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MTOR phosphorylates RPS6KB1. 496 / 508
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"Some studies suggest that mTOR can directly phosphorylate its effectors, such as S6K1 and 4E-BP1, while others claim that the mTOR dependent phosphorylation of S6K1 and 4E-BP1 is an indirect result of the inhibition of PP2A [XREF_BIBR; XREF_BIBR]."
18543252
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"Phosphorylation of S6K1 by mTOR in the heart is mediated by the mTOR complex 1 (mTORC1), which is sensitive to rapamycin."
22465089
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"42 However, inhibition of mTOR by some chemicals, such as rapa, will promote AKT phosphorylation by blocking the phosphorylation of insulin receptor substrate 1 and p70 ribosoma S6 kinase, XREF_BIBR, XREF_BIBR which are known to be a part of a negative feedback mechanism of PI3K-AKT signal transduction."
27253415
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"In mTORC1, mTOR binds to Raptor, mLST8 and PRAS40, and phosphorylates the translational factors S6K-1 and 4E-BP1 [XREF_BIBR], regulating different cell processes in response to nutrients and/or growth factors [XREF_BIBR]."
25840913
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"As previously reported in cancer cells (Mamane et al., 2006), Mtor could induce phosphorylation of p70S6K, Eif4ebp1 and cause their dissociation from Eif3 and Eif4e respectively, inducing the translat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29883638
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"When mTOR phosphorylates p70S6K, this kinase phosphorylates S6 ribosomal protein in return [XREF_BIBR, XREF_BIBR]."
23056046
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"Further downstream, mTOR phosphorylates p70 S6 kinase (T389), a regulator of Ribosomal Protein S6 (RPS6) responsible for mRNA translation, and has been proposed to signal via 14-3-3 (tyrosine 3-monooxygenase), a required protein in Raf signaling, to inhibit IRS-1 in a negative feedback loop xref ."
21980324
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"An inhibitor of the mammalian target of rapamycin (mTOR) blocked the stretch induced phosphorylation of p70S6K but did not affect the eEF2 activation."
20957453
rlimsp
"To activate the translational cascade, mTOR phosphorylates S6 kinase (S6K1), which is liberated from the eIF3-complex and mobilized for activation of its downstream targets."
26172298
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"Interestingly, the study has also found that autophagy activators decrease the cytotoxicity of fenofibrate, while autophagy inhibitors increase the fenofibrate-induced glioblastoma cytotoxicity including phosphorylation of AMPK and suppression of mTOR- dependent phosphorylation of p70S6K ( xref )."
33833983
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"MTOR is eventually activated and induces the phosphorylation of ribosomal protein S6 and its complex p70 S6 kinase (S6K) to promote ribosome biosynthesis, thereby promoting hyperactive HIF-1α translation [ xref ]."
| PMC
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"Following that, mTOR associates with Raptor (mTORC1 complex) to phosphorylate p70 S6K1, leading to increased cell proliferation [XREF_BIBR]."
25788964
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"Recent studies established that amino acids and insulin induce p70 S6 kinase (p70 (S6k)) phosphorylation by mTOR, involved in translational control of protein synthesis."
14610086
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"Numerous components involved in the initiation and elongation stages of translation are regulated by mTOR, a protein kinase that phosphorylates 4E-BP (eIF4E binding proteins) and the ribosomal protein p70 S6 kinase 1 (p70 S6K1)."
22227453
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"cAMP elevation has also been shown to inhibit IL-2-stimulated activation of PI3K and downstream, mammalian target of rapamycin dependent phosphorylation of p70 s6 kinase in T cells."
20515836
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"Akt activation leads to activation of the downstream mTOR and Raptor complex, which in turn phosphorylates p70 S6 kinase and 4E-BP1, leading to activation of cap dependent translation."
21241278
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"66 In response to PI3K/Akt signaling, mTOR rapidly phosphorylates both p70 s6k and 4EBP1, the latter leading to release of eIF4E, resulting in initiation of translation; these[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12819026
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"In human prostate cancer cells, constitutive expression of HIF-1alpha was found to require signal transduction via phosphatidylinositol-3-kinase (PI3K), AKT (protein kinase B), and the mammalian target of rapamycin (mTOR; also known as FKBP and rapamycin binding protein), a serine/threonine protein kinase that phosphorylates p70 ribosomal protein S6 kinase 1 (S6K1) and the eIF-4E binding protein 1 (4E-BP1), thereby stimulating protein synthesis."
22811423
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"MTOR then stimulates translation by phosphorylating p70 S6 kinase and, consequently, the 40 S ribosomal protein S6."
14982927
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"MTOR signaling components including mTOR phosphorylation and phosphorylation of S6K1 are elevated in aged muscle exposed to leg resistance exercise xref ."
22701343
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"MTOR complex 1 (mTORC1), an established druggable target against cancer, phosphorylates and controls its substrates p70 ribosomal protein S6 kinase 1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1) to promote protein translation as well as anabolic metabolism downstream of growth factor receptor activated PI3K-Akt signaling [XREF_BIBR, XREF_BIBR]."
30347859
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"Here we show that hydrogen peroxide (H 2 O 2), a major oxidant generated when oxidative stress occurs, induced apoptosis of neuronal cells (PC12 cells and primary murine neurons), by inhibiting the mammalian target of rapamycin (mTOR)-mediated phosphorylation of ribosomal p70 S6 kinase (S6K1) and eukaryotic initiation factor 4E (eIF4E) binding protein 1 (4E-BP1)."
20142804
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"In our hands, rictor-mTOR neither binds nor phosphorylates PKCα, but given the complex mechanisms involved in the phosphorylation of S6K1 by raptor-mTOR, these negative findings must be taken with cau[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15268862
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"MTOR also allows translation initiation by phosphorylating and activating the kinase p70S6K which in turn phosphorylates the 40S ribosomal subunit S6 protein 30."
23988451
sparser
"Phosphorylation of S6K1 by mTOR in the heart is mediated by the mTOR complex 1 (mTORC1), which is sensitive to rapamycin."
22465089
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"A recent model proposed by the Blenis laboratory suggests that S6K1 is phosphorylated in the pre and initiation complex by mTOR leading to dissociation and full activation of S6K1 by PDK1."
16953237
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"This implies that there are distinct pathways of p70 S6 kinase phosphorylation by mTOR and a PI3-kinase."
10376934
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"The principal regulatory input for S6K1 is believed to be provided by mTOR signaling pathway wherein mTOR kinase directly phosphorylates S6K1 at its hydrophobic motif to trigger a second phosphorylation at its activation loop for full activation [XREF_BIBR, XREF_BIBR]."
24605337
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"The reduction in 4E-BP1 phosphorylation was associated with a reduction in the abundance of Raptor and mTOR proteins, Raptor associated mTOR, reduced phosphorylation of the downstream protein p70S6 kinase, and attenuated incorporation of [(14) C] phenylalanine into protein."
17510058
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"On the basis of high-throughput bioavailability studies of brain bioavailable metabolites after dietary BDPP treatment, we found that select polyphenol metabolites [e.g., cyanidin-3 '- O-glucoside and 3-(3 '-hydroxyphenyl) propionic acid] were able to rescue mTOR and p70S6K phosphorylation in primary cortico-hippocampal neuronal cultures, as well as rescue 4E-BP1 phosphorylation in response to treatment with 4EGI-1, a specific inhibitor of eIF4E-eIF4G interaction."
29702026
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"Moreover, for S6K1 activation, mTOR can directly phosphorylate S371 in vitro, and this event modulates T389 phosphorylation by mTOR [XREF_BIBR, XREF_BIBR]."
20491627
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"It has been shown that rapamycin inhibits mTOR-mediated phosphorylation of S6K1 and 4E-BP1 by activation of PP2A [35]."
26118661
sparser
"MTOR phosphorylates ribosomal p70S6 kinase 1 (p70S6K) at its hydrophobic motif site, T389; in turn, the phosphorylated p70S6K phosphorylates its substrate, the S6 ribosomal protein at S235/236."
31409554
rlimsp
"Infusion of EAA+Glc reduced phosphorylation of the ISR target, eukaryotic initiation factor(eIF) 2, in mammary tissue and increased phosphorylation of the mTOR targets, ribosomal S6 kinase 1 (S6K1) and S6."
20032484
sparser
"The activated mTOR could phosphorylate p70S6K, and the activated p-p70S6K could further phosphorylate 4EBP1."
33539323
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"Studies show that mTOR phosphorylation of p70S6K1 increases its kinase activity; and that phosphorylation of 4E-BP1 results in the dissociation of 4E-BP1 from eIF4E and the activation of cap dependent translational initiation XREF_BIBR."
24466059
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"Here, we demonstrate that the raptor and mTOR complex phosphorylates the rapamycin sensitive forms of S6K1, while the distinct rictor and mTOR complex phosphorylates the rapamycin resistant mutants of S6K1."
15809305
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"MTOR phosphorylates Thr‐389 of S6K1 in the regulatory T loop, an event required for S6K1 activation."
28242756
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"Alternative translation of C/EBPbeta towards the small isoform LIP depends on mTOR which activates eIF4E [XREF_BIBR] and phosphorylates S6K1 [XREF_BIBR]."
26646662
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"PTEN inhibits the activation of Akt by PI3K, mTOR phosphorylates p70S6K1 (S6K1) and 4E-BP1 leading to activation of pathways involved in cell growth and survival."
33912467
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"Activated mTOR induces phosphorylation of p70S6K followed by p70S6K induced phosphorylation of S6 and the eukaryotic translation initiation factor 4B (eIF-4B), which promotes protein translation (Wang[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22264868
sparser
"RAFT1 phosphorylates p70(S6k) much more effectively than 4E-BP1, and the phosphorylation sites on the two proteins show little homology."
9465032
sparser
"Mammalian target of rapamycin (mTOR) kinase phosphorylates p70 S6 kinase (p70S6K), which regulates the phosphorylation of S6 ribosomal protein (S6RP), the functional site of the 40S ribosomal subunit."
27632912
sparser
"In response to mitogen stimulation, mTOR phosphorylates and activates S6K1, which in turn phosphorylates the 40S ribosomal protein S6, leading to the enhancement of translation of mRNAs with a 5′-terminal oligopyrimidine, including mRNAs that encode for ribosomal proteins and elongation factor-1."
19437488
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"Additionally, phosphorylation of p70 S6 kinase (S6K) by mTOR promotes the phosphorylation of its substrate, the ribosomal protein S6, and induces protein translation XREF_BIBR (XREF_FIG a)."
26579469
rlimsp
"After processing a cell proliferative up-stream signal mediated by the PI3K/Akt pathway, mTOR phosphorylates and activates p70S6K."
22460505
sparser
"MTOR can also phosphorylate p70 ribosomal S6 kinase (p70S6K)."
19631745
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"XREF_BIBR mTOR activation by growth factors leads to phosphorylation of p70S6K and 4E-BP1 involved in cellular translation."
27995152
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"For example, mTOR phosphorylation of p70S6 kinase leads to downstream phosphorylation of the 40S ribosomal protein S6, resulting in increased translation from mRNAs that contain the 5 ' -terminal oligopyrimidine tract, such as those for the elongation factor-1alpha [XREF_BIBR]."
23369283
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"Mammalian TOR, mTOR (also known as RAFT1 or FRAP), phosphorylates at least two regulators of protein synthesis : S6K1 (formerly called p70 ribosomal S6 kinase) and an inhibitor of translation initiati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12150925
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"HNSCC is characterized by persistent activation of the Akt and mTOR pathway that leads to phosphorylation of p70 S6 kinase and 4E-BP1."
19625495
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"SESN2 inhibits mTOR-dependent phosphorylation of p70S6K and 4E-BP1, and knockdown of SESN2 resulted in the activation of mTOR signaling indicating that the important role of SESN2 on mTOR inhibition."
30936621
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"MTOR phosphorylates the eukaryotic initiation factor 4E-binding protein (4E-BP) and the p70S6 kinase1 (S6K1)."
34527050
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"Inhibiting mTOR not only blocks the phosphorylation of S6K1 and 4E-BP1, but also induces autophagy [XREF_BIBR - XREF_BIBR]."
26339598
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"Mammalian TOR, mTOR (also known as RAFT1 or FRAP), phosphorylates at least two regulators of protein synthesis : S6K1 (formerly called p70 ribosomal S6 kinase) and an inhibitor of translation initiati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12150925
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"This portion of the p70S6K protein is phosphorylated by mTOR in both TORC1 and TORC2 complexes."
24740573
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"To further characterize how rapamycin treatment modulates ovarian follicle differentiation, we performed immunohistochemical analysis on the localization of mTOR and phosphorylated p70S6K in ovaries."
23566837
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"Rotenone dramatically inhibited mTOR mediated phosphorylation of S6K1 and 4E-BP1, which was also attenuated by CAT in the neuronal cells."
25304210
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"Thus, our data indicate that mTOR inhibition increases lapatinib sensitivity in association with reduced Akt and possibly reduced p70S6K phosphorylation."
22043997
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"One possible link is eIF3; recent work XREF_BIBR has suggested that a conserved TOS motif in eIF3f allows it to interact with the mTOR and raptor complex, which phosphorylates S6K1 XREF_BIBR and regulates downstream effectors of mTOR and cap dependent translation initiation."
25486193
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"It has also been claimed that mTOR (mammalian target of rapamycin) can phosphorylate the PDK2 site of p70 S6 kinase [39]."
10226025
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"Moreover, phosphorylation of the phosphatase PP2A by mTOR prevents the dephosphorylation of 4E-BP1 and S6K1 (Peterson et al., 1999)."
18439897
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"Pretreatment of cells with wortmannin and LY-294002, inhibitors of PI3K, or rapamycin, an inhibitor of the mammalian target of rapamycin kinase and p70S6K, diminished the ANG IV mediated activation of PDK-1 and PKB-alpha as well as phosphorylation of p70S6K."
12225947
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"MTOR phosphorylates at least two substrates, p70S6 kinase and the translational repressor eukaryotic initiation factor 4E binding protein 1 (4E-BP1) [XREF_BIBR]."
18759130
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"MTOR activation results in phosphorylation of effector molecule p70S6K and S6 ribosomal protein, which subsequently leads to mTOR dependent gene transcription that regulates cell growth, protein synthesis, and metabolism."
20576128
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"To determine whether the nuclear accumulation of ribosomal proteins is dependent on mTOR, we analyzed abundance of the ribosomal proteins S3, S6, and L26 [ xref ] in both nuclear (SENF and RNF) fractions following a potent inhibition of mTOR by pp242 [ xref ] as detected by the mTOR-dependent phosphorylation of S6K1 and Akt (Fig. xref , the lower panel)."
25294810
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"In addition, mTOR phosphorylates and activates p70S6K at threonine 389 and is a marker of mTOR activity [ xref ]."
23244622
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"Raptor is a 150kDa mTOR binding protein that also binds S6K1, serves as a scaffold protein of mTOR and facilitates mTOR phosphorylation of S6K1 XREF_BIBR XREF_BIBR XREF_BIBR."
23532117
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"Bupivacaine induced loss of S6K1 phosphorylation at putative mTOR site and at the activation loop as a consequence, without any perceptible change in mTOR kinase activity or expression observed herein, substantiates the later possibility even further."
24605337
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"In addition, mTOR might promote mRNA translation by phosphorylating S6K1 and then p70S6K to promote the adhesion of ribosomes to the endoplasmic reticulum, thereby preventing the formation of autophagic membranes and inhibiting autophagy activity [XREF_BIBR, XREF_BIBR]."
30832706
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"Moreover, for S6K1 activation, mTOR can directly phosphorylates Ser371 in vitro, and this event modulates Thr389 phosphorylation by mTOR."
20528734
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"Phosphorylation of p70S6K by activated mTOR leads to the degradation of insulin like growth factor receptor substrate 1, and consequently to suppression of PI3K and Akt signaling."
20030877
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"MTOR phosphorylates p70 ribosomal S6 kinase (p70S6K) and eukaryotic initiation factor 4E binding protein 1 (4E-BP1)."
19351820
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"Lv Xin et al. found through an experiment that the high mTOR expression in the proliferative phase of hemangioma might lead to the activation and phosphorylation of p70S6K-alpha and thus promote the rapid proliferation of vascular endothelial cells of hemangioma."
33575332
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"MTOR specific phosphorylation of p70S6 kinase and 4E-BP, and expression of Raptor are all enhanced during M-phase."
21750711
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"Activation of mTOR by mitogens and growth factors leads to phosphorylation of S6K1 by mTOR at the hydrophobic motif site T389 within the catalytic domain, a critical event in S6K1 activation."
19302577
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"To identify additional mTORC1 binding elements of PRAS40, we first tested whether the PRAS40 segment reported to be necessary for inhibition 14 (PRAS40 114-256) can also inhibit mTOR DeltaN -mLST8 phosphorylating S6K1 367-404."
29236692
rlimsp
"A point mutation of the TOS motif also eliminates all in vitro mTOR-catalyzed 4E-BP1 phosphorylation and abolishes the raptor-dependent component of mTOR-catalyzed p70S6k phosphorylation in vitro."
12604610
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"The activation of mTOR is supported by increased phosphorylation of upstream proteins including ERK and Akt, and mTOR then phosphorylates and activates the downstream protein p70S6K, which inhibits eEF2K, thereby decreasing the phosphorylation of eEF2 and effectively inhibiting eukaryotic initiation factor 4E (eIF4E)."
30089868
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"More studies demonstrated that enhanced phosphorylation of S6K1 and rpS6 signalling by TSC and mTOR could promote the follicular activation 7, 42."
28881413
trips
"Immunopurified mammalian target of rapamycin phosphorylates and activates p70 S6 kinase alpha in vitro."
10567431
sparser
"MTOR complex 1 (mTORC1) phosphorylates S6K1, while mTORC2 phosphorylates Akt."
18411301
sparser
"MTOR phosphorylates p70S6K, which in turn phosphorylates the S6 protein in the 40S small ribosomal subunit."
12935893
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"ROS triggered PI3K activation can transduce oncogenic AKT-mTOR signaling; mTOR can increase the phosphorylation of both eukaryotic initiation factor 4E binding protein1 (4EBP-1) and S6K1, leading to the translation and expression of mRNAs encoding several major anti-apoptotic proteins including XIAP, c-IAP1, Bcl-XL, and BCl-2 [XREF_BIBR]."
28250388
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"Complete phosphorylation of P70S6K1 by mTOR requires phosphorylation by PDK1 and has recently been suggested to be proceed that of mTOR (Keshwani etal."
26010896
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"4E-BP1 and S6K1 are phosphorylated by mammalian target of rapamycin complex 1 (mTORC1) which regulates the binding of eIF4E to the mRNA 5’cap through phosphorylation of 4E-BP1 and stimulates protein synthesis through the phosphorylation of S6K1 xref xref xref xref ."
28051178
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"Results from published studies showed that mTOR-dependent phosphorylation of p-S6K is not linearly correlated with 4EBP1 phosphorylation xref xref , which could partly explain the lack of prognostic value of p-S6K observed in this study."
27026382
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"The mTOR complex phosphorylates S6K1 and 4E-BP1."
28053551
sparser
"Activated mTOR phosphorylates p70S6K and this leads to increased ribosomal biogenesis ( xref ; xref )."
18593953
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"MTOR phosphorylates p70S6 kinase (p70S6K) at several residues, resulting in increased kinase activity ( xref )."
24662921
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"Phosphorylation of S6K1 by mTOR enhances the translational capacity by acting on translation initiation complex assembly."
17646396
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"Based on these studies, one could hypothesize that the activation of mTOR signaling increases the phosphorylation of p70S6K and 4E-BP-1, thereby promoting the initiation of protein translation for synaptic protein synthesis."
26522512
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"MTOR associates with Raptor (mTORC1 complex) to phosphorylate the p70 S6 kinase (S6K), which in turn phosphorylates the S6 ribosomal protein (S6), as well as the eIF4E inhibitor, 4E-BP1, leading to protein translation."
20703254
sparser
"The phosphorylation of S6K1 by mTOR is confined to a single residue (T389)( xref ), and this phosphorylation was eliminated by pemetrexed ( xref ), whereas four residues on 4EBP1 are phosphorylated by mTOR ( xref ); the broader migration pattern seen in pemetrexed-treated cells with the pan-4EBP1 antibody ( xref ) suggests that several of these phosphorylation sites are affected by drug."
19549896
sparser
"MTOR phosphorylates 4E-BPs ( e ukaryotic I nitiation F actor 4E-B inding P roteins, which are inhibitors of eIF4E) and S6Ks (ribosomal protein S6 Kinases) to promote translation initiation ( xref )."
25466251
sparser
"Additionally, phosphorylation of p70 S6 kinase (S6K) by mTOR promotes the phosphorylation of its substrate, the ribosomal protein S6, and induces protein translation xref ( xref a)."
26579469
sparser
"Akt can activate its downstream serine/threonine kinase mammalian target of rapamycin (mTOR), which then phosphorylates p70 ribosomal protein kinase (p70S6K) and 4E-binding protein 1 (4E-BP1), leading to initiation of protein synthesis."
31780937
rlimsp
"MTOR could phosphorylate and activate p70s6k through interacting with it [10]."
25738361
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"P70S6K phosphorylation and protein synthesis can be blocked by inhibiting mTOR, which is a key translational regulator in both neuronal and non neuronal tissues."
20029971
sparser
"P70S6K is phosphorylated directly by mTOR( xref ) and can be used as an indicator of mTOR activity."
32467173
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"Overexpression or overactivation of mTOR may strengthen the signals passed down by mTOR signaling pathway, which will cause over-phosphorylation of the downstream molecules p70S6K and 4E-BP1."
23173671
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"Moreover, mTOR inhibition by rapamycin and PP242 (inhibitor of mTORC1 and mTORC2) significantly decreased S6K1 phosphorylation and subsequently diminished this PAIP1-eIF3 interaction [111, 118]."
28083147
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"In the mTORC1 complex, mTOR phosphorylates p70S6K (70 kDa ribosomal protein S6 kinase) and 4E-BP1 (eIF4E binding protein), leading directly to increased translation and synthesis of cell cycle regulating and ribosomal proteins [XREF_BIBR, XREF_BIBR]."
22865098
sparser
"In the mTORC1 complex, mTOR phosphorylates p70S6K (70 kDa ribosomal protein S6 kinase) and 4E-BP1 (eIF4E-binding protein), leading directly to increased translation and synthesis of cell cycle regulating and ribosomal proteins [ xref , xref ]."
22865098
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"AZD8055 (XREF_FIG) is a prototypic, orally-available, ATP-competitive mTOR kinase inhibitor, that suppresses phosphorylation of the mTORC1 substrates p70S6K and eukaryotic initiation factor 4E binding protein 1 (4E-BP1), as well as phosphorylation of the mTORC2 substrate AKT (S473)."
25307040
sparser
"Active mTOR in turn phosphorylates both the p70S6 kinase (S6K), which promotes translation elongation and ribosome biogenesis, and the eIF4E binding protein (4E-BP)."
24351799
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"Moreover, mTOR inhibition by rapamycin and PP242 (inhibitor of mTORC1 and mTORC2) significantly decreased S6K1 phosphorylation and subsequently diminished this PAIP1-eIF3 interaction [XREF_BIBR, XREF_BIBR]."
28083147
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"Curcumin inhibits mTOR mediated phosphorylation of S6K1 and 4E-BP1 in a PP2A independent manner."
19176385
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"Autophagy is negatively regulated by mTOR, which phosphorylates p70S6K, in turn dephosphorylating ULK1, a component of the autophagy initiation complex [XREF_BIBR]."
28610619
reach
"Since mTor phosphorylates and activates p70S6 kinase, inhibition of mTor by rapamycin can result in reduced p70S6 kinase activity [28]."
15369799
reach
"MTOR complex phosphorylates S6K1 and regulates ribosomal protein synthesis."
19501448
reach
"4E-BP is phosphorylated by mTOR and S6 is a substrate of S6 kinase 1 (S6K1), which is also phosphorylated by mTOR."
24126619
rlimsp
"The PI3K/AKT signaling pathway activates mTOR, which in turn directly phosphorylates ribosome protein S6 kinase 1 (S6K) and eIF4E-binding protein 1, both of which are important in control of protein translation initiation (19,20)."
26323436
sparser
"We also found that LPAR5‐specific antagonist TC LPA5 4, PI3K inhibitor wortmannin, or mTOR inhibitor rapamycin pretreatment abrogated phosphorylation of Akt and p70S6K1 stimulated by LPA in CGTH‐W3 and TPC‐1 cells."
33540491
sparser
"Activated mTOR phosphorylates p70S6K, one of the most studied substrates of mTOR."
30420897
reach
"In addition, mTOR activation and phosphorylation of S6K1 and 4E-BP1 in rat hippocampus was observed during a spatial learning paradigm."
27378854
sparser
"MTOR phosphorylates p70 ribosomal S6 kinase (p70S6 kinase) that regulates translation of proteins involved in cellular proliferation and formation."
20576128
reach
"The inhibition of mTOR decreases phosphorylation and activation of p70S6K and 4EBP1, which results in the inhibition of translation of critical mRNA involved in tumorigenesis [XREF_BIBR, XREF_BIBR]."
24818169
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"Contents of Akt, phosphorylated Akt (pAkt), Erk, phosphorylated Erk (pErk), mammalian target of rapamycin (mTOR), phosphorylated p70S6 kinase and actin were analyzed by immunoblotting of cell lysates."
23891792
sparser
"Activated mTOR phosphorylates at least two targets, p70S6K and 4E-BP1, which in turn activates p70S6K and inhibits 4E-BP1."
24312355
rlimsp
"Three of these sites are highly conserved among AGC kinases (cAMP dependent Protein Kinase, cGMP dependent Protein Kinase, and Protein Kinase C subfamily): the activation loop in the kinase domain, and two C-terminal sites, the turn motif and the hydrophobic motif. The common dogma has been that phosphorylation of the hydrophobic motif primes S6K1 for the phosphorylation at the activation loop by phosphoinositide-dependent protein kinase 1 (PDK1). Here, we show that the turn motif is, in fact, phosphorylated first, the activation loop second, and the hydrophobic motif is third. Specifically, biochemical analyses of a construct of S6K1 lacking the C-terminal autoinhibitory domain as well as full-length S6K1, reveals that S6K1 is constitutively phosphorylated at the turn motif when expressed in insect cells and becomes phosphorylated in vitro by purified PDK1 at the activation loop. Only the species phosphorylated at the activation loop by PDK1 gets phosphorylated at the hydrophobic motif by mammalian target of rapamycin (mTOR) in vitro."
21561857
sparser
"MTOR in turn phosphorylates S6K1 and 4EBP1 to directly alter protein translation."
19295495
sparser
"Phosphorylation of p70S6K by mTOR leads to phosphorylation of insulin receptor substrate-1 (IRS-1) and its degradation via the proteasome [ xref ]."
28807816
sparser
"In contrast, CRYAB knockdown did not profoundly alter the levels of phosphorylated mTOR and phosphorylated p70S6K (Fig. xref f)."
30675347
reach
"MTOR activation enhances protein translation by phosphorylating its targets p70S6K and 4E-BP1 and inhibits autophagy [9]."
25476892
reach
"Activation of mTOR by FGF9 induces p70 ribosomal S6 kinase (S6K1) phosphorylation, cyclin expression, and cell proliferation, which are independent of phosphatidylinositol 3-kinase and Akt."
15760907
sparser
"MTOR directly phosphorylates the ribosomal p70S6 kinase (p70S6K) and the initiation factor 4E-binding protein 1 (4E-BP1) to initiate translation of distinct mRNAs ()."
18848473
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"PRL-activated mTOR may phosphorylate p70S6K and 4E-BP1 by restraining PP2A."
16899564
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"On stimulation by insulin, other growth factors, cytokines, or nutrients, mTOR phosphorylates at least two regulators of translational protein synthesis : S6K1 and 4E-BP."
17717968
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"In primary human AML blasts (AML-PB001F), Tim-3 agonistic antibody as well as galectin-9 (one of the natural ligands of Tim-3) induced activation of the mTOR pathway (by mTOR dependent phosphorylation of p70 S6 kinase 1 (p70 S6K1) and eIF4E binding protein-1 (eIF4E-BP1))."
26413815
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"MTOR activation leads to phosphorylation of S6K1 and 4E-BP to regulate protein synthesis, cell growth, and metabolism, and to cell survival via phosphorylating PKB on Ser473 [110]."
32640596
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"In Vgamma2Vdelta2 T cells, mTOR phosphorylates the S6K1 and eIF4EBP1 signaling intermediates after antigen stimulation."
21107834
sparser
"Activated mTOR phosphorylates p70S6K and this leads to increased ribosomal biogenesis ( xref ; xref )."
19588523
reach
"Both Raf/MEK/ERK and Akt and mTOR pathways phosphorylate p70S6K and then rpS6, increasing protein translation."
26459180
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"Expression of DN-epsilonPKC abolished ET-1-stimulated mTOR and S6K1 phosphorylation, but not insulin stimulated S6K1 phosphorylation."
19168855
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"In summary, we have identified that oxidative stress by H 2 O 2 inhibits mTOR mediated phosphorylation of S6K1 and 4E-BP1, leading to apoptosis of neuronal cells."
20142804
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"The results indicate mTOR independent phosphorylation of S6K1 and 4E-BP1 and suggest MEK/ERK/RSK1 dependent phosphorylation of eIF4B during skeletal muscle contraction."
23707523
sparser
"It is known that mTOR phosphorylates p70S6K followed by p70S6K-induced phosphorylation of eIF4B and S6 ( xref ) ( xref )."
22889863
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"Inactive eIF3 is bound to S6K1, while activated mTOR and Raptor binds to eIF3 and phosphorylates S6K1 causing its dissociation from eIF3; then, phosphorylated S6K1 phosphorylates several targets, such as eIF4B, thus implementing a translational control."
34395258
reach
"In addition, mTOR might promote mRNA translation by phosphorylating S6K1 and then p70S6K to promote the adhesion of ribosomes to the endoplasmic reticulum, thereby preventing the formation of autophagic membranes and inhibiting autophagy activity [XREF_BIBR, XREF_BIBR]."
30832706
reach
"Activated PI3K and Akt may positively regulate mTOR, which further causes phosphorylation of ribosomal p70 S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E (eIF4E) binding protein 1 (4E-BP1), the two best characterized downstream effector molecules of mTOR [XREF_BIBR, XREF_BIBR]."
27235588
sparser
"The mTOR complex phosphorylates S6K1 and 4E-BP1."
28053551
sparser
"Further, immobilization results in an attenuated response to leucine, shown through reduced phosphorylation of P70S6K. Complete phosphorylation of P70S6K1 by mTOR requires phosphorylation by PDK1 and has recently been suggested to be proceed that of mTOR (Keshwani et al . xref , Kelleher et al . xref ), yet the order in which these occur is still up for debate [discusses in Magnuson et al . ( xref )]."
26010896
sparser
"MTOR phosphorylates and activates S6K1, which in turn phosphorylates IRS-1 causing its internalization ( Um et al., 2004 )."
29729422
reach
"MTOR phosphorylates p70S6K, which in turn phosphorylates the S6 protein in the 40S small ribosomal subunit."
12935893
reach
"For example, mTOR signaling through the tuberous sclerosis complex (TSC1, hamartin / TSC2, tuberin) can inactivate IRS and phosphorylate p70S6K to block IRS1 activity by direct phosphorylation [XREF_BIBR]."
22545721
sparser
"MTOR phosphorylates the p70 ribosomal S6 kinase (p70S6K) leading to phosphorylation of the ribosomal protein S6, which stimulates mRNA translation rates. xref Antibodies that recognize phosphorylated S6 (Ser240/244) are widely accepted functional readouts of mTOR activity. xref Therefore, to establish whether RGC dendritic retraction correlates with changes in mTOR activity in these neurons, we examined phospho-S6 (Ser240/244) expression in intact and axotomized retinas."
25257176
sparser
"It has been shown that SIRT1 inhibition induces the acetylation of S6K1 and inhibits the mTOR-dependent phosphorylation of S6K1, suggesting an interaction between SIRT1 and mTOR [ xref ] ( xref )."
32370170
reach
"Loss of mTOR signaling prevents phosphorylation of both p70S6K and 4EBP1 to lead to apoptosis XREF_BIBR."
23029019
reach
"MTOR phosphorylation led to the phosphorylation of p70S6K, mTOR dependent event, that was abrogated by the mTOR specific inhibitor rapamycin."
26053099
reach
"Anabolic pathways that promote fiber hypertrophy result in activation via phosphorylation of protein kinase B (Akt) and mechanistic target of rapamycin (mTOR), which leads to phosphorylation and activation of p70 S6 kinase (p70S6K) that in turn phosphorylates the ribosomal protein S6 [33]."
32019272
sparser
"Phosphorylation of p70S6K by mTOR is down-regulated in response to decreased amino acid availability and rapamycin treatment."
23861877
reach
"Inhibition of mTOR by microinjection of rapamycin locally into the IC reduced expression of eEF1A and post-synaptic density protein 95 (PSD-95), reduced S6K1 phosphorylation, and increased eEF2 phosphorylation; at the same time, this inhibition of mTOR in the IC attenuated long-term taste memory as analyzed by LI-CTA paradigm [XREF_BIBR]."
22792024
reach
"MTOR phosphorylates and activates S6K1, which in turn phosphorylates IRS-1 causing its internalization (Um et al., 2004)."
29729422
sparser
"Here, we show that RAFT1 directly phosphorylates p70(S6k), 4E-BP1, and 4E-BP2 and that serum stimulates RAFT1 kinase activity with kinetics similar to those of p70(S6k) and 4E-BP1 phosphorylation."
9465032
reach
"Activated mTOR increases protein translation through the phosphorylation of 4EBP1 and p70S6K."
29844867
reach
"It is unlikely, that this effect is related to the mTOR dependent phosphorylation of S6K1 and rpS6, because similar dephosphorylation of both proteins occurs by incubation of cells with the mTOR kinase inhibitor pp242."
25294810
reach
"MTOR phosphorylates p70S6K and creates a docking site for PDK1, which then phosphorylates Thr229 of p70S6K resulting in its activation."
24604594
sparser
"Phosphorylation of S6K1 by mTOR enhances the translational capacity by acting on translation initiation complex assembly ( xref ; xref )."
17646396
sparser
"The mTOR complex (composed on mTOR, raptor, and GβL) phosphorylates 4E-BP1 (a protein translation initiation inhibitor) and p70S6 kinase (which promotes protein translation)."
26424649
sparser
"The phosphorylation of p70S6K and 4EBP1 by mTOR and the phosphorylation downstream of ribosomal protein S6 and EIF-4B stimulate translational initiation and contribute to cell growth."
26394157
reach
"It has been described that mTOR mediates phosphorylation of S6K1 and 4E-BP1 by inhibiting PP2A [35]."
26118661
sparser
"Overexpression of DGKζ, but not DGKα, led to increased mTOR complex 1-dependent phosphorylation of S6K1 both in the presence and absence of serum, suggesting that DGKζ-derived PA may activate mTOR complex 1."
23847619
sparser
"Activated mTOR phosphorylates its targets S6K1 and 4E-BP1 ( xref )."
19414743
sparser
"Because mTOR phosphorylates S6K1 ( xref ), an event that is critical for maximal activation of the kinase, this finding suggests that portal AA delivery increases mTORC1 signalling in liver to a greater extent than peripheral delivery."
18842785
rlimsp
"Recently, raptor, a 150-kDa TOR-binding protein that contains a carboxyl-terminal WD-repeat domain, was discovered as a scaffold for the mTOR-catalyzed phosphorylation of 4E-BP1 and for the mTOR-mediated phosphorylation and activation of p70alpha."
14560963
reach
"MTOR dependent phosphorylation of S6K1 causes S6K1 activation, whereas mTOR dependent phosphorylation of 4E-BP1 leads to its dissociation from the initiation factor eIF4E, thereby enabling eIF4E derepression."
20145209
reach
"One potential mechanism through which the mutations in mTOR (XREF_FIG) might increase S6K1 and Akt1 phosphorylation is by altering mTORC1 or mTORC2 assembly."
24631838
reach
"To oversee the cap dependent protein translation upon activation, mTOR phosphorylates its two major downstream targets, p70S6K and 4EBP1 to promote cell growth, cell proliferation and cell survival."
21307646
sparser
"In order to further define the mechanism of action, we assessed the influence of AZM on the activity of recombinant mTOR in vitro using phosphorylation of a recombinant p70S6K-GST fusion protein as readout."
25500904
reach
"MTOR complex 1 (mTORC1), consisting of Raptor, GbetaL, PRAS40, and DEPTOR, phosphorylates 4E-BP1 and p70S6K and thus stimulates protein synthesis [XREF_BIBR]."
29854093
reach
"MTOR phosphorylates both p70S6K and eukaryotic initiation factor 4E binding protein (4E-BP1) via independent pathways."
26894018
sparser
"Activated mTOR phosphorylates and activates the protein kinase p70S6K. We therefore evaluated the protein abundance of phosphorylated and total mTOR ( xref , respectively) and p70S6K ( xref , respectively)."
18997818
sparser
"Supporting this, a similar mechanism was previously shown to operate in mammals, where activated mTOR phosphorylates eIF3-bound S6K1 in PICs, which triggers S6K1 activation and its subsequent dissociation from PICs (Holz et al. xref )."
29281028
sparser
"In primary human AML blasts (AML-PB001F), Tim-3 agonistic antibody as well as galectin-9 (one of the natural ligands of Tim-3) induced activation of the mTOR pathway (by mTOR-dependent phosphorylation of p70 S6 kinase 1 (p70 S6K1) and eIF4E-binding protein-1 (eIF4E-BP1))."
26413815
reach
"Similarly, inhibition of mTOR blocked p70S6K and PAK1 phosphorylation and actin remodeling."
23316499
sparser
"While T389 S6K1 can be efficiently phosphorylated in vitro by mTOR in the absence of raptor, T37/T46 4E-BP1 phosphorylation requires raptor( xref ; xref )."
21816276
sparser
"MTOR phosphorylates and activates S6K1, which in turn phosphorylates the IRS-1 causing its internalization ( xref )."
29729422
sparser
"MTOR phosphorylates S6K1 and 4E-BP1, and the most downstream effector, eIF4E. In this study, eIF4E levels were increased in 5 everolimus-resistant SCLC cell lines and in SBC5 R10 cells during everolimus therapy."
25884680
sparser
"Mammalian target of rapamycin (mTOR) is a master regulator of autophagy and phosphorylates p70S6K. Akt phosphorylates and thereby activates mTOR to inhibit autophagy, whereas AMP-activated protein kinase (AMPK), which is a key energy sensor and that regulates cellular metabolism to maintain energy homeostasis, promotes autophagy xref ."
30538224
reach
"MTOR directly phosphorylates and activates p70S6K but knockdown of P5CS did not alter p70S6K phosphorylation (XREF_SUPPLEMENTARY)."
26082174
reach
"The first of these proteins is p70S6K, which is phosphorylated by mTOR and consequently activated."
19231960
sparser
"It has also been claimed that mTOR (mammalian target of rapamycin) can phosphorylate the PDK2 site of p70 S6 kinase ."
10226025
reach
"The rapamycin dose most effective in decreasing lytic replication in B cells, without impairing cell growth (5 nM), was also very effective in inhibiting the phosphorylation of p70S6K by mTOR in Raji cells."
24917448
reach
"Here we show that hydrogen peroxide (H 2 O 2), a major oxidant generated when oxidative stress occurs, induced apoptosis of neuronal cells (PC12 cells and primary murine neurons), by inhibiting the mammalian target of rapamycin (mTOR)-mediated phosphorylation of ribosomal p70 S6 kinase (S6K1) and eukaryotic initiation factor 4E (eIF4E) binding protein 1 (4E-BP1)."
20142804
rlimsp
"MTOR-catalyzed p70alpha phosphorylation in vitro is accompanied by a substantial restoration in p70alpha kinase activity toward its physiologic substrate, the 40 S ribosomal protein S6."
10567431
reach
"This is supported by recent evidence showing that serum stimulated increases in TCTP are inhibited to a greater extent by mTOR kinase inhibitors (e.g. PP242 and AZD8055), which inhibit both p70 S6K1 and 4E-BP1 phosphorylation, than by rapamycin [XREF_BIBR]."
27813490
reach
"AKT subsequently activates many downstream targets for cell growth and survival, including the rapamycin sensitive mTOR complex 1 (mTORC1), which then phosphorylates p70S6K and translation initiation [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21074248
reach
"Similarly, phosphorylation of p70 S6 kinase by mTOR has been reported (Burnett et al., 1998)."
10580846
sparser
"MTOR phosphorylates and activates p70 S6 kinase, and also inhibits eukaryotic translation initiation factor 4E binding protein, resulting in enhanced protein synthesis and cell proliferation."
31312183
reach
"Specifically, activation of mTOR by insulin causes phosphorylation of ribosomal protein S6 kinase 1 (S6K1), which promotes mRNA translation and protein synthesis, and leads to down-regulation of insul[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23932046
reach
"Inhibition of PI3K or mTOR and p70S6K by wortmannin and rapamycin, respectively, increased apoptosis and inhibited phosphorylation of Akt and p70S6K induced by oxidative stress XREF_BIBR."
23118851
sparser
"M-TOR binds with Raptor and phosphorylates p70S6 kinase, a protein involved in numerous cell signalling pathways."
22863900
sparser
"MTOR directly phosphorylates and activates p70S6K ( xref ) but knockdown of P5CS did not alter p70S6K phosphorylation ( xref )."
26082174
rlimsp
"The phosphorylation of p70S6K and 4EBP1 by mTOR and the phosphorylation downstream of ribosomal protein S6 and EIF-4B stimulate translational initiation and contribute to cell growth."
26394157
sparser
"Here, activated mTOR phosphorylates its substrate S6K1, a kinase involved in the control of protein translation."
32604886
reach
"In skeletal muscle, physiological levels of AA stimulate the phosphorylation of mTOR, which, in turn, enhances the phosphorylation of S6K1 and 4EBP1, and leads to an increased synthesis of proteins."
26394157
sparser
"MTOR could phosphorylate and activate p70s6k through interacting with it [ xref ]."
25738361
sparser
"MTOR-dependent phosphorylation of S6K1 causes S6K1 activation ( xref ), whereas mTOR-dependent phosphorylation of 4E-BP1 leads to its dissociation from the initiation factor eIF4E, thereby enabling eIF4E derepression ( xref )."
20145209
reach
"The eIF4B needs to be phosphorylated by the p70 ribosomal S6 protein kinase 1/2 (S6K1/2) to associate with the translation initiation complex (Fig. 1) [26, 27] and mTOR activation also induces phosphorylation of S6K1, thereby activating it."
26024835
reach
"On the ocher hand, the available evidence suggests that p70S6K is not phosphorylated directly by mTOR."
9638370
reach
"MTOR phosphorylated the ribosomal protein p70 S6K1 and 4EBP1 to facilitate de novo protein synthesis [7]."
34059001
sparser
"Studies show that mTOR phosphorylation of p70S6K1 increases its kinase activity; and that phosphorylation of 4E-BP1 results in the dissociation of 4E-BP1 from eIF4E and the activation of cap-dependent translational initiation xref ."
24466059
sparser
"We also examined the affect of Tel2 loss on the phosphorylation of p70S6K by mTOR."
20864032
rlimsp
"We found that overexpression of 4EBP1 promoted the apoptosis rate as well as the VP1 expression after viral infection, and overexpression of p70S6K promoted apoptosis while decreased the VP1 expression at 24 h p. i. Activated mTOR phosphorylates the downstream effectors 4EBP1 and p70S6K."
24030155
reach
"The mTOR associated small G protein Ras homolog enriched in brain (Rheb) is believed to mediate the mTOR dependent phosphorylation of both 4E-BP1 and p70S6K [148]."
16297654
sparser
"In the activated state, mTOR phosphorylates downstream ribosomal proteins S6K1 and 4EBP1."
27503318
reach
"In response to insulin and amino acids mammalian target of rapamycin (mTOR), a serine/threonine kinase, phosphorylates and modulates activity of S6K1 kinase [XREF_BIBR - XREF_BIBR]."
19781106
reach
"Temsirolimus is a dihydroxymethyl propionic acid ester of rapamycin that inhibits phosphorylation of S6K1 and 4E-BP1 mediated by mTOR."
27242542
reach
"As predicted, inhibition of mTOR kinase activity prevented p70S6K, 4E-BP1 phosphorylation and was associated with an inhibition of myogenic differentiation."
25486193
sparser
"In S6K1, mTOR does not phosphorylate the rapamycin-sensitive S/TP sites [10 • ] ."
10072357
rlimsp
"Consistent with prior studies that have demonstrated that EPO increases mTOR signaling in inflammatory cells [27], [33] and in osteoblastic phenotypes in human bone marrow stromal cells [34], we illustrate that EPO in neuronal cells requires mTOR to phosphorylate p70S6K and 4EBP1."
23029019
reach
"Additionally, the indicators of AKT and mTOR signaling, phosphorylated (active) AKT, mTOR and p70S6K were both increased by miR-181b mimics and decreased by its inhibitors."
29608948
sparser
"Western blot analysis showed significantly increased levels of phosphorylated mTOR (p-mTOR) and phosphorylated p70S6 kinase 1 (p-S6K1, a downstream effector of mTOR) in the ipsilateral L4/5 spinal cord 2h, 1 day, 3 days, and 7 days after intraplantar CFA injection and in the ipsilateral L4/5 dorsal root ganglions (DRGs) 1 and 3 days after CFA injection."
23583278
sparser
"MTOR phosphorylates S6K1 (a key regulator of cell volume), therefore mHTT-related impairment of mTOR may account for the brain atrophy in HD."
31940909
reach
"MTOR phosphorylates and activates the translational regulatory proteins eIF-4E-binding protein 1 (4E-BP1) and p70 S6 kinase (p70 S6K)."
16154531
rlimsp
"In the present study, we observed that increased insulin levels may have resulted in increased mTOR levels and phosphorylation of p70S6K; however, we have no data that directly address this idea, and previous studies [30,34] do not support the idea that typical fasting insulin concentrations (above basal levels) can stimulate the mTOR/p70S6K pathway."
21345206
sparser
"Activated mTOR induces phosphorylation of p70S6K followed by p70S6K-induced phosphorylation of S6 and the eukaryotic translation initiation factor 4B (eIF-4B), which promotes protein translation ( Wan[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22264868
reach
"Binding of growth factors activates mTOR signaling, which in turn leads to downstream phosphorylation of protein kinases, e.g., p70S6 kinase and lipid kinases in the phosphorylation of phosphoinositides."
23369283
rlimsp
"Loss of mTOR signaling prevents phosphorylation of both p70S6K and 4EBP1 to lead to apoptosis [101]."
23029019
sparser
"We found that the expressions of mTOR and S6K in the liver (Figure xref d) were not obviously changed by Dex exposure or Dex exposure with Sar or/and CDP treatment, whereas Ser 2448 phosphorylation of mTOR, and Thr 389/412 phosphorylation of p70S6K (Figure xref d) were upregulated markedly by Dex exposure, both of which were abolished significantly by Sar or CDP treatment, and abolished further by the co‐treatment, showing a synergistic effect between Sar and CDP."
27177506
reach
"MTOR also phosphorylates and activates p70S6K, which in turn phosphorylates the 40S ribosomal subunit S6 protein [XREF_BIBR]."
32998376
reach
"Akt activates mammalian target rapamycin (mTOR), which induces the phosphorylation of p70 ribosomal S6 kinase (p70S6K)."
30853887
reach
"We found that overexpression of the DGKzeta, but not of the alpha isoform, in serum deprived HEK293 cells induced mTOR dependent phosphorylation of p70S6 kinase (p70S6K)."
15632115
reach
"Now that aberrant activation of mTOR and p70S6K pathway plays an important role in tumorigenesis and phosphorylated p70S6K by mTOR has higher activity to promote translation than p70S6K [XREF_BIBR, XREF_BIBR], we speculated the combination of mTOR inhibitor and p70S6K siRNA could inhibit mTOR and p70S6K pathway at the most extent and thus inhibit tumor growth better."
27595116
reach
"The phosphorylated Akt activates mTOR, which subsequently phosphorylates p70S6K, leading to the transcription of target genes related to cell proliferation [XREF_BIBR]."
27196739
reach
"Mammalian target of rapamycin (mTOR) is a master regulator of autophagy and phosphorylates p70S6K."
30538224
reach
"In addition, mTOR phosphorylates protein phosphorylase 2A (PP2A), which prevents the dephosphorylation of S6K1 and 4E-BP1 [XREF_BIBR]."
20663687
reach
"RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1."
9465032
reach
"MTOR phosphorylates and activates S6K1 which phosphorylates the IRS proteins and both induces their degradation and reduces their interaction with the IGF1R and insulin receptors."
22576208
reach
"First, the authors showed that phospho-mTOR and its phosphorylated downstream targets, phospho-4E-BP, phospho-S6K and phospho-S6 protein, were found extensively in naïve, untreated, myelinated fibers in rat cutaneous tissue."
19490023
reach
"In other models, inhibition of mTOR causes an immediate downstream dephosphorylation of p70S6 kinase and 4E-BP1, which results in depressed translation of cell cycle proteins such as D-type cyclins."
19139116
reach
"Also, p70S6K1 phosphorylation can be mediated by an mTOR independent mechanism such as c-Jun-N-terminal kinase (JNK), which leads to a conformational change in an inhibitory domain (Thr421 and Ser424) of p70S6K1."
29024598
reach
"MTOR signaling components including mTOR phosphorylation and phosphorylation of S6K1 are elevated in aged muscle exposed to leg resistance exercise XREF_BIBR."
22701343
reach
"After processing a cell proliferative up-stream signal mediated by the PI3K and Akt pathway, mTOR phosphorylates and activates p70S6K."
22460505
reach
"Under certain conditions, including nutrient starvation or pharmacological treatment, mTOR may be inhibited, resulting in dephosphorylation of p70S6K, activation of ULK1 and initiation of autophagy."
28610619
reach
"In regards to 4EBP1, inhibition of mTOR signaling by small interfering RNA (siRNA) inhibits phosphorylation of both p70S6K and 4EBP1 that results in apoptosis [XREF_BIBR]."
23203037
reach
"XREF_BIBR When amino acids are sufficient and the AKT pathway is active, the mTOR complex 1 (mTORC1) becomes active and phosphorylates the translational regulators S6K1 and 4EBP1, stimulating their activity."
23264892
reach
"Protein synthesis is regulated in part by mTOR kinase which phosphorylates 4E-BP1 and S6K1 XREF_BIBR, XREF_BIBR."
24945486
reach
"Shuchen Gu et al [XREF_BIBR] found inhibition of mTOR blocked p70S6K and PAK1 phosphorylation and actin remodeling and demonstrated a mAR governed pathway involving FAK and PI-3K and mTOR/p70S6K/PAK1-cascade that regulates early actin reorganization in colon cancer cells."
28186966
reach
"Phosphorylation of p70S6K by mTOR is down-regulated in response to decreased amino acid availability and rapamycin treatment."
23861877
reach
"MTOR in turn phosphorylates S6K1 and 4EBP1 to directly alter protein translation."
19295495
reach
"A lack of mTOR phosphorylation of p70S6K results in reduced downstream action upon the 40S ribosomal protein S6 and eIF4B, each of which recruit ribosomes to the 5 ' end of an mRNA XREF_BIBR."
21750711
reach
"Seminal studies have shown that phosphorylated AKT induces mTOR mediated phosphorylation of p70S6K in skeletal muscle,62 resulting in muscle hypertrophy.63 The increased insulin stimulated p-p70S6K in gastrocnemius with Fst288 overexpression even though p-AKT was not significantly increased could be explained by signal amplification downstream of Akt, as p70S6K activity previously has been shown to be preserved in mice muscle with severe insulin receptor defects,64 suggesting either signal amplification or another upstream kinase regulating p70S6K in response to insulin."
31402604
sparser
"MTOR complex 1 (mTORC1) promotes phosphorylation (activation) of p70 S6 kinases (p70S6Ks) that phosphorylate the small ribosomal subunit protein S6 (Rps6), and this was proposed to promote translation of TOP mRNAs."
20509958
reach
"In contrast, loss of mTOR signaling prevents phosphorylation of both p70S6K and 4EBP1 that leads to apoptosis [69]."
24780446
sparser
"After processing a cell proliferative up-stream signal mediated by the PI3K/Akt pathway, mTOR phosphorylates and activates p70S6K."
22460505
sparser
"The mammalian target of rapamycin (mTOR) kinase phosphorylates p70 S6 kinase (p70S6K), which regulates the phosphorylation of S6 ribosomal protein (S6RP), the functional site of the 40S ribosomal subu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22217516
reach
"When complexed with Raptor, Rheb regulates mTOR mediated phosphorylation of p70 S6 kinase (S6K1) and 4E-BP1."
28099079
reach
"Likewise, knockdown of HERC1 or FRAP1 decreased P70S6K1 phosphorylation, consistent with mediation of their effects via FRAP1."
21946537
reach
"S6K1 is phosphorylated directly by mTOR during stimulation with either nutrients or growth factors."
19501448
reach
"The phosphorylation of Akt by mTOR complex 2 (mTORC2) results in full activation of this kinase [XREF_BIBR], which then activates the mTOR complex 1 (mTORC1) to phosphorylate S6K1 and dissociate 4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) from the initiation factor [XREF_BIBR]."
22137317
rlimsp
"BACKGROUND: The mammalian target of rapamycin (mTOR), which phosphorylates p70S6K and 4EBP1 and activates the protein translation process, is upregulated in cancers and its activation may be involved in cancer development."
19845851
reach
"These data are consistent with studies by Grabiner et al. which demonstrated that mTOR FAT domain mutants promoted phosphorylation of a cotransfected S6K1 cDNA construct [XREF_BIBR]."
26255626
reach
"The mTOR complex promotes phosphorylation of the translational regulators S6K1 and 4E-BP1 and is believed to have a central role in regulating cellular growth and proliferation by modulating metabolism."
23142783
sparser
"Since mTor phosphorylates and activates p70S6 kinase, inhibition of mTor by rapamycin can result in reduced p70S6 kinase activity [28] ."
15369799
sparser
"MTOR phosphorylates and activates S6K1, which then phosphorylates IRS-1 S1101 , leading to poor activation of its downstream PI-3 kinase as evidenced by weak binding of the PI-3 kinase to IRS-1 and weak AKT phosphorylation upon insulin stimulation [ xref , xref ]."
32998286
reach
"In addition, some studies have shown that oxidative stress by H 2 O 2 inhibits mTOR mediated phosphorylation of S6K1 and 4E-BP1, leading to apoptosis of neuronal cells 56."
30310136
sparser
"Activated mTOR phosphorylated p70S6K, which induces GBM formation in mice. xref S6K1 correlated with Ki67 labeling index, indicating that p-p70S6K is correlated with proliferation of tumor cells. xref After WB assay, we confirmed that cytarabine significantly decreased phosphorylation of p70S6K in a dose-dependent manner."
28721010
sparser
"Active mTOR in turn phosphorylates both the p70S6 kinase (S6K), which promotes translation elongation and ribosome biogenesis, and the eIF4E binding protein (4E-BP)."
24351799
reach
"MTOR can also phosphorylate p70 ribosomal S6 kinase (p70S6K)."
19631745
reach
"Within mTORC1, mTOR is able to phosphorylate p70 S6 kinase (S6K) and eIF4E binding protein (4E-BP1), cascades that regulate the initiation step in mRNA translation."
26314207
rlimsp
"These findings suggest that mTOR may be able phosphorylate S6K1 even in absence of eIF3."
26172298
rlimsp
"Treatment with EPO leads to mTOR activation and phosphorylation of p70S6K and 4EBP1."
23029019
reach
"XREF_BIBR Here we further show that H 2 O 2 -induced ROS inhibited mTOR mediated phosphorylation of S6K1 and 4E-BP1 in PC12 cells and primary murine neurons."
20142804
reach
"The mTOR activation phosphorylates its downstream targets S6K1 and Akt which, in turn, regulate protein translation and cell survival."
20977929
sparser
"When activated, mTOR phosphorylates the downstream target proteins, S6K1 and 4EBP1, leading to the dissociation of eIF4E•4EBP1 and the formation of the active eIF4G•eIF4E complex which enhances translation initiation."
23736770
reach
"The kinase responsible for these phosphorylations is mTOR (mammalian target of rapamycin) (Schmelzle and Hall, 2000; Raught et al., 2001), which can also phosphorylate the kinase p70S6K that stimulate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18809477
reach
"In MAC-T cells, ATRA increased the mRNA levels of alpha S1 -casein and beta-casein, janus kinase 2 (JAK2) and E74 like factor 5 of the signal transducer and activator of transcription 5 beta (STAT5-beta) pathway, ribosomal protein S6 kinase beta-1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 of the mammalian target of rapamycin (mTOR) pathway, inhibited the mRNA expression of phosphoinositide 3-kinase and eukaryotic initiation factor 4E of the mTOR pathway, and promoted the phosphorylation of STAT5-beta and S6K1 proteins."
31480153
reach
"By binding to FK binding protein 12, it inhibits the functioning of a specific cell regulation protein of cell growth, the mammalian target of rapamycin (mTOR), which causes reduced phosphorylation of p70 S6 kinase further down the signal transduction pathway [XREF_BIBR - XREF_BIBR]."
22721369
sparser
"MTOR phosphorylated the ribosomal protein p70 S6K1 and 4EBP1 to facilitate de novo protein synthesis [ xref ]."
34059001
sparser
"MTOR phosphorylates both p70S6K and eukaryotic initiation factor 4E-binding protein (4E-BP1) via independent pathways."
26894018
reach
"In addition, mTOR, another substrate subjected to phosphorylation by AKT, enhances phosphorylation of S6K1 and 4E-BP1 [XREF_BIBR] and plays crucial roles in the regulation of ribosomal protein synthesis, for example, production of cyclin D1 and VEGF-A at both transcriptional and translational levels [XREF_BIBR, XREF_BIBR]."
26265437
reach
"MTOR signalling mediates the phosphorylation of eIF4EBP1 and the ribosomal protein p70S6K, thereby affecting the translation of mRNA [XREF_BIBR]."
23552196
reach
"Activated mTOR increases protein translation through phosphorylation of 4EBP1 and p70S6K."
21276607
sparser
"Treatment of cells with [sorafenib and HDAC inhibitor] + TRAIL reduced AKT, p70 S6K and mTOR phosphorylation at regulatory sites."
23674352
reach
"MTOR complex 1 (mTORC1) promotes phosphorylation (activation) of p70 S6 kinases (p70S6Ks) that phosphorylate the small ribosomal subunit protein S6 (Rps6), and this was proposed to promote translation of TOP mRNAs."
20509958
reach
"We found that overexpression of 4EBP1 promoted the apoptosis rate as well as the VP1 expression after viral infection, and overexpression of p70S6K promoted apoptosis while decreased the VP1 expression at 24h p. i. Activated mTOR phosphorylates the downstream effectors 4EBP1 and p70S6K."
24030155
reach
"WISP1 promotes mTOR activation and phosphorylation of p70S6K and 4EBP1."
22873724
reach
"Furthermore, inhibition of MTORC1 activation by rapamycin or siRNA mediated knockdown of Mtor abolished the insulin induced phosphorylation of RPS6KB1 and its target RPS6, and EIF4EBP1 phosphorylation."
23261705
reach
"Inhibition of upstream mTOR via MK-8669 effectively blocked S6K1 phosphorylation and in conjunction with previous findings, increased AKT phosphorylation likely via negative feedback loops secondary to mTOR inhibition."
27765027
sparser
"Protein synthesis is primarily regulated by the mammalian target of rapamycin (mTOR) that phosphorylates S6K-1 and 4E-BP1, thereby stimulating translation."
33222219
sparser
"Under hormone treatment, inhibition of PPARγ activity decreased the ratio of PGE 2 /PGF2α and cellular activity, while activating expression of the mTOR downstream marker-the phosphorylation of p70S6K (p-p70S6K)."
36423098
reach
"Similarly, the p70S6K kinase, which is phosphorylated and activated by mTOR in mitogenic pathways downstream of PI3K and AKT, was not differently phosphorylated in tamoxifen treated EO771 cells compared to the control condition."
32699527
reach
"Studies have reported that many cancer cells abnormally activate AKT and PI3K, leading to the activation of mTOR, which activates protein synthesis by phosphorylating downstream p70S6K and 4EBP1 to regulate cancer cell proliferation [152]."
31835352
sparser
"MTOR also phosphorylates and activates p70S6K, which in turn phosphorylates the 40S ribosomal subunit S6 protein [ xref ]."
32998376
sparser
"MTOR phosphorylates and activates both p70S6K and 4EBP1 that serve as markers of mTOR activity."
24780446
reach
"The mammalian target of rapamycin (mTOR) kinase phosphorylates p70 S6 kinase (p70S6K), which regulates the phosphorylation of S6 ribosomal protein (S6RP), the functional site of the 40S ribosomal subu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22217516
rlimsp
"Data published recently have led to the view that FRAP acts as a traditional mitogen-activated kinase, directly phosphorylating 4E-BP1 and p70(s6k) in response to mitogenic stimuli."
10200280
reach
"MTOR activation further phosphorylates its two downstream targets, p70S6K and 4E-BP1."
| PMC
reach
"By contrast, our data were consistent with findings in neuron cells, where rotenone induced reactive oxygen species (ROS) led to cell apoptosis, and simultaneously rotenone dramatically inhibited mTOR[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29621478
rlimsp
"EPO promotes cellular protection through mTOR activation and phosphorylation of p70S6K and 4EBP1."
23029019
reach
"It is possible that rapamycin had been effective in mTOR inhibition, yet S6K1 and 4E-BP1 were activated via mTOR independent phosphorylation of S6K1 and 4E-BP1 (a mechanism suggested by other studies 24)."
24675464
sparser
"The turn motif phosphorylation helps to dock the HM segment to a hydrophobic pocket in the N-lobe formed by the αC helix, and phosphorylation of activation loop results in ordering of the activation segment clearing the way for the mTOR to phosphorylate the HM site of S6K1."
21561857
reach
"In addition, mTOR, another substrate subjected to phosphorylation by Akt, enhances phosphorylation of S6K1 and 4E-BP1 and plays a crucial role in the regulation of ribosomal protein synthesis, for example, production of cyclin D1 and VEGF-A at both the transcriptional and translational levels XREF_BIBR."
27712596
sparser
"In particular, mTOR (Ser2448) phosphorylation as well as phosphorylation of eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) and p70S6K were unchanged after 14 days of unloading in gastrocnemius muscle with GSK-3β deletion [ xref ]."
34064895
reach
"Activated PI3K and Akt may positively regulate mTOR, which further causes phosphorylation of ribosomal p70 S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E (eIF4E) binding protein 1 (4E-BP1), th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27235588
rlimsp
"Results from published studies showed that mTOR-dependent phosphorylation of p-S6K is not linearly correlated with 4EBP1 phosphorylation3738, which could partly explain the lack of prognostic value of p-S6K observed in this study."
27026382
rlimsp
"During loss of mTOR activity or the gene silencing of mTOR, phosphorylation of mTOR, p70S6K, and 4EBP1 is minimal or absent during administration of EPO with OGD, demonstrating that EPO relies upon mTOR to phosphorylate p70S6K and 4EBP1 (Figure 2A)."
23029019
reach
"S6K1 is phosphorylated and activated by mTOR, and a reduction in the phosphorylation of S6K1 is associated with the loss of the cellular capacity to synthesize proteins."
26508818
reach
"Extracellular TG2 promoted the phosphorylation of Akt, mechanistic target of rapamycin (mTOR), and ribosomal p70 S6 kinase, and inhibitors of mTOR, PI3K, and Src (rapamycin, LY294002, and Src I1, respectively) inhibited TG2 increased phosphorylation of mTOR and p70S6K."
31666191
reach
"This allows the formation of an active TSC1 and TSC2 dimer that inhibits the phosphorylation of Ribosomal Protein S6 Kinase B1 (RPS6KB1) by the mTOR protein complex, which in turn inhibits the phosphorylation of Ribosomal Protein S6 (RPS6), part of the 40S ribosomal subunit required for initiation [XREF_BIBR]."
33477877
reach
"Thus, our data suggest mTOR-independent phosphorylation of p70S6 kinase in FPLD2 cells, a mechanism previously reported , which deserves further investigation."
31375660
reach
"A regulatory associated protein of MTOR (RPTOR) facilitates the phosphorylation of RPS6KB1 and 4EBP1 by MTOR and has been shown to bind both proteins."
23188744
sparser
"SESN2 inhibits mTOR-dependent phosphorylation of p70S6K and 4E-BP1,( xref )and knockdown of SESN2 resulted in the activation of mTOR signaling indicating that the important role of SESN2 on mTOR inhibition.( xref )Our results from western blotting demonstrated that mTOR was down-regulated by fisetin treatment in HNCCs consistent with other previous studies showing tumor inhibiting potentials of fisetin through mTOR pathway.( xref , xref , xref )These results suggest that fisetin-induced apoptotic potentials is through SESN2/mTOR signaling axis."
30936621
reach
"The p70S6 kinase was phosphorylated by activation of the mammalian target of rapamycin (mTOR) signal pathway, which has been shown to promote neuronal growth and LTP [XREF_BIBR, XREF_BIBR]."
33505460
rlimsp
"EPO employs mTOR to phosphorylate p70S6K and 4EBP1."
23029019
sparser
"PDK1 was previously shown to mediate activation of the serine-threonine kinase Akt by P13K. On the ocher hand, the available evidence suggests that p70S6K is not phosphorylated directly by mTOR."
9638370
reach
"In the canonical IGF-1/Akt/mTORC1 pathway, Akt activation of mTOR and subsequent phosphorylation of p70S6K and 4E-BP1 will induce protein synthesis."
23116773
sparser
"Western blotting showed decreased expression of phosphorylated mTOR and phosphorylated p70S6K in the rapamycin-treated group, and increased the expression of both SIRT1 and SIRT6 compared to the control group (P<0.05)."
23566837
reach
"Although BDNF stimulates both extracellular Ca 2+ influx and intracellular Ca 2+ release, it is not clear how these dynamic changes in calcium regulate the mTOR dependent phosphorylation of p70S6K."
20029971
reach
"Activated PI3K and Akt may positively regulate mTOR, which further causes phosphorylation of ribosomal p70 S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E (eIF4E) binding protein 1 (4E-BP1), th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27235588
sparser
"HPC could inhibit phosphorylated mTOR (Ser2448) and phosphorylated p70S6K (Thr389) and increase the ratio of autophagy markers LC3-II/LC3-I in the CA1 and CA3 regions ( P < 0.05; Figures xref – xref )."
36120601
reach
"P70S6K is phosphorylated directly by mTOR and can be used as an indicator of mTOR activity."
32467173
reach
"Upon activation by proliferative stimuli mediated by the PI3K and Akt signal transduction pathway, mTOR phosphorylates and activates p70S6K, which in turn phosphorylates the 40S ribosomal protein S6."
25729255
reach
"Interestingly, the study has also found that autophagy activators decrease the cytotoxicity of fenofibrate, while autophagy inhibitors increase the fenofibrate induced glioblastoma cytotoxicity including phosphorylation of AMPK and suppression of mTOR- dependent phosphorylation of p70S6K."
33833983
reach
"MTOR is eventually activated and induces the phosphorylation of ribosomal protein S6 and its complex p70 S6 kinase (S6K) to promote ribosome biosynthesis, thereby promoting hyperactive HIF-1alpha translation [XREF_BIBR]."
| PMC
reach
"In the activated state, mTOR phosphorylates downstream ribosomal proteins S6K1 and 4EBP1."
27503318
reach
"MTOR phosphorylates p70 S6 kinase (p70S6K) and the 4E-BP1 translational repressor, leading to translation of proteins required for cell proliferation."
19690197
reach
"Mechanistic studies showed that inhibition of the mTOR pathway by rapamycin alone sufficiently suppressed the phosphorylation of the downstream molecules p70S6K and 4E-BP-1, but only caused a moderate cytostatic effect."
16193082
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"The phosphorylation of T412 is highly rapamycin sensitive and correlates very closely with T252 phosphorylation and full activation of S6K.Early reports linked mTOR to the control of S6K activation in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20932932
reach
"This activation leads to the phosphorylation and activation of mammalian target of rapamycin (mTOR), which causes phosphorylation and activation of p70S6 kinase and 4E-BP1, and inhibition of glycogen synthase kinase 3-beta, which is a repressor of eIF-2B."
19656243
reach
"A downstream effector of the Akt pathway is the mTOR kinase, which in turn promotes the phosphorylation of p70S6K, leading to an increase in protein synthesis."
21798081
sparser
"MTOR phosphorylates and activates p70S6K at threonine 389 which serves as a marker of mTOR activity ( xref )."
22873724
reach
"MTOR phosphorylates p70S6 kinase (p70S6K) at several residues, resulting in increased kinase activity."
24662921
sparser
"It is unlikely, that this effect is related to the mTOR-dependent phosphorylation of S6K1 and rpS6, because similar dephosphorylation of both proteins occurs by incubation of cells with the mTOR kinase inhibitor pp242."
25294810
reach
"Therefore, the interaction between S6K1 and eIF3-PIC appears to be regulated by mTOR dependent phosphorylation of S6K1 at T389.We confirmed the contribution of S6K1 T389 phosphorylation to binding eIF[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16286006
sparser
"Direct phosphorylation of 4E-BP1 and S6K1 by mTOR, or indirect regulation of phosphorylation by an mTOR-regulated kinase or phosphatase, has been suggested ."
12747827
sparser
"Now that aberrant activation of mTOR/p70S6K pathway plays an important role in tumorigenesis and phosphorylated p70S6K by mTOR has higher activity to promote translation than p70S6K [ xref , xref ], we speculated the combination of mTOR inhibitor and p70S6K siRNA could inhibit mTOR/p70S6K pathway at the most extent and thus inhibit tumor growth better."
27595116
reach
"Furthermore, the infusion of IL-6 in skeletal muscle can reduce the phosphorylation of S6K1, which is activated by Akt and mTOR and associated with the inhibition of anabolic process [XREF_BIBR]."
27382188
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"It was our previous finding that mTOR inhibition by selective inhibitors pp242 and Torin2, that suppress phosphorylation of 4E-BP and p70S6 kinase, caused in the cell specific inhibition of both YB-1 synthesis and translation of reporter mRNAs carrying the 5 ' UTR of YB-1 mRNA."
26931209
sparser
"We also found that LPAR5 specific antagonists TC LPA5 4, PI3K inhibitor Wortmannin or mTOR inhibitor Rapamycin pretreatment abrogated phosphorylation of Akt and p70S6K1 stimulated by LPA in CGTH-W3 and TPC-1 cells."
33540491
reach
"These mTOR kinase inhibitors such as Torin1, PP242 and PP30 block p70S6K, 4E-BP1 and AKT phosphorylation and thus inhibit both mTORC1 and mTORC2 [XREF_BIBR, XREF_BIBR]."
23991179
reach
"PRL activated mTOR may phosphorylate p70S6K and 4E-BP1 by restraining PP2A."
16899564
reach
"MTOR regulates protein synthesis by phosphorylating the key substrates of the translational machinery, namely, the eukaryotic initiation factor 4E-binding protein and p70S6 kinase."
34489627
rlimsp
"Moreover, inhibition of mTOR activity by rapamycin significantly inhibited the promoting effect of TRAF4 on phosphorylation of p70s6k, Although there was still a seemingly weak upregulation of p-S6, it was not significant."
25738361
reach
"Our data indicate that Rac1 also functions upstream of mTOR, since mTOR phosphorylation of both 4eBP1 and p70 S6 kinase was inhibited by Rac1 deletion."
21474067
sparser
"The mTOR complex promotes phosphorylation of the translational regulators S6K1 and 4E-BP1 and is believed to have a central role in regulating cellular growth and proliferation by modulating metabolism."
23142783
reach
"MTOR integrates stimulating signals and phosphorylates eukaryotic initiation factor 4E binding protein 1 (4E-BP1) and p70 ribosomal protein S6 kinase (p70S6K) XREF_BIBR, XREF_BIBR."
25048611
rlimsp
"RAFT1 phosphorylates p70(S6k) much more effectively than 4E-BP1, and the phosphorylation sites on the two proteins show little homology."
9465032
sparser
"Of note, PDK1A280V has been reported to activate p70S6K despite the fact that wtPDK1/p70S6K interactions require prior p70S6K phosphorylation by mTor ( xref ; xref )."
18971483
sparser
"cAMP elevation has also been shown to inhibit IL-2-stimulated activation of PI3K and downstream, mammalian target of rapamycin-dependent phosphorylation of p70 s6 kinase in T cells ( xref )."
20515836
reach
"Amino acid signals could regulate the translation of proteins required for trophoblast differentiation via mTOR dependent phosphorylation of S6K1 XREF_BIBR."
22848442
reach
"In the cells with empty vector or mTOR wild type, phosphorylation of S6K1 was not seen (XREF_FIG), whereas mTOR active mutants, E2419K and L1460P, increased phosphorylation of S6K1."
20190810
rlimsp
"This may be due to that Rapamycin can not completely inhibit the phosphorylation and activation of p70s6k by mTOR, which also can be explained by the presence of Rapamycin-insensitive mTOR (Figure 4D)."
25738361
sparser
"On the one hand, mTOR phosphorylates and activates p70S6K (p70 ribosomal protein S6 kinase), furthermore, to involve in phosphorylation/inactivation of Bcl-2 [ xref , xref ]."
28638431
reach
"Inhibition of mTOR signalling leads to dephosphorylation of 4E‐BP1 and p70S6K1 to induce autophagy."
30247801
rlimsp
"These results indicate that mTOR is likely to function as a direct activator of p70 in vivo, although the relative contribution of mTOR-catalyzed p70 phosphorylation in each of the many circumstances that engender p70 activation remains to be defined."
10567431
reach
"In response to mitogen stimulation, mTOR phosphorylates and activates S6K1, which in turn phosphorylates the 40S ribosomal protein S6, leading to the enhancement of translation of mRNAs with a 5 '-terminal oligopyrimidine, including mRNAs that encode for ribosomal proteins and elongation factor-1."
19437488
sparser
"This may be due to that Rapamycin can not completely inhibit the phosphorylation and activation of p70s6k by mTOR, which also can be explained by the presence of Rapamycin-insensitive mTOR (Figure xref )."
25738361
reach
"Consequently, phosphorylation of the S6K1 fragment by mTOR would appear to be raptor independent, and might depend instead on passive diffusion of the substrate into the active site of the mTOR kinase[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12372295
reach
"Because mTOR phosphorylates S6K1, an event that is critical for maximal activation of the kinase, this finding suggests that portal AA delivery increases mTORC1 signalling in liver to a greater extent than peripheral delivery."
18842785
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"The first component of the signaling pathway identified was p70 ribosomal S6 kinase1 (S6K1), which is phosphorylated and activated by mTOR [XREF_BIBR, XREF_BIBR]."
21354266
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"60 S6K1 is phosphorylated and activated by mTOR to promote translation and growth by phosphorylating cellular substrates, including ribosomal S6, leading to increased ribosomal biogenesis."
23542173
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"S6K1, a serine/threonine kinase, is phosphorylated and activated by mTOR to promote cell growth, proliferation and differentiation ."
30442943
sparser
"Similarly, phosphorylation of p70 S6 kinase by mTOR has been reported ()."
10580846
reach
"3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2 (3H)-one (3BDO), a small molecular compound, has been demonstrated to be a novel mTOR activator that can significantly increase the phosphorylation of RPS6KB1 and EIF4EBP1 in HUVECs [XREF_BIBR]."
27071307
reach
"Since activated Akt and PDK1 may positively regulate mTOR and S6K1, respectively, XREF_BIBR, we next investigated whether H 2 O 2 induction of ROS inhibits mTOR mediated phosphorylation of mTOR and S6K1 by suppressing the activity of Akt and PDK1, respectively."
20142804
reach
"Stability of long-term fear memory is compromised if mTOR activation and phosphorylation of p70S6 kinase is altered."
23091460
sparser
"Moreover, sequential phosphorylation of p70alpha by mTOR and 3-phosphoinositide-dependent protein kinase 1 in vitro resulted in a synergistic stimulation of p70alpha activity to levels similar to that attained by serum stimulation in vivo."
10567431
sparser
"Phosphorylation of S6K1 on Thr390 (human Thr389) is a primary activator of its kinase activity and appears to regulate translation of ribosome-related transcripts and ribosome biogenesis, although the mechanism underlying differential regulation of phosphorylation of Eif4ebp1 and S6K1 by Frap1 is currently unknown xref ."
19370158
sparser
"MTOR complex phosphorylates S6K1 and regulates ribosomal protein synthesis."
19501448
sparser
"Activated mTOR phosphorylates p70S6K1 that promotes gene expression, while activated mTOR inhibits 4E-BP1 through phosphorylation that up-regulates gene expression."
28676430
sparser
"S6K1 is phosphorylated directly by mTOR during stimulation with either nutrients or growth factors."
19501448
reach
"MTOR activation also results in the phosphorylation of p70S6 kinase, which activates TOP dependent translation."
21484200
reach
"Moreover, mTOR signaling can activate chromatin state of the RP genes by recruiting histone acetyltransferase ESA1, and phosphorylate p70 RP S6 kinase 1."
32655613
reach
"MGluR activation of PI3K, mTOR and ERK also stimulates phosphorylation of p70 ribosomal S6 kinase (RSK) and ribosomal S6 in hippocampal slices which functions to increase translation of a subset of mRNAs (those with a 5 ' terminal oligopyrimidine tract; 5 ' TOP) that encode ribosomes, translation factors, thus increasing the overall translational capacity of the neuron."
20188650
sparser
"Moreover, Raptor-free mTOR is capable of phosphorylating S6K1 but not 4E-BP1, providing a basis for the differential regulation of these two substrates by mTORC1[ xref ]."
29357370
sparser
"During viral translation NS5A activates m ammalian t arget o f R apamycin (mTOR) pathway by disrupting the interaction between FK506-binding protein 38 (FKB38) and mTOR. xref , xref Activated mTOR initiates phosphorylation of ribosomal protein S6 kinase beta-1 (S6K1) protein, which eventually phosphorylates ribosomal protein 6 (rps6). xref Expression of NS5A increases the level of phosphorylated pS6K1."
28442256
sparser
"The well-known downstream signaling pathways of PI3K/Akt that are involved in protein synthesis are the mammalian target of rapamycin (mTOR)-mediated phosphorylation and activation of p70S6 kinase (p70 S6K ) and 4E-BP1 ( xref )."
28587873
reach
"Inhibition of either mTOR by rapamycin or PI3K by LY249002 in naive CD4 cells blocks phosphorylation of S6K1 and S6 and prevents phosphorylation."
22079331
reach
"We found that overexpression of the DGKzeta, but not of the alpha isoform, in serum deprived HEK293 cells induced mTOR dependent phosphorylation of p70S6 kinase (p70S6K)."
15632115
sparser
"It was reported that mTOR could phosphorylate S6K1 and inhibits the initiation factor 4EBP to initiate the translation of distinct mRNAs in mammals ( xref )."
36177013
reach
"5 Although rapamycin rapidly inhibits phosphorylation of p70S6K by mTOR, 4E-BP1 phosphorylation slowly decreases within 2h."
25361081
reach
"Global protein translation is generally suppressed under hypoxic conditions, and in our study, p70 S6 kinase, which regulates global translation and can be phosphorylated by mammalian target of rapamycin (mTOR), was inactivated in hypoxia (data not shown)."
19843849
reach
"Previous studies reported that late LTP- or mGluR-LTD-inducing stimulation induced mTOR mediated phosphorylation of downstream targets, 4E-BP and p70S6K, and subsequent activation of translation machi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23348097
reach
"Autophagy induction in cisplatin treated cells was preceded by activation of adenosine monophosphate activated protein kinase (AMPK) and concomitant down-regulation of mammalian target of rapamycin (mTOR)-mediated phosphorylation of p70S6 kinase."
20196784
reach
"This result further substantiated that everolimus inhibited the activation of mTOR, so that mTOR was unable to phosphorylate p70 S6 K. Everolimus Decreases Expression of Genes That Regulate Glycolytic Proteins and Enzymes."
27140422
reach
"Specifically as part of mTORC1, mTOR directly phosphorylates the ribosomal protein S6 kinases (S6K1 and S6K2) and the eukaryotic initiation factor 4E (eIF4E)-binding proteins (4E-BP1 and 4E-BP2), both of which control specific steps in the initiation of cap dependent translation."
20670887
reach
"The phosphorylation of S6K1 by mTOR is confined to a single residue (T389), and this phosphorylation was eliminated by pemetrexed (XREF_FIG), whereas four residues on 4EBP1 are phosphorylated by mTOR; the broader migration pattern seen in pemetrexed treated cells with the pan-4EBP1 antibody (XREF_FIG) suggests that several of these phosphorylation sites are affected by drug."
19549896
reach
"In mammalian cells, protein translation is mainly regulated by the mammalian target of rapamycin (mTOR), which phosphorylates among others the two key protein translation regulators p70S6K and 4-EBP1 [XREF_BIBR]."
24009772
reach
"When complexed with Raptor, Rheb regulates mTOR mediated phosphorylation of p70 S6 kinase (S6K1) and 4E-BP1."
28099079
reach
"For example, mTOR, a downstream target of Akt, phosphorylates p70S6 kinase and 4E-BP1 in response to growth factor stimulation [29-32]."
21315178
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"RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1."
20019839
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"Burnett PE, Barrow RK, Cohen NA, Snyder SH, Sabatini DM : RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1."
9561852
reach
"MTOR activation results in phosphorylation of effector molecule p70S6K and S6 ribosomal protein, which subsequently leads to mTOR dependent gene transcription that regulates cell proliferation, protei[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21094138
sparser
"Once this has occurred, mTOR then phosphorylates P70s6k and 4E-BP1."
28566814
rlimsp
"P70S6K has been shown to be phosphorylated by mTOR [10], a downstream signaling molecule in the phosphoinositide-3 kinase (PI3K) pathway [11], [12]."
22900063
reach
"When BCAA levels are sufficient, mTOR is activated and causes an increase in p70S6K phosphorylation, subsequently activating the S6 ribosomal protein, thereby inducing protein synthesis.9, 10 In contrast, at low BCAA concentrations, mTOR recognizes the limited substrate availability for protein synthesis and recycling and inhibits p70S6K phosphorylation, hindering normal protein recycling, and thus allowing ' wear and tear ' protein damage to accumulate."
29774990
rlimsp
"Since amino acids activate mTOR partly independent of insulin, and since mTOR phosphorylates S6k, the authors [21] suggested that increased amino acid availability could increase insulin-unstimulated protein synthesis through mTOR-S6k independent of Akt."
21483870
sparser
"High mTOR activity results phosphorylation of S6K1 protein, and subsequent phosphorylation of rps6. xref , xref , xref Presumably the phosphorylated rps6 is necessary for enhanced translation from HCV IRES."
28442256
sparser
"TNFα induces mTOR activation, and then mTOR phosphorylates its target S6K1."
26343727
rlimsp
"Multiple phosphorylation sites have also been found on the serine and threonine residues of p70S6K, however, phosphorylation of threonine 389 residue by mTOR is critical for p70S6K activation and serves as a marker for mTOR activity [87]."
21307646
reach
"It was previously demonstrated by our group (Pereira et al., 2012) that rapamycin inhibits formation of mTOR complex 1 (mTORC1) and 2 and insulin stimulated phosphorylation of p70S6K and protein kinas[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23160140
reach
"Activated PI3K and Akt may positively regulate mTOR, which further causes phosphorylation of ribosomal p70 S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E (eIF4E) binding protein 1 (4E-BP1), the two best characterized downstream effector molecules of mTOR [XREF_BIBR, XREF_BIBR]."
27235588
sparser
"As the drug combination also inhibited both p70 S6K and mTOR phosphorylation we introduced activated forms of p70 S6K and mTOR into cells and determined the response to [lapatinib + sorafenib] treatment."
24911215
reach
"The influence of mTOR on translation and ultimately protein synthesis is commonly mediated by mTORC1 induced phosphorylation of p70S6K and 4EBP1."
23798306
reach
"Growth factor activation of PI 3-kinase/Akt results in the phosphorylation and inhibition of TSC1-TSC2, leading to derepression of mTOR, which can then phosphorylate 4E-BP1 and p70 S6 kinase [10]."
12935885
reach
"MTOR associates with Raptor (mTORC1 complex) to phosphorylate p70 S6 kinase, which in turn phosphorylates 4E-BP1, leading to increased cell proliferation."
22532602
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"The activation of mTOR, in turn, induces the phosphorylation of p70S6K (activates the ribosomal subunit required for muscle protein translation) XREF_BIBR and the translational repressor 4E-BP1, which is inactivated following phosphate addition XREF_BIBR."
23239980
reach
"Leucine enhances mTOR mediated phosphorylation of S6K1 and 4E-BP, thereby promoting protein synthesis."
20298436
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"The aforementioned findings showed that upregulated DAPK2 prevented the placental cells from oxidative damage in HDCP, but the protection was invalid after the mTOR signaling pathway inactivation.The [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30243997
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"We found that AKT and mTOR signaling was significantly inhibited in the MMQ and GH3 cells by CAB in a time dependent manner as indicated by decreased phosphorylation of AKT and mTOR as well as decreased phosphorylation of p70S6K and 4EBP1, two key downstream effectors of the mTOR pathway."
26513171
reach
"MTOR complex 1 (mTORC1) phosphorylates S6K1, while mTORC2 phosphorylates Akt."
18411301
sparser
"In a previous study, we showed that mTOR could phosphorylate p70S6K at Thr 421/Ser 424, a specific site of ERK and inversely, ERK could phosphorylate p70S6K at Thr 389 controlled by mTOR signalling ( [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18809477
sparser
"In addition, mTOR, another substrate subjected to phosphorylation by Akt, enhances phosphorylation of S6K1 and 4E-BP1 and plays a crucial role in the regulation of ribosomal protein synthesis, for example, production of cyclin D1 and VEGF-A at both the transcriptional and translational levels xref ."
27712596
reach
"The mTOR complex1 phosphorylates S6K1, which stimulates translation through activation of S6 Ribosomal Protein."
29526763
sparser
"A lack of mTOR phosphorylation of p70S6K results in reduced downstream action upon the 40S ribosomal protein S6 and eIF4B, each of which recruit ribosomes to the 5′ end of an mRNA xref ."
21750711
sparser
"Moreover, overexpression of LPAAT-θ has been found to induce mTOR-dependent p70S6K1 and 4E-BP1 phosphorylation and is expressed in skeletal muscle tissue [ xref ]."
28184235
sparser
"This condition—active mTOR with reduced translational capacity—reminds the condition of cycloheximide treatment, in which mTOR phosphorylates pS6K [ xref ] although growth is impaired, thus, producing the effect of a counter circuit in the cell."
33207837
reach
"Inhibition of PI3K or mTOR and p70S6K by wortmannin and rapamycin, respectively, increased apoptosis and inhibited phosphorylation of Akt and p70S6K induced by single-dose oxidative stress."
20230819
sparser
"S104/106 are proline-directed sites, while S118 resides within a sequence that resembles a hydrophobic motif, which are phosphorylated by mTOR in its substrates 4E-BP1 and S6K1, respectively (Reviewed in xref , xref )."
26522726
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"Stimulation of the mTOR pathway by adding leucine and insulin increased the phosphorylation of p70S6K without inactivation of AMPK."
25971373
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"Mammalian target of rapamycin complex 1 both inhibits autophagy and phosphorylates p70S6K (Zhou and Huang, 2010)."
27094592
reach
"M-TOR binds with Raptor and phosphorylates p70S6 kinase, a protein involved in numerous cell signalling pathways."
22863900
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"P70 S6 kinase, which is phosphorylated by mammalian target of rapamycin complex 1 (mTORC1) downstream of AKT, showed increased phosphorylation levels at ZT 16-22."
30210362
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"Rapamycin, an inhibitor of mTOR, abolished diabetes induced changes in phosphorylation of eEF2, eEF2 kinase, and p70S6 kinase and ameliorated renal hypertrophy and laminin-beta1 protein content, without affecting hyperglycemia."
17991718
reach
"Hamartin, encoded by TSC1, and tuberin, encoded by TSC2, form a complex, which functions as a tumour suppressor by inhibiting mammalian target of rapamycin complex 1, which phosphorylates p70S6K and 4E-BP1."
30794603
sparser
"Although BDNF stimulates both extracellular Ca 2+ influx and intracellular Ca 2+ release, it is not clear how these dynamic changes in calcium regulate the mTOR-dependent phosphorylation of p70S6K. Our data show that the BDNF-mediated Ca 2+ elevation may not be required."
20029971
reach
"Mammalian target of rapamycin (mTOR) kinase phosphorylates p70 S6 kinase (p70S6K), which regulates the phosphorylation of S6 ribosomal protein (S6RP), the functional site of the 40S ribosomal subunit."
27632912
reach
"4E-BP1 and S6K1 are phosphorylated by mammalian target of rapamycin complex 1 (mTORC1) which regulates the binding of eIF4E to the mRNA 5 ' cap through phosphorylation of 4E-BP1 and stimulates protein synthesis through the phosphorylation of S6K1 XREF_BIBR XREF_BIBR XREF_BIBR XREF_BIBR."
28051178
sparser
"In addition, mTOR, another substrate subjected to phosphorylation by AKT, enhances phosphorylation of S6K1 and 4E-BP1 [ xref ] and plays crucial roles in the regulation of ribosomal protein synthesis, for example, production of cyclin D1 and VEGF-A at both transcriptional and translational levels [ xref , xref ]."
26265437
rlimsp
"Indeed, MAP4K3 is required for phosphorylation of known mTOR targets such as S6K1 (S6 kinase 1), and overexpression of MAP4K3 promotes the rapamycin-sensitive phosphorylation of these same targets."
17346240
reach
"MTOR phosphorylates the kinase p70S6 kinase1 (S6K1) (Holz et al., 2005)."
31681793
sparser
"In addition, mTOR positively regulated the phosphorylation of ribosomal p70S6 kinase (p70S6k), which subsequently lead to mTOR-dependent gene transcription that regulated cell proliferation."
35056164
sparser
"Additionally, mTOR can induce the phosphorylation of p70S6 kinase (S6K), which also promotes HIF-1α protein synthesis [13]."
34360716
sparser
"The second involves the activation of mammalian target of rapamycin (mTOR) which phosphorylates the eIF4E repressor protein, 4E binding protein 1 (4EBP1) and 70 kDa ribosomal protein S6 kinase 1 (S6K1), both of which regulate the binding of mRNA to the 43S ribosomal complex."
23736770
sparser
"Moreover, mTOR induces S6K1 (Thr 389 ) phosphorylation that promotes S6K1 nucleus translocation [43] ."
28315428
sparser
"Activated mTOR phosphorylates the downstream effectors 4EBP1 and p70S6K. Being responsible for the cap-dependent translation of mRNA and the protein synthesis, they are critical components in a pathway regularly co-opted by viruses to ensure translation of their own mRNA. xref , xref Our previous work has shown that CVB3 infection can activate mTOR/4EBP1 pathway to promote the synthesis and release of the viral protein. xref In this study, CVB3 infection directly stimulated PI3K/Akt pathway, keeping the mTOR/4EBP1 pathway activating."
24030155
reach
"MTOR silencing using an shRNA revealed that mTOR by itself only induced AKT and S6K1 dephosphorylation XREF_FIG."
29357370
reach
"The raptor and mTOR complex phosphorylates the rapamycin sensitive forms of S6K1, while the distinct rictor and mTOR complex phosphorylates the rapamycin resistant mutants of S6K1."
| PMC
reach
"The mTOR pathway, a significant controlling factor for MPS, stimulates the kinase activity of the complex and leads to the phosphorylation of 4E-BP1 and p70S6K, which are two enzymes that also modulate protein synthesis at the level of mRNA translation initiation [XREF_BIBR, XREF_BIBR]."
32460884
sparser
"Activated mTOR phosphorylates p70S6-kinase 1 (p70S6k1) and 4E binding protein 1 (4E-BP1), leading to translation of proteins involved in cell proliferation and growth ( xref ; xref )."
19639601
reach
"The mTOR signaling pathway activator MHY1485 would increase the phosphorylation of downstream ribosomal S6 kinase (S6K1) and ribosomal protein S6 (rpS6) in the ovarian tissue of mice."
33613618
reach
"The mTOR kinase lies downstream of Akt and positively regulates phosphorylation of p70 S6 kinase (S6K) and eukaryotic initiation factor 4E (eIF4E) binding protein 1 (4E-BP1)."
19661333
reach
"MTOR itself phosphorylates S6 kinase 1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 (eIF4E-BP1), leading to enhanced protein translation."
24204377
sparser
"The phosphorylation of T412 is highly rapamycin sensitive and correlates very closely with T252 phosphorylation and full activation of S6K. Early reports linked mTOR to the control of S6K activation [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20932932
sparser
"Autophagy induction in cisplatin-treated cells was preceded by activation of adenosine monophosphate-activated protein kinase (AMPK) and concomitant down-regulation of mammalian target of rapamycin (mTOR)-mediated phosphorylation of p70S6 kinase."
20196784
reach
"MTOR phosphorylates the p70 ribosomal S6 kinase (p70S6K) leading to phosphorylation of the ribosomal protein S6, which stimulates mRNA translation rates."
25257176
reach
"During terminal muscle differentiation a conserved TOS motif in eIF3f connects mTOR and raptor complex, which phosphorylates S6K1 and regulates downstream effectors of mTOR and Cap dependent translation initiation."
20126553
reach
"The phosphorylation of p70S6K and 4EBP1 by mTOR and the phosphorylation downstream of ribosomal protein S6 and EIF-4B stimulate translational initiation and contribute to cell growth."
26394157
sparser
"The mTOR associated small G protein Ras homolog enriched in brain (Rheb) is believed to mediate the mTOR-dependent phosphorylation of both 4E-BP1 and p70S6K [148] ."
16297654
reach
"MTOR phosphorylates S6K1 and 4E-BP1, and the most downstream effector, eIF4E."
25884680
rlimsp
"Upon activation by proliferative stimuli mediated by the PI3K/Akt signal transduction pathway, mTOR phosphorylates/activates p70S6K, which in turn phosphorylates the 40S ribosomal protein S6 (20)."
25729255
reach
"Studies have indicated that phosphorylation of p70 S6 kinase and 4EBP1 by mTOR plays an essential role in tumor growth and metastasis [XREF_BIBR - XREF_BIBR]."
26582057
reach
"HCMV infection does not induce mTOR independent phosphorylation of a third mTOR effector, p70S6 kinase (p70S6K)."
15452223
reach
"Upon mitogenic stimuli, mTOR phosphorylates 4E-BP1 and 4E-BP2 and the protein kinases S6K1 and S6K2 (S6Ks)."
25594050
sparser
"Activated mTOR could up-regulate phosphorylation of downstream p70S6K and enhance long-term potentiation by regulating local protein synthesis."
30086367
sparser
"The function of mTOR is to stimulate cell growth through mTOR complex 1 (mTORC1)-mediated phosphorylation of S6K1 and 4E-BP1 ( xref ) and mTOR complex 2 (mTORC2)-mediated phosphorylation of AKT (Ser473) ( xref )."
32174768
reach
"When cells are stimulated by hormones or mitogenic signals, mTOR binds to the EIF3 complex and phosphorylates S6K1."
28854163
sparser
"A recent model proposed by the Blenis laboratory suggests that S6K1 is phosphorylated in the pre-initiation complex by mTOR leading to dissociation and full activation of S6K1 by PDK1."
16953237
reach
"TNFalpha induces mTOR activation, and then mTOR phosphorylates its target S6K1."
26343727
reach
"The involvement of mTOR signaling in fibroblast proliferation is also supported by our finding that FGF2, a growth factor that activates mTOR signaling [55,56], induced the phosphorylation of PS6K and increased fibroblast proliferation.The activation of mTOR signaling induces protein synthesis by translating genes responsible for cell growth and proliferation."
| PMC
reach
"Treatment with EPO leads to mTOR activation and phosphorylation of p70S6K and 4EBP1."
23029019
reach
"Additionally, baicalein negatively regulated the m-TOR signaling pathway involved in lipid accumulation during adipogenesis, thus inhibiting phosphorylation of m-TOR and p70S6K."
24560969
reach
"These pathways can be vital for cell survival since loss of mTOR signaling prevents phosphorylation of both p70S6K and 4EBP1 and results in apoptosis."
22873724
reach
"MTOR associates with Raptor (mTORC1 complex) to phosphorylate p70S6K, which in turn phosphorylates 4E-BP1, leading to increased cell proliferation."
23427297
sparser
"rhArg treatment significantly decreased the protein level of phosphorylated mTOR and phosphorylated p70S6K, which reflected the activity of mTOR, indicating that rhArg inhibited mTOR/S6K signaling pathway during autophagy."
24113174
reach
"When phosphorylated, mTOR will be promoting cell protein synthesis by phosphorylating p70S6K."
29997438
reach
"Raptor is required for mTOR phosphorylation of 4E-BP1 and enhances mTOR phosphorylation of p70S6K."
27054578
reach
"Direct phosphorylation of 4E-BP1 and S6K1 by mTOR, or indirect regulation of phosphorylation by an mTOR regulated kinase or phosphatase, has been suggested [19, 20]."
12747827
sparser
"Upon mitogenic stimuli, mTOR phosphorylates 4E-BP1 and 4E-BP2 and the protein kinases S6K1 and S6K2 (S6Ks)."
25594050
reach
"Finally, only the bacterially expressed form of S6K1 is phosphorylated by mTOR and is not a form expressed in mammalian cells [10 *]."
10072357
sparser
"MTOR phosphorylates p70S6K and creates a docking site for PDK1, which then phosphorylates Thr229 of p70S6K resulting in its activation."
24604594
reach
"The mTOR protein, a highly conserved serine/threonine kinase, positively regulates the phosphorylation of p70S6 kinase, and S6 ribosomal protein is a well-known substrate of p70S6 kinase."
27863387
reach
"MTOR integrates stimulating signals and phosphorylates eukaryotic initiation factor 4E binding protein 1 (4E-BP1) and p70 ribosomal protein S6 kinase (p70S6K) XREF_BIBR, XREF_BIBR."
25048611
reach
"Similarly, silencing mTOR also dramatically blocked the phosphorylation of Akt, mTOR, S6K1, 4E-BP1 and ULK1 in the cells stimulated with and without hsBAFF."
30244168
reach
"To determine whether AV accumulation in the NPC1 mouse brain is caused by an mTOR mediated induction of autophagy, mTOR activity was assessed by measuring the extent of mTOR specific phosphorylation of p70S6 kinase (XREF_FIG, A and C)."
20864542
sparser
"Over-expression of UBR1 or UBR2 resulted in a reduction in mTOR-dependent S6K1 phosphorylation, whereas knockdown of UBR1 or UBR2 increased S6K1 phosphorylation in amino acid-starved human 293T cells."
20298436
sparser
"ROS-triggered PI3K activation can transduce oncogenic AKT-mTOR signaling; mTOR can increase the phosphorylation of both eukaryotic initiation factor 4E-binding protein1 (4EBP-1) and S6K1, leading to the translation and expression of mRNAs encoding several major anti-apoptotic proteins including XIAP, c-IAP1, Bcl-XL, and BCl-2 [ xref ]."
28250388
reach
"MTOR signalling mediates the phosphorylation of eIF4EBP1 and the ribosomal protein p70S6K, thereby affecting the translation of mRNA [49] ."
23552196
reach
"Under non stressed conditions, mTOR phosphorylates p70 S6 kinase (p70S6K) and eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1)."
21750711
reach
"Active mTOR phosphorylates p70S6 kinase and 4E-BP1 permitting mRNA translation."
20186705
sparser
"Under the condition of stimuli, PI3K phosphorylates PIP2 to create PIP3 and recruits AKT to the plasma membrane to active PDK1 and mTOR complex which phosphorylates 4E-BP1 and p70 ribosomal S6 kinase that trigger ribosome biogenesis and translation in cell growth and division [ xref , xref ] ( Figs. xref & xref )."
31988639
sparser
"On the other hand, the constitutive interaction is clearly not required for in vitro kinase activity as we found that raptor-depleted mTOR strongly phosphorylates S6K1."
12150925
reach
"The mammalian target of rapamycin (mTOR), which phosphorylates p70S6K and 4EBP1 and activates the protein translation process, is upregulated in cancers and its activation may be involved in cancer development."
19845851
sparser
"The rapamycin dose most effective in decreasing lytic replication in B cells, without impairing cell growth (5 nM), was also very effective in inhibiting the phosphorylation of p70S6K by mTOR in Raji cells (Figure  xref E, lane 2)."
24917448
rlimsp
"These results suggest that TRAF4 overexpression enhanced the ability of mTOR to interact and phosphorylate p70s6k."
25738361
reach
"As a consequence of this interaction, mTOR and Raptor phosphorylates S6K1, and thus regulates downstream effectors of mTOR and cap dependent translation initiation [XREF_BIBR, XREF_BIBR]."
26497985
reach
"MTOR inhibition with rapamycin at below pharmacological concentrations blocked p70S6K phosphorylation and induced a differentiated contractile phenotype with smooth muscle (sm)-calponin, sm-alpha-actin, and SM protein 22-alpha (SM22alpha) expression."
19074484
reach
"eIF4E binding protein 1 (4E-BP1) and ribosomal protein p70 S6 kinase 1 (p70S6K1) are phosphorylated by mechanistic target of rapamycin complex 1 (mTORC1), which enhances cap-dependent protein translation (196, 197) ."
35422818
sparser
"Activated mTOR phosphorylates its two major targets, S6K1 and 4E-BP1, which play a key role in myogenesis."
27340276
sparser
"Rapamycin forms a complex with FKBP12, which binds mTOR xref , xref and inhibits its activity by causing disassembly of the mTORC1 complex. xref Although rapamycin rapidly inhibits phosphorylation of p70S6K by mTOR, 4E-BP1 phosphorylation slowly decreases within 2 h."
25361081
reach
"MTOR phosphorylates p70 ribosomal S6 kinase (p70S6 kinase) that regulates translation of proteins involved in cellular proliferation and formation."
20576128
rlimsp
"Bupivacaine induced loss of S6K1 phosphorylation at putative mTOR site and at the activation loop as a consequence, without any perceptible change in mTOR kinase activity or expression observed herein, substantiates the later possibility even further."
24605337
reach
"S6K-1, which is phosphorylated and activated by mTOR, in turn, phosphorylates ribosomal protein S6, which is a commonly used readout of mTOR activity."
19553465
reach
"Downstream of AKT is mTOR, a kinase that phosphorylates p70 S6 kinase."
26473529
sparser
"As shown in xref , activated mTOR also phosphorylates the downstream substrate S6K1, which then phosphorylates the IRS protein and induces IRS degradation, resulting in less interaction between IRS with IGF1R and insulin receptors."
27099521
sparser
"In this instance, p70 is phosphorylated by mTOR acting as an ‘ATP sensor’ in response to a rise in β-cell ATP levels caused by increased glucose metabolism ."
12127723
isi
"Increased phosphorylation of p70S6K, a major downstream target of mTOR, and upregulated expression of genes involved in cholesterol biosynthesis are indicative of the key role played by IGF1 in regulating the synthesis of cholesterol, the precursor for steroid hormones."
32586423
reach
"TCS1/2-mediated mTOR activation leads to phosphorylation of its substrate 4E-BP or S6K1, resulting in upregulated translation of a large pool of proteins in the regenerative process, such as GAP-43 [32]."
32629985
reach
"The turn motif phosphorylation helps to dock the HM segment to a hydrophobic pocket in the N-lobe formed by the alphaC helix, and phosphorylation of activation loop results in ordering of the activation segment clearing the way for the mTOR to phosphorylate the HM site of S6K1."
21561857
sparser
"The first mTOR complex, mTORC1, phosphorylates both S6K1 and 4E-BP1 ( xref )."
21952136
reach
"Rapamycin is a specific inhibitor of mTOR signaling that binds directly to the mTOR complex 1 (mTORC1) and suppresses mTOR mediated phosphorylation of S6K1 and 4E-BP1."
24675464
sparser
"P70 S6 kinase, which is phosphorylated by mammalian target of rapamycin complex 1 (mTORC1) downstream of AKT, showed increased phosphorylation levels at ZT 16–22 (Figure xref )."
30210362
sparser
"It is reported that mTOR activation is the mechanism of 5-HT-induced hepatic steatosis and IR mediated by 5-HT 2 R in the liver and adipocytes xref , xref , so we examine LTS or 5-HT-indueced activation of hepatic mTOR and S6K, a downstream target of mTOR, by detecting Ser 2448 phosphorylation of mTOR, and Thr 389/412 phosphorylation of p70S6K."
26884719