IndraLab
Statements
reach
"Chromatin IP (ChIP)-quantitative polymerase chain reaction (qPCR) assays confirmed that Bach1 occupied the Usp7 promoter (XREF_FIG), indicating that Usp7 is a direct target of Bach1, and when Usp7 activity was down-regulated via transfection with small interfering RNAs (siRNAs) and/or treatment with the Usp7 inhibitor P5091, Nanog ubiquitination increased, pluripotency protein levels declined in WT hESCs, and the increase in pluripotency protein levels associated with Bach1 overexpression was abolished (XREF_FIG and fig."