IndraLab

Statements

SCN5A binds NUP107. 7 / 7
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"UsingRIP‐PCRand luciferase assay, we found that the 5′‐UTRof Scn5a mRNAwas not involved in the interaction, whereas the spatial interaction between Nup107 protein and Scn5a mRNAwas formed when Scn5a mRNApassing through the nuclear pore."
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"Furthermore, the luminescent reporters containing subcloning segments of CDS and 3′‐UTR of Scn5a mRNA exhibited the similar results to their corresponding full‐length reporters (Figure  xref D), indicating that the regions of CDS and 3′‐UTR of Scn5a mRNA, were critical for the Nup107‐mediated Scn5a mRNA export, whereas the 5′‐UTR seemed dispensable for the interaction of Scn5a mRNA with Nup107."
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"Unexpectedly no special domain of Nup107 was found to be critical for the Nup107 protein‐ Scn5a mRNA interaction (Figure  xref E and F)."
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"The spatial interaction between Nup107 protein and Scn5a mRNA is implicated in the regulation of Scn5a mRNA export."
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"The spatial interaction between Nup107 protein and Scn5a mRNA is critically required for Scn5a posttranscriptional regulation in cardiomyocytes."
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"Given that Scn5a mRNA forms a three‐dimensional structure before its nuclear export, it is understandable that this conformation of Scn5a mRNA is induced by the flexible Nup107, and consequently the interaction between Nup107 protein and Scn5a mRNA is dynamically changed in the process of mRNA passing through the nuclear pore."
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"Second, active interaction between Nup107 protein and Scn5a mRNA is required for the Scn5a mRNA export and subsequent translation."
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