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SMAD3 is methylated. 88 / 88
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"Meta-analysis of the association between SMAD3 CpG7 methylation and childhood asthma risk in neonates born to asthmatic mothers was performed by using estimates from each study cohort to compute the[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28011059
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"Allele-specific DNA methylation of SMAD3 is regulated based on genetic effects relevant to disease susceptibility."
33036415
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"We investigated whether SMAD3 methylation is associated with survival in CRC."
33036415
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"Given these findings, the objective of this study was to assess a larger sample to further investigate the relationship between Smad3 gene methylation and pulmonary fibrosis in Uygur PBL patients."
28562330
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"In contrast, asthmatic and non-asthmatic children of non-asthmatic mothers did not differ in their SMAD3 methylation levels [ P for interaction (by linear regression) = 0.001; xref ]."
28011059
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"In contrast, asthmatic and nonasthmatic children of nonasthmatic mothers did not differ in their SMAD3 methylation levels ( P for interaction [by linear regression] = ."
28011059
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"Linear regression was used to test for an interaction between child and maternal asthma on SMAD3 methylation."
28011059
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"SMAD3 methylation in IIS (n = 60) and in 2 replication cohorts (the Manchester Asthma and Allergy Study [n = 30] and the Childhood Origins of Asthma Study [n = 28]) was analyzed by using bisulfite sequencing or Illumina 450K arrays."
28011059
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"Moreover , SMAD3 methylation in IIS neonates with maternal asthma was strongly and positively associated with neonatal production of IL-1β, an innate inflammatory mediator."
28011059
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"Smad3 methylation by EZH2 promotes its activation and tumor metastasis."
35085106
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"Mean SMAD3 methylation levels at birth were 41.3% (95% CI 35.5–47.5) in non-asthmatics and 47.1% (95% CI 40.6–53.1) in asthmatics, a difference that did not reach statistical significance ( P =0.2 by Wilcoxon two-sample test: xref )."
28011059
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"Therefore, we relied again on the IIS to explore the relationship between neonatal LPS-induced IL-1β production, SMAD3 promoter methylation and childhood asthma."
28011059
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"SMAD3 methylation estimates from bisulfite sequencing and microarrays were also highly correlated (ρ = 0.46, P  = ."
28011059
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"Our results unveil that the histotype-specific regulation of tumor fibrosis in lung cancer is mediated through differential SMAD3 promoter methylation in TAFs, and provide new mechanistic insights on the selective poor response of SCC-TAFs to nintedanib."
31694906
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"Smad3 methylation, Smad3 mRNA expression and TGF-β level were correlated with the number of pigeons bred by patients."
28562330
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"A meta-analysis revealed that for each 10% increase in SMAD3 CpG7 methylation there is nearly a two-fold increased risk of childhood asthma (meta-analysis Odds Ratio=1.95, [95%CI: 1.23, 3.10], P =0.005; heterogeneity P =0.5)."
28011059
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"Importantly, the finding that SMAD3 methylation patterns are increased at birth in children who develop asthma at age 9 suggests that progression towards asthma may begin in utero [ xref ]."
32887352
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"SMAD3 methylation in IIS (n=60) and in two replication cohorts (The Manchester Asthma and Allergy Study, n=30, and the Childhood Origins of ASThma Study, n=28) was analyzed by bisulfite sequencing or Illumina 450K arrays."
28011059
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"EZH2-mediated SMAD3 K53/K333 methylation is upregulated and correlated with SMAD3 hyperactivation in breast cancer, promotes tumor metastasis, and is predictive of poor survival outcome."
35085106
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"Dimopoulos and colleagues showed that acquired IMiD resistance is associated with an increase in genome-wide DNA methylation and downregulation of SMAD3."
30555699
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"We used two TAT-peptides to abrogate SMAD3 methylation and therapeutically inhibit cancer metastasis."
35085106
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"While it is unclear whether these mechanisms operate pre- and/or perinatally, detection of the relationship between SMAD3 methylation and IL-1β production selectively among children of asthmatic mothers implies that the in utero environment is critical for directing the epigenetic trajectory towards childhood asthma."
28011059
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"In another study on Chinese Uygur population by the same group, methylation of the gene Smad3 is reported."
34498177
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"In contrast, the children of asthmatic mothers who developed asthma were almost entirely found among those with high SMAD3 promoter methylation and high IL-1β secretion ( P =0.009 by Fisher’s Exact Test; xref )."
28011059
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"Mean SMAD3 methylation levels at birth were 41.3% (95% CI, 35.5% to 47.5%) in nonasthmatic subjects and 47.1% (95% CI, 40.6% to 53.1%) in asthmatic subjects, a difference that did not reach statisti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28011059
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"Although associations between SMAD3 variants [rs17228058 ( xref ), rs744910 ( xref ), rs17294280 ( xref )] and asthma have been reported in GWAS, asthma-related SMAD3 methylation differences were unlikely to be influenced by SMAD3 genotype."
28011059
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"Mechanistically, EZH2-triggered SMAD3 methylation facilitates SMAD3 interaction with its cellular membrane localization molecule (SARA), which in turn sustains SMAD3 phosphorylation by the TGFB receptor."
35085106
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"Notably, we also reported that stimulating normal pulmonary fibroblasts with cigarette smoke condensate in culture was sufficient to time- and dose-dependently increase the promoter methylation of SMAD3 but not that of its closely related homolog SMAD2 [ xref ], as summarized in xref A–C. Although the underlying mechanisms of the selective SMAD3 hypermethylation remains unclear, it is conceivable that the common DNMT1 increase in smokers may be involved."
34359678
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"Interestingly, we observed that both SMAD2 and SMAD3 were differentially methylated in OA patients compared to controls, indicating that epigenetics is a driving force in this shift."
24838673
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"We investigated the relationship between Smad3 gene methylation and pulmonary fibrosis in pigeon breeder's lung (PBL)."
28562330
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"Unlike EDARADD , no prognostic information was observed in SMAD3 or CHI3L1 methylation status in the same data sets (, available at Carcinogenesis Online)."
26449251
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"These results suggest that a strong relationship exists between neonatal SMAD3 methylation, production of the innate cytokine IL-1β, and childhood asthma."
28011059
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"Our study found that Smad3 was involved in the development of pulmonary fibrosis in patients with PBL and that Smad3 gene methylation was the cause of abnormal Smad3 mRNA expression."
28562330
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"Correlation between Prognosis and SMAD3 Methylation Level."
33036415
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"It is reported that the changes of the methylation of SMAD3 and SMAD6 were correlated with gene transcription levels in CKD samples [ xref ]."
34462056
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"Of the large number of DNA methylation changes they identified, increased methylation in SMAD3 was: (1) associated with childhood asthma risk, a result that was replicated in two independent cohorts; (2) was the most connected node within the network of asthma-associated DMRs; and (3) was strongly and positively associated with neonatal production of IL-1β, an innate inflammatory mediator."
29389731
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"In contrast, the children of asthmatic mothers who had asthma were almost entirely found among those with high SMAD3 promoter methylation and high IL-1β secretion ( P  = ."
28011059
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"Thus, circulating methylated SMAD3 cannot distinguish patients from healthy participants in a plasma volume of 200 μL. As evidenced by previous studies, the column-based method may lead to cross-contamination [ xref , xref ]."
33036415
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"Finally, we asked whether the SMAD3 methylation levels measured at birth in children of asthmatic mothers are associated with risk for childhood asthma in our three cohorts."
28011059
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"Most importantly, the SMAD3 methylation differences detected between asthmatic and non-asthmatic children of asthmatic mothers remained significant ( P =0.04 by Wilcoxon two-sample test) even after adjusting for CBMC composition ( xref )."
28011059
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"Bisulfite sequencing of cord blood cell DNA from our IIS neonates and two replication cohorts (The Manchester Asthma and Allergy Study, and the Childhood Origins of ASThma Study) showed that SMAD3 promoter methylation at birth was selectively and significantly increased in asthmatic children of asthmatic mothers and was associated with childhood asthma risk."
29709672
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"Of interest, SMAD3 DNA methylation has also been associated with atopic asthma (at age 5 years) in a study of children with a history of severe adenovirus-induced wheezing in infancy ( xref )."
31001502
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"The correlation between pulmonary fibrosis and methylation of peripheral Smad3 in cases of pigeon breeder's lung in a Chinese Uygur population."
28562330
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"Although it is unclear whether these mechanisms operate prenatally and/or perinatally, detection of the relationship between SMAD3 methylation and IL-1β production selectively among children of asth[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28011059
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"Finally, we asked whether the SMAD3 methylation levels measured at birth in children of asthmatic mothers are associated with risk for childhood asthma in our 3 cohorts."
28011059
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"In all 3 cohorts methylation in SMAD3, the most connected node within the network of asthma-associated, differentially methylated regions, was selectively increased in asthmatic children of asthmatic mothers and was associated with childhood asthma risk."
28011059
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"In addition, combining SMAD3 with other hypomethylated genes, such as ACOT7 , RASA3 , B3GNTL1 , PIGB , MAP3K5 , LPIN1 , and MYBPC3 , which are potential biomarkers of CRC in the analytical model, may improve accuracy, increase sensitivity, and reduce false-positive results when predicting CRC in circulating methylated SMAD3 ."
33036415
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"Mechanistically, the reduced fibrosis and nintedanib response of SCC-TAFs were associated with increased promoter methylation of the pro-fibrotic TGF-β transcription factor SMAD3 compared to ADC-TAFs, which elicited a compensatory increase in TGF-β1/SMAD2 activation."
31694906
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"In particular, DNAJA4 , TFR2 , SMAD3 , PLAG1 , FGF1 and HNF4A methylation represent novel and possibly physiologically relevant markers of neonatal adiposity."
31851703
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"19-21 Therefore we relied again on the IIS to explore the relationship between neonatal LPS-induced IL-1β production, SMAD3 promoter methylation, and childhood asthma."
28011059
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"No study has focused on the relationship between the SMAD3 methylation level and CRC diagnosis."
33036415
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"Fetal epigenetic regulation is modifiable by the maternal environment and has been proposed as a mechanism by which prenatal environmental stimuli contribute to the development of respiratory disorders. xref In support of this notion, mechanistic animal studies have demonstrated that maternal microbial exposures, through DNA methylation and histone modifications, may modulate the expression of important immune factors such as interleukin (IL-4) and interferon-γ genes in the offspring’s lungs. xref , xref Increases in histone H3 acetylation linked to an asthma phenotype have been also identified in mice pups after maternal nicotine exposure. xref In humans, epigenetic modifications (e.g., SMAD3 methylation) have been proposed as a mechanism mediating the intergenerational risk of asthma in children from asthmatic mothers. xref Furthermore, epigenetic modifications may also mediate the effect of noninheritable prenatal factors on respiratory outcomes."
34388306
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"Collectively, these findings reveal the complicated layers in regulation of SMAD3 activation coordinated by EZH2-mediated SMAD3 K53/K333 methylation to drive cancer metastasis."
35085106
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"Previously, we conducted a preliminary screening analysis of whole-genome methylation in 4 cases of Uygur PBL and 4 Uygur pigeon breeders without this disease using the Illumina 450K method (Illumina 450K Infinium Methylation BeadChip; SHBIO, Shanghai, China), and the results showed abnormal methylation of the Smad3 gene among the Uygur PBL patients."
28562330
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"DNA methylation of SMAD3 was analyzed in 38 paired colorectal samples and 314 tumor sample datasets from TCGA."
33036415
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"When the relation between SMAD3 methylation at birth and childhood asthma was examined separately in children with and without maternal asthma, SMAD3 methylation was found to be significantly incr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28011059
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"We demonstrated that PRMT5-overexpression enhanced methylated-Smad3 levels in vivo and in vitro."
36056082
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"Maternal asthma modifies the relation between neonatal SMAD3 methylation and IL-1ββ β producing capacity."
28011059
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"The distributions of SMAD3 methylation and log IL-1β production in non-asthmatic and asthmatic children without and with a maternal history of asthma were compared by dividing mean percentage SMAD3 methylation and log IL-1β secretion at the median, thereby creating four groups (low/low, low/high, high/low and high/high)."
28011059
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"In the latter study, differential methylation of SMAD3 (SMAD family member 3) was found to be particularly associated with asthma [ xref ]."
| PMC
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"The level of Smad3 gene methylation, mRNA expression, and the extent of pulmonary fibrosis in patients with PBL were analyzed based on Pearson correlation coefficients."
28562330
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"Previous studies, both in China and in other countries, have mainly focused on the relationship between Smad3 and fibrosis, and no prior investigation has reported the abnormal expression of Smad3 with respect to methylation of the Smad3 gene."
28562330
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"Smad3 methylation."
28562330
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"However, the role of SMAD3 methylation in cancer is still unclear."
33036415
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"In conclusion, SMAD3 methylation levels vary in colorectal tissue based on the type of cancer."
33036415
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"While the Hdac4 methylation localized within CCWGG, the Smad3 and Mid1 methylations were present at CCGG motifs within intronic enhancers."
32926553
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"Most importantly, the SMAD3 methylation differences detected between asthmatic and nonasthmatic children of asthmatic mothers remained significant ( P  = ."
28011059
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"The distributions of SMAD3 methylation and log IL-1β production in nonasthmatic and asthmatic children without and with a maternal history of asthma were compared by dividing mean percentage SMAD3 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28011059
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"Pathologically, EZH2 expression increasing results in the accumulation of SMAD3 methylation to facilitate SMAD3 activation."
35085106
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"The SMAD3 methylation level in CRC tumors, which was half that of adjacent normal colorectal tissue, was defined as hypomethylation."
33036415
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"When the relation between SMAD3 methylation at birth and childhood asthma was examined separately in children with and without maternal asthma, SMAD3 methylation was found to be significantly increased in asthmatic compared to non-asthmatic children of asthmatic mothers ( P =0.005 by Wilcoxon two-sample test)."
28011059
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"Correlations of Smad3 gene methylation and Smad3 mRNA expression with the extent of pulmonary fibrosis."
28562330
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"A meta-analysis revealed that for each 10% increase in SMAD3 CpG7 methylation, there is a nearly 2-fold increased risk of childhood asthma (meta-analysis odds ratio, 1.95; 95% CI, 1.23-3.10; P  = ."
28011059
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"Therefore, Smad3 gene hypomethylation may be caused by long-term exposure to allergen sources; an increase in levels of TGF-beta, an upstream factor of Smad3; or interactions between these two factors."
28562330
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"Augmented methylation of SMAD3 in cord blood mononuclear cells of newborns, which was associated with the inflammatory regulator IL-1b, determined whether or not the subjects, all with asthmatic mothers, would develop asthma by age 9 [ xref ]."
33781317
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"In children born to asthmatic mothers, SMAD3 methylation at birth was associated with childhood asthma risk and was more prominent in asthmatic mothers [ xref ]."
31776749
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"We stratified the overall survival of Taiwanese patients with CRC and patients in TCGA datasets into two subsets on the basis of SMAD3 methylation: low hypomethylation and no-hypomethylation groups."
33036415
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"Our results also support that smoking and/or the anatomic location of SCC in the proximal airways, which are more exposed to cigarette smoke particles, may prime SCC-TAFs to stronger SMAD3 epigenetic repression, since cigarette smoke condensate selectively increased SMAD3 promoter methylation."
31694906
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"After constructing a genomic methylation analysis for patients with PBL and detecting abnormal Smad3 gene hypomethylation, we hypothesized that pulmonary fibrosis in patients with PBL is correlated with Smad3 gene methylation."
28562330
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"IL-1β concentrations were measured by means of ELISA (Quantikine; R&D Systems, Minneapolis, Minn) in supernatants of LPS-stimulated IIS CBMCs (n = 57) also tested for SMAD3 promoter methylation."
28011059
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"Although associations between SMAD3 variants (rs17228058, 25 rs744910, 23 and rs172942[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28011059
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"Smad3 gene methylation and Smad3 mRNA expression were negatively correlated, with a correlation coefficient of -0.84."
28562330
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"In all three cohorts, methylation in SMAD3 , the most connected node within the network of asthma-associated DMRs, was selectively increased in asthmatic children of asthmatic mothers and was associated with childhood asthma risk."
28011059
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"Comparable results were obtained by assessing SMAD3 methylation in 28 COAST neonates with the Illumina Infinium HumanMethylation450 BeadChip array."
28011059
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"Importantly, the finding that SMAD3 methylation patterns are increased at birth in children who develop asthma at age 9 suggests that progression towards asthma may begin in utero [58]."
32887352
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"The cis-acting element methylation of HDAC4, SMAD3, and MID1 may play a regulatory role during palatal fusion."
30277813
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"DNA methylation in cord blood mononuclear cells from the participants of the Infant Immune Study identified SMAD3 methylation as associated with asthmatic children whose mothers were also asthmatic [ xref ]."
35055381
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"Pretreatment with a specific PRMT5 inhibitor EPZ015666 (5 nM) or overexpression of a catalytically inactive mutant of PRMT5, PRMT5(E444Q), reduced PRMT5-induced methylation of Smad3, thus suppressing PRMT5-mediated cardiac fibroblast activation in vitro."
36056082