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EGF phosphorylates EGFR. 1000 / 1128
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sparser
"We have previously reported that CXCR7 (independent of binding its ligand, stromal cell-derived factor 1α [SDF-1α]) interacts with the epidermal growth factor receptor (EGFR), leading to increased EGF-stimulated EGFR phosphorylation (particularly at tyrosine 1110 [Y1110]), enhanced downstream mitogenic signaling as well as tumor cell proliferation and survival xref , xref ."
sparser
"In addition, both increased intracellular zinc levels further promoted EGF-induced EGFR phosphorylation and internalisation. (4) Conclusion: This report confirms a transactivating effect of zinc on the EGFR for A549 cells and is the first describing an influence of zinc on the EGFR endocytosis."
reach
"Notably, despite the antibody inhibited EGF stimulated phosphorylation of EGFR in both MDA-MB-231 and BT-549 cells (XREF_SUPPLEMENTARY), it did not cause an appreciable inhibition of cell adhesion to vitronectin (XREF_FIG) even at a concentration (100mug/ml) higher than steady state plasma levels in cancer patients administered with the clinical dosing regimen XREF_BIBR."
sparser
"Moreover, activation of EGFR by EGF increased COX-2 promoter activity and protein expression in Hep3B and Huh-7 cells, whereas blocking PGE(2) synthesis or EP(1) attenuated EGFR phosphorylation induced by EGF, suggesting that the COX-2/PGE(2)/EP(1) pathway also modulate the activation of EGFR by its cognate ligand."
sparser
"Suppressing FUT1/4 expression blocked EGF-induced tyrosine phosphorylation of EGFR and MAPK and inhibited cancer growth ( xref ); Yang et al ( xref ) reported that FUT4 overexpression inhibited cyclophosphamide-induced apoptosis through activating the ERK/MAPK and PI3K/Akt signaling pathways."
sparser
"Treatment with Iressa elicited similar decreases in IL-17E- and EGF-induced Y1086-EGFR phosphorylation in the three cell lines (Figure xref ) and markedly decreased EGFR tyrosine phosphorylation induced by the combination of IL-17E and EGF in BT20 and MDA-MB468 cells and, to a lesser extent, in IJG-1731 cells (Figure xref )."
sparser
"Three lines of evidence suggest the activation of a protein tyrosine phosphatase (PTP) by bradykinin: (i) treatment of A431 cells with bradykinin decreases both basal and EGF-induced EGFR tyrosine phosphorylation, (ii) this effect of bradykinin can be blocked by two different PTP inhibitors, and (iii) bradykinin significantly increased the PTP activity in total A431 cell lysates when measured in vitro."
sparser
"Dominant negative blockade of EGFR inhibits both IR- and EGF-induced EGFR phosphorylation and, in response to repeated dosages of IR, impedes proliferation of mammary carcinoma cells in culture ( xref ) as well as conferring radiosensitivity to xenograft tumors in mice as measured using an ex vivo colony formation assay ( xref )."
reach
"In the present study, we determined that miR-10b enhanced EGF induced invasion, EGFR phosphorylation and extracellular signal regulated kinase 1 (ERK1)/2 activation in PCCs, whereas attenuating EGFR degradation and that these effects were due to miR-10b-induced downregulation of Tat interacting protein 30 (TIP30)."
sparser
"EGF strongly induced EGFR phosphorylation (at Tyr1073) early after stimulation (from 15 minutes to 1 hour), whereas AREG activated EGFR at this phosphorylation site only at the later time-points (from day 7 to day 14), when the effect of EGF on EGFR activation was no longer significant ( xref ; xref for quantification data)."
sparser
"DHW extract or BC mix also failed to prevent EGF-stimulated EGFR phosphorylation ( xref , 1 st panel from top, lane 6 and 10 compared to lane 7), while both inhibited EGFR phosphorylation in cells cultured in full serum ( xref , 1 st panel from top, lane 1and 5 compared to lane 2)."
sparser
"EGF-induced EGF receptor phosphorylation was similar in cells infected with either WT or mutant Salmonella, and neither WT nor sigD(-) Salmonella altered recruitment of the p85 subunit of phosphatidylinositol 3-kinase to EGF receptor, implying that SigD acts downstream of these signaling events."
sparser
"Capsaicin suppressed EGF-induced c-Jun and c-Fos nuclear translocation, and also abrogated the EGF-induced phosphorylation of EGF receptor (EGFR), focal adhesion kinase (FAK), protein kinase C (PKC), phosphatidylinositol 3-Kinase (PI3K)/Akt, extracellular regulated kinase (ERK)1/2, and JNK1/2, an upstream modulator of AP-1."
reach
"Ligand induced multiple phosphorylations of receptor tyrosine kinases are widely believed to occur, based on the results of ensemble biochemical assays; XREF_BIBR - XREF_BIBR however, our recently published results call into question the prevalent model that EGF induces multiple phosphorylations of EGFR on the cell membrane."
sparser
"In KATO-III, a number of tyrosine phosphorylated proteins, including extracellular signal-regulated protein kinases (ERKs) and epidermal growth factor receptor (EGFR), were observed irrespective of EGF stimulation and induction of ERK activity and EGFR tyrosine phosphorylation by EGF were less than that in MKN7."
reach
"We also found evidence for the involvement of ligand shedding in the transactivation of EGFR after PGE 2 stimulation, since pretreatment of the cells with the metalloproteinase inhibitor GM6001 almost completely prevented PGE 2 -induced, but not EGF induced, phosphorylation of EGFR, Akt and ERK."
sparser
"By contrast, bacterial infection induced tyrosyl phosphorylation of ErbB2, which could be prevented by AG1478 treatment ( xref ), indicating that N. meningitidis leads to the selective activation of ErbB2, whereas EGF stimulated both EGFR and ErbB2 phosphorylation, consistent with EGFR/ErbB2 heterodimerization ( xref C)."
reach
"Tyrphostin AG1478, the EGFR inhibitor, inhibits fMLP and NGF associated ROS production, fMLP associated CD11b upregulation and NGF induced TrkA phosphorylation; K252a, the NGF receptor inhibitor, inhibits fMLP or EGF associated ROS production, CD11b expression and EGF induced EGFR phosphorylation; cyclosporine H, the FPR inhibitor inhibits EGF or NGF associated ROS production, EGF associated CD11b upregulation and prevents EGFR and TrkA phosphorylation by their respective ligand EGF and NGF."
reach
"We were unable to detect any significant differences between primary and immortalized cells in signal pathways such as epidermal growth factor stimulated epidermal growth factor receptor phosphorylation, insulin stimulated Akt phosphorylation, oxytocin and lysophosphatidic acid stimulated extracellular signal regulated kinase 1 and 2 phosphorylation, myosin light chain phosphorylation, and interleukin-1 induction of IkappaBalpha degradation."
sparser
"We were unable to detect any significant differences between primary and immortalized cells in signal pathways such as epidermal growth factor-stimulated epidermal growth factor receptor phosphorylation, insulin-stimulated Akt phosphorylation, oxytocin and lysophosphatidic acid-stimulated extracellular signal-regulated kinase 1 and 2 phosphorylation, myosin light chain phosphorylation, and interleukin-1 induction of IkappaBalpha degradation."
reach
"Our previous work indicated that GH, PRL, and EGF induce EGFR serine/threonine phosphorylation that requires ERK activity and is detected by PTP101, a monoclonal antibody that reacts with phosphorylated serine or threonine residues that reside within ERK substrate consensus sites [XREF_BIBR - XREF_BIBR]."
sparser
"The ability of CCK/gastrin analogs to cause EGFR transactivation was investigated. xref shows that addition of 0.1 µM CCK-8, CCK-8NS, gastrin-17 or EGF strongly, whereas CCK-4 moderately increased EGFR tyrosine phosphorylation 2 min after addition to NCI-H727 cells. xref shows that CCK-8, CCK-8NS, gastrin-17 and EGF increased significantly EGFR tyrosine phosphorylation by 233, 149, 208 and 250%, respectively."
sparser
"EGF induced cell migration in a dose-dependent manner; EGF-induced EGFR phosphorylation and downstream activation of c-Jun N-terminal protein kinase (JNK), p38 MAP kinase (p38), extracellular signal-regulated kinase (ERK1/2) and AKT, were inhibited by PD153035 (EGFR inhibitor), JNKi (JNK inhibitor), SB203580 (p38 inhibitor), U0126 (MEK/ERK inhibitor), and LY294002 (PI3K/AKT inhibitor), respectively."
reach
"Concentrating on two members of EGFR ligand family, i.e. EGF and AR, we show that AR and EGF induced similar patterns of short term EGFR and c-Cbl phosphorylation, physical association of c-Cbl with EGFR, and EGFR ubiquitination; however, as previously reported for TGF-alpha, the effects of AR were much more transient than those of EGF."
sparser
"In contrast, blockade or silencing of Src only suppressed EGF-induced EGFR phosphorylation at Y845 but did not affect EGFR autophosphorylation at Y1068 by EGF, indicating that ligand-dependent EGFR phosphorylation at Y845 depends on Src whereas autophosphorylation at other residues such as Y1068 is Src-independent."
reach
"Considering that EGF at 25ng/ml might maximally induced EGFR phosphorylation, so that no further increase occurred by LPC, the effect of LPC on EGFR phosphorylation induced by EGF at 5 and 10ng/ml was also examined; LPC did not affect EGF induced EGFR phosphorylation (data not shown)."
sparser
"Western blot analysis was used to determine the bioactivity of AG1478 released from encapsulated versus non-encapsulated AG1478 on inhibition of EGF-induced EGFR tyrosine phosphorylation in FR3T3 and A431 cells. ***since reviewers struggled, you might include a note here noting the mechanism and how phosphorylation plays into it)*** FR3T3 cells were treated with non-encapsulated AG1478 or supernatants from encapsulated AG1478 at the IC 50 value (6.0 nM, 1.9 ng mL −1 ) for 30 min and then challenged with EGF."
sparser
"However, we found that sulfasalazine had no effect on the EGF-induced EGFR phosphorylation of EGFR or its downstream targets ERK (extracellular signal–regulated kinase) and AKT ( xref ), suggesting that sulfasalazine reduces the viability of EGFR high /xCT high glioma cells without affecting EGFR signaling."
sparser
"Five biological effects were considered: inhibition of high affinity 125I-EGF binding, change in the phosphorylation state of the EGF receptor, inhibition of the EGF-dependent tyrosine phosphorylation of the EGF receptor, inhibition of [3H]phorbol 12 beta, 13 alpha-dibutyrate binding, and stimulation of calcium- and phospholipid-dependent protein kinase (C-kinase) in vitro."
reach
"Western blotting revealed that epidermal growth factor induced tyrosine phosphorylation of epidermal growth factor receptor was inhibited by RC-160, which suggests that the direct inhibitory effect of RC-160 on HEC-1 cell growth might be mediated in part by interference with epidermal growth factor receptor phosphorylation."
sparser
"Interestingly, after MβCD treatment, we observe a clear defect in the increase of EGF-induced tyrosine phosphorylation of EGFR at 1173 residue (pEGFR Y1173 ) but not at 1068 residue (pEGFR Y1068 ) (Fig. xref ), thus suggesting the presence of potential different roles between EGFR phosphorylation pattern and function of different tyrosine phosphorylation sites xref ."
sparser
"After 30 min of incubation, the compound significantly decreased phospho form of EGFR which further decreased with time upto 3 h in both MDA-MB 231 cells(p<0.05 to p<0.001) and primary breast adenocarcinoma cells (p<0.01 to p<0.001) while 100 ng/ml of EGF induced tyrosine phosphorylation of EGFR (p<0.01)."
reach
"Although the internalization of EGF receptor (EGFR) which is considered as the main mitogen receptor is completely blocked at 4degreesC XREF_BIBR, XREF_BIBR, the EGF stimulation induced phosphorylation and activation of EGFR and its effectors as well as their interaction are preserved and even enhanced XREF_BIBR - XREF_BIBR."
sparser
"The result demonstrated that EGF treatment (100 ng/ml, 5 min) led to significant increase of Tyr1068 and Tyr1173 phosphorylation of EGFR, and pre-treatment with CHAG (1000 μg/ml, 1 h) efficiently hindered the EGF-induced phosphorylation of EGFR (Figure xref ), indicating that CHAG inhibited EGF-induced activation of EGFR."
sparser
"We showed that gefitinib suppressed EGF-induced EGFR phosphorylation to basal levels at three phosphorylation sites studied, and it inhibited the activation-specific phosphorylation of the downstream signal pathway components AKT, ERK, STAT3, and NF-κB to various degrees in different HNSCC cell lines and tumors."
reach
"Our previous results showed that PKG II could inhibit EGF induced tyrosine 1068 phosphorylation of EGFR in gastric cancer cell line BGC-823 XREF_BIBR, raising the question whether PKG II can inhibit the phosphorylation of other tyrosine sites on EGF and EGFR and thereafter have a wide-range inhibition on EGF and EGFR induced signal transductions and related biological activities of gastric cancer cells."
sparser
"MCF-7 cells exhibited a mean 2.3-fold increase (95% confidence interval: 1.91, 2.65; P < 0.0001) in levels of epidermal growth factor phosphorylation in response to exposures of 2 Gy, which was substantially less than the epidermal growth factor receptor Y phosphorylation induced by epidermal growth factor."
reach
"In HT-H cells (human embryonal carcinoma that spontaneously differentiate into trophoblasts), induction of HER1 phosphorylation by EGF or HBEGF requires derepression of the receptor by trophinin, a transmembrane protein expressed in both human trophoblasts and the uterine epithelium that is capable of mediating cell adhesion through homophilic binding."
reach
"Densitometric analysis of the individual bands relative to beta-actin and total EGFR show that BPD-PDT treated samples were more responsive to EGF stimulation of EGFR activity than control cells; for BPD-PDT treated samples, the addition of EGF stimulated phosphorylation of EGFR to 180% of that observed in the no treatment control (XREF_FIG)."
sparser
"CRM197 (a non-toxic mutant of diphtheria toxin that specifically inhibits the mitogenic effects of cells producing HB-EGF) was able to block EGFR transactivation by GPCR agonists in COS-7 and HEK 293 cells; however, CRM197 did not affect exogenous EGF-induced EGFR tyrosine phosphorylation."
sparser
"Tyrphostin AG1478, the EGFR inhibitor, inhibits fMLP and NGF-associated ROS production, fMLP-associated CD11b upregulation and NGF-induced TrkA phosphorylation; K252a, the NGF receptor inhibitor, inhibits fMLP or EGF-associated ROS production, CD11b expression and EGF-induced EGFR phosphorylation; cyclosporine H, the FPR inhibitor inhibits EGF or NGF-associated ROS production, EGF-associated CD11b upregulation and prevents EGFR and TrkA phosphorylation by their respective ligand EGF and NGF."
reach
"We have previously reported that CXCR7 (independent of binding its ligand, stromal cell derived factor 1alpha [SDF-1alpha]) interacts with the epidermal growth factor receptor (EGFR), leading to increased EGF stimulated EGFR phosphorylation (particularly at tyrosine 1110 [Y1110]), enhanced downstream mitogenic signaling as well as tumor cell proliferation and survival XREF_BIBR, XREF_BIBR."
sparser
"Notably, however, no increased phosphorylation at any of these sites was detected with combination GH plus EGF treatment, suggesting that the source of the GH/EGF synergy in ERK activation was not the result of GH augmentation of EGF-induced EGFR tyrosine phosphorylation or activation."
sparser
"Moreover, four independent results indicate that the inhibition of EGF-stimulated Akt phosphorylation by clathrin perturbation was not the result of alterations in cell-surface EGFR levels, as CHC silencing was without effect on 1) cell-surface EGFR levels measured by antibody binding to intact cells ( xref ), 2) cell-surface EGFR levels measured by EGF binding to intact cells (Supplemental Figure S1E), 3) EGF-stimulated EGFR phosphorylation ( xref and Supplemental Figure S4A), and 4) EGF-simulated p42/44 MAPK phosphorylation (Supplemental Figure S4B)."
sparser
"Further research revealed that SIP-SII decreased the EGF-induced phosphorylation of epidermal growth factor receptor (EGFR), Akt and p38, but no significant suppression on EGF-induced phosphorylation of extracellular signal-regulated kinase 1 and 2 (Erk1/2) and c-Jun N-terminal kinases (JNK) by SIP-SII treatment was observed."
reach
"The induced DNA binding activity of AP-1 in young cells was inhibited by PD 98059, an inhibitor of MEK, and partially inhibited by GF 109203X, an inhibitor of protein kinase C. Western blot analysis demonstrated that EGF induced dramatic increase in the phosphorylation of EGF receptor (EGFR) and extracellular signal regulated kinases (ERK) in young cells, while this phosphorylation was much less profound in senescent cells."
sparser
"Our data showed that EGF (20 ng/mL for 30 min) strongly stimulated the phosphorylation of EGFR protein in 5 NSCLC cells while WB-308 reduced EGF-induced EGFR phosphorylation in a dose-dependent manner in these NSCLC cell lines (Figure xref for A549, PC-9, NCI-H1975 and S1A for NCI-H1650 and HCC-827)."
reach
"MCF-7 cells exhibited a mean 2.3-fold increase (95% confidence interval : 1.91, 2.65; P < 0.0001) in levels of epidermal growth factor phosphorylation in response to exposures of 2 Gy, which was substantially less than the epidermal growth factor receptor Y phosphorylation induced by epidermal growth factor."
reach
"So far we demonstrated that : an inverse correlation does exist between EGFR and ZNF216 molecules, both at mRNA and protein levels; EGF increased the expression of ZNF216 both in the cytosolic and in the nuclear fraction in a time dependent manner; in cells overexpressing ZNF216 protein, EGF increased EGFR phosphorylation in both the cytosolic and the nuclear fraction compared to control cells; a sustained phosphorylation of MAPK and Akt was observed after EGF treatment in ZNF216 overexpressing cells."
sparser
"Ligand-induced multiple phosphorylations
of receptor tyrosine kinases are widely believed to occur, based on
the results of ensemble biochemical assays; xref − xref however, our
recently published results call into question the prevalent model
that EGF induces multiple phosphorylations of EGFR on the cell membrane. xref Remarkably, we observed that the combinatorial
PTM distribution of rEGFR molecules was strongly biased toward monophosphorylation
codes (NT: 94.4%, EGF: 94.5%, xref d)."
sparser
"However, because the EGFR is functional, as shown by detection of activated EGF-induced EGFR phosphorylation, ERK1/2, p38 MAPK and PI3K/Akt, it is unclear whether the lack of an effect of EGF on E-cadherin expression in MPSC1 cells is due to the lack of H 2 O 2 production after EGF treatment."
sparser
"Inhibition of ADAM10/17 using TAPI-2 (10 μM; 1 h) significantly reduced UTP-induced EGFR phosphorylation, but had no effect on EGF-induced EGFR phosphorylation ( xref ), suggesting that metalloprotease-mediated shedding of EGFR ligands contributes to P2Y 2 R-mediated EGFR phosphorylation."
sparser
"EGFR isolated by wheat germ agglutinin affinity chromatography from nontreated cells exhibited substantial ligand-induced phosphorylation, and EGFR isolated from prolactin-treated cells displayed minimal EGF-induced EGFR phosphorylation, as well as decreased kinase activity toward exogenous substrates."
sparser
"In HT-H cells (human embryonal carcinoma that spontaneously differentiate into trophoblasts), induction of HER1 phosphorylation by EGF or HBEGF requires derepression of the receptor by trophinin ( xref ), a transmembrane protein expressed in both human trophoblasts and the uterine epithelium that is capable of mediating cell adhesion through homophilic binding ( xref )."
reach
"Together with the previous result, this result indicates that though HIP1R mediates the endocytosis of EGFR, it does not affect the EGF-stimulated EGFR phosphorylation which is prior to the endocytosis.Next, we examined EGFR-activated downstream signal transduction pathways involved in neurite outgrowth."
sparser
"Although the internalization of EGF receptor (EGFR) which is considered as the main mitogen receptor is completely blocked at 4°C xref , xref , the EGF stimulation-induced phosphorylation and activation of EGFR and its effectors as well as their interaction are preserved and even enhanced xref – xref ."
sparser
"Based on the findings that specific knockdown of CHKA attenuated the EGF-induced EGFR phosphorylation and no such effect was observed with siR/CTOR treatment, we conclude that the interaction of CHKA and EGFR is a prerequisite for the formation of EGFR/CHKA/RICTOR complex and EGFR/CHKA-induced mTORC2 activity."
sparser
"As shown in xref , although both Berberine and Costunolide reduced EGFR phosphorylation in PLC/PFR/5 cells cultured in full serum ( xref , 1 st panel from top, lane 3 and 4 compared to lane 2), but neither Berberine nor Costunolide blocked EGFR phosphorylation stimulated by EGF ( xref , 1 st panel from top, lane 8 and 9 compared to lane 7)."
sparser
"The data in xref – xref indicate that endogenous (wild-type) EGFR present along with endogenous ErbB-2 in fibrosarcoma cells undergoes EGF-induced PTP-101-reactive phosphorylation and that in that setting pharmacologic prevention of ERK activity augments EGF-induced EGFR downregulation and EGF-induced EGFR tyrosine phosphorylation."
reach
"The findings that depleting CaSR inhibited EGF stimulated phosphorylation of EGFR and ERK (XREF_FIG) while EGF inhibitor blocked Ca 2+ -induced activation of ERK (XREF_SUPPLEMENTARY) support a signaling scheme in which the E-cadherin and EGFR interface couples the CaSR to MAPK activation."
sparser
"An examination of the effect of sialylation on epidermal growth factor receptor (EGFR) activity and downstream signaling, which are highly correlated with cell proliferation, showed that the loss of ST6Gal-I augmented EGF-induced EGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) in colon cancer cells."
sparser
"Interestingly, EGF-induced tyrosine phosphorylation of the EGF receptor was strongly attenuated by 1O2 but unimpaired by C2-ceramide, implying that, although ceramide formation may mediate the above attenuation of ERK and Akt phosphorylation induced by 1O2, mechanisms beyond ceramide formation exist that mediate impairment of growth factor signaling by singlet oxygen."
sparser
"Consistent with in vitro studies with other cell lines( xref ), concentrations in the 1 uM micromolar range were sufficient in vitro to block EGF-induced phosphorylation of ERBB1 and ERBB2 ( xref ), lamellipod extension, chemotaxis and invasion ( xref ), while inhibition of proliferation required higher concentrations ( xref )."
reach
"Two types of modified GM3 strongly alter EGF dependent phosphorylation of the EGF receptor in opposite directions, i.e., de-N-acetyl-GM3 (amino-GM3; NeuNH2 alpha 2 ---- 3Gal beta 1 ---- 4Glc beta 1 ---- 1 Ceramide) strongly promotes tyrosine phosphorylation of the EGF receptor of A431 cells, while lyso-GM3 (NeuNAc alpha 2 ---- 3Gal beta 1 ---- 4 Glc beta ---- 1 Sphingosine) as well as GM3 inhibit tyrosine phosphorylation of the EGF receptor in the same cells under the same conditions."
sparser
"In addition, EGF induced the sustained tyrosine phosphorylation of the EGF receptor, mitogen-activated protein (MAP) kinases, and 46 and 52 kDa proteins, and the prolonged activation of MAP kinases in PC12h-R cells compared with the parent PC12h, which does not show EGF-induced differentiation."
sparser
"The upregulation of ANXA1 detected due to the peptide treatments could potentially cause the inhibition ( xref ), since ANXA1 negatively correlates with the activation of EGFR signaling via the EGF stimulated EGFR tyrosin 1068 phosphorylation and its downstream AKT activity ( xref )."
reach
"beta1AR stimulation and EGF ligand can each induce equivalent EGFR phosphorylation, internalization, and downstream activation of ERK1/2, but only EGF ligand causes translocation of activated ERK to the nucleus, whereas beta1AR-stimulated and EGFR-transactivated ERK is restricted to the cytoplasm."
reach
"Another study showed that AIB1 knockdown reduced EGF mediated phosphorylation of ErbB1 and ErbB2, and proposed a portion of the oncogenic effect of AIB1 could be through regulation of ErbB1 and ErbB2 activity and subsequent modulation of downstream cellular signaling pathways [XREF_BIBR]."
sparser
"In the current study, gefitinib treatment in our panel of STS cell lines with wild-type EGFR TK , KRAS and BRAF genes (so as to rule out any interference from relevant gene mutation) did not inhibit proliferation despite complete inactivation of EGF-induced EGFR phosphorylation, as well as inhibition of representative signal transducers (pAKT and pERK) of two main downstream pathways - RAS/RAF/ERK MAPK and PI3K/AKT."
sparser
"In this study, it was observed that in cells conditioned for differentiation SCD5 blunted the EGF-induced phosphorylation and activation of EGFR, as well as its downstream signals ERK and Akt, which are central players in neuronal replication and functional maturation [ xref , xref ]."
sparser
"Oxidative stress responses initiated following PDT can lead to the degradation of the EGFR immediately following PDT [ xref ; xref ]; however, in the present study we demonstrate that PDT sensitizes EGFR expressing OVCAR-5 cells to EGF-induced phosphorylation of EGFR and downstream signaling 24 hours following PDT ( xref )."
sparser
"FSH itself does not affect epidermal growth factor receptor (EGFR) concentration or the tyrosine phosphorylation of EGFR, but it enhances the EGF-induced tyrosine phosphorylation of EGFR, indicating that the FSH signaling pathways may stimulate or modulate specific EGFR–regulating proteins."
sparser
"Our previous work indicated that GH, PRL, and EGF induce EGFR serine/threonine phosphorylation that requires ERK activity and is detected by PTP101, a monoclonal antibody that reacts with phosphorylated serine or threonine residues that reside within ERK substrate consensus sites [ xref – xref ]."
sparser
"Previous studies indicate that CP724714 is a potent inhibitor of HER2 auto-phosphorylation with no detectable effect on EGF-induced HER1 phosphorylation. xref As shown in xref (upper panel), incubation with 50 μM CP724714 effectively abolished IR-induced HER2 phosphorylation in MCF-7 cells."
reach
"Like the mouse 10T (1/2) fibroblasts, breast tumor cell lines that co-overexpress c-Src and HER1 display EGF induced complex formation between c-Src and HER1, the appearance of Tyr 845 and Tyr 1101 phosphorylations on the c-Src-associated receptor, and increased phosphorylation of the HER1 effectors Shc and mitogen activated protein kinase (MAPK), as well as increased tumor size in nude mice when compared with the majority of cell lines that do not overexpress these tyrosine kinases or express only one of the pair."
sparser
"Previous studies reported that silencing of GALNT2 could inhibit insulin-induced insulin receptor activation and Akt phosphorylation in hepatoma cells [ xref ], whereas overexpression of GALNT2 in oral cancer cells could promote EGF-induced phosphorylation of EGFR and Akt [ xref ]."
sparser
"As expected, H292-shDUOX1 cells were resistant to ATP-induced EGFR phosphorylation at Y845 and Y1068 compared to corresponding control cells (H292-shCTL), although EGF-dependent EGFR phosphorylation was largely unaffected, and were also resistant to ATP- or EGF-dependent activation of Src, STAT3 or ADAM17 ( xref )."
sparser
"Whereas the EGF-induced phosphorylation of the EGFR and the adapter protein insulin-receptor substrate 1 (IRS1) was drastically reduced by gefitinib ( xref ) the phosphorylation of Akt was not ( xref ), indicating that Akt signaling was unchanged in gefitinib-treated H460 cells."
sparser
"Investigation of the biological effects of the interaction revealed that activation of EGFR induces the phosphorylation of IQGAP1 at Ser 1443 through protein kinase C α (PKCα). [Silencing PKCα with siRNA and pharmacological inhibition of PKC abrogate EGF-induced IQGAP1 Ser 1443 phosphorylation.] Moreover, IQGAP1 is required for EGF to induce phosphorylation of EGFR as tyrosine phosphorylated EGFR is not detectable after EGF stimulation of IQGAP1-deficient mouse embryonic fibroblast (MEF) cells."
sparser
"Our studies of the mechanism underlying the functional differences between ErbB4 full and partial agonists have been inspired by the observation that the EGFR full agonist AR stimulates a pattern of EGFR tyrosine phosphorylation that is distinct from the pattern of EGFR tyrosine phosphorylation stimulated by the EGFR partial agonist EGF [ xref ]."
reach
"Treatment with integrin alpha v beta 3 function blocking antibody inhibits EGF stimulated EGFR phosphorylation and cell proliferation [XREF_BIBR] Integrin alpha v beta 3 has been reported to interact with PDGFRbeta and modulates the PDGFRbeta function in fibroblasts [XREF_BIBR] and endothelial cells [XREF_BIBR]."
sparser
"Considering that EGF at 25 ng/ml might maximally induced EGFR phosphorylation, so that no further increase occurred by LPC, the effect of LPC on EGFR phosphorylation induced by EGF at 5 and 10 ng/ml was also examined; LPC did not affect EGF-induced EGFR phosphorylation (data not shown)."
reach
"The induced DNA binding activity of AP-1 in young cells was inhibited by PD 98059, an inhibitor of MEK, and partially inhibited by GF 109203X, an inhibitor of protein kinase C. Western blot analysis demonstrated that EGF induced dramatic increase in the phosphorylation of EGF receptor (EGFR) and extracellular signal regulated kinases (ERK) in young cells, while this phosphorylation was much less profound in senescent cells."
sparser
"As it is known that transactivation of Met can occur via epidermal growth factor (EGF)-dependent EGF receptor (EGFR) activation or through a constitutively activated EGFR pathway xref , we examined whether inhibition of EGF-induced phosphorylation of EGFR by the dimeric macrocycles caused the ‘bell-shaped’ response mentioned above."
sparser
"In addition, in D283 cells, a cell line derived from medulloblastoma metastasis in the peritoneum, ouabain did not inhibit EGF-induced activation of Erk1/2 but reduced EGF-induced Akt activation (Fig. S2A) and almost completely abolished EGF-induced EGFR phosphorylation (Fig. S2B)."
sparser
"Although our study did not investigate the precise nature of the METex14-ERBB interaction, previous studies have shown that MET and ERBB family members can be co-immunoprecipitated ( xref ), EGF-stimulated EGFR phosphorylation leads to transactivation of MET ( xref , xref ); MET and ERBB2 cooperate to enhance cell invasion ( xref ); and MET phosphorylation leads to activation of EGFR or ERBB3 ( xref , xref )."
sparser
"Although there was a high level of EGFR phosphorylation in A431 cells pretreated with PEG-dexSPIONs, we observed a dose-dependent inhibition of epidermal growth factor-induced EGFR phosphorylation in response to pretreatment of A431 cells with cet-PEG-dexSPIONs, with an almost complete inhibition of phosphorylation at an iron concentration of 50 μg/mL cet-PEG-dexSPIONs."
reach
"Ras activation by NEU3 was abrogated by PP2 (src inhibitor) or AG1478 (epidermal growth factor receptor (EGFR) inhibitor), and NEU3 actually enhanced EGF stimulated tyrosine phosphorylation of EGFR, suggesting that the upstream targets might be tyrosine kinases including src and EGFR, and the subsequent stimulation of Ras cascade leads to the inhibition of cell apoptosis."
sparser
"EGF‐induced phosphorylation of EGFR and activation of the Raf‐MEK‐ERK cascade was negatively affected by the silencing of filamin A both in vitro and in vivo. xref Rajesh K Nallapalli xref suggested that in addition to its classically known cytoskeletal function, filamin A also plays an important role in the activation of ERK and AKT signaling pathways during K‐RAS‐induced transformation."
sparser
"RB200 inhibited EGF- and NRG1-beta1-stimulated tyrosine phosphorylation of HER family proteins, proliferation of a diverse range of tumor cells in monolayer cell growth assays, tumor cell proliferation as a single agent and in synergy with tyrosine kinase inhibitors, lysophosphatidic acid-stimulated cell proliferation, and tumor growth in two human tumor xenograft nude mouse models."
sparser
"EGF induced significant phosphorylation of EGFR and ERK, and slight phosphorylation of P38MAPK, JNK, MAPKAPK2, HSP27, and c-Jun. By contrast, treatment with 1.0 μM AF for 12 hours caused slight phosphorylation of EGFR and ERK, but significant phosphorylation of P38MAPK, JNK, MAPKAPK2, c-Jun, and HSP27."
reach
"Our data indicates that EGF stimulation can activate the well-known phosphorylation of EGFR and downstream signaling molecules, such as mitogen activated protein kinases (MAPK1 and MAPK3), however, it also leads to substantial modulation of reversible cysteine modifications in numerous proteins."
sparser
"Western blotting revealed that epidermal growth factor-induced tyrosine phosphorylation of epidermal growth factor receptor was inhibited by RC-160, which suggests that the direct inhibitory effect of RC-160 on HEC-1 cell growth might be mediated in part by interference with epidermal growth factor receptor phosphorylation."
sparser
"Interestingly, knockdown of TRP14 in HEK293 cells further increases EGF-dependent EGFR phosphorylation that is accentuated by exogenous polysulfide addition, suggesting a regulatory function of per- or polysulfidation of PTP1B counteracted by TRP14 during EGF signaling [ xref ]."