IndraLab
Statements
reach
"Analysis
of the dose-dependent antagonism of both HDAC6 and USP16 suggests
that using 25 at a concentration of 3 μM would
limit the off-target effects of USP16 while achieving significant
antagonism of HDAC6.To further characterize the selectivity of 25, we
synthesized 33 (Figure 5c(i)), a biotin-labeled derivative of 25, for use as an affinity reagent for proteome-wide cellular target
engagement profiling."