IndraLab

Statements

PSMD14 binds Flag. 8 / 8
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"Furthermore, dose-dependent expression of Flag-PSMD14 in HCT116 cells increased expression of the ALK2 protein ( xref d)."
31685442
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"To further confirm these results, we examined the half-life of the ALK2 protein in the absence or presence of PSMD14 upon treatment of protein synthesis inhibitor cycloheximide (CHX) after a plasmid encoding HA-ALK2 protein was transfected into HCT116 cells together with or without Flag-PSMD14."
31685442
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"Flag-PSMD14 or the corresponding flag-NC plasmids were transfected into UMUC3 cells, and magnetic beads precoated with anti-flag antibodies were used to precipitate PSMD14-conjugated proteins ( xref A)."
40121994
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"For the in vivo deubiquitinating assay, Flag-POH1, V5-MYC and HA-Ubexpressing plasmids were cotransfected into 293T cells using Lipofectamine 3000."
37844756
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"Adenovirus-expressing vector and PSMD14-Flag (containing three Flag tags) were purchased from Vigene Biosciences Corporation (Jinan; Shandong Province, China)."
31634528
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"The cells were infected with adenovirus-expressing vector or PSMD14-Flag."
31634528
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"In brief, the protein complexes containing POH1-Flag were purified by anti-Flag antibody-conjugated resins, and the precipitated proteins eluted by 3 × Flag peptides were subjected to SDS-PAGE segregation and identification analyses ( xref a, Fig. S3c and Table S6)."
30745168
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"To identify which polyubiquitination pattern of ALK2 is deubiquitinated by PSMD14, wild-type ubiquitin (HA-Ubi), the K48 ubiquitin mutant (HA-Ubi-K48) in which six lysine residues except for lysine 48 are substituted into arginines, and the K63 ubiquitin mutant (HA-Ubi-K63) in which only lysine 63 is left intact, were transfected into HCT116 cells with or without Flag-PSMD14."
31685442