IndraLab

Statements

TSC1 binds TSC2 and RB1CC1. 23 / 23
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sparser
"We found that association of FIP200 or its segments N1-859 and N1-638 with the TSC1–TSC2 complex correlated with their ability to increase cell size, up-regulate S6K phosphorylation, and decrease TSC1–TSC complex formation."
16043512
sparser
"These results suggest that FIP200 interaction with the TSC1–TSC2 complex might not be required for FIP200 regulation of cell cycle progression."
16043512
sparser
"Together, these studies suggest that FIP200 interaction with the TSC1–TSC2 complex inhibits its function in the negative regulation of S6K activation through mTOR, which is responsible for the regulation of cell size control by FIP200."
16043512
sparser
"Thus, the identification of the interaction between FIP200 and the TSC1–TSC2 complex raises the possibility that this interaction may play a role in the regulation of cell cycle progression by FIP200."
16043512
sparser
"Similar studies indicated that the N1-859 segment of FIP200, but not the N1-638 segment, which does not bind to TSC1–TSC2, also increased the average cell size of the transfected 293T cells ( xref )."
16043512
sparser
"FIP200 interaction with the TSC1–TSC2 complex is not involved in FIP200 regulation of cell cycle progression."
16043512
sparser
"Therefore, we examined the possible effect of FIP200 on TSC1–TSC2 complex–mediated inhibition of S6K to determine the mechanisms by which FIP200 interaction with the TSC1–TSC2 complex regulated cell size."
16043512
sparser
"We found that association of FIP200 with the TSC1–TSC2 complex correlated with its ability to increase cell size and up-regulate S6 kinase phosphorylation but was not involved in the regulation of cell cycle progression."
16043512
sparser
"Here, we report identification of FIP200 interaction with the TSC1–TSC2 complex and show that this interaction leads to inhibition of TSC1–TSC2 complex function resulting in increased S6K activity and cell growth."
16043512
sparser
"We initially hypothesized that the interaction between FIP200 and the TSC1–TSC2 complex would play a role in FIP200-mediated cell cycle progression."
16043512
sparser
"In addition, the FIP200 N1-638 segment does not interact with the TSC1–TSC2 complex but still inhibits cell cycle progression."
16043512
sparser
"Although we cannot exclude completely the possible role of other as yet unidentified proteins that interact with FIP200, current evidence suggests that these effects are through FIP200 interaction with the TSC1–TSC2 complex."
16043512
bel
"We found that association of FIP200 with the TSC1-TSC2 complex correlated with its ability to increase cell size and up-regulate S6 kinase phosphorylation"
16043512
sparser
"Therefore, we tested whether FIP200 could associate with the TSC1–TSC2 complex in cells."
16043512
sparser
"Here, we identify an interaction between FIP200 and the TSC1–TSC2 complex through FIP200 binding to TSC1."
16043512
sparser
"In turn, FIP200 interacts with tuberous sclerosis complex 1 (TSC1), which induces the disruption of the TSC1-TSC2 complex, leading to mTORC1 activation [96]."
34360716
sparser
"Indeed, the FIP200 N1-859 fragment, which can interact with the TSC1–TSC2 complex ( xref A), showed similar activity as the full-length FIP200 in the inhibition of cell cycle progression as measured by BrdU incorporation (not depicted)."
16043512
sparser
"Together, these data suggest that interaction between FIP200 and the TSC1–TSC2 complex is not involved in FIP200-mediated cell cycle progression."
16043512
sparser
"Association of FIP200 with the TSC1–TSC2 complex."
16043512
sparser
"Together, these data suggest that FIP200 interaction with the TSC1–TSC2 complex is not required for FIP200 regulation of cell cycle progression."
16043512
sparser
"Consistent with these transfection studies, we could also detect the interaction of endogenous FIP200 with the endogenous TSC1–TSC2 complex in 293T cells ( xref E)."
16043512
sparser
"Therefore our identification of the interaction between FIP200 and the TSC1–TSC2 complex raises the interesting possibility that FIP200 may regulate cell size through interaction with the TSC1–TSC2 complex."
16043512
sparser
"However, the smaller segment of FIP200 (N1-638; xref A), which did not associate with the TSC1–TSC2 complex ( xref A), could also inhibit cell cycle progression ( xref )."
16043512