IndraLab

Statements


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"For the AMSH treatment, substrates were modified with 400 muM wild-type ubiquitin, then treated with 20 muM AMSH for 30 minutes at RT before analysis by SDS-PAGE and visualization by 5-FAM fluorescence on a ChemiDoc (Bio-Rad)."

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"71 In contrast to PAR2, USP8 (or AMSH) does not impact CXCR4 ubiquitination but instead modulates the ubiquitin status of ESCRT-0 that is ubiquitinated by the E3 ligase AIP4, 23 reinforcing the idea that ubiquitination of the transport machinery represents an important regulatory event in GPCR trafficking."

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"Similarly, endosomal deubiquitinase AMSH (Associated Molecule with the SH3-domain of STAM) has been shown to antagonize the ubiquitin dependent lysosomal degradation of EGFR by trimming the K63 linked ubiquitin chains [XREF_BIBR] while other studies have shown this DUB to be required for MVB dependent EGFR degradation [XREF_BIBR, XREF_BIBR]."