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PSMD14 inhibits E2F1. 4 / 5
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"Furthermore, we generated different siRNA resistant (siR-R) POH1 constructs for rescue assays, and the results showed that restoration of POH1 expression with the C120S-, H113Q- and DeltaJAMM POH1 mutants did not attenuate the inhibition of E2F1 caused by the deletion of endogenous POH1 (XREF_FIG)."
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"Briefly , PSMD14 suppressed the degradation of E2F1 , and then E2F1 promoted AKT / SOX2 positive feedback loop ."
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"Then , we validated that the ubiquitination level of E2F1 was greatly elevated in HNSCC cells stably transfected with shPSMD14 ( Figure 5E ) , indicating that PSMD14 prevented the degradation of E2F1 through ubiquitin-proteasome system ( UPS ) ."
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"Overexpression of the K63 resistant form of ubiquitin (K63R) attenuated POH1 knockdown induced E2F1 downregulation, indicating that K63 linked polyubiquitination is important for POH1 mediated E2F1 turnover (XREF_FIG)."
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