139) and the N-terminal region of USP10 (residues 1–150)."
24998844
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"Studies using USP10 mutants, including USP10/77 -792 and USP10/95-792, indicate that USP10 's interaction with G3BP1 is essential for inhibition of ROS dependent apoptosis and for USP10 incorporation into stress granules."
27974222
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"Thus, USP10 interaction with G3BP1 and the incorporation of USP10 into stress granules are dispensable for USP10 inhibition of cytokine deprivation induced apoptosis in FL HSPCs."
27974222
sparser
"Interestingly, recent work has shown that the compound resveratrol can inhibit the interaction of G3BP with USP10 by binding to the NTF2-like domain of G3BP, thereby stimulating USP10 DUB activity and stabilization of p53 [ xref ]."
25658430
reach
"The conclusions that G3BP binds 40S ribosomes through its RGG region and is essential for stress granule condensation, stress granule condensation is distinct from polysome disassembly, and the condensation process is regulated by competitive Caprin1/USP10 binding to G3BP still stand firmly."
31851327
sparser
"Two proteins—Caprin1 and USP10—bind to a region of G3BP neighboring this critical phosphorylation site."
31851330
sparser
"Kedersha et al. found that the proteins compete with each other for G3BP binding and that, whereas Caprin1 promotes SG formation, USP10’s interaction with G3BP inhibits SG assembly."
31851330
sparser
"G3BP1 binds to USP10 and inhibits the deubiquitination activity of USP10 ( xref )."
24998844
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"The FGDF motif of the mammalian ubiquitin-specific protease (USP10) binds to G3BP, thereby affecting the interaction of G3BP1/2 and Caprin1 to inhibit the formation of SGs (36)."
34526993
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"In the article, the authors suggested that G3BP-S149 phosphorylation alters the outcome of stress granule formation by preferentially promoting the binding of G3BP to USP10 relative to Caprin1, but the authors now know that the S149E construct instead failed to rescue stress granule formation because of the unexpected S99P mutation (as shown in the Letter [Panas et al., 2019."
31851327
sparser
"Interestingly, our previous map of human protein complexes ( xref ) suggests that the known complex of G3BP, CAPRIN, and USP10 ( xref ) also physically interacts with NUFIP2, leading us to suggest the name CapGUN (i.e., CAPRIN1, G3BP2, USP10, NUFIP2)."
31665645
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"In addition, we show that binding of the cellular G3BP binding partner USP10 is also mediated by an FGDF motif."
25658430
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"The binding of G3BP to USP10 appears to inhibit its deubiquitinating activity."
12672452
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"Moreover, they also provide a molecular basis for the regulation of USP10 activity by assembly of the inhibitory USP10 and G3BP complex."
25658430
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"USP10 binds G3BP via a conserved FGDF motif."
25658430
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"We wondered whether this interaction displaces USP10, a cellular binding partner of G3BP that also binds to the NTF2 like domain [XREF_BIBR, XREF_BIBR]."
25658430
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"To determine whether this USP10 region binds G3BP, we fused the N-terminal 40 residues of human USP10 to EGFP (EGFP-USP10 1-40)."
25658430
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"The USP10 and G3BP complex appears to co-immunoprecipitate with ubiquitinated species that could be substrates of USP10."
11439350
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"Since the binding of nsP3 and USP10 to G3BP inhibits SGs, we hypothesized that ICP8 binding might do the same."
25658430
sparser
"Caprin1 binding to G3BP promotes SG formation, whereas USP10 binding to G3BP inhibits SG assembly."
27022092
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"It appears therefore that the FGDF mediated G3BP and USP10 complex is mutually inhibitory, with G3BP inhibiting the DUB activity of USP10 and USP10 inhibiting the SG nucleating function of G3BP."
25658430
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"Our study illustrates that the USP10-G3BP1 complex acts as a hub that interacts with various pathways to adapt to environmental stimuli promptly."
39863576
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"As such, we highly suspect that the USP10-G3BP1 complex can talk to energy metabolic pathways during RQC.Mitochondria are the primary site for producing adenosine triphosphate (ATP), the cell's energy currency."
39863576
sparser
"In addition, G3BP1-mediated SGs assembly is regulated by the mutually exclusive binding of Caprin1 and USP10, that is, Caprin1 binds to G3BP1 to promote SGs assembly, while USP10 binds to G3BP1 to inh[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35176431
sparser
"Studies using USP10 mutants, including USP10/77–792 and USP10/95–792, indicate that USP10's interaction with G3BP1 is essential for inhibition of ROS-dependent apoptosis and for USP10 incorporation into stress granules ( xref )."
27974222
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"The G3BP1/USP10 complex immunoprecipated from human U2OS cells was more resistant to acidic buffer washes than G3BP1/Caprin-1."
37161291
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"In summary, it is believed that mTORC2 interacts with the USP10-G3BP1 complex and plays a significant role in governing mitochondrial morphology and function."
39863576
sparser
"Thus, USP10 interaction with G3BP1 and the incorporation of USP10 into stress granules are dispensable for USP10 inhibition of cytokine deprivation-induced apoptosis in FL HSPCs."
27974222
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"However, the precise region of FMRP that interacts with the USP10-G3BP1 complex remains unknown, highlighting the need for further investigation."
39863576
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"Further elucidation in biochemical analysis will determine if FMRP undergoes ubiquitination during RQC, and potentially acts as a target for the USP10-G3BP1 complex in subsequent molecular events."
39863576
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"Distant homologs of these proteins in mammals may provide insights into the complex role of the USP10-G3BP1 complex, but more extensive and in-depth studies are needed in the future."
39863576
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"Under normal conditions, G3BP1 binds to and inhibits USP10, but in stress, when both proteins become incorporated into SGs, their interaction changes and this unleashes the activity of USP10 XREF_BIBR."
25375155
sparser
"Cif stabilizes USP10 and G3BP1 interaction to inhibit USP10 activity."
21455491
sparser
"Three observations suggest that Cif may inhibit USP10 activity by stabilizing the interaction between USP10 and G3BP1, which inhibits USP10 DUB activity, and also reduces the interaction between USP10 and CFTR."
21455491
sparser
"First, published studies have shown that USP10 and G3BP1 interact in yeast and mammalian systems xref , xref , xref ."
21455491
sparser
"Second, in U2OS bladder cancer cells, the interaction between G3BP1 and USP10 inactivates the deubiquitinating enzyme activity of USP10 xref ."
21455491
sparser
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."
21455491
sparser
"As predicted, addition of G3Ib to these lysates resulted in a dose-dependent reduction of the interaction between G3BP1 and both endogenous caprin 1 and endogenous USP10, whereas the inactive enantiomer G3Ib’ had no such effect ( xref – xref )."
37425931
sparser
"To identify point mutations specifically targeting the N protein interaction, we excluded G3BP1 F33A/F33W because these mutations cause the loss of interaction with USP10 ( xref ) as well as G3BP1 F15A and F124A because these proteins were expressed at much lower levels, reflecting potential instability."
38492217
sparser
"Moreover, Cif increased the interaction between G3BP1 and USP10 by 210±12% ( xref )."
21455491
sparser
"Thus, these observations reveal that Cif stabilizes an interaction between USP10 and G3BP1, and also reduces the interaction between USP10 and CFTR."
21455491
sparser
"To provide additional support for the immunoprecipitation studies demonstrating that Cif increases the interaction between G3BP1 and USP10 in the early endosomal compartment, we performed bimolecular fluorescence complementation (BiFC) studies."
21455491
reach
"Mechanistically, DCAF7 serves as a scaffold protein that facilitates the interaction between USP10 and G3BP1, leading to the elimination of K48-linked ubiquitin moieties from Lys76 of G3BP1."
38973296
sparser
"Indeed, we have demonstrated that G3Ia and G3Ib inhibit the interaction of G3BP1 with caprin 1 and USP10 ( xref )."
37425931
sparser
"Moreover, by co-transfecting cells with organelle markers, it is possible to identify the compartment where USP10 and G3BP1 interact."
21455491
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"Cif stabilizes a G3BP1–USP10 complex in lung cells, which renders USP10 inactive and thus unable to deubiquitinate CFTR (20, 36, 75)."
36183834
sparser
"These studies support the conclusion that Cif stabilizes the interaction between USP10 and G3BP1 in the early endosomes."
21455491
sparser
"Taken together, these data confirm our hypothesis that Cif, by enhancing G3BP1 interaction with USP10, inhibits the ability of USP10 to interact with, and deubiquitinate CFTR."
21455491
sparser
"A previous study reported that G3BP1 interacts directly with the N-terminus of USP10, a common protein-protein interaction domain in the USP family of DUBs for regulation of DUB activity xref ."
21455491
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"These modifications align with the established role of the USP10-G3BP1 complex in the recycling of the 40S ribosomal subunit , and further validate the efficacy of our proteomic analysis approach."
39863576
sparser
"USP10, which also binds to G3BP but inhibits stress granule assembly ( xref ) was identified as a suppressor."
27768896
sparser
"Together, these data indicate that, while USP10 and its binding partner G3BP1 are bound to ribosomes of proliferating cells, the complex is specifically depleted from the ribosome interactome upon ind[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37930887
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"Cif does not alter the protein abundance of USP10, but does enhance the interaction between G3BP1 and USP10 to inhibit USP10 DUB activity."
21998879
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"We found that USP10, the human ortholog of Ubp3p, associates with the Bre5 ortholog G3BP1, as previously reported (XREF_SUPPLEMENTARY), and also associates with the G3BP1 paralog G3BP2 (XREF_FIG)."
19615732
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"USP10, another binding partner of G3BP1 [58], negatively regulates SGs assembly by masking the RNA multivalent binding site of G3BP1 [71]."
37906632
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"They showed that resveratrol directly binds to G3BP1 and disrupts the G3BP1/USP10 interaction, releasing USP10, which leads to enhanced USP10-mediated deubiquitination modification of p53, thereby rai[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35176431
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"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [41,90]."
30326201
reach
"TAT-RasGAP 317-326 did not modulate binding of G3BP1 to USP10, stress granule formation or c-myc mRNA levels."
22205990
reach
"TAT-RasGAP 317-326 does not affect the binding of G3BP1 to USP10."
22205990
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"Interestingly, protein interactions can also prevent SG core growth, as reported by the interaction between USP10/G3BP in mammals (Kedersha et al., 2016)."
34434210
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"The Ras GTPase-activating protein-binding protein 1 (G3BP1)-family-ubiquitin-specific peptidase 10 (USP10) complex is involved in degrading 40S ribosomal subunits in response to ribosome collision [88]."
38740259
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"Kedersha et al. found that the proteins compete with each other for G3BP binding and that, whereas Caprin1 promotes SG formation, USP10 's interaction with G3BP inhibits SG assembly."
31851330
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"The DUB USP10 binds G3BP, localizes to SGs and regulates SG formation, even though the exact role of its DUB activity in this process remains controversial."
33687997
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"Furthermore, Panas et al. found that USP10 bind to G3BP and inhibited the formation of SG downstream of polysome disassembly through its mediated motif Phe-Gly-Asp-Phe (FGDF) ( Kedersha et al., 2016 ;[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35461975
reach
"Among these, the IDP hub protein G3BP1 can form complexes with cytoplasmic phosphoprotein Caprin1 and ubiquitin peptidase USP10; the resulting control of USP10 activity contributes to a pathogenic virulence system that targets endocytic recycling of the ion channel CFTR."
36183834
reach
"When the degree of copurification for each mutant is plotted as a function of the associated K , it is evident that changes in binding affinity of G3BP1 NTF2 domains for the USP10 FGDF peptide correlate with changes in full-length G3BP1–USP10 complex stability (Fig. 2; blue)."
36183834
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"Physical interaction between USP10 and G3BP, a RasGAP interacting protein overexpressed in tumors, was shown by a two-hybrid screen to suggest a link between the ubiquitination pathway and Ras mediated signaling 61 More than half of the identified phosphoproteins were mapped to relate with cancer function."
22699815
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"Caprin1 binding to G3BP promotes SG formation, whereas USP10 binding to G3BP inhibits SG assembly."
27022092
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"USP10 and Caprin1 antagonistically bind G3BP to regulate SG formation."
27022092
sparser
"In contrast, binding of USP10 to G3BP promotes the soluble confirmation of G3BP, and thereby induces SG disassembly ( xref )."
33007331
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"Therefore, although Caprin1 and USP10 both bind G3BP, they have antagonistic effects on SGs."
27022092
reach
"G3BP1 binds to USP10 and therefore disrupts the interaction of USP10 with p53, leading to suppression of p53 deubiquitination."
24998844
sparser
"Furthermore, Panas et al. found that USP10 bind to G3BP and inhibited the formation of SG downstream of polysome disassembly through its mediated motif Phe-Gly-Asp-Phe (FGDF) ( Kedersha et al., 2016 ;[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35461975
sparser
"Caprin1 binding to G3BP promotes the formation of SGs, whereas the binding of USP10 to G3BP abolishes SG assembly."
33007331
sparser
"Previous studies suggested that Caprin-1 interacted with G3BP1 NTF2L via residues 352 to 380 with a low affinity ( xref ), and USP10 interacted with G3BP1 NTF2L via residues 1 to 40 ( xref ) ( xref )."
36279435
sparser
"Interestingly, neither Caprin1 nor USP10 binding to G3BP is needed for its association with the 40S subunit ( xref )."
33007331
sparser
"Physical interaction between USP10 and G3BP, a RasGAP interacting protein overexpressed in tumors, was shown by a two-hybrid screen to suggest a link between the ubiquitination pathway and Ras-mediated signaling xref "
22699815
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"Resveratrol, on the other hand, directly binds to G3BP1 and prevents the G3BP1 and USP10 interaction, resulting in enhanced USP10 mediated deubiquitination of p53 and consequently increased p53 expression."
24998844
reach
"Resveratrol, on the other hand, directly binds to G3BP1 and disrupts the G3BP1 and USP10 interaction, resulting in enhanced USP10 regulated deubiquitination of p53."
24998844
sparser
"The GIM of USP10 drastically reduced the number and sizes of G3BP1 droplets ( xref ), suggesting that the inhibitory effect of USP10 on G3BP1 LLPS is likely caused by the interaction between USP10 GIM and G3BP1."
36279435
reach
"We conjectured that G3BP1 disrupts the interaction of USP10 with p53 by binding to USP10, and consequently suppresses USP10 mediated deubiquitination of p53."
24998844
reach
"Resveratrol enhances USP10 mediated deubiquitination of p53 by disrupting the G3BP1 and USP10 interaction."
24998844
reach
"We therefore hypothesized that resveratrol interferes with the G3BP1 and USP10 interaction."
24998844
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"As expected, the interaction between G3BP1 and USP10 decreased in HCT116 cells treated with resveratrol (XREF_FIG)."
24998844
reach
"Resveratrol promotes the p53-mediated apoptosis of cancer cells by acting on the USP10-binding protein G3BP1 [36]."
35627217
reach
"An in vitro competition assay also showed that resveratrol inhibited the G3BP1 and USP10 interaction in a dose dependent manner (XREF_FIG)."
24998844
reach
"These results strongly indicate that resveratrol enhances USP10 mediated deubiquitination of p53 by disrupting the G3BP1 and USP10 interaction."
24998844
reach
"Overall, our study demonstrates that resveratrol directly targets G3BP1, which in turn prevents the G3BP1 and USP10 interaction and consequently increases USP10 regulated deubiquitination of p53 (XREF_FIG)."
24998844
reach
"G3BP1 binds to USP10 and inhibits the deubiquitination activity of USP10."
24998844
reach
"As a mechanism of resveratrol induced p53 expression, we revealed that resveratrol directly binds to G3BP1 and disrupts the G3BP1 and USP10 interaction, releasing USP10 which in turn results in enhanced USP10 mediated deubiquitination of p53."
24998844
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"By binding to both G3BP and PABP (a translational enhancer), USP10 could simultaneously retain mRNPs in a soluble state and prime them for translational activation and consequent removal from SGs."
27022092
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"Referring to three published studies, we noticed that USP10 could also bind with TP53, BECN1 and G3BP in other cancer cells including breast cancer, cervical cancer, colon cancer and osteosarcoma [ 31[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34914965
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"In addition, several viruses (Semliki Forest, Chikungunya, and herpes simplex) encode SG inhibiting proteins that share the same binding motif (FGDF motif) within USP10 that binds to G3BP."
30082464
sparser
"To test whether G3I compounds have the same effect as nsP3 peptide, we added these compounds to lysates from U2OS cells expressing G3BP1-GFP and used coimmunoprecipitation to assess the interaction between G3BP1 and endogenous caprin 1 and USP10."
38284934
sparser
"As predicted, the addition of G3Ib to these lysates resulted in a dose-dependent reduction of the interaction between G3BP1 and both endogenous caprin 1 and endogenous USP10, whereas the inactive enantiomer G3Ib′ had no such effect ( xref )."
38284934
sparser
"The interactions between USP10 and G3BP1 family proteins were first described in a global proteomic analysis of deubiquitinates and their associated protein complexes xref , xref ."
39863576
reach
"Cif stabilizes USP10 and G3BP1 interaction to inhibit USP10 activity."
21455491
reach
"Three observations suggest that Cif may inhibit USP10 activity by stabilizing the interaction between USP10 and G3BP1, which inhibits USP10 DUB activity, and also reduces the interaction between USP10 and CFTR."
21455491
reach
"To test whether G3I compounds had the same effect as nsP3 peptide, we added these compounds to lysates from U2OS cells expressing G3BP1-GFP and used co-immunoprecipitation to assess the interaction between G3BP1 and endogenous caprin1 and USP10."
37425931
reach
"Second, in U2OS bladder cancer cells, the interaction between G3BP1 and USP10 inactivates the deubiquitinating enzyme activity of USP10 XREF_BIBR."
21455491
reach
"Thus, studies were conducted to determine if Cif inhibits USP10 activity by stabilizing the interaction between USP10 and G3BP1, and by reducing the interaction between USP10 and CFTR."
21455491
reach
"Moreover, Cif increased the interaction between G3BP1 and USP10 by 210 +/-12% (XREF_FIG)."
21455491
reach
"Thus, these observations reveal that Cif stabilizes an interaction between USP10 and G3BP1, and also reduces the interaction between USP10 and CFTR."
21455491
reach
"Indeed, we have demonstrated that G3Ia and G3Ib inhibit the interaction of G3BP1 with caprin 1 and USP10 (Figure S1)."
37425931
reach
"To provide additional support for the immunoprecipitation studies demonstrating that Cif increases the interaction between G3BP1 and USP10 in the early endosomal compartment, we performed bimolecular fluorescence complementation (BiFC) studies."
21455491
reach
"To provide additional support for our immunoprecipitation studies that the interaction between USP10 and G3BP1 occurs in early endosomes (XREF_FIG), BiFC experiments were performed in cells transduced with a baculovirus system expressing eRFP labeled Rab5a (an early endosomal protein) to label early endosomes."
21455491
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"Treatment of co-transfected (YN-USP10 and YC-G3BP1) airway epithelial cells with Cif containing OMV resulted in an increase in BiFC signal, confirming an increased (4.35 +/-0.30 fold) interaction between USP10 and G3BP1 in the presence of Cif (XREF_FIG) compared to cells treated with control (Cif mutant OMV)."
21455491
reach
"These studies support the conclusion that Cif stabilizes the interaction between USP10 and G3BP1 in the early endosomes."
21455491
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"Finally, if Cif stabilizes an inhibitory interaction between G3BP1 and USP10, silencing G3BP1 should reduce the Cif induced decrease in USP10 activity as well as the Cif induced increase in ubiquitinated CFTR, and its degradation in lysosomes."
21455491
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"Caprin1 binding to G3BP promotes SG formation, whereas USP10 binding to G3BP inhibits SG assembly ( Kedersha et al., 2016 )."
27693920
sparser
"G3BP interacts with the deubiquitinating protease USP10 and plays an important role in the assembly of SGs."
22328580
sparser
"The DUB USP10 binds G3BP, localizes to SGs and regulates SG formation, even though the exact role of its DUB activity in this process remains controversial ( xref ; xref ; xref )."
33687997