IndraLab

Statements

CSN5i-3 binds PSMD14. 6 / 6
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reach
"Binding mode of CSN5i-3 in the CSN5 monomer is known (Schlierf et al., 2016) hence we used the co-crystalized conformation of CSN5i-3 to generate CSN5i-3 bound CSN5-CSN6 heterodimeric, Rpn11 monomeric and Rpn8-Rpn11 heterodimeric complexes by manual docking (structural superimposition)."
30356695
reach
"The CSN5i-3-Rpn11 complex showed a very high Cα-RMSD (Figure 7A) suggesting that Rpn11 undergoes large conformational changes (Figure 9B)."
30356695
reach
"In CSN5i-3-Rpn11 complex, we observed that CSN5i-3 was stable (Figure 7C)."
30356695
reach
"This simulated binding of CSN5i-3 to Rpn11 would not be in agreement with experiment and explain its low inhibitor activity."
30356695
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"We see that binding of CSN5i-3 to Rpn11 is significantly influenced by the presence of Rpn8 (Figures 7C,D, 8B) and the consideration of this heterodimeric state off Rpn11-Rpn8 is necessary to explain capzimin binding and CSN5i-3 non-binding."
30356695
reach
"Therefore, binding of capzimin and CSN5i-3 to the monomeric Rpn11 are physiologically possible."
30356695