IndraLab

Statements

TP53 binds SIVA1 and MDM2. 17 / 17
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"The precise mechanism of the Hdm2, Siva1, and p53 complex disruption remains to be determined."
19590512
reach
"Together, these data suggest that DNA-damage signals disrupt the p53, Hdm2, and Siva1 complex through the activation of ATM."
19590512
sparser
"Siva1-p53 and Siva1-Hdm2 interactions are regulated by DNA damage signaling."
19590512
sparser
"Siva1 interacts with both p53 and Hdm2, and facilitates Hdm2-mediated ubiquitination and degradation of p53."
19590512
reach
"Consistently, DNA damage signaling weakened the Siva1, p53, and Hdm2 complex (XREF_FIG, lane 6 versus lane 5)."
19590512
sparser
"The observation that Siva1 interacts with p53 and Hdm2 through non-overlapping regions is consistent with the notion that Siva1 may promote the p53-Hdm2 interaction."
19590512
reach
"XREF_BIBR To investigate how DNA damage regulates the p53, Siva1, and Hdm2 complex, ATM expression was knocked down by siRNA (XREF_FIG, lanes 7, 8 versus lanes 5, 6)."
19590512
sparser
"Furthermore, Siva1 mutants that can bind to either p53 or Hdm2, but not both, have no effect on the stability of p53 ( xref )."
19590512
sparser
"The short isoform of Siva1, Siva2, forms a ternary complex with p53 and Mdm2 but fails to destabilize p53."
19590512
sparser
"Consistent with this notion, Siva1 binds to both Hdm2 and p53 through non-overlapping regions, enhances Hdm2 interaction with p53, and promotes p53 degradation in an Hdm2-dependent manner."
19590512
sparser
"Upon DNA damage, the interactions of Siva1 with both p53 and Hdm2 are diminished."
19590512
sparser
"The interactions of Siva1 with both p53 and Hdm2 are weakened by DNA-damage signaling, leading to the disruption of the p53-Siva1-Hdm2 complex."
19590512
sparser
"To test whether Siva1Δ C affects the ability of Siva1 to enhance p53-Hdm2 interaction, we tested the Hdm2-p53 interaction in the presence of Siva1 alone or Siva1 plus Siva1ΔC. As expected, the Hdm2-p53 interaction was enhanced when Siva1 was present ( xref , lane 5 versus lane 4)."
19590512
sparser
"The disruption of Siva1-p53 and Siva1-Hdm2 interactions occurred when cells were treated with MG132, which blocked Mdm2 and p53 degradation and equalized the levels of these two proteins under unstressed and stressed conditions."
19590512
sparser
"On DNA damage, the interactions of Siva1 with both p53 and Hdm2 are diminished."
19590512
reach
"XREF_BIBR, XREF_BIBR The interactions of Siva1 with both p53 and Hdm2 are weakened by DNA-damage signaling, leading to the disruption of the p53, Siva1, and Hdm2 complex."
19590512
sparser
"Siva1 binds to both p53 and Hdm2 through distinct regions and enhances Hdm2-mediated p53 ubiquitination and degradation."
19590512