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PSMD14 increases the amount of ESR. 8 / 8
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"Inhibition of ERα degradation using the proteasome inhibitor MG132 showed that PSMD14 depletion decreases ERα protein level, and this effect can be reduced by MG132 treatment in MCF-7 cells (Fig. 5H, I)."
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"Moreover, volcano plot revealed that PSMD14 depletion effectively suppressed the expression of classical ERα target genes, such as TFF1, PKIB, and CCND1 (Fig. 3D)."
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"The data showed that PSMD14 depletion not only decreased ER expression, but also decrease PR expression in both mRNA level and protein level (Fig. S3B–D)."
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"Additionally, qRT-PCR data revealed that PSMD14 silencing inhibited the expression of ERα target genes, such as GREB1, TFF1 and IL20, in both vehicle and estradiol-treated conditions (Fig. 4I, J)."
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"Conversely, PSMD14 overexpression in MCF-7 cells increased the levels of ERα protein, ERα signaling activity, and the expression of ERα target genes, such as GREB1, TFF1 and IL20 (Fig. 4K, L)."
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"qRT-PCR data also revealed that pharmaceutical inhibition of PSMD14 could suppress the expression of ERα target genes, such as GREB1, TFF1 and IL20, in both MCF-7 and T47D cells (Fig. 8B, Fig S2B)."
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"Furthermore, when treated with estradiol, PSMD14 depletion inhibited ERα protein levels in both vehicle and estradiol-treated conditions (Fig. 4E, F)."
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"PSMD14 depletion in MCF-7 and T47D cells decreased the levels of ERα protein, but had no significant effect on the mRNA levels of ERα (Fig. 4A–D)."
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