IndraLab

Statements

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"Conversely, overexpression of USP11 specifically inhibited viral genomic RNA replication, and this inhibition required the deubiquitinase activity."
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"When cellular USP11 was knocked down, the amounts of vRNA and cRNA of the wild-type virus increased by about 2.5-fold ( Figure 7A, lane 1 versus lanes 2 and 3) , indicating that USP11 inhibits viral RNA replication as previously mentioned; with the mutant virus defective in NP ubiquitination (K184R), however, there was no significant difference in viral RNA synthesis between USP11 knockdown and control cells ( Figure 7A, lanes 4-6) ."
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"This result further supports the conclusion that USP11 inhibits viral RNA replication through deubiquitinating NP.Ubiquitination is a posttranslational modification, in which ubiquitin chains or single ubiquitin molecules are linked to target proteins, giving rise to poly-or monoubiquitination (Weissman, 2001) ."
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"Significantly, USP11 can cleave single ubiquitin from NP, and thereby inhibit the RNA replication efficiency ( Figure 8B )."
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"USP11 inhibits viral RNA replication through deubiquitinating on K184 of NP."
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