IndraLab

Statements

AR is glycosylated. 36 / 36
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"The role of glycosylation in α 2a -AR trafficking was previous examined in polarized MDCK cells, where a glycosylation deficient mutant α 2a -AR displayed normal binding and trafficking to the basolateral membrane ( xref )."
20059747
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"β 1 AR glycosylation influences responses to agonistic autoantibodies."
28801655
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"These data reveal that blocking β 1 AR N-linked glycosylation impairs β 1 AR dimerization."
12270132
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"The observation that the untagged β 1 AR is processed (like the tagged β 1 AR) to a ~69-kDa protein that contains sialylated N- and O-linked glycans in ldlD cells grown with Gal/GalNAc establishes that the N- and C-terminal tags do not influence β 1 AR glycosylation."
28801655
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"Taken together, the immunofluorescence, FACS analysis, and endoglycosidase experiments suggest that asparagine-linked glycosylation of α 2c -ARs is not the predominate mechanism responsible for the distinct trafficking behavior observed for the α 2c -ARs."
20059747
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"N-Linked glycosylation of the alpha(1B)-AR occurs at four asparagines on the N-terminus of the receptor."
11914074
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"MS/MS analysis of β 2 AR following PNGase F treatment lead to the identification of peptides originating from the N-terminus (residues 1-19), confirming that β 2 AR was heavily glycosylated."
20058880
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"The average median fluorescent intensity of cell surface expressed receptors obtained for three α 2c -AR glycosylation mutants, α 2c - (N19Q)-AR, α 2c - (N33Q)-AR and α 2c - (N19Q/N33Q)-AR, were lower than wild type α 2c -AR (57+/− 11%, 54 +/− 12% and 46.5 +/− 5%, respectively)."
20059747
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"Here we demonstrate that the polypeptide GalNAc-transferase 2 (GalNAc-T2) specifically O -glycosylates β 1 AR at five residues in the extracellular N terminus, including the Ser-49 residue at the location of the common S49G single-nucleotide polymorphism."
28167537
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"β 1 AR Glycosylation Sites."
28801655
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"However, the 4-epimerase defect can be bypassed and oligosaccharide synthesis can be fully restored by the addition of Gal and GalNAc to the culture medium, making ldlD cells a unique resource to map β 1 AR glycosylation sites."
28801655
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"The mechanism study suggested that the key pathway of ortho -C Ar -H glycosylation might undergo oxidative addition/reduction elimination, while aryl meta -C-H glycosylation was mediated by σ -activation strategy."
35674418
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"Therefore, we mapped β 1 AR glycosylation sites and determined whether β 1 ARs glycosylation prevents β 1 AR cleavage."
28801655
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"The selective biotinylation of the mature glycosylated α2 AR at the plasma membrane served as a positive control for our biotinylation conditions."
24098485
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"We found that the alpha(1B)-AR undergoes N-linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation."
11914074
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"We examined the potential glycosylation of Flag-tagged β 1 AR heterologously expressed in HEK-293 cells, which do not express endogenous β 1 AR."
12270132
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"Our data indicate, however, that the action of Con A on blocking β 1 AR internalization is a direct effect mediated via association of Con A with the carbohydrate portion of the receptor, since the N1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12270132
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"β 1 AR glycosylation patterns were interrogated in Chinese hamster ovary (CHO) ldlD cells, a cell line that is UDP-galactose/UDP-N-acetylgalactosamine 4-epimerase-deficient."
28801655
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"To determine if Asn15 is the only site of β 1 AR N-linked glycosylation, we performed enzymatic deglycosylation studies."
12270132
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"It is likely that the glycosylation of α 2c -ARs improves overall receptor biosynthesis and trafficking to the PM by increasing the efficiency of protein folding."
20059747
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"We show here that the beta(1)AR is glycosylated in various cell types and that mutation of the single predicted site of N-linked glycosylation (N15A) results in the formation of receptors that are not N-glycosylated."
12270132
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"Therefore, while glycosylation of the beta AR does not appear to be essential for receptor function, it is important for correct trafficking of the beta AR protein through the cell."
2162359
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"The small amount of REEP1/2/6 co-localization with α2C ARs ( xref ), most likely represents the site of interaction of the minimally glycosylated form, which is a minor contributor to the total pool of mature and immature glycosylated α2C ARs, possibly accounting for the low amount of co-localization seen."
24098485
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"Our findings indicate that the Semliki Forest virus system can provide large amounts of functional and fully glycosylated alpha(1B)-AR protein suitable for biochemical and structural studies."
11914074
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"To further study the effect of Rab43 on the ER-Golgi transport of GPCRs, we measured the effect of Rab43 on the conversion from simple to complex N-linked glycosylation of α 2A -AR and AT1R."
29069590
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"Since the β 2 AR N-terminus is glycosylated due to the presence of two asparagine-dependent glycosylation sites xref , we examined whether these N-glycan chains might be important for receptor activation by N. meningitidis ."
31628314
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"To elucidate the potential role of glycosylation in α 2c -AR trafficking and processing, three asparagine-linked glycosylation deficient α 2c -AR constructs were created: α 2c - (N19Q)-AR, α 2c - (N33Q)-AR and α 2c -(N19Q/N33Q)-AR."
20059747
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"However, the topology of around half of β 1 AR-TMD1 molecules is inverted in ΔEMC6 microsomes, explaining the impaired N-terminal glycosylation of various β 1 AR constructs ( xref A)."
30415835
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"Studies with Ser 49 - and Gly 49 -β 1 AR variants yielded similar results, indicating that β 1 AR glycosylation patterns are not grossly influenced by the S49G polymorphism."
28801655
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"Based on the homology with other seven transmembrane-helix GPCRs, xref , xref , xref glycosylation and phosphorylation of the N-terminus of β 2 AR was expected."
20058880
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"We recently identified a similar immunoreactive profile for this transgene in 2 different model cell lines and showed that the ∼69-kDa species corresponds to FL fully glycosylated β 1 ARs; the ∼55-kDa species corresponds to N-terminally truncated glycosylation-defective β 1 ARs xref ."
30175276
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"In addition, the glycosylation of α 2c -ARs was not required for appropriate folding to a functional confirmation, since each of the glycosylation deficient α 2c -AR constructs were determined to be functional by radioligand binding."
20059747
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"It is interesting to speculate that developmental- or disease-associated changes in the relative abundance of ppGalNAcT family enzymes that initiate O-glycosylation), the repertoire of glycosyltransferase enzymes that extend the core O-glycan structure (that produce diverse ensembles of branched glycan structures), or the expression of sialyltransferase enzymes that cap O- and N- linked glycans with sialic acid might impact on β 1 AR glycosylation (and secondarily β 1 AR cleavage and β 1 AR responsiveness)."
28801655
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"The aforementioned post-translational glycosylation of ARs, which may vary in a cell type-dependent fashion, likely accounts for these discrepancies."
10722120
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"Phosphorylation or glycosylation of the AR complex may result in activation by way of inhibition or prevention of rebinding of the chaperones."
24759590
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"This finding indicates that glycosylation of α 2c -AR improves overall express but does not dictate the intracellular retention of this receptor."
20059747