IndraLab

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1a-4 increases the amount of HCN1. 6 / 6
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"To test the hypothesis that TRIP8b (1a-4) enhances HCN1 surface expression and targets the channel to its proper dendritic locale whereas TRIP8b (1a) prevents axonal expression of the channel, we examined the effects of viral overexpression of these two TRIP8b isoforms, both fused to an HA tag to allow us to distinguish exogenous from endogenous protein."
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"Of the two remaining hippocampal TRIP8b isoforms, TRIP8b (1a-4) promoted HCN1 surface expression in dendrites whereas TRIP8b (1a) suppressed HCN1 misexpression in axons."
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"In contrast, TRIP8b (1a-4) enhances surface expression of HCN1."
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"Specifically, TRIP8b (1a-4) increases HCN1 surface expression (Zolles et al., 2009), whereas TRIP8b (1b-2) inhibits the transport of HCN1 channel from the plasma membrane (Biel et al., 2009), thereby strongly downregulating HCN1 expression on the cell membrane."
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"TRIP8b (1a-4) and TRIP8b (1a-2-4) (the naming convention lists the alternatively spliced exons) cause a 4-6 fold increase in surface expression of HCN1 channels when co-expressed in Xenopus oocytes."
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"In contrast, TRIP8b (1a-4) increases the level of GFP-HCN1 associated with the dendritic plasma membrane when over-expressed in neurons in vivo (see below, Figure 7)."
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