IndraLab

Statements

USP46 activates Ubiquitin. 2 / 2
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"For example, in contrast to the USP46 BL1 residue F258, which supports the tail of Ub, the corresponding USP12 residue F262 is pushed into the catalytic cleft and would collide with the substrate.Using ubiquitin-7-amido-4-methylcoumarin (Ub-AMC) as a substrate, we have previously shown that UAF1 activates USP1 mainly by increasing its catalytic turnover (k cat ), instead of its affinity toward the substrate (K M ) (Cohn et al., 2007) ."
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"Initially, we hypothesized that mHTT was a substrate of Usp12 deubiquitinating activity, which could be a potential mechanism for regulating stability, localization, or other ubiquitin related signaling activity of ubiquitin conjugated mHTT."
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