IndraLab

Statements

USP10 binds CKO. 13 / 13
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"USP10-CKO mice were constructed by Shanghai Model Organisms through CRISPR-Cas9 technology."
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"Western blot analysis demonstrated a specific Sirt6 decrease in the hearts of USP10‐CKO mice 4 weeks after AB surgery, whereas Sirt6 was upregulated when USP10 was overexpressed (Figure  xref and xref )."
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"Cardiac‐specific USP10 knockout (USP10‐CKO) mice and USP10‐transgenic (USP10‐TG) mice were used to examined the role of USP10 in CH following aortic banding."
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"Moreover, hematoxylin and eosin and Picrosirius red staining of heart sections indicated that compared with those of their USP10‐Flox littermates, hearts from USP10‐CKO mice developed significant cardiomyocyte hypertrophy and fibrosis in the interstitial and perivascular spaces (Figure  xref through xref )."
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"Furthermore, the USP10‐CKO mice exhibited significantly increased LVEDd and LVESd values and decreased fractional shortening% and ejection fraction values compared with USP10‐Flox controls (Figure  xref through xref )."
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"Subsequently, USP10‐Flox and USP10‐CKO mice were subjected to AB surgery, and cardiac parameters were evaluated."
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"In addition, myocardial fibrosis in perivascular and interstitial spaces were significantly increased in USP10-CKO mice ( xref )."
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"Western blotting results demonstrated that phosphorylation levels of Akt, GSK3β, mTOR, and p70‐S6K were significantly increased in hypertrophic hearts in USP10‐CKO mice compared with those in USP10‐Flox controls in response to AB surgery (Figure  xref )."
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"As shown in Figure  xref , the ratios of heart weight (HW) to body weight (BW) increased in USP10‐CKO mice compared with USP10‐Flox group at 4 weeks after AB surgery (Figure  xref )."
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"First, we tested Sirt6 level in USP10‐CKO and USP10‐TG mice."
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"Additionally, to clarify the effect of USP10 on CH in vivo, we established cardiac‐specific USP10 knockout mice (USP10‐CKO)."
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"Methods and Results Cardiac-specific USP10 knockout (USP10-CKO) mice and USP10-transgenic (USP10-TG) mice were used to examined the role of USP10 in CH following aortic banding."
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"In parallel, the mRNA levels of CH‐ and fibrosis‐related genes, including Anp, Bnp, Myh7, collagen Iα, collagen III, and Ctgf, were markedly increased in the hearts of USP10‐CKO mice compared with those in the hearts of USP10‐Flox controls (Figure  xref )."
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