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EGF phosphorylates RAF1. 35 / 35
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"In this report, we show that EGF phosphorylates and activates Raf-1 and B-Raf."
12644307
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"When overexpressed in cells, all variants were unable to negatively modulate EGF-promoted RAF1, MEK, and ERK phosphorylation, and time-course experiments in primary fibroblasts (p."
34626534
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"Both estrogen and epidermal growth factor (EGF) induced phosphorylation of c-raf and ERK1/2 while stimulating the proliferation of SKBR3 cells [XREF_BIBR], and the two agents were more effective in combination."
19308726
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"EGF stimulated the phosphorylation of ERK and Raf-1 in both ADPKD and HKC cells, but had no effect on B-Raf."
12753285
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"Moreover, the Ser338 phosphorylation of Raf-1 induced by epidermal growth factor (EGF) stimulation is impaired by an inhibitor of phosphoinositide 3-kinase (PI 3-kinase) 17 suggesting that PAK2 mediat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11316611
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"Epidermal growth factor stimulates phosphorylation of RAF-1 independently of receptor autophosphorylation and internalization."
1645717
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"Consistent with this, we found that MEK inhibition inhibits CRAF NtA phosphorylation induced by EGF."
23993095
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"The result showed that melatonin significantly increased the phosphorylation of c-Raf, ERK1/2 and c-Myc and EGF significantly increased phosphorylation of c-Raf, ERK1/2 and c-Myc when compared with control."
28275319
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"Defects in the EGFR signaling pathway cascade were found in all examined drug resistant sublines, including altered EGF-induced Shc, Raf-1, or mitogen-activated protein kinase phosphorylation."
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"Moreover, simultaneous knockdown of both PAK2 and PLA2G7 did not further attenuate EGF-stimulated S338-phosphorylation of RAF1 or phosphorylation of MEK and ERK (Figure 7F, right three lanes), which further support that PLA2G7 and PAK2 exist in the same signal pathway to regulate RAF1 activation."
35417664
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"Epidermal growth factor stimulates phosphorylation of RAF-1 independently of receptor autophosphorylation and internalization."
8381718
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"Depletion of prohibitin by siRNA blocked serum- or EGF-induced phosphorylation of the ERK and Raf1 signaling kinases, demonstrating a requirement of prohibitin in Ras-Raf1-MAPK signal transduction ( xref )."
21566741
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"Di Guglielmo, G. M., Baass, P. C., Ou, W. J., Posner, B. I., Bergeron, J. J. (1994) : Compartmentalization of SHC, GRB2 and mSOS, and hyperphosphorylation of Raf-1 by EGF but not insulin in liver pare[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11370743
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"Moreover, we found that the amplitude of basal and EGF-induced phosphorylation of RAF1 was elevated in Phlpp1 −/− MEF compared to WT cells, which led to increased activation of MEK and ERK downstream of RAF1 ( xref )."
24530606
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"Down-regulation of protein kinase C did not affect the EGF-induced increase in RAF-1 phosphorylation."
1645717
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"These experiments confirmed that EGF was able to induce signaling in all cell lines.To determine whether the increased EGFR expression was accompanied by activation of the signaling pathway cascade, w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10903426
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"EGF-induced RAF-1 serine phosphorylation required the intrinsic tyrosine kinase activity of the EGF receptor but was independent of EGF receptor self-phosphorylation and of ligand-induced receptor internalization."
1645717
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"Epidermal growth factor stimulates phosphorylation of RAF-1 independently of receptor autophosphorylation and internalization."
8381718
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"Thus phosphorylation of c-Raf in response to EGF does not 16 S. Traverse, P. CohenIFEBS Letters 350 (1994) 13-18 A- extract EGF 01 2 3 4 5 15 30 60 0 min -- 69 NGF 0 12 3 4 5 15 30 0 min B-Mono Q 1 2 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
8062910
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"When overexpressed in cells, all variants were unable to negatively modulate EGF-promoted RAF1, MEK, and ERK phosphorylation, and time-course experiments in primary fibroblasts (p.Leu100Pro and p.Leu381Hisfs ∗ 95) documented an increased and prolonged activation of the MAPK cascade in response to EGF stimulation."
34626534
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"Overexpression of either PHLPP isoform resulted in a significant decrease in both basal and EGF-induced phosphorylation of RAF1, and this decrease in RAF1 phosphorylation coincided with reduced activation of downstream MEK and ERK ( xref )."
24530606
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"To confirm that PHLPP-mediated effect depends on its ability to dephosphorylate RAF1, we showed that EGF-induced phosphorylation of RAF1 and subsequent activation of MEK and ERK were inhibited by PHLPP even in cells overexpressing KRAS G12V , whereas overexpression of RAF1/DDED rendered PHLPP incapable of inducing dephosphorylation of MEK and ERK."
24530606
sparser
"Di Guglielmo, G. M., Baass, P. C., Ou, W. J., Posner, B. I., Bergeron, J. J. (1994): Compartmentalization of SHC, GRB2 and mSOS, and hyperphosphorylation of Raf-1 by EGF but not insulin in liver paren[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11370743
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"We have confirmed that EGF activation of the classical receptor-tyrosine-kinase pathway leads to strong phosphorylation of Raf-1 and ERK in both ADPKD and HKC cells Figure 8."
12753285
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"Both estrogen and epidermal growth factor (EGF) induced phosphorylation of c-raf and ERK1/2 while stimulating the proliferation of SKBR3 cells [ xref ], and the two agents were more effective in combination."
19308726
sparser
"Consistent with this, we found that MEK inhibition inhibits CRAF NtA phosphorylation induced by EGF."
23993095
reach
"Compartmentalization of SHC, GRB2 and mSOS, and hyperphosphorylation of Raf-1 by EGF but not insulin in liver parenchyma."
7925272
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"Activation ofRas by growth factors such as epidermal growth factor (EGF) phosphorylates C-Raf, causing its translocation from cytosol to the plasma membrane."
19815182
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"Inhibition of Pak reduced the Ser (338) phosphorylation of c-Raf in response to both PDGF and EGF; however, in the case of EGF, the reduction in Ser (338) phosphorylation was not accompanied by a significant decrease in c-Raf activity."
16129686
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"In this report, we show that EGF phosphorylates and activates Raf-1 and B-Raf."
12644307
sparser
"EGF stimulated the phosphorylation of ERK and Raf-1 in both ADPKD and HKC cells, but had no effect on B-Raf."
12753285
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"Both TRH and EGF similarly increased the phosphorylation of raf-1, by a PKC-independent mechanism."
7508919
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"Immunoprecipitation analysis found that heparin inhibited serum, PMA, and PDGF but not EGF induced raf-1 phosphorylation."
9207927
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"Furthermore, although B-Raf and c-Raf are phosphorylated in PC 12 cells in response to NGF or EGF, phosphorylation does not lead to, or correlate with, activation."
8062910
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"In mammalian cells Raf-1 is rapidly hyperphosphorylated in response to PDGF, NGF, aFGF, EGF, insulin, CSF-1, steel factor and IL-2, all of which activate specific tyrosine kinases [13,16,28,121,141,18[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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