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USP30 binds AS1. 336 / 336
| 336
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"USP30-AS1 could suppress cell proliferation and metastasis of colon cancer via sponging miR-765."
35260141
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"Based on six signature lncRNAs (LINC00899, LINC01503, PRKAG2-AS1, RAD21-AS1, SRRM2-AS1 and USP30-AS1), the risk score (RS) system was constructed."
| PMC
sparser
"To the best of our knowledge, the presented reported for the first time that PTP4A1 could be regulated by USP30-AS1 at the post-transcriptional level in cervical cancer."
33986866
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"Utilizing The Cancer Genome Atlas (TCGA) database, it was revealed that USP30-AS1 was one of the most dysregulated lncRNAs in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), suggesting that USP30-AS1 may exert important roles during cervical cancer progression."
33986866
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"On the other hand, lncRNAs as NRIR, MIR155HG, U62317.2, USP30-AS1 and TNK2-AS1 contain a reduced number of RBPbinding sites (<2 CLIP sites per 1000 nucleotides), thus are likely involved in the activity of lncRNA-centered regulatory complexes[51,53]."
| DOI
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"Thus, we conducted a stepwise Cox regression analysis of 37 lncRNAs with survival significance and finally obtained a gene combination ( xref , xref ) composed of seven lncRNAs (HLA-DQB1 antisense RNA 1 (HLA-DQB1-AS1), USP30 antisense RNA 1 (USP30-AS1), AL645929, AL365361, long intergenic nonprotein coding RNA 520 (LINC00520), LINC00324, and AC055822)."
34250091
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"The prognostic value of USP30-AS1 in colon cancer."
35260141
sparser
"As the present study demonstrated that USP30-AS1 functions as a ceRNA to positively regulate PTP4A1 expression by sequestering miR-299-3p in cervical cancer, rescue experiments were performed to understand whether the tumour-promoting roles of USP30-AS1 were mediated by the miR-299-3p/PTP4A1 axis."
33986866
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"While the elevated expression of miR-765 could attenuate the inhibitory effect of USP30-AS1 on the proliferation of colon cancer cells ( P < 0.01, Fig. xref C)."
35260141
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"The expression levels of all im-lncRNAs were significantly higher in IH than IL samples (USP30-AS1 P = 1.12e-18 , HCP5 P = 8.07e-15 , PSMB8-AS1 P = 1.15e-15 , AL133264.2 P = 4.07e-10 , LINC01684 P = 1.50e-11 , and LINC01506 P = 3.00e-10 , xref )."
33584801
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"It can be seen that the high expression of USP30-AS1 can inhibit AML cell apoptosis and promote the viability of AML cells."
34694569
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"Among the viral-induced lncRNAs, we selected a specific signature composed by six elements (NRIR, BISPR, MIR155HG, USP30-AS1, FMR1-IT1, and U62317.2), for functional association studies [44] ."
| DOI
sparser
"The lncRNAs components of the non-coding transcriptional signature induced in nasopharyngeal samples during SARS-CoV-2 infection, NRIR, LINC02068, BISPR, USP30-AS1, MIR155HG and AL512306.2, are significantly upregulated (Fig. 3)."
| DOI
sparser
"After knockdown of ASH2L, the cis -regulation of USP30-AS1 in USP30 and ANKRD13A expression was impaired."
34694569
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"Transfection with an overexpression vector can promote the expression of USP30-AS1 in HL-60 cells (*** p  < 0.001, Fig.  xref B)."
34694569
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"Thirteen prognostic FRlncRNAs (LINC00942, MAFG-DT, AL049840.3, AL136084.3, OCIAD1-AS1, AC062017.1, AC008074.2, AC018653.3, AL031775.1, USP30-AS1, LINC01767, AC132807.2, and AL354919.2) were identified to be significantly different, constituting an FRlncRNAs signature."
34484338
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"Further clinical study is needed to determine whether USP30-AS1 expression and the methylation levels at Cg03124318 locus are desirable biomarkers to assess the progression of AML."
34694569
sparser
"A marked increase in USP30-AS1 expression was identified in CESC tissues when compared with normal tissues ( xref )."
33986866
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"The overexpression of ASH2L increased USP30 and ANKRD13A expression (*** p  < 0.001) and attenuated the reduction USP30 and ANKRD13A after USP30-AS1 knockdown."
34694569
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"In vitro and in vivo experiments revealed that USP30-AS1 interference promoted cell apoptosis; restrained cell proliferation, migration and invasion in vitro , and hindered tumour growth in vivo ."
33986866
sparser
"Notably, a significant negative correlation was found between the expression levels of USP30-AS1 and miR-765 with a correlation coefficient of −0.776 ( P < 0.001, Fig. xref C)."
35260141
sparser
"While the binding sites between USP30-AS1 and miR-765 were predicted, and the inhibitory effect of miR-765 on the luciferase activity of wild-type USP30-AS1 was disclosed, which was enhanced by the knockdown of miR-765 ( P < 0.001, Fig. xref B)."
35260141
sparser
"ASH2L is implicated in the cis regulation of USP30-AS1 in USP30 and ANKRD13A expression."
34694569
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"However, the lack of USP30-AS1 expression directly affects the enrichment of USP30 and ANKRD13A promoters in H3K4me3 protein."
34694569
sparser
"These results suggested the significance of USP30-AS1 in the progression and prognosis of colon cancer."
35260141
sparser
"In summary, this study confirmed the cancer-promoting effect of USP30-AS1 in AML for the first time."
34694569
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"Five immune-related lncRNAs (H1FX-AS1, AL441992.1, USP30-AS1, AP001527.2, and AL031123.2) were selected for the construction of the prognostic model."
33328990
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"In colon cancer tissues, the USP30-AS1 downregulation and the miR-765 upregulation were observed, and there was a negative correlation between the USP30-AS1 expression level and the miR-765 expression level."
35260141
sparser
"To detect the impact of the high expression of USP30-AS1 on AML cells, we tested 5 cell lines that related to AML (including HL-60, KG-1, OCI-AML3, Kasumi-1, and THP1)."
34694569
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"In HeLa and CaSki cells, USP30-AS1 loss suppressed cell proliferation ( xref ) and increased cell apoptosis ( xref ), whereas PTP4A1 overexpression abolished these effects."
33986866
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"And we used the random forest method to find USP30-AS1 as a key mediating factor in SKCM."
36624362
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"Our data indicated that USP30 and ANKRD13A are regulated by USP30-AS1 through affecting histone methylation and acetylation."
34694569
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"Additionally, the detailed role of USP30-AS1 in cervical cancer remains unknown."
33986866
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"Calculate the risk score of ccRCC patients according to the formula: risk score = (0.217 × CARS expression level) + (0.0098 × CD44 expression level) + (−0.0053 × DPP4 expression level) + (−0.307 × USP30-AS1 expression level) + (0.103 × GCLC expression level) + (−0.151 × HMGCR expression level) + (−0.0006 × HSPB1 expression level) + (−0.0094 × NCOA4 expression level) + (−0.0024 × SAT1 expression level) + (0.1523 × PHKG2 expression level) + (−0.007 × GOT1 expression level) + (−0.0024 × HMOX1 expression level)."
33996565
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"Therefore, this study first confirmed the cancer-promoting effect of USP30-AS1 in AML, which may be an important lncRNA target in AML treatment."
34694569
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"Among these, LncRNA USP30−AS1 had co-expression relationship with 9 ferroptosis-related genes (CYBB, SOCS1, IFNG, TNFAIP3, PML, GCH1, NCF2, SLC2A6, and CAPG), LncRNA LIPE-AS1 was co-expressed with 5 ferroptosis-related genes (HRAS, PHKG2, MAPK14, EGLN2, and GPX4) and there was a co-expression relationship between AC108474.1 and 5 ferroptosis-related genes (TAZ, ISCU, CAV1, PLIN4, and HIC1)."
34164433
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"USP30-AS1 is an antisense lncRNA that locates on chr12 (q24,11), the opposite strand of USP30 locus."
35260141
sparser
"In conclusion, the miR-299-3p/PTP4A1 axis is the downstream effector of USP30-AS1 in cervical cancer, forming the USP30-AS1/miR-299-3p/PTP4A1 pathway."
33986866
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"All these results offer a new theoretical and experimental basis for developing USP30-AS1 as a diagnostic and prognostic biomarker as well as a therapeutic target for cervical cancer."
33986866
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"By comparing the noncoding RNAs of different immune‐infiltrating groups, we found that 6 differentially expressed lncRNAs (AC092580.4, CTA‐384D8.35, AC002331.1, USP30‐AS1, GATA3‐AS1, and AC019117.1) and 11 differentially expressed miRNAs (mir142, mir223, mir7702, mir4772, mir155, mir150, mir187, mir429, mir200a, mir551b, and mir200b) were associated with immune infiltration."
32096345
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"The previous tests confirmed that USP30-AS1 positively regulates the expression of USP30 and ANKRD13A. We noticed that the locations of the USP30 and ANKRD13A genes in the twelfth chromosome are very close to the location of the USP30-AS1 gene (Fig.  xref A)."
34694569
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"In addition, RAD21-AS1 [ xref ], SRRM2-AS1 [ xref ] and USP30-AS1 [ xref , xref ] have been reported to be survival prognosis in various cancers."
| PMC
sparser
"USP30-AS1 knockdown together with ANKRD13A overexpression, or individual overexpression of ANKRD13A and USP30-AS1 had no effect on HLA-1 protein level in KG-1 cells."
34694569
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"USP30-AS1 expression was measured via reverse transcription-quantitative PCR."
33986866
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"Next, we studied each lncRNA in our signature and found that AC024060.1, AC105942.1, AL031775.1, USP30-AS1, and MAFG-DT-LNC were merely reported once in BC predictive signatures, like immune-related lncRNA signature [ xref ], epithelial mesenchymal transition-related lncRNA signature [ xref ], tumor-infiltrating B lymphocytes lncRNA signature [ xref ]."
34281486
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"Among them, USP30 and ANKRD13A genes are close to USP30-AS1 gene in chromosome."
34694569
sparser
"The effect of USP30-AS1 on the cellular processes of colon cancer and its mechanism."
35260141
sparser
"Based on the approach, we identified 6 im-lncRNAs (USP30-AS1, HCP5, PSMB8-AS1, AL133264.2, LINC01684, LINC01506)."
33584801
sparser
"Similarly, USP30-AS1 is associated with prognosis in bladder cancer ( xref )."
34136413
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"Furthermore, in accordance with our findings, some other studies mainly based on the bioinformatics analysis have also reported that LINC02408 [ xref ] and YTHDF3-AS1 [ xref ] are connected with the poor prognosis of BC patients, but USP30-AS1 [ xref ], U73166.1 [ xref ], and LINC01016 [ xref ] can be taken as the protective factors of prognosis."
35205391
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"Knockdown of USP30, but not ANKRD13A, abolished the cancer-promoting effects of USP30-AS1."
34694569
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"We identified and included 15 prognostic ferroptosis-related lncRNAs (AL356740.1, FOXC2AS1, ZNF528AS1, LINC02535, PSMB8AS1, AL590428.1, AP000347.2, OCIAD1-AS1, AP001347.1, AC104986.2, AC018926.2, LINC00867, AC099518.4, USP30-AS1, and ARHGAP5-AS1), to build our ferroptosis-related lncRNAs risk model."
36595859
sparser
"Besides, in our study, HLA-DQB1-AS1, USP30-AS1, AL645929, AL365361, and AC055822 were also protective factors in patients at low risk."
34250091
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"Additionally, the expression analyses revealed that USP30-AS1 was downregulated ( xref ) but miR-299-3p was upregulated ( xref ) in the tumours in the USP30-AS1-silenced group."
33986866
sparser
"Patients with cervical cancer expressing high levels of USP30-AS1 exhibited shorter overall survival than those with low USP30-AS1 expression ."
33986866
sparser
"It is easy to think that the expressions of USP30 and ANKRD13A may be regulated by USP30-AS1 through the cis -action."
34694569
sparser
"However, results from bioinformatics analysis prompted us to further explore the regulatory effects of USP30-AS1 in AML cell lines."
34694569
sparser
"The result implies that PD-L1 was significantly associated with some FRlncRNAs, such as LINC02328, LINC01871, LMNTD2-AS1, LINC00702, LINC02384, AP001434.1, TRG-AS1, LIPE-AS1, HSD11B1-AS1, USP30-AS1, LINC02446, AC099329.2, PRKAR1B-AS1, and LINC02084."
34671639
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"Furthermore, we identified six immunophenotype-related lncRNA signatures (im-lncRNAs, including USP30-AS1, HCP5, PSMB8-AS1, AL133264.2, LINC01684, and LINC01506) using the minimum redundancy maximum relevance (mRMR) feature selection method and the random forest model."
33584801
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"Univariate Cox regression analysis results showed that AP000695.1 (HR: 1.73, 95% CI: 1.05–2.85, p -value: 0.033) was a risk factor of ovarian cancer while LINC00996 (HR: 0.422, 95% CI: 0.235–0.758, p -value: 0.00388) and USP30-AS1 (HR: 0.794, 95% CI: 0.677–0.931, p -value: 0.00464) were protective factors of ovarian cancer ( xref )."
34671615
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"Based on six signature lncRNAs (LINC00899, LINC01503, PRKAG2-AS1, RAD21-AS1, SRRM2-AS1 and USP30-AS1), the risk score (RS) system was constructed."
34225739
sparser
"Similarly, a larger percentage of lower USP30-AS1 was also observed in the patients with lymph node metastasis (63.8 vs. 36.2%, Fig. xref B) and advanced TNM stage (65.9 vs ."
35260141
sparser
"USP30-AS1 contributes to mitochondrial quality control in glioblastoma cells."
34653676
sparser
"Therefore, we speculated that low methylation level at Cg03124318 locus leads to the high expression of USP30-AS1 in AML."
34694569
sparser
"USP30-AS1 increases the expression of USP30 and ANKRD13A through a cis mechanism."
34694569
sparser
"USP30-AS1 is the newly discovered lncRNA transcribed from the antisense strand of the USP30 gene."
34694569
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"Mechanistically, USP30-AS1 competed for microRNA-299-3p (miR-299-3p) in cervical cancer and lowered the regulatory actions of miR-299-3p on protein tyrosine phosphatase type IVA (PTP4A1), resulting in PTP4A1 overexpression."
33986866
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"Rapid progress in sequencing technology nowadays has bought noncoding RNA (ncRNA) to light, exceptionally long non‐coding RNAs (lncRNAs), which have critical roles in malignant transformation and progression. xref LncRNAs are participators in regulating gene expression through chromatin modification and the process of transcriptional and posttranscriptional. xref As more and more studies on lncRNA function have been revealed, lncRNAs can regulate biologic processes and serve as a prognostic biomarker in many solid tumors. xref , xref , xref , xref , xref Moreover, lncRNAs also regulate the hemopoiesis system. xref For example, HOTTIP is abnormally activated in AML, and HOTTIP loss leads to the inhibition of genes crucial for hematopoiesis and AML leukemogenesis. xref Knockdown of LncRNA ANRIL results in a drop in glucose uptake and blockage of AML cell maintenance. xref In addition, lncRNA USP30‐AS1 is highly expressed in AML and promotes the survival of acute myeloid leukemia cells by cis‐regulating USP30 and ANKRD13A. xref "
34904791
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"Ten chemokine-related lncRNAs (AC010719.1, BX293535.1, LINC01094, AC008770.3, MIR3142HG, AC147067.2, AC005332.4, AC091043.1, ACTA2-AS1, USP30-AS1) were identified in the risk model."
35096827
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"The other six are protective lncRNAs with univariate Cox HR < 1 (MIF-AS1, AC008735.2, AL357033.4, LINC00649, AC099343.2 and USP30-AS1), which indicated that high expression of the six lncRNAs might result in a better survival time."
32579540
sparser
"Next, we established an immune-related prognostic signature based on eight lncRNAs targeting immune-related genes (WNT5A-AS1, AL136084.3, MIF-AS1, AC008735.2, AL357033.4, LINC00649, AC099343.2 and USP30-AS1) in the training cohort."
32579540
sparser
"In colon cancer cells, including COLO320, SW480, RKO, and HCT116cells, the significant downregulation of USP30-AS1 was also observed relative to its expression in the normal CCD841 cell ( P <0.001, Fig. xref A)."
35260141
sparser
"Next, we identified six immunophenotype-related long non-coding RNA biomarkers (im-lncRNAs, USP30-AS1, HCP5, PSMB8-AS1, AL133264.2, LINC01684, and LINC01506) by employing a machine learning computational framework combining minimum redundancy maximum relevance algorithm (mRMR) and random forest model."
33584801
sparser
"Based on the gene expression data in the TCGA and GTEx projects (), we utilized the bioinformatics technology to study the expression of USP30-AS1 in AML."
34694569
sparser
"Also, the correlation between USP30-AS1 expression and clinicopathological features in CESC was analysed employing TCGA database."
33986866
sparser
"In short, USP30-AS1 functions as a ceRNA for miR-299-3p and thereby upregulates PTP4A1 in cervical cancer."
33986866
sparser
"As shown in Fig.  xref A1, among a variety of cancers, USP30-AS1 has the most up-regulated expression in DLBC (Lymphoid Neoplasm Diffuse Large B cell Lymphoma) and LAML (Acute Myeloid Leukemia) tissues."
34694569
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"Analysis of the TCGA cohort revealed a six aging-related lncRNA signature consisting of MCF2L-AS1, USP30-AS1, OTUD6B-AS1, MAPT-AS1, PRR34-AS1, and DLGAP1-AS1."
37110517
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"Therefore, USP30 mediated the cancer-promoting effects of USP30-AS1 in AML."
34694569
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"Consistently, their expression was also significantly changed following USP30-AS1 knockdown."
34694569
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"Compared with normal tissues, USP30-AS1 was significantly higher in AML tissues (* p  < 0.05, Fig.  xref A2)."
34694569
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"Herein, the upregulation of miR-765 in colon cancer tissues was observed, which showed a negative correlation with USP30-AS1."
35260141
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"As indicated by immunofluorescence test (Fig.  xref C), knockdown of ANKRD13A and USP30-AS1 increased the abundance of HLA-1 protein in the cell membrane of KG-1 cells; the effect exerted by USP30-AS1 knockdown was abolished by ANKRD13A overexpression; overexpression of ANKRD13A and USP30-AS1 individually was associated with very low level of HLA-1 protein in the cell membrane of KG-1 cells."
34694569
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"According to results from bioinformatics analysis, lncRNA USP30-AS1 is highly expressed in AML and both the high expression of USP30-AS1 and low methylation level at Cg03124318 locus of USP30-AS1 gene promoter are associated with poor prognosis of AML."
34694569
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"Here, we used bioinformatics technology to characterize the roles of USP30-AS1 in AML."
34694569
sparser
"High level of USP30-AS1 is correlated with poor survival in both primary and recurrent glioma patients."
34653676
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"Previously, USP30-AS1 has been reported to mediate the progression of various human diseases, such as glioblastoma, cervical cancer, and acute myeloid leukemia [ xref – xref ]."
35260141
sparser
"In the knockdown test of USP30-AS1, we transfected the CRISPR/Cas9-sgRNA vector into KG-1 cells, and found the significant reduction of USP30-AS1 expression (*** p  < 0.001, Fig.  xref B), indicating that USP30-AS1 was successfully knocked down."
34694569
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"According to the average expression level of USP30-AS1 in colon cancer tissues, the enrolled patients were grouped as the low USP30-AS1 group with 64 patients and the high USP30-AS1 group with 59 patients."
35260141
sparser
"AC026369.3 ( xref ), LINC01871 ( xref ; xref ; xref ), USP30-AS1 ( xref ; xref ), and AC093297.2 ( xref ) could become potential prognostic biomarkers for various malignant tumors in bioinformatics approaches."
35186949
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"USP30-AS1 is a newly discovered lncRNA transcribed from the antisense chain of the USP30 gene, which is a novel mitochondrial deubiquitinase involved in the regulation of p53 stability and a variety of pathophysiological processes [ xref ]."
35031063
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"USP30-AS1 directly binds to miR-299-3p and functions as a miR-299-3p sponge in cervical cancer."
33986866
sparser
"This result is line with the effect of USP30-AS1 on inducing H3K4me3 in the promoter of USP30 and ANKRD13A genes."
34694569
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"In the USP30-AS1 (OE) group of cells, the enrichment of the USP30 and ANKRD13A promoters in the H3K27Ac protein was significantly increased (* p  < 0.05, Fig.  xref C)."
34694569
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"Alike, the suppression by USP30-AS1 overexpression was also observed in the migration (Fig. xref D) and invasion (Fig. xref E) of SW480 and HCT116 cells, which was reversed by the overexpression of miR-765 ( P < 0.001)."
35260141
sparser
"Taken together, the results suggest that USP30-AS1 is an endogenous miR-299-3p sponge in cervical cancer."
33986866
sparser
"While miR-765 showed a negatively regulatory effect on the luciferase activity of USP30-AS1 and alleviated the tumor suppressed the effect of USP30-AS1."
35260141
sparser
"However, the specific dysregulation and the function of USP30-AS1 in colon cancer remains unknown, which might be a biomarker of the tumor progression and an indicator of clinical prognosis."
35260141
sparser
"Among these eight lncRNAs, WNT5A-AS1 and AL136084.3 are risk lncRNAs, while MIF-AS1, AC008735.2, AL357033.4, LINC00649, AC099343.2 and USP30-AS1 are protective lncRNAs."
32579540
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"In glioblastoma, USP30-AS1 could negatively regulate mitophagy, which might lead to the loss of mitochondrial homeostasis and regulate the tumor progression [ xref ]."
35260141
sparser
"Accordingly, a 6-lncRNA signature risk prediction model (LINC00899, LINC01503, PRKAG2-AS1, RAD21-AS1, SRRM2-AS1 and USP30-AS1) was produced."
| PMC
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"Finally, a 6-lncRNA signature (KRT7-AS, USP30-AS1, AC011445.1, AP005205.2, DNM3OS, and AC027348.1) was determined, which could classify EOC patients into high-risk and low-risk groups, with a significant difference in OS between the two groups ( P  < 0.001)."
35031063
sparser
"This study employed the LASSO regression method to establish an autophagy-related lncRNA signature containing AC084018.1, AC092171.2, AP000695.1, GAS6-AS1, LINC00174, LINC00893, LINC00996, MEIS1-AS3, MIR22HG, NEAT1, TEX26-AS1, U73169.1, UBE2Q1-AS1, and USP30-AS1 for ovarian cancer based on abnormally expressed autophagy-related lncRNA profiles."
34671615
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"And USP30-AS1 is also a protective factor in the bladder [ xref ]."
34250091
sparser
"However, the detailed role of USP30-AS1 in cervical cancer remains to be elucidated."
33986866
sparser
"miR-765 was predicted as a potential target of USP30-AS1, and the function of miR-765 has been demonstrated in numerous human cancers."
35260141
sparser
"Due to ethical reason, we are currently unable to obtain enough bone marrow of AML patients and healthy subjects to identify the epigenetic changes of USP30-AS1, the expression of USP30-AS1 and their association with the prognosis of AML patients."
34694569
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"Forcing the expression of USP30-AS1 in HL-60 cells promoted cell viability while reducing the rate of cell apoptosis (* p  < 0.05, ** p  < 0.01, or *** p  < 0.001, Fig.  xref C and D)."
34694569
sparser
"Interestingly, NRIR, BISPR, USP30-AS1 and AL512306.2 lncRNAs were also detectable at higher levels in blood samples of SARS-CoV-2 patients."
| DOI
sparser
"Survival analysis showed that high expression of USP30-AS1 was associated with a lower overall survival rate (*** p  < 0.001, Fig.  xref B), indicating that the high expression of USP30-AS1 was associated with the poor prognosis of AML."
34694569
sparser
"The abnormal expression of USP30-AS1 has been widely reported, such as in cervical cancer, glioblastoma, ovarian cancer, bladder urothelial carcinoma, and cutaneous melanoma [ xref – xref ]."
35260141
sparser
"By correlation analysis and LASSO regression analysis, we constructed an immune-related lncRNA prognostic model based on five lncRNA: HLA-DQB1-AS1, MIR205HG, RP11-643G5.6, USP30-AS1 and RP11-415F23.4."
34675614
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"In mechanism, the interaction of USP30-AS1 with miR-765 was evaluated with the dual-luciferase reporter assay."
35260141
sparser
"In the SW480 and HCT116 cells, two cells sensitive to the downregulation of USP30-AS1, the proliferation of these two cells was found to be dramatically suppressed by the overexpression of USP30-AS1 ( P < 0.01, Fig. xref C)."
35260141
sparser
"However, USP30-AS1 does not regulate USP30 expression in a cis-regulatory manner."
34653676
sparser
"In addition, rescue experiments confirmed that miR-299-3p interventions or exogenous PTP4A1 counteracted the cancer-inhibiting actions of USP30-AS1 deficiency in cervical cancer cells."
33986866
sparser
"Therefore, sponging miR-765 was hypothesized as the molecular mechanism underlying the suppressor role of USP30-AS1 in colon cancer."
35260141
sparser
"At present, the regulatory role of USP30-AS1 in cancer has not yet in-depth studied, but its possible involvement in autophagy has mentioned in bladder cancer, which may act as a potential prognostic indicator [ xref ]."
34694569
sparser
"Hence, the regulation of target genes by miR-765 is also a potential molecular mechanism involved in the function of USP30-AS1 in colon cancer development."
35260141
sparser
"According to the results of the univariate Cox analysis, only the overexpression of LINC00520 was associated with poor overall survival, unlike HLA-DQB1-AS1, USP30-AS1, AL645929, AL365361, LINC00324, and AC055822."
34250091
sparser
"Furthermore, rescue experiments confirmed that miR-299-3p interventions or exogenous PTP4A1 could counteract the cancer-inhibiting actions of USP30-AS1 silencing on cervical cancer cells."
33986866
sparser
"In vitro and in vivo experiments revealed that USP30-AS1 downregulation induced cell apoptosis; suppressed cell proliferation, migration and invasion in vitro ; and impeded tumour growth in vivo ."
33986866
sparser
"Therefore, USP30-AS1 interference decreases the tumour growth of cervical cancer cells in vivo ."
33986866
sparser
"Nine optimal FRLs were filtrated and FRL CTC-246B18.8 was a risk factor with HR > 1, while FRLs AC133644.2, RP1-223E5.4, RP1-313I6.12, RP11-872J21.3, RP3-512B11.3, RP5-1120P11.1, SNHG10, and USP30-AS1 were protective factors with HR < 1 ( xref , xref )."
35127813
sparser
"Moreover, USP30-AS1 was also evidenced to act as an independent prognostic indicator of colon cancer that predicted a shorter survival time and poorer survival rate of patients, as well the TNM stage."
35260141
sparser
"Then, using bioinformatics analyses, the binding site between USP30-AS1 and miR-299-3p was determined, and subsequent mechanistic studies corroborated that USP30-AS1 functions as an endogenous sponge for miR-299-3p in cervical cancer."
33986866
sparser
"USP30-AS1 mediated repression of mitophagy may contribute to the loss of mitochondrial homeostasis and tumor development in glioma."
34653676
sparser
"The above results indicated that ten chemokine-related lncRNAs of the risk model (AC010719.1, BX293535.1, LINC01094, AC008770.3, MIR3142HG, AC147067.2, AC005332.4, AC091043.1, ACTA2-AS1, USP30-AS1) were associated with patients’ survival and immune landscape."
35096827
sparser
"Transwell migration and invasion assays revealed that the absence of USP30-AS1 hindered the migratory ( xref ) and invasive ( xref ) abilities of HeLa and CaSki cells."
33986866
sparser
"USP30-AS1, a type of ceRNA, competes for miR-299-3p in cervical cancer and thereby reverses the suppressive effects of miR-299-3p on PTP4A1, resulting in PTP4A1 overexpression."
33986866
sparser
"The results of the present study suggest that PTP4A1 is indirectly regulated by USP30-AS1 by decoying miR-299-3p."
33986866
sparser
"Furthermore, a RIP assay was performed to assess the potentially endogenous interaction among USP30-AS1, miR-299-3p and PTP4A1."
33986866
sparser
"USP30-AS1 suppressed the progression of colon cancer through modulating miR-765."
35260141
sparser
"Knockdown of USP30-AS1 decreases mitochondrial protein expression and mitochondrial mass, promotes mitochondrial uncoupler-induced mitophagy."
34653676
sparser
"Results of survival analysis showed that osteosarcoma with high expression of AC006033.2, LINC02315, AL133523.1, USP30-AS1, or AC079760.2 had a better prognosis ( xref and xref )."
34692688
sparser
"Combining with the fact that USP30-AS1 is highly expressed in KG-1 cells, we conjectured that USP30-AS1 can regulate these genes in AML, through cis or trans mechanism."
34694569
sparser
"The expression of USP30-AS1 and miR-765 in tissues samples."
35260141
sparser
"Through high-throughput sequencing studies, we found that USP30-AS1 regulates the expression of multiple genes in AML (including 324 up-regulated and 821 down-regulated genes)."
34694569
sparser
"A novel lncRNA, called USP30 antisense RNA 1 (USP30-AS1), has been studied in bladder urothelial carcinoma ( xref )."
33986866
sparser
"However, inhibiting the expression of USP30 in the overexpressed USP30-AS1 cells can inhibit the enhancement of cell viability and reduction of cell apoptosis."
34694569
sparser
"Among these 12 lncRNAs constructing the prognostic signature, LINC02408, AL589765.4, AL121790.2, YTHDF3-AS1, LINC00460, and CYTOR functioned as risk factors for the prognosis of BC patients, while AC068473.4, USP30-AS1, U73166.1, LINC00987, CD2BP2-DT, and LINC01016 acted as protective factors."
35205391
sparser
"Univariate and multivariate Cox regression analyses showed significant correlations between five autophagy-related lncRNAs, LINC02178, AC108449.2, Z83843.1, FAM13A-AS1 and USP30-AS1, and overall survival (OS) among BCLA patients."
32805727
sparser
"Genes regulated by USP30-AS1 are mainly related to genetic regulation and immune system."
34694569
sparser
"Among the five immune-related lncRNAs used to construct the model, H1FX-AS1, AL441992.1, USP30-AS1, and AL031123.2 were protective factors, while AP001527.2 was a risk factor."
33328990
sparser
"To address the effects of USP30-AS1 in cervical cancer, USP30-AS1-targeting siRNAs were synthesized to knock down endogenous USP30-AS1 expression, with si-NC as the control. xref depicts the efficiency of siRNAs in decreasing USP30-AS1 expression."
33986866
sparser
"USP30-AS1 is an lncRNA transcribed from the antisense chain of the USP30 gene."
34694569
sparser
"Nine arlncRNAs (USP30-AS1, LINC00665, PCED1B-AS1, LINC00324, LINC01871, ZEB1-AS1, LINC01527, AC018553.1, and HLA-DQB1-AS1) were identified to be related to the prognosis of melanoma patients."
35029926
sparser
"Among them, eight lncRNAs (AL031775.1, USP30-AS1, AC024060.1, AL162586.1, AP003352.1, PSMB8-AS1, AC016957.2, and STAG3L5P-PVRIG2P-PILRB) were deemed as protective predictors with hazard ratio < 1."
34281486
sparser
"The prognostic risk score formula of the signature was as follows: prognostic score = (0.2520 × KMT2E-AS1) + (-0.4604 × USP30-AS1) + (-0.5304 × ST7-AS1) + (-0.2505 × VPS9D1) + (-0.5438 × TFAP2A-AS1) + (0.2797 × OTUB6DB-AS1) + (- 0.1847 × HOXB-AS1) + (- 0.0873 ×LINC01087) + (- 0.4423×MAPT-AS1) ( P <0.05) ( xref )."
33614483
sparser
"The expression of USP30-AS1 was evaluated with PCR in tissue and cell samples."
35260141
sparser
"It has been reported that USP30-AS1 may be associated with cervical cancer, glioblastoma multiforme and bladder cancer, but this is mostly based on bioinformatics analysis [ xref , xref , xref ]."
35031063
sparser
"Through regulating USP30 and ANKRD13A, USP30-AS1 suppressed the apoptosis of acute myeloid leukemia cells, and therefore improved cell viability [ xref ]."
35260141
sparser
"To further determine that ASH2L is involved in the cis regulation of USP30-AS1, we conducted ChIP assay using H3K4me3 as the IP protein."
34694569
sparser
"USP30-AS1 interference markedly decreased PTP4A1 expression at both the mRNA ( xref ) or protein ( xref ) levels, whereas anti-miR-299-3p cotransfection reversed its regulatory actions on PTP4A1 expression ( xref )."
33986866
sparser
"In TrxG protein complex, ASH2L, in combination with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, SETD1A, and SETD1B. This study found that USP30-AS1 had strong affinity to ASH2L that belongs to TrxG protein complex."
34694569
sparser
"These results indicate that USP30 mediates the cancer-promoting effect of USP30-AS1 in AML cells."
34694569
sparser
"An optimal 8-lncRNAs (AC108474.1, AL133467.1, LINC01235, AC072039.2, TDRKH-AS1, USP30-AS1, MAPT-AS1, and LIPE-AS1) signature was identified based on the lowest Akaike information criterion (AIC) ( xref )."
34164433
sparser
"Furthermore, eight ferroptosis-related lncRNAs (AC072039.2, LINC01235, TDRKH−AS1, AC108474.1, AL133467.1, LIPE-AS1, MAPT-AS1, and USP30−AS1) were selected to construct a prognostic signature based on their performance in the multivariate Cox regression analysis."
34164433
sparser
"We further performed PCR and western blot studies to confirm the regulatory effect of USP30-AS1 on the five adjacent genes ( USP30 , ACACB , ANKRD13A , TMEM11 9, and SSH1 )."
34694569
sparser
"We compared the expression levels of six lncRNAs (KRT7-AS, USP30-AS1, AC011445.1, AP005205.2, DNM3OS and AC027348.1) in normal ovarian tissues and OC tissues (Fig.  xref A)."
35031063
sparser
"In collected tissues, a significantly reduced expression of USP30-AS1 was found in colon cancer tissues relative to the normal tissues ( P < 0.001, Fig. xref A)."
35260141
sparser
"At present, USP30-AS1 has been studied in bladder urothelial cancer and cervical cancer, which is related to autophagy, proliferation, and apoptosis [ xref , xref ]."
34694569
sparser
"However, fragments without the region (250–500 bp) showed very weak capacity to bind ASH2L. Therefore, the region (250–500 bp) is very important for USP30-AS1 binding to ASH2L. To determine whether ASH2L is implicated in the regulatory effect of USP30-AS1 on USP30 and ANKRD13A expression, we knocked down or overexpressed ASH2L in KG-1 and HL-60 cells (Fig.  xref D)."
34694569
sparser
"Then, 56 pairs of cervical cancer tissue and adjacent normal tissues were collected to determine USP30-AS1 expression."
33986866
sparser
"High-throughput sequencing was performed to explore the gene regulated by USP30-AS1."
34694569
sparser
"Xu et al. established a hierarchical cluster profile of aberrantly expressed lncRNAs in colon cancer with the data from the TCGA database and identified a number of potential functional lncRNAs, including lncRNA USP30-AS1 (USP30-AS1) [ xref ]."
35260141
sparser
"The results showed that six im-lncRNAs closely associated with the GBM immunophenotypes and four of the six (USP30-AS1, HCP5, AL133264.2, and LINC01506) were consistent with the im-lncRNAs identified in the TCGA GBM cohort ( xref )."
33584801
sparser
"Furthermore, miR-299-3p was expressed at low levels in cervical cancer tissues ( xref ) and exhibited an inverse expression correlation with USP30-AS1 ( xref )."
33986866
sparser
"Taken together, these observations unveil a new ceRNA network involving USP30-AS1, miR-299-3p and PTP4A1."
33986866
sparser
"USP30-AS1 can cis -regulate the USP30 gene to promote the progression of AML disease."
34694569
sparser
"Among these lncRNAs, AC091043.1, USP30-AS1, MIR3142HG, LINC01094 and ACTA2-AS1 were reported to regulate the progression of various tumors, while others were reported for the first time ( xref ; xref ; xref ; xref )."
35096827
sparser
"ChIP analysis revealed that the regulatory effects of USP30-AS1 on USP30 and ANKRD13A are associated with H3K4me3 and H3K27Ac."
34694569
sparser
"Apart from USP30 , ANKRD13A is another one among the top ten down-regulated genes after USP30-AS1 knockdown."
34694569
sparser
"In conclusion, downregulated USP30-AS1 could predict the malignant progression and poor prognosis of colon cancer patients."
35260141
sparser
"According to the expression levels of these 10 prognostic genes (CXCL9, ETV7, GBP4, TRBC2, GBP1P1, CD3E, USP30-AS1, TRBV28, TAP1, and PSMB9), we divided the samples into two groups according to the median expression levels."
34054929
sparser
"This study knocked down and overexpressed USP30-AS1 to determine the roles in AML cell lines."
34694569
sparser
"In our analysis, a total of 6 of the 9 lncRNAs (USP30-AS1, ST7-AS1, VPS9D1-AS1, OTUB6DB-AS1, LINC01087, and MAPT-AS1) were identified."
33614483
sparser
"In our study, USP30-AS1 was considered a tumour suppressor, which may require future in vivo and in vitro experiments to reveal the relationship between USP30-AS1 and cancer."
35031063
sparser
"However, forced expression of USP30-AS1 can enhance the viability of HL-60 cells and reduce apoptosis."
34694569
sparser
"Among these lncRNAs, 5 lncRNAs were unfavorable factors (AC105942.1, AL049840.3, SNHG26, C5orf56, AL139385.1) and 9 lncRNAs were confirmed to be favorable prognostic factors for BLCA (TTC28-AS1, LINC02446, AL662844.4, USP30-AS1, PSMB8-AS1, AL031775.1, AC073534.1, U62317.2, and AJ271736.1) ( xref )."
33289830
sparser
"LncRNA USP30−AS1 had co-expression relationship with 9 ferroptosis-related genes (CYBB, SOCS1, IFNG, TNFAIP3, PML, GCH1, NCF2, SLC2A6, and CAPG), including 4 ferroptosis driver, 2 ferroptosis suppressor and 3 ferroptosis marker, and upregulation of USP30−AS1 in breast cancer tissue was associated with longer overall survival time in this study."
34164433
sparser
"This outcome is in line with survival analysis results, in which high expression of USP30-AS1 was associated with poor prognosis of AML."
34694569
sparser
"Depletion of USP30-AS1 restrains tumour growth in vivo."
33986866
sparser
"For example, USP30-AS1 could regulate the miR-299-3p/PTP4A1 axis resulting in enhancement of cervical cancer progression [ xref ]."
35260141
sparser
"This combination of in silico functional association studies and experimental evidence allowed us to identify a lncRNA signature composed of six elements -NRIR, BISPR, MIR155HG, FMR1-IT1, USP30-AS1, and U62317.2 -associated with the regulation of SARS-CoV-2 infection."
| DOI
sparser
"USP30-AS1 predicts the malignancy and prognosis of colon cancer patients."
35260141
sparser
"Bioinformatics analysis showed that the methylation level at Cg03124318 was negatively correlated with the expression of USP30-AS1 ( r  = − 0.452, *** p  < 0.001, Fig.  xref D)."
34694569
sparser
"There are other genes which are in the vicinity of the USP30-AS1 locus, such as the ACACB , TMEM119, and SSH1 genes (Fig.  xref E)."
34694569
sparser
"The risk score of each specimen of ovarian cancer was calculated as follows: AC084018.1 expression * (−0.008315282) + AC092171.2 expression * 2.84E-05 + AP000695.1 * 0.395692194 + GAS6-AS1 expression * 0.028077942 + LINC00174 expression * 0.010226196 + LINC00893 expression * (−0.013179597) + LINC00996 expression * (−0.214479586) + MEIS1-AS3 expression * (−0.093561265) + MIR22HG expression * (−0.007661823) + NEAT1 expression * (0.000320475) + TEX26-AS1 expression * (−0.061717576) + U73169.1 expression * (−0.263275905) + UBE2Q1-AS1 expression * (−0.589381398) + USP30-AS1 expression * (−0.114206463)."
34671615
sparser
"The ENCODE database reported that the human genome possesses 33,829 lncRNAs ( xref ); however, the regulatory activities of most lncRNAs remain unknown, including USP30-AS1."
33986866
sparser
"USP30-AS1 functions as a ceRNA and upregulates PTP4A1 by decoying miR-299-3p, thereby aggravating the oncogenic potential of cervical cancer cells both in vitro and in vivo ."
33986866
sparser
"In addition, ASH2L was pulled down by the fragment of USP30-AS1 from 250 to 500 bp, and by other fragments containing the region (250–500 bp)."
34694569
sparser
"Results showed that knocking down USP30-AS1 in KG-1 cells resulted in a significant up-regulation of 324 genes (* p  < 0.05 and log 2 FC > 0.585) and a significant down-regulation of 821 genes (* p  < 0.05 and log 2 FC < -0.585, Fig.  xref A and B)."
34694569
sparser
"Furthermore, the expressions of AC105942.1 and MAFG-DT increased notably with the increment of risk scores, while the expressions of AL031775.1, USP30-AS1, AC024060.1, AL162586.1, AP003352.1, PSMB8-AS1, AC016957.2, and STAG3L5P-PVRIG2P-PILRB decreased distinctly, which were in accordance with hazard ratio of these predictors ( xref )."
34281486
sparser
"Taken together, these results suggest that USP30-AS1 functions as a carcinogenic lncRNA in cervical cancer."
33986866
sparser
"The downregulation of USP30-AS1 was associated with the larger tumor size, positive lymph node metastasis, and advanced TNM stage of colon cancer patients, which indicates the severe and malignant progression of patients [ xref ]."
35260141
sparser
"The results showed that in USP30-AS1 (KD) cells, the enrichment of USP30 and ANKRD13A promoters in H3K4me3 and H3K27Ac proteins significantly reduced."
34694569
sparser
"This combination of in silico functional association studies and experimental evidence allowed us to identify a lncRNA signature composed of six elements - NRIR, BISPR, MIR155HG, FMR1-IT1, USP30-AS1, and U62317.2 - associated with the regulation of SARS-CoV-2 infection."
35664076
sparser
"High-throughput sequencing was performed to explore the genes regulated by USP30-AS1."
34694569
sparser
"USP30-AS1 regulates mitochondrial homeostasis and mitophagy in glioblastoma cells."
34653676
sparser
"Among the six lncRNAs, the expression levels of AC092580.4, USP30‐AS1, CTA‐384D8.35, and AC002331.1 were the highest in the high‐infiltrating group, followed by the median‐infiltrating group, and lowest in the low‐infiltrating group."
32096345
sparser
"In the present study, increased USP30-AS1 expression was observed in both cervical cancer tissues and cell lines."
33986866
sparser
"Therefore, the present study aimed to determine the potential functions of USP30-AS1 in cervical cancer and uncover its underlying molecular events."
33986866
sparser
"In the present study, we found that USP30-AS1 mainly distributed in the nucleus of AML cells, implying that the mechanism of USP30-AS1 in AML was mainly related to chromatin and transcriptional regulation."
34694569
sparser
"Among the 9 autophagy-related lncRNAs, USP30-AS1, ST7-AS1, VPS9D1-AS1, TFAP2A-AS1, HOXB-AS1, LINC01087, and MAPT-AS1 are protective factors, while KMT2E-AS1 and OTUB6DB-AS1 are risk-related factors."
33614483
sparser
"In summary, an immune-related lncRNA prognostic evaluation model for EOC was established, which consisted of six lncRNAs (KRT7-AS, USP30-AS1, AC011445.1, AP005205.2, DNM3OS and AC027348.1)."
35031063
sparser
"An oxidative stress-related lncRNA risk model was constructed based on 9 lncRNAs (AC034213.1, AC008124.1, LINC01836, USP30-AS1, AP003555.1, AC083906.3, AC008494.3, AC009549.1, and AP006621.3) by least absolute shrinkage and selection operator (LASSO) analysis."
36869292
sparser
"Additionally, USP30-AS1 (95% CI = 1.089–5.182, P = 0.030) and TNM stage (95% CI = 1.012–3.973, P = 0.046) were identified as independent prognostic factors of colon cancer according to Cox regression analysis (Table xref )."
35260141
sparser
"As indicated by PCR, the knockdown of ASH2L decreased USP30 and ANKRD13A expression (*** p  < 0.001) and impaired the promoting effect of overexpressed USP30-AS1 on USP30 and ANKRD13A expression (Fig.  xref E)."
34694569
sparser
"USP30 antisense RNA 1 (USP30-AS1) has been studied in bladder urothelial carcinoma."
33986866
sparser
"ASH2L knockdown dramatically decreased the enrichment of USP30 and ANKRD13A promoters in despite USP30-AS1 overexpression."
34694569
sparser
"Meanwhile, in a previous ceRNA network of colon adenocarcinoma, USP30-AS1 was identified as a signature lncRNA and was speculated to possess a great potential of predicting patients’ recurrence and prognosis [ xref ]."
35260141
sparser
"Overexpression of ANKRD13A and USP30-AS1 decreased the HLA-1 protein in HL-60 cells (* p  < 0.05)."
34694569
sparser
"Overexpression of ASH2L partially restored the enrichment of USP30 and ANKRD13A promoters in H3K4me3 protein after USP30-AS1 knockdown (Fig.  xref F)."
34694569
sparser
"Among the four cervical cancer cell lines, HeLa and CaSki cells exhibited the highest USP30-AS1 expression levels, as evidenced by RT-qPCR."
33986866
sparser
"Taken together, the miR-299-3p/PTP4A1 axis mediates the central roles of USP30-AS1 in cervical cancer and forms the USP30-AS1/miR-299-3p/PTP4A1 pathway."
33986866
sparser
"Up-regulation of USP30-AS1 in AML associated with poor prognosis."
34694569
sparser
"The association of USP30-AS1 with the clinical features of patients."
35260141
sparser
"Functional experiments, including the Cell Counting Kit-8 assay, flow cytometry, Transwell migration and invasion assays, and mouse tumour model, were performed in order to elucidate the roles of USP30-AS1."
33986866
sparser
"Notably, further investigation determined the effects of USP30-AS1 on PTP4A1 expression in cervical cancer cells."
33986866
sparser
"Therefore, the present study determined whether USP30-AS1 is implicated in cervical cancer malignancy and investigated its relevant underlying molecular mechanisms."
33986866
sparser
"To date, neither the expression status nor the detailed role of USP30-AS1 have been elucidated in cervical cancer."
33986866
sparser
"First, USP30-AS1 expression in CESC was determined using TCGA database."
33986866
sparser
"In summary, USP30-AS1 probably promotes AML cell survival by cis -regulating USP30 and ANKRD13A."
34694569
sparser
"lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer."
35260141
sparser
"The interaction between USP30-AS1 and ASH2L might lead a worse outcome in AML patients."
34694569
sparser
"Here, using the publicly available glioma database, we identified that USP30-AS1, an antisense lncRNA locating on the opposite strand of USP30 locus, is upregulated in human gliomas, particularly in high grade glioma."
34653676
sparser
"Based on these information, we synthesized the full length of USP30-AS1, the antisense of USP30-AS1, as well as the specific regions of the lncRNA to pull down ASH2L."
34694569
sparser
"In xref , we can see 7 lncRNAs are favorable prognostic factors (USP30-AS1, ST7-AS1, VPS9D1-AS1, TFAP2A-AS1, HOXB-AS1, LINC01087, and MAPT-AS1) and 2 lncRNAs are unfavorable prognostic factors (KMT2E-AS1, OTUB6DB-AS1) for breast cancer patients."
33614483
sparser
"The lncRNA signature includes NRIR, BISPR, MIR155HG, USP30-AS1, FMR1-IT1 and U62317.2 noncoding transcripts (Fig. 6a) ."
| DOI
sparser
"LncRNA USP30-AS1 promotes the survival of acute myeloid leukemia cells by cis-regulating USP30 and ANKRD13A."
34694569
sparser
"On this basis, we performed ChIP analysis after knockdown or overexpression of USP30-AS1."
34694569
sparser
"Luciferase reporter and RIP assays were performed to verify the functional interaction between USP30-AS1 and miR-299-3p ( xref )."
33986866
sparser
"For this reason, we selected KG-1 and HL-60 cell lines for loss and gain experimental studies to clarify the effect of USP30-AS1 on the characteristics of AML cells."
34694569
sparser
"The overexpression of USP30-AS1 dramatically suppressed colon cancer cellular processes, which was alleviated by miR-765."
35260141
sparser
"Similarly, anti-miR-299-3p markedly rescued the migration ( xref ) and invasion ( xref ) abilities of HeLa and CaSki cells hindered by USP30-AS1 deficiency."
33986866
sparser
"The downregulation of USP30-AS1 related to the malignant progression and served as an adverse prognostic indicator of colon cancer."
35260141
sparser
"In summary, this study proposed that USP30-AS1 may serve as a valuable prognostic marker for gliomas."
34653676
sparser
"Among the nine FRLs, USP30-AS1 was identified as autophagy-related lncRNA in bladder urothelial carcinoma ( xref ), OC ( xref ), and bladder cancer ( xref ), immune-related lncRNA in cervical cancer ( xref ), and epithelial–mesenchymal transition-related lncRNA in bladder cancer via bioinformatic analysis."
35127813
sparser
"USP30-AS1 was upregulated in cervical cancer tissues when compared with matched normal tissues ( xref )."
33986866
sparser
"Furthermore, a positive correlation was observed between USP30-AS1 and PTP4A1 expression in cervical cancer tissues ( xref )."
33986866
sparser
"To explore the potential molecular basis and regulatory network of USP30-AS1 in promoting cancer, we performed high-throughput sequencing on KG-1 cells with USP30-AS1 knockdown or not."
34694569
sparser
"Interestingly, both USP30 and ANKRD13A genes are located nearby USP30-AS1 genes."
34694569
sparser
"LncRNA levels in whole blood in SARS-CoV-2 patients (Covid19) and non-infected controls (None): g, NRIR; h, BISPR; I, USP30-AS1 and j, AL512306.2."
| DOI
sparser
"USP30-AS1 was mostly localized in the cytoplasm of HeLa and CaSki cells ( xref )."
33986866
sparser
"In the literature mentioned, USP30-AS1 has been identified as a protective factor in cancers which is consistent with our study."
35127813
sparser
"An immune-related lncRNA signature was identified in EOC, which was composed of six immune-related lncRNAs (KRT7-AS, USP30-AS1, AC011445.1, AP005205.2, DNM3OS and AC027348.1)."
35031063
sparser
"Interestingly, Sun et al. found that USP30-AS1 was significantly correlated with CFLAR, CXCR4, DRAM1, IFNG, IKBKE, and TNFSF10 in bladder cancer."
34250091
sparser
"The final risk score calculation formula was as follows: Risk score = expression value of KRT7-AS * 0.2079 + expression value of USP30-AS1 * (− 0.3862) + expression value of AC011445.1 * 0.4593 + expression value of AP005205.2 * (− 0.4020) + expression value of DNM3OS * (0.3120) + expression value of AC027348.1 * (− 0.8224)."
35031063
sparser
"Loss and gain experiments showed that the mRNA and protein expression levels of USP30 and ANKRD13A were positively correlated with the expression of USP30-AS1 in AML."
34694569
sparser
"The regulatory effect of USP30-AS1 on immune escape of AML cells by ANKRD13A/HLA-I is still needed to identify in animal study in the near future."
34694569
sparser
"Meanwhile, the functional roles of USP30-AS1 in tumor cellular processes have also been revealed."
35260141
sparser
"Next, RT-qPCR was performed to measure the expression levels of these candidate miRNAs in cervical cancer cells following USP30-AS1 silencing."
33986866
sparser
"We used AC105942.1, AL031775.1, USP30-AS1, AC024060.1, AL162586.1, AP003352.1, PSMB8-AS1, AC016957.2, STAG3L5P-PVRIG2P-PILRB, and MAFG-DT to construct a hypoxia signature with a robust performance."
34281486
sparser
"USP30-AS1 knockdown significantly inhibited HeLa and CaSki cell proliferation ( xref )."
33986866
sparser
"To assess the potential of lncRNA USP30-AS1 (USP30-AS1) in serving as the biomarker of colon cancer and unearth the underlying mechanism."
35260141
sparser
"Risk score = (0.231 × Expression WNT5A-AS1 ) + (0.420 × Expression AL136084.3 ) + (-0.564 × Expression MIF-AS1 ) + (-0.388 × Expression AC008735.2 ) + (-0.456 × Expression AL357033.4 ) + (-0.661 × Expression LINC00649 ) + (-0.633 × Expression AC099343.2 ) + (-0.263 × Expression USP30-AS1 )."
32579540
sparser
"Here, the downregulation of USP30-AS1 in colon cancer was unearthed."
35260141
sparser
"The overall survival of patients with low USP30-AS1 expression was found to be dramatically lower than that of patients with high USP30-AS1 expression (log rank P = 0.037, Fig. xref )."
35260141
sparser
"Among the eight immune-related lncRNAs selected to construct the prognostic model, MIR205HG, AC004847.1 and AL590764.1 were poor outcome risk genes, while U62317.1, USP30-AS1, AL133371.2, AL365361.1 and HLA-DQB1-AS1 were protective genes."
33675585
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"The results showed that FRL CTC-246B18.8 was significantly upregulated in OC cells and samples ( xref ), while FRLs SNHG10 and USP30-AS1 were downregulated compared with normal control ( xref ."
35127813
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"The inhibitory effect of USP30-AS1 on cell growth and metastasis of colon cancer was observed in the present study, suggesting its tumor inhibitor role in colon cancer development."
35260141
sparser
"USP30-AS1 was identified as a cytoplasmic lncRNA in cervical cancer cells."
33986866
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"In order to investigate the carcinogenic mechanism of USP30-AS1 in cervical cancer, the subcellular distribution of USP30-AS1 was examined via a subcellular fractionation assay."
33986866
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"In conclusion, to the best of our knowledge, the present study demonstrated for the first time that high USP30-AS1 expression is closely associated with worse overall survival outcomes in patients with cervical cancer."
33986866
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"Among the six lncRNAs, the expression levels of AC092580.4, USP30‐AS1, CTA‐384D8.35, and AC002331.1 were positively correlated with the abundance of most immune cells and the remaining lncRNAs were negatively correlated with the abundance of most immune cells (Figure xref A)."
32096345
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"As shown in Fig.  xref A, among these cell lines, USP30-AS1 has the highest expression in KG-1 cells and the lowest expression in HL-60 cells."
34694569
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"The USP30-AS1 lncRNA is an antisense transcript to the USP30 gene that has been implicated in mitochondrial quality control in some cancers and also in the progression of virus-induced cancers such as malignant cervical tumors [55, 56] , and is mainly upregulated in those SARS-CoV-2 patients with higher viral loads."
| DOI
sparser
"USP30-AS1 targets USP30 to promote cancer in AML cells."
34694569
sparser
"Results showed that USP30-AS1 promoted AML cell viability and inhibited apoptosis."
34694569
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"To explore the clinical significance of the epigenetic changes of USP30-AS1 gene in AML, we detected multiple methylation sites on the USP30-AS1 gene promoter from the TCGA database (illuminaMethyl450_hg38_GDC)."
34694569
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"Furthermore, patients with cervical cancer that exhibit high USP30-AS1 expression levels had shorter overall survival than those with low USP30-AS1 expression levels."
33986866
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"Furthermore, the RIP assay revealed that both USP30-AS1 and miR-299-3p had a tendency to be present in Ago2-rich magnetic beads ( xref )."
33986866
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"The nine lncRNAs we screened were AC084018.1, AP001007.1, DICER1-AS1, HCP5, LBX2-AS1, LINC00515, MAPT-AS1, USP30-AS1, and MIR155HG."
33391355
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"Furthermore, compared with Ect1/E6E7 cells, USP30-AS1 levels were increased in the tested cervical cancer cell lines ( xref )."
33986866
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"The target of USP30-AS1 was predicted using bioinformatics analysis, which was further verified via RNA immunoprecipitation and luciferase reporter assays."
33986866
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"A total of 14 lncRNAs were identified as protective genes (AC022150.4, AC061992.1, AC090948.3, AC092794.1, AC107464.3, AL021707.8, AL451085.2, AL606834.2, FLJ42351, LINC00926, LINC01871, TNFRSF14-AS1, U73166.1 and USP30-AS1) with HRs < 1 while 10 lncRNAs (AC022150.2, AC090948.1, AC243960.1, AL021707.6, ITGB2-AS1, OTUD6B-AS1, SP2-AS1, TOLLIP-AS1, Z68871.1 and ZNF337-AS1) were associated with increased risk with HRs >1."
35441740
sparser
"Therefore, the cis -regulation of USP30-AS1 is partially associated with ASH2L. It has been reported that some proteins in TrxG complex are recruited by lncRNA to gene promoter to stimulate gene expression."
34694569
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"USP30-AS1 is a lncRNA, which can regulate the expression of its neighboring genes (usually within 1 million bps from the lncRNA gene), that is, cis-acting."
34694569
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"Among them, USP30 and ANKRD13A are genes close to USP30-AS1 gene in chromosome."
34694569
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"Patients with high USP30-AS1 expression levels had shorter overall survival times than those with low USP30-AS1 expression ( xref )."
33986866
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"The methylation level at Cg03124318 was negatively correlated with the expression of USP30-AS1."
34694569
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"USP30-AS1 is upregulated in bladder urothelial carcinoma and is associated with cell autophagy ( xref )."
33986866
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"USP30-AS1 induced HLA-I internalization from the cell membrane by up-regulating ANKRD13A, which might induce the immune escape of AML cells."
34694569
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"USP30-AS1 is mainly involved in the biological process of AML cells through chromatin regulation, and plays a role in promoting cancer."
34694569
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"The dysregulation of USP30-AS1 was observed in a lncRNA expression profile of colorectal cancer [ xref ]."
35260141
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"Further, the 52 autophagy-related lncRNAs mentioned above were analyzed by multivariate Cox analysis with R. Fifteen lncRNAs were selected: LINC01943, AC090948.3, USP30-AS1, AC068282.1, AC004687.1, AL133371.2, AC242842.1, PCED1B-AS1, HLA-DQB1-AS1, AC011374.2, LINC00324, AC018553.1, LINC00520, DBH-AS1, and ITGB2-AS1."
35611195
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"USP30-AS1 promotes ANKRD13A to induce the translocation of HLA-1 from the cell membrane to the cytoplasm."
34694569
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"Results: The prognostic model was developed based on the lncRNA group including LIPE-AS1, AC124067.4, LINC01655, AP005131.3, AC015802.3, USP30-AS1, SNHG26, and AL589765.4."
35646057
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"LncRNA expression in nasal swabs distributed by groups of patients: a, NRIR; b, LINC02068; c, BISPR; d, USP30-AS1; e, MIR155HG and f, AL512306.2."
| DOI
sparser
"USP30-AS1 expression is related to autophagy in the bladder urothelial carcinoma ( xref )."
34671615
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"After USP30-AS1 knockdown in AML cells, forced USP30 gene can rescue the decreased cell viability and increased cell apoptosis."
34694569
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"Meanwhile, the downstream mechanism of USP30-AS1 was also disclosed."
35260141
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"To analyse the effects of USP30-AS1 on tumour growth in vivo , HeLa cells stably expressing sh-USP30-AS1 were constructed and subcutaneously injected into nude mice to construct a mouse tumour model."
33986866
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"Our results showed that when the expression of USP30-AS1 was down-regulated, the viability of KG-1 cells decreased significantly and the apoptotic rate increased."
34694569
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"These six IR-lncRs were used in the prognostic model construction, and they were KRT7-AS, USP30-AS1, AC011445.1, AP005205.2, DNM3OS and AC027348.1, and the corresponding coefficients were also given (Table  xref )."
35031063
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"To investigate whether USP30-AS1 acts as a miRNA sponge, the exact distribution of USP30-AS1 in cervical cancer cells was determined via a subcellular fractionation assay."
33986866
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"High-throughput sequencing reveals genes regulated by USP30-AS1."
34694569
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"USP30-AS1 promotes the viability of AML cells and suppresses apoptosis."
34694569
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"ASH2L was pulled down by the full length of USP30-AS1, but hardly by the antisense of USP30-AS1 (Fig.  xref C)."
34694569
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"USP30-AS1 depletion inhibits cervical cancer cell proliferation, migration and invasion, and promotes cell apoptosis in vitro."
33986866
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"Therefore, the present study determined whether USP30-AS1 is implicated in cervical cancer malignancy, and investigated relevant molecular mechanisms."
33986866
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"As indicated by catRAPID (), ASH2L is predicted to bind to many regions of USP30-AS1, but there regions are mostly located within the 250–550 bp of USP30-AS1 (Table xref and Fig.  xref A and B)."
34694569
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"It was found that a big proportion of patients with a relatively larger tumor size showed a low expression of USP30-AS1 (62.5 vs. 37.5%, Fig. xref A)."
35260141
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"Therefore, USP30-AS1 can be an important lncRNA target for AML treatment."
34694569
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"As indicated by western blot, knockdown of ANKRD13A and USP30-AS1 increased the HLA-1 protein in KG-1 cells (* p  < 0.05, Fig.  xref D)."
34694569
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"Similar to USP30-AS1, overexpression of a fragment of USP30-AS1 (250–550 bp) also increased the enrichment of USP30 and ANKRD13A promoters in H3K4me3 protein (* p  < 0.05)."
34694569
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"At the same time, USP30-AS1 can also target ANKRD13A to affect the translocation of HLA-Iprotein from cell membrane to cytoplasm, probably leading to the tumor immune escape."
34694569
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"Therefore, the correlated function miRNAs involved in t USP30-AS1’s function in colon cancer progression are also of great significance to understand the potential of USP30-AS1 in serving biomarkers of colon cancer."
35260141
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"USP30-AS1 competitively sponges miR-299-3p to positively regulate PTP4A1 expression in cervical cancer."
33986866
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"Finally, we found 11 lncRNAs (AC108474.1, AL133467.1, LINC01235, AC072039.2, AL365356.1, AC012213.3, TDRKH-AS1, USP30-AS1, MAPT-AS1, AC092916.1, and LIPE-AS1) that were present in all three sets of differentially expressed lncRNAs, prognostic lncRNAs and ferroptosis-related lncRNAs ( xref )."
34164433
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"The clinical significance of USP30-AS1 was assessed with a series of statistical methods, while the CCK8 and Transwell assay were conducted to estimate its biological effect on the colon cancer cellular processes."
35260141
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"For instance, the HCP5 and PSMB8-AS1 were related to the Antigen Processing and Presentation, Antimicrobials, and Natural Killer Cell Cytotoxicity; AL133264.2 was related to Interleukins; the LINC01684 and USP30-AS1 were related to Antimicrobials."
33584801
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"Long non-coding RNA USP30-AS1 aggravates the malignant progression of cervical cancer by sequestering microRNA-299-3p and thereby overexpressing PTP4A1."
33986866
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"As indicated by immunoprecipitation, knockdown of ANKRD13A or USP30-AS1 decreased the enrichment of ANKRD13A in HLA-1 protein in KG-1 cells; overexpression of ANKRD13A or USP30-AS1 conversely increased the enrichment of ANKRD13A in HLA-1 protein in HL-60 cells (Fig.  xref B)."
34694569
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"It can judge that the hypomethylation of Cg03124318 might lead to the high expression of USP30-AS1 in AML, whereby associating to poor prognosis."
34694569
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"Lasso regression analysis is conducted to construct a prognostic model using eight lncRNAs (USP30-AS1, AC097662.1, AC007686.3, AL133467.1, AP006284.1, NDUFA6-DT, LINC01871, AL135818.1)."
35528236
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"Therefore, the up-regulation of ANKRD13A by USP30-AS1 contributed to HLA-I internalization."
34694569
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"Furthermore, we knocked down and overexpressed USP30-AS1 to determine the functions in AML cell lines."
34694569
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"Among these genes, the expressions of USP30 and ANKRD13A were positively regulated by USP30-AS1, while the expression of ACACB was negatively regulated by USP30-AS1 (* p  < 0.05, ** p  < 0.01, or *** p  < 0.001, Fig.  xref A and B)."
34694569
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"By searching the miRDB, a total of 41 miRNAs ( xref ) were predicted as potential downstream targets of USP30-AS1."
33986866
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"Seventeen differentially expressed lncRNAs (AC010186.3, AC006001.2, LBX2-AS1, SNHG17, AC011445.1, AC083880.1, MIR193BHG, AC025259.3, HCG14, AC007114.1, AC108673.2, USP30-AS1, AC010336.5, LINC01132, AC006333.2, LINC00665 and AC027348.1) were selected as independent prognostic factors for OC patients."
33179541
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"These results indicated that USP30-AS1 promotes the survival of AML cells."
34694569
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"The expression levels of only three genes ( USP30 , ACACB, and ANKRD13A ) were significantly impacted with the knockdown and overexpression of USP30-AS1 in both KG-1 and HL-60 cells."
34694569
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"We also explored the interaction of USP30-AS1 with the adjacent genes USP30 and ANKRD13A to reveal the mechanism underlying the carcinogenic role of USP30-AS1 in AML."
34694569
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"The cell viability test and apoptosis results showed that the reduction of USP30-AS1 expression inhibited the proliferation of KG-1 cells and increased the rate of apoptosis."
34694569
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"With the aim of further exploring the regulatory mechanism of USP30-AS1 on USP30 and ANKRD13A, we designed a FISH test which found that USP30-AS1 was mainly present in the nucleus (Fig.  xref B)."
34694569
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"Since immune cells play important roles in T2D, we conducted loss-of-function experiments to provide functional data on the T2D-related lncRNA USP30-AS1 , using an in vitro model of pro-inflammatory macrophage activation."
37218990
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"Based on the median value of USP30-AS1, all patients with cervical cancer were classified into two groups: USP30-AS1-high (n=28) and USP30-AS1-low (n=28) expression groups."
33986866
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"Overexpression of ANKRD13A and USP30-AS1 individually decreased HLA-1 protein in the cell membrane in HL-60 cells, compared to control cells; the effect exerted by USP30-AS1 overexpression was abolished by ANKRD13A knockdown; Knockdown of ANKRD13A and USP30-AS1 individually was associated with high level of HLA-1 protein in the cell membrane of HL-60 cells."
34694569
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"Herein, USP30-AS1 overexpression was detected in cervical cancer sample data from The Cancer Genome Atlas and our cohort."
33986866
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"Therefore, USP30-AS1 may participate in the process of autophagy by affecting ARGs."
34250091
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"USP30 is the gene that had the most significant down-regulation after USP30-AS1 knockdown."
34694569
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"USP30-AS1 transcript is related to autophagy and mitochondrial quality control in the context of tumor progression [56, 70] , whereas FMR1-IT1 and U62317.2 have no characterized functions."
| DOI
sparser
"The regulatory role of USP30-AS1 in cancer has not been thoroughly studied."
35031063
sparser
"The present study attempted to further understand the relationship among USP30-AS1, miR-299-3p and PTP4A1 in cervical cancer."
33986866