IndraLab

Statements

UCHL5 binds NLRP3. 23 / 23
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"Together, these data verify that UCHL5 directly interacts with NLRP3 and induces the activation of NLRP3 inflammasomes."
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sparser
"Furthermore, we indicated that UCHL5 directly interacts with NLRP3 and induces the activation of NLRP3 inflammasomes in M-MSCs, which inhibits OB differentiation by promoting NLRP3 de-ubiquitination (Figure xref )."
36778113
sparser
"However, UCHL5 did not interact with NLRP3 or with EST12 in RACK1-deficient macrophages upon EST12 stimulation (fig."
33097533
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"S3D), suggesting that RACK1 could serve as a scaffold protein or a bridge between EST12 and UCHL5-NLRP3."
33097533
sparser
"However, whether UCHL5 interacts with NLRP3 in M-MSCs remains unclear."
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reach
"Mechanistically, NLRP3 directly bound to UCHL5 and maintained its stability through reducing ubiquitin-proteasome pathway degradation in mandibular MSCs."
36778113
sparser
"In our study, we further confirmed that UCHL5 interacts directly with NLRP3 and predicted the associated binding sites at the molecular level."
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reach
"Mechanistically, NLRP3 directly binds to UCHL5 and maintains its stability through reducing ubiquitin-proteasome pathway degradation in M-MSCs."
36778113
reach
"To deduce insight into the nature of molecular interactions between NLRP3 and UCHL5, we performed protein-protein docking studies."
36778113
reach
"Through analysis, we observed that UCHL5 could directly bind to NLRP3, and vice versa."
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reach
"We next performed immunofluorescence assays to further investigate the binding of UCHL5 to NLRP3 in M-MSCs from WT and NLRP3 mice treated with or without LPS ± ATP."
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sparser
"Nevertheless, the interaction between UCHL5 and NLRP3 in AS remains unclear."
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reach
"Furthermore, through co-immunoprecipitation (IP) analysis, we observed that both NLRP3 and RACK1 directly interacted with UCHL5 after EST12 stimulation (XREF_FIG), and EST12-Y80A stimulation could not promote the interaction of RACK1 with UCHL5 and NLRP3 (XREF_FIG)."
33097533
reach
"Nevertheless, the interaction between UCHL5 and NLRP3 in AS remains unclear."
37044315
sparser
"Mechanistically, NLRP3 directly bound to UCHL5 and maintained its stability through reducing ubiquitin-proteasome pathway degradation in mandibular MSCs."
36778113
sparser
"Mechanistically, NLRP3 directly binds to UCHL5 and maintains its stability through reducing ubiquitin-proteasome pathway degradation in M-MSCs."
36778113
sparser
"We next performed immunofluorescence assays to further investigate the binding of UCHL5 to NLRP3 in M-MSCs from WT and NLRP3 KO mice treated with or without LPS ± ATP."
36778113
sparser
"UCHL5 interacts with NLRP3."
36778113
sparser
"To deduce insight into the nature of molecular interactions between NLRP3 and UCHL5, we performed protein-protein docking studies."
36778113
sparser
"For further validation, we determined whether UCHL5 interacts with NLRP3."
36778113
sparser
"Through analysis, we observed that UCHL5 could directly bind to NLRP3, and vice versa."
36778113
sparser
"Furthermore, LPS stimulation could promote the interaction of UCHL5 with NLRP3 (Figure xref C)."
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biogrid
No evidence text available
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