IndraLab

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"To test whether there is an Erk independent function of Mek, RNA sequencing (RNA-seq) is carried out on six samples, WT KH2 ESCs treated with or without PD0325901 (PD) for 48h (KH2_PD and KH2, respectively), iErk1; Erk KO ESCs cultured in the presence of Dox (P0), 48 and 96h after Dox withdrawal (P1 and P2, respectively), and iErk1; Erk KO ESCs cultured without Dox for 96h, and treated with PD in the last 48h (P2_PD)."