"The interactions of Siva1 with both p53 and Hdm2 are weakened by DNA-damage signaling, leading to the disruption of the p53-Siva1-Hdm2 complex."
"The precise mechanism of the Hdm2, Siva1, and p53 complex disruption remains to be determined."
"Siva1 interacts with both p53 and Hdm2, and facilitates Hdm2-mediated ubiquitination and degradation of p53."
"Together, these data suggest that DNA-damage signals disrupt the p53, Hdm2, and Siva1 complex through the activation of ATM."
"Consistent with this notion, Siva1 binds to both Hdm2 and p53 through non-overlapping regions, enhances Hdm2 interaction with p53, and promotes p53 degradation in an Hdm2-dependent manner."
"Consistently, DNA damage signaling weakened the Siva1, p53, and Hdm2 complex (XREF_FIG, lane 6 versus lane 5)."
"Upon DNA damage, the interactions of Siva1 with both p53 and Hdm2 are diminished."
"The observation that Siva1 interacts with p53 and Hdm2 through non-overlapping regions is consistent with the notion that Siva1 may promote the p53-Hdm2 interaction."
"XREF_BIBR To investigate how DNA damage regulates the p53, Siva1, and Hdm2 complex, ATM expression was knocked down by siRNA (XREF_FIG, lanes 7, 8 versus lanes 5, 6)."
"To test whether Siva1Δ C affects the ability of Siva1 to enhance p53-Hdm2 interaction, we tested the Hdm2-p53 interaction in the presence of Siva1 alone or Siva1 plus Siva1ΔC. As expected, the Hdm2-p53 interaction was enhanced when Siva1 was present ( xref , lane 5 versus lane 4)."
"Furthermore, Siva1 mutants that can bind to either p53 or Hdm2, but not both, have no effect on the stability of p53 ( xref )."
"The disruption of Siva1-p53 and Siva1-Hdm2 interactions occurred when cells were treated with MG132, which blocked Mdm2 and p53 degradation and equalized the levels of these two proteins under unstressed and stressed conditions."
"On DNA damage, the interactions of Siva1 with both p53 and Hdm2 are diminished."
"XREF_BIBR, XREF_BIBR The interactions of Siva1 with both p53 and Hdm2 are weakened by DNA-damage signaling, leading to the disruption of the p53, Siva1, and Hdm2 complex."
"Siva1 binds to both p53 and Hdm2 through distinct regions and enhances Hdm2-mediated p53 ubiquitination and degradation."
"Siva1-p53 and Siva1-Hdm2 interactions are regulated by DNA damage signaling."
"The short isoform of Siva1, Siva2, forms a ternary complex with p53 and Mdm2 but fails to destabilize p53."