IndraLab

Statements

DCC is methylated. 104 / 104
| 104
sparser
"Recent data suggests that detection of promoter methylation of EDNRB and DCC in patients with high-risk oral lesions has a similar performance in diagnosis as expert clinical evaluation [ xref ]."
26544568
sparser
"ESR1 and DCC methylation were significantly higher in HPV16-positive women."
22664184
sparser
"When we categorized a cell line showing aΔC T of more than 15.0 as DCC expression-negative and a cell line with aΔC T of 15.0 or less as DCC expression-positive, only the GCIY cell line could be categorized as DCC expression-positive among the seven gastric cancer cell lines with DCC methylation; this finding was statistically non-significant."
26207151
sparser
"Seven of 8 cases with complete loss, 33 of 38 cases with focal loss, and 34 of 40 cases with positive DCC expression were informative for both DCC promoter methylation and 18q LOH."
26207151
sparser
"The CGI spanning promoter/exon 1 of DCC was methylated at Taq I site in 8 out of 10 tumors as demonstrated by almost complete digestion with Taq I whereas it was methylated in matching normal colon tissues only in sample #8 and #10 ( xref ."
20454457
sparser
"While TBX5 and DCC are methylated in all cell lines, other genes ( TBX18, DGKI, PGRMC1, LIN7C and HOXB13 ) are differentially methylated ( xref )."
20454457
sparser
"There was very little evidence of any significant associations of clinical characteristics with methylation of p16 , DCC , CCNA1 , CD1A , GADD45α , NDN or LINE-1 genes."
30862188
sparser
"The methylation of Kif1a, DCC, RarB, and NISCH were examined in these groups ( xref )."
20592015
sparser
"In terms of RPFT, there were significant associations with CCNA1 , DCC and NDN methylation in all HNSCC patients."
26518708
sparser
"Recently, two reports indicated that UNC5C and DCC methylation was closely associated with loss of gene expression in colorectal cancer."
19242752
sparser
"DCC, DLC-1 and LRP1B were identified as tumor suppressor genes and were aberrantly methylated in cancer cells ( xref – xref )."
21239472
sparser
"For patients without methylated DCC, addition of a logistic regression score that was based on the five remaining genes improved sensitivity from 35.5% to 75% but decreased the specificity from 100% to 73%."
21610147
sparser
"In tumors of never smokers, CCNA1 methylation was higher (p<0.04) while DCC methylation tended to be lower (p=0.18)."
30862188
sparser
"Tobacco use was significantly associated with methylation of NDN , CD1A , and DCC ( p-value  = 0.02; p-value  < 0.001; p-value  = 0.03, respectively)."
26518708
sparser
"Similar to the observations for DCC methylation, higher ESR1 methylation at specific sites was associated both with abnormal cytology and HPV 16 positivity."
22664184
sparser
"Similarly, the plasma methylation of SEPT9 , DCC , BOLL , and SRFP2 showed stronger correlation with tumor burden than CEA and CA-19-9 [ xref ]."
34208598
sparser
"Finally, methylation of NDN and DCC was significantly higher in males compared to females ( p-value =0.003; p-value =0.002, respectively)."
26518708
sparser
"Recently, we examined the methylation status of the DCC gene in colorectal carcinomas and found that aberrant methylation of the DCC gene was detected in 28 out of the 50 (56%) primary colon carcinomas."
20150623
sparser
"However, experiments in the future need to confirm if methylated DCC can serve as a marker during the screening of cervical cancer ( xref )."
32328088
sparser
"Primary oral cancer patients with methylated DCC had a significantly reduced survival than those who did not have DCC methylation. xref The presence ofp16 INK4a or DCC methylation in the primary tumor has been shown to be associated with poor outcome. xref , xref , xref Our study supports these findings."
20473870
sparser
"Interestingly, DCC methylation has been associated with head and neck squamous cell carcinoma xref and in one study, was found to be differentially methylated in head and neck cancer cell lines by HPV status xref ."
28500655
sparser
"However, DCC methylation was not associated with any clinical or pathologic parameters measured."
18302152
sparser
"CCNA1, DAPK, DCC and TIMP3 methylation were highly specific for being found in the OSCC samples."
25686481
sparser
"In our previous study ( xref ), DCC methylation was detected in 54% of cases with 100% specificity in evaluation set and fell to 26% in the independent set."
21610147
sparser
"Methylation of CCNA1 , NDN , CD1A , and DCC was higher, while methylation of p16 was lower, in HPV (+) tumors compared to HPV (-) tumors."
26518708
sparser
"In this study we examined methylation of DCC, KIF1A, EDNRB, and p16(INK4a) in a large cohort of HNC patients from Eastern Cooperative Group (ECOG) 4393/Radiation Therapy Oncology Group (RTOG) 9614 to identify clinical correlates of methylation of these genes."
23444219
sparser
"DCC methylation was detected in 16/47 (34%) HIV-NHL, and was more frequent in HIV-DLBCL (12/28; 43%) compared to HIV-BL (3/11; 27%), and HIV-PEL (1/8; 13%)."
19832807
sparser
"Nonetheless, tests utilizing DCC and EDNRB promoter methylation is limited to 46% sensitivity, and 72% specificity for cancer detection in salivary rinses [ xref ]."
26544568
sparser
"Further studies are needed to determine whether methylation of DCC can serve as a triage marker for cervical cancer screening."
28500655
sparser
"In CRCs, UNC5C and DCC methylation is associated with loss of gene expression [ xref , xref ]."
22112479
sparser
"DCC methylation status was significantly associated with MSI status."
26207151
sparser
"Shin et al. reported the promoter methylation of MOS, DCC, CRK , and PTPN6 in gastric cancer [ xref ]."
28418867
sparser
"Therefore, we defined a DCC methylation of 5 % or more as a continuous variable (i.e., >5.0 % methylation was defined as methylation-positive (methylated) and <5.0 % methylation as methylation-negative (unmethylated))."
26207151
sparser
"In addition, methylation of DCC was correlated with bone invasion by gingival tumors (P = 0.036), with aggressive invasiveness of tumors of the tongue (P = 0.046), and with reduced survival (P = 0.050)."
12374684
sparser
"DCC methylation in Q2 was associated with the highest probability of OST ( p -value = 0.005)."
26518708
sparser
"SSTR1 methylation was significantly correlated with tumor size ( P = 0.037), stage ( P = 0.037), galanin ( P = 0.030), GALR2 methylation ( P = 0.014), TAC1 methylation ( P = 0.023), TAC1R methylation ( P = 0.003), H-cadherin methylation ( P = 0.007), MGMT methylation ( P = 0.001), DAPK methylation ( P = 0.001) and DCC methylation ( P = 0.045) ( xref and xref )."
25734919
sparser
"Of note, methylation of p16, DCC, EDNRB and KIF1A varies across the amount of tobacco used ( xref )."
23444219
sparser
"We have demonstrated that DCC methylation is a frequent and cancer-specific event in primary ESCCs, suggesting that DCC and associated pathways may represent a new diagnostical therapeutic target."
18302152
sparser
"Sato et al. [33] reported previously that DCC gene methylation occurred in 75% of primary gastric tumors and that the methylation status was significantly correlated with loss of DCC expression."
16122883
sparser
"For patients without methylated DCC, addition of a logistic regression score that was based on the five remaining genes improved sensitivity from 35.5% to 75% (95% CI: 64%, 84%) but decreased the specificity from 100% to 73% (95% CI:54%, 88%)."
21610147
sparser
"DCC gene methylation was significantly different between plasma from controls, GGO, and lung cancer patients 4/80 (5%), 0/23 (0%) and 19/70 (26%), respectively (p=0.0002)."
20592015
sparser
"Interestingly, only subjects with an abnormal cytology had high (>16%) mean levels of DCC methylation across the five sites analyzed ( xref ), although this was true only for six of those with abnormal cytology (range: 17.0–70.2%), all HPV 16 positive."
22664184
sparser
"Further, when different combinations of genes were evaluated, OSCC harboring both p16 and DCC methylation emerged to be more significantly associated with nodal involvement than p16 alone ( p = 0.005, OR = 19.2, 95% C.I. =2.4–152.8; xref )."
20473870
sparser
"There were no significant differences in overall methylation of CCNA1, DCC, CD1A or NDN within paired specimens ( p -values= 0.56, 0.17, 0.66 and 0.58, respectively)."
26566081
sparser
"For patients without methylated DCC, addition of a logistic regression score that was based on the five remaining genes improved sensitivity from 35.5% to 75% (95% CI: 64%, 84%) but decreased the specificity from 100% to 73% (54%, 88%) ( xref )."
21610147
sparser
"Next, we investigated the association between DCC promoter methylation and various clinicopathological and genetic features."
26207151
sparser
"On multivariable modeling, histological diagnosis was only independently associated with EDNRB (p=0.0003) or DCC (p=0.004) methylation."
23637120
sparser
"A significant reduction in the DCC methylation pattern was observed in the valproic group (p = 0.023)."
34385507
sparser
"When methylation of at least one of Kif1a , DCC , RARB , or NISCH was considered, methylation frequency was smoking-dependent; while none of the light- or non-smoker controls showed plasma DNA methylation, the cumulative smoking dose (pack-years) correlated well with the methylation frequency in cancer-free heavy smokers ( xref )."
22989067
sparser
"When a qMSP cut-off value for positivity was set to 1.0, DCC methylation was detected in 10 of 12 ESCC cell lines tested, 74% of primary ESCCs (n = 70), 0% of corresponding normal esophageal tissues (n = 20) and 0% of normal esophagus from healthy individuals (n = 19)."
18302152
sparser
"In addition, positive correlation with TIMP3 and a marginal correlation with DCC methylation were observed."
22694820
sparser
"DCC is a tumour suppressor and both deletion and promoter methylation of DCC are involved in its frequent downregulation in colorectal cancer ( xref ; xref )."
26335606
sparser
"Taylor et al. found methylation in DCC in 9 of 10 cases of precursor B ALL and in RUNDC3B , KCNK2 , and DLC1 in 7, 7 and 8 (respectively) of these 10 cases [ xref ]."
20573277
sparser
"In combining the results from both DCC and EDNRB methylation assays, we observed improvement in performance, indicating their potential as biomarkers for HNSCC."
23637120
sparser
"There were no significant associations between DCC methylation status and any other variables."
26207151
sparser
"The impact ofp16 INK4a and DCC methylation in the primary tumor on nodal involvement was not related to histology or the tumor size."
20473870
sparser
"The presence of EDNRB and/or DCC promoter methylation in salivary rinses compares well to examination by an expert clinician in risk classification of oral premalignant and malignant lesions."
23637120
sparser
"We investigated DCC methylation status in 98 gastric cancers and 105 normal gastric mucosa specimens."
26207151
sparser
"In the salivary rinse samples collected without brush, primary tumor site was associated promoter methylation of CCNA1 ; and clinical TNM stage was associated with promoter methylation of CCNA1 and DCC ( xref )."
22438973
sparser
"Of these six subjects with high average DCC methylation, 1 had atypical squamous cells of undetermined significance, 2 had low grade squamous intraepithelial lesions, 2 had high grade squamous intraepithelial lesions and 1 had atypical glandular cells cytology."
22664184
sparser
"A multivariate model analyzed risk classification and EDNRB, HOXA9 and DCC methylation status."
23637120
sparser
"DNA methylation of DCC , EDNRB , DAPK , CCNA1 , p16 , HOXA9 and KIF1A genes have been detected in primary tissues and biological fluids, including saliva and plasma [ xref , xref , xref , xref ], derived from HNSCC patients."
26544568
sparser
"DCC methylation was highly variable, and there were six subjects with notably high mean levels of DCC methylation and corresponding abnormal cytology (of which 1 was atypical squamous cells of undetermined significance, 2 were low grade squamous intraepithelial lesions, 2 were high grade squamous intraepithelial lesions and 1 was atypical glandular cells), indicating that DCC may be another promising marker of abnormal cytology."
22664184
sparser
"Promoter methylation of SFRP and DCC was elevated in T-47D-HNO relative to its parent cell line (p<0.05)."
31882476
sparser
"Among the 58 cancers with DCC alterations, 17 tumors showed alterations in both DCC methylation and 18q LOH, 19 had 18q LOH alone, and 22 cancers exhibited DCC methylation alone."
26207151
sparser
"EDNRB and/or DCC methylation in salivary rinses compares well to examination by an expert clinician in CRC of oral lesions."
23637120
sparser
"DCC methylation was correlated with bone invasion, and deep invasion of the tongue."
20473870
sparser
"A similar pattern was observed for DCC methylation and RPFT, although with DCC , it was Q1 had much poorer probability of recurrence/persistence compared to other quartiles ( p-value  = 0.002). (Additional file xref : Figure S4)"
26518708
sparser
"In OSCC, different levels of methylation have been reported for the p16 , p15 , p14 , DCC , DAPK , MINT1 , MINT2 , MINT27 , and MINT31 gene promoters and DCC methylation was associated with bone invasion of gingival tumors, invasiveness, and reduced survival (Ogi et al. xref )."
22704334
sparser
"When we categorized gastric cancers with LOH ratios higher than 0 as CIN-positive, 12/17 gastric cancers (71 %) with both DCC methylation and 18q LOH, 13/17 gastric cancers (76 %) with 18q LOH alone, 12/22 gastric cancers (55 %) with DCC methylation alone, and 6/25 cancers (24 %) negative for DCC alterations were categorized as CIN-positive ( P  = 0.0025, Pearson’s chi-square test)."
26207151
sparser
"Deleted in colorectal cancer was validated as a tumor marker in head and neck cancer xref as well as esophageal SCC. xref Deleted in colorectal cancer is present on chromosome 18q, suppresses epithelial cell malignant phenotype, xref and is involved in apoptosis. xref It appeared to be a highly specific assay as remarkably aberrant methylation of DCC promoter was not observed in any of the control plasma samples, with AUC ROC curve to be 0.76 (95% CI: 0.641-0.88, P < ."
| PMC
sparser
"Patients with exfoliated tumors cells positive for DCC methylation showed a trend toward a lower local recurrence-free survival."
23595968
sparser
"Overall, 70 % (58 of 83 informative cases) and 51 % (40 of 79 informative cases) of gastric cancers harbored either LOH or aberrant methylation in the DCC and UNC5C genes, respectively."
26207151
sparser
"DNA methylation of PTPN6, MOS, DCC, CRK, and VAV1 was evaluated in non-neoplastic gastric specimens using quantitative methylation-specific PCR in patients with GC (n = 207) and their age- and gender-matched controls (n = 207)."
22252584
sparser
"Aberrant promoter methylation of DCC as determined by quantitative methylation-specific PCR showed significant differences in DCC promoter methylation status of patients with lung cancer and controls ( P = ."
| PMC
sparser
"DCC methylation was lower in the valproic acid group than in the placebo group."
34385507
sparser
"The methylation of DCC in fcDNA was associated with age (r s =0.254; P = ."
| PMC
sparser
"Determination of Aberrant Promoter Methylation of DCC."
| PMC
sparser
"Methylation of DCC was correlated with aggressive phenotype for HNC in a study of 96 tumors with increased rate of bone invasion, invasive growth pattern and reduced survival (p=0.050). ( xref ) Intriguingly, in this study, methylation of p14ARF was associated with a good prognosis (HR =0.30 for methylated tumors p=0.021), perhaps implicating a mechanism similar to the protective effect of p16 methylation in our cohort."
23444219
sparser
"The authors observed the dynamic changes in methylated SEPT9 and DCC genes during neoadjuvant chemotherapy."
32599894
sparser
"The LOH ratio calculated for the other seven loci was significantly highest in gastric cancers with both DCC methylation and 18q LOH (mean LOH ratio, 0.44; SD ±0.39) and in cancers with 18q LOH alone (mean LOH ratio, 0.44; SD ±0.34), intermediate in cancers with DCC methylation alone (mean LOH ratio, 0.16; SD ±0.18), and lowest in cancers negative for DCC alterations (mean LOH ratio, 0.05; SD ±0.10; P  < 0.0001, Wilcoxon/Kruskal–Wallis test)."
26207151
sparser
"MSI-positive gastric cancers were significantly more frequently associated with DCC methylation than with DCC unmethylation (23 vs. 6 %, P  = 0.013; Table  xref )."
26207151
sparser
"In a recent study of women undergoing routine cervical cancer screening in Appalachian Ohio, pyrosequencing of candidate genes revealed an association of DCC methylation with increased odds of abnormal cytology xref ."
28500655
sparser
"HOXA9 , EDNRB and DCC methylation were associated (p= 0.012; p<0.0001; p=0.0005) with premalignant or malignant disease."
23637120
sparser
"However, methylation of the DCC gene has recently been found in noncolorectal tumors."
19394332
sparser
"DCC methylation was observed in the course of gastric carcinogenesis and disappeared in advanced gastric carcinoma."
20150623
sparser
"We report CCNA1 and DCC methylation levels similar to previous studies( xref – xref )."
26566081
sparser
"DCC and EDNRB methylation status had again a significant association with a dysplasia/cancer diagnosis (OR=2.5, 95%CI=1.1-6.0; p=0.037 and OR=2.1, 95%CI=1.0-4.6; p=0.047, respectively) after adjusting for risk classification."
23637120
sparser
"Here, we found up-regulated methylation of NTN3, NTN4, NTN5, and NTNG1 and downstream genes UNC5C, UNC5D, DCC, and TP53 in breast cancer."
32251318
sparser
"Ogi et al . xref showed promoter methylation of DCC gene in 16 of 96 primary OSCC."
20473870
sparser
"The results of this study indicate that in many hematological cell lines, the DCC, p51, and MGMT genes have been abnormally methylated in the CpG region."
16247226
sparser
"SSTR1 methylation was significantly correlated with tumor size, stage, and methylation of galanin , GALR2 , TAC1 , TAC1R , H-cadherin , MGMT , DAPK , and DCC methylation [ xref ]."
26251921
sparser
"Aberrant methylation of the DCC gene was detected in 16 out of the 36 (44%) primary gastric carcinomas."
20150623
sparser
"This analysis showed that TGFβR2, CALCA , HIC1 , SOCS1, RARβ, COX2 , CDH1 , THBS1 , HIN1 , CDKN2B , UCHL1 , CCND2 , MT1G and DCC were frequently methylated in control samples, showing low specificity (Table  xref )."
24359512
sparser
"In this study, we analyzed esophageal squamous cell carcinoma (ESCC) cell lines and primary ESCCs as well as normal esophageal tissues for DCC methylation by bisulfite sequencing, methylation-specific PCR (MSP) and/or quantitative methylation-specific PCR (qMSP)."
18302152
sparser
"Although the presence of DCC methylation alone was not a predictor of nodal involvement, combination withp16 INK4a emerged to be more significantly associated thanp16 INK4a alone."
20473870
sparser
"Similarly to the DCC methylation status, we investigated associations between 18q LOH status and various clinicopathological and genetic features."
26207151
sparser
"In the salivary rinse samples collected with brush, smoking status was associated with promoter methylation of TIMP3 ; HPV status was associated with promoter methylation of CCNA1 , DCC , and MGMT ; primary tumor site (Oral cavity) was associated with promoter methylation of CCNA1 , DCC and DAPK ; clinical TNM stage were associated with promoter methylation of P16 , DCC , and MINT31 ; and pathological nodal stage was associated with promoter methylation of P16 and MINT31 ( xref )."
22438973
sparser
"DCC methylation has been identified as biologically important in head and neck squamous cell carcinoma (HNSCC) ( xref )."
22664184
sparser
"Interestingly, when we defined DCC and UNC5C methylation at 1 % or more as a continuous variable (i.e., >1.0 % methylation as methylation-positive (methylated) and <1.0 % methylation as methylation-negative (unmethylated)), only UNC5C methylation was significantly associated with the presence of the cagA sequence in normal gastric mucosa (data not shown), suggesting that inflammatory processes associated with H. pylori infection causes aberrant methylation in UNC5C promoter CpGs but not in the DCC promoter."
26207151
sparser
"In conclusion, aberrant methylation of EDNRB, KIF1A, DCC and p16(INK4a) genes is a common event in Indian OSCC, suggesting that epigenetic alterations of these genes warrant validation in larger studies for their potential use as biomarkers."
20473870
sparser
"We found that among the cancers with complete loss of DCC expression, 5/7 cancers (71 %) demonstrated both DCC promoter methylation and 18q LOH."
26207151
sparser
"However, CD1A and DCC methylation had lower probabilities indicating that in the tumor microenvironment, some genes are stably methylated while others are not, presumably to promote the tumorigenic phenotype."
26566081
sparser
"EDNRB was methylated in 67 of 92 (72.8%) tumors, KIF1A was methylated in 66 of 92 (71.7%) tumors, p16 was methylated in 44 of 92 (47.8%) tumors and DCC was methylated in 54 of 92 (58.7%) tumors."
20473870