IndraLab
Statements
Cyclic peptide inhibits PSMD14. 2 / 2
|
2
reach
"Hence we concluded that among the other members of the UCH family, the cyclic β-sheet peptide 2 inhibits UCH-L5 most efficiently.To further investigate whether the cyclic peptide 2 inhibits the activity of Rpn11, the metalloprotease DUBs found in the 19S cap of the 26S proteasome, we carried out a standard fluorescence polarization assay using a Ubiquitin-Fluorescence Polarization (Ub-FP) substrate containing Ub linked by an isopeptide bond to a TAMRA-labelled Ub peptide, comprising residues 41 to 54 of Ub (Supplementary Figure S3A)."
reach
"We observed that the cyclic peptide, 2, was unable to inhibit Rpn11/Rpn8 activity (Supplementary Figure S3B), potentially suggesting that the Rpn11 activity remains unhindered in cells in the presence of 2.Next, we tested whether our cyclic peptide 2 can compete with the activity-based probe Cy5-UbVME in the labeling of UCH-L5."