IndraLab
Statements
sparser
"Similarly, when a large portion of nociceptive neurons were depleted using Nav1.8-Cre lineage ablation in mice, monocyte recruitment and lymphadenopathy increased in a Staphylococcus aureus subcutaneous infection model; CGRP also decreased TNFα production from macrophages ( xref ) [ xref ]."
sparser
"Although it is possible that Cre expression in dorsal root ganglia in the Nav1.8-Cre mice may not be restricted to nociceptors and may also affect low threshold mechanoreceptor sensory neurons ( xref ), it remains unclear whether deletion of DORs solely on nociceptors is responsible for the enhancement of mechanical pain."
sparser
"Viewed with TEM, the ultrastructure of pathological Nav1.8 Cre/+ ;ROSA26 lacZ /+afferents ( xref ) was similar to those of theKcna6 lacZ/lacZ mice ( xref ), while normal morphology of primary afferent terminals was observed in Nav1.8-Cre negative littermate controls ( xref ) and in Kcna6 -/- knock-out mice not expressing lacZ ( xref )."
sparser
"In Nav1.8-ChR2 transgenic mice constructed by knocking the ChR2 gene into Nav1.8-cre mice, remote or epidural blue light illumination induces the activation of sensory neurons and central sensitization in the spinal cord, as well as mechanical hypersensitivity, avoidance behavior, and conditioned place aversion in animals [ xref , xref ]."
sparser
"By crossing Meis1 F mice with transgenic Nav1.8-Cre mice, in which Cre recombinase is driven from a genomic fragment derived from the Nav1.8 promoter region and is selectively expressed in most nociceptive neurons, we generated Meis1 conditional knockout mice (referred to as Meis1cko )."
sparser
"Whole-mount immunostaining of ing-scWAT from sensory nerve reporter mice ( Nav1.8-Cre x TdTomato ) validated the presence of small, distinct sensory axons innervating adipocytes in the parenchyma, and also supported earlier findings of sensory fibers within larger nerve bundles in ing-scWAT."
sparser
"To investigate whether activating nociceptors is sufficient to promote mucus growth, we bred Nav1.8-Cre mice with hM3Dq reporter mice to drive expression of both mCitrine and hM3Dq, a designer receptor exclusively activated by designer drugs (DREADD) whose ligand is clozapine N-oxide (CNO) (Nav1.8 hM3Dq ) ( xref ) ( xref )."
sparser
"Although further studies are warranted to investigate whether the residual TRPV4 in sensory neurons (~20%), most likely outside of Nav1.8-cre + neuronal population, plays a functional role in CGRP release, it is plausible that deletion of Trpv4 from Nav1.8-cre + neurons can have a dramatic effect on secretion of CGRP because CGRP is exclusively expressed within a subset of Nav1.8-cre + neurons xref ."
sparser
"To explore the role of Nav1.8+ sensory nerves within the tumour microenvironment, we have induced targeted diphtheria toxin‐based cell ablation. xref We crossed Nav1.8‐Cre mice with inducible diphtheria toxin A (iDTA) transgenic mice to specifically deplete all sensory neurons. xref Nav1.8‐Cre/iDTA mice were previously shown to be devoid of all Nav1.8‐expressing nociceptors, xref and have no response to mechanical stimuli, noxious heat or capsaicin. xref Genetic depletion of sensory nerves was confirmed by immunohistochemistry to Nav1.8 in the dorsal root ganglions of these animals (Figure xref C,D)."
sparser
"In a lethal model of Staphylococcus aureus pneumonia ( xref ), depletion of TRPV1+ nociceptive neurons through Trpv1-Dtr, Nav1.8-cre;Dta or resiniferatoxin administration significantly improves survival and bacterial clearance, demonstrating nociceptor suppression of protective immunity."
sparser
"On the other hand, in Nav1.8-Arch mice, obtained with heterozygous Nav1.8-Cre mice crossed with homozygous Ai35 mice carrying the floxed stop-Arch-EGFP gene in the ROSA26, the mechanical and thermal sensitization was completely prevented with the stimulation of yellow light ( xref )."
sparser
"Interestingly, Nav1.8-Cre based Toll-like receptor 4 (TLR4) deletion attenuates acutely (day 1–5 post SNI) but not chronically (day 7 post SNI) the mechanical hypersensitivity in female mice, but has no effects on mechanical pain in male mice; conversely, TLR4 conditional deletion reduces cold allodynia up to day 7 post SNI as measured in male mice but not in female mice [ xref ]."
sparser
"As mentioned above, early mortality in Nav1.8-Cre Rosa26DTA nociceptor-deficient mice during LPS-induced shock has been attributed to increased brain QUIN levels, and mortality in these mice is ameliorated by the central administration of an IDO1 inhibitor, suggesting that the peripheral influx of QUIN and other KYN metabolites into the brain during sepsis has a negligible effect on survival [ xref ] ( xref )."
sparser
"Cold allodynia but not mechanical allodynia induced by pT-ION or by virus-mediated overexpression of Cx36 in the trigeminal ganglion was reversed by the GluK2 antagonist NS102, and knocking down Cx36 expression in Nav1.8-expressing nociceptors by injecting virus into the orofacial skin area of Nav1.8-Cre mice attenuated cold allodynia but not mechanical allodynia."
sparser
"To determine novel bacterial mechanisms which may modulate pain-related signaling, we mined our mouse transcriptional dataset of FACS-sorted DRG neuron populations xref and found that Antxr2 was enriched by 5-fold in Na v 1.8 lineage ( Nav1.8-cre Rosa26-Tdtomato +) neurons compared to Parvalbumin lineage ( Pvalb-cre Rosa26-Tdtomato +) proprioceptive neurons ( xref )."
sparser
"The withdrawal responses or avoidance behavior were therefore interpreted as nociceptive responses, which agrees with other studies using optogenetic transgenic mice, where channelrhodopsin was directed to peripheral nociceptive neurons via Nav1.8-Cre [ xref ], also known as SNS-Cre [ xref ] (encoded by Scn10a ), or via transient receptor potential, TRPV1-Cre [ xref ]."
sparser
"After 14 days post pulp exposure (infection), there were no differences in tumor necrosis factor alpha (TNF⍺) gene expression in the periapical lesion between Nav1.8-Cre and Nav1.8-DTA mice with both showing approximately a five-fold increase compared to healthy tissues ( p = 0.35) (Fig. xref c) ( n = 3 per group)."
sparser
"Analysis of gene expression by RNAscope within the periapical lesion demonstrated that there is greater expression of Runx2 mRNA (expressed in osteoblasts) in Nav1.8-DTA mice 3 days following pulp exposure compared to Nav1.8-Cre ( p = 0.01), whereas there is not a significant difference at 7 days ( p = 0.46) (Fig. xref d, f) ( n = 8–11 per group)."
sparser
"This inhibition is further and significantly greater when osteoblasts were co-cultured with primary trigeminal cultures from Nav1.8-DTA mice (27 ± 6.8% of control; p = 0.04) (Fig. xref a, b), whereas there were no significant differences between Nav1.8-cre and Nav1.8-DTA TG culture for Alizarin Red staining ( p = 0.88) (Fig. xref c, d)."
sparser
"Co-culture of murine osteoclast precursor cells (RAW264.7) with primary TG neurons inhibited resorption activities compared to control (67 ± 6.1% of control; p < 0.001) This inhibition was partially reversed by co-culture with neurons from Nav1.8-DTA mice (85 ± 10% of control; p < 0.01) but was still lower compared to Nav1.8-Cre ( p = 0.04) (Fig. xref g) ( n = 6–7 per group)."
sparser
"A previous study, also using Nav1.8-Cre;tdTomato mice, found no difference in innervation density at 14-days after excision of only the extraorbital lacrimal gland ( xref ), indicating that the severity of dry eye likely contributed to the amount of denervation observed in the present study."
sparser
"The use of the Nav1.8-Cre;tdTomato reporter appeared to label the vast majority of corneal afferents, with the likely exception of autonomic neurons and up to 80% of cool sensing neurons that do not express Nav1.8, indicating that use of this mouse could be a valuable tool in longitudinal studies to track corneal sensory innervation ( xref ; xref )."
sparser
"To examine corneal innervation, the Nav1.8-cre;tdTomato reporter mouse line was utilized, since the voltage-gated sodium channel Nav1.8 is preferentially expressed in C-fibers, including > 90% of IB4-binding neurons (nonpeptidergic C-fibers) and CGRP-positive neurons (peptidergic C-fibers) xref ."