"Our findings show that RPS3 interacts with both p53 and MDM2."
"The results from our DNA pull-down assay offers the intriguing possibility that the presence of 8-oxodG in damaged DNA could act as staging position for the interaction of RPS3 with p53 and MDM2."
"What remains to be seen is how the interaction between RPS3, p53 or MDM2 affects genomic stability, especially at sites of 8-oxodG"
"We therefore tested whether the interaction between RPS3 and p53 or MDM2 intensified under oxidative stress."
"As a final means of establishing the interaction between RPS3 and p53-MDM2, we employed in situ PLA."
"We used confocal microscopy to test that the interaction between RPS3, MDM2, and p53 takes place at a single cell level."
"In an attempt to identify other novel proteins that interact with MDM2, we performed a co-precipitation of proteins bound to a biotinylated MDM2 peptide attached to streptavidin beads in which ribosomal protein S3 (RPS3) was one of the two proteins captured, and subsequently led to the discovery that RPS3 also interacts with p53 to protect it from MDM2-mediated ubiquitination."
"To understand at a greater depth the nature of the interaction between RPS3 and p53 or MDM2, we used the cis -imidazoline analog Nutlin-3, which is known to be a potent inhibitor of the MDM2 interaction with p53 [ xref ]."
"RPS3 interacts with MDM2 and p53."
"Our in vitro and in vivo data suggest that at least one biological endpoint of the interaction of RPS3 with p53 and MDM2 is to protect p53 from MDM2-mediated ubiquitination."
"As an additional means of confirming that RPS3’s interaction with p53 and MDM2 is legitimate, we took advantage of RPS3 mutant-constructs that are abrogated at the ERK1/2 phosphorylation site (T42)."
"Given that RPS3 interacts with both p53 and MDM2, we next explored how RPS3 might affect these proteins in an in vitro assay that measures the ubiquitination of p53 by MDM2."