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VHL binds OTUD6B. 17 / 17
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"OTUD6B interacted with pVHL tumor-derived missense mutants and reduced their ubiquitylation in cells."
35110537
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"Further we reveal that OTUD6B interacts with pVHL and reduces the ubiquitylation of pVHL in an enzyme‐independent manner."
32328410
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"The results showed that ectopic OTUD6B interacted with indicated pVHL mutants in HEK293 cells, but compared with pVHL wild type and the majority of mutants, the binding between pVHL I151T mutant with OTUD6B was markedly weakened (Fig. xref )."
35110537
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"Given that OTUD6B is integrated into the Cullin 2-Elongin B/C complex, it’s interesting to speculate whether it can form additional E3 complex besides pVHL to play a more general role together with Cullin 2-Elongin B/C. In addition, why does pVHL only bind with OTUD6B but not other OTU family members, such as OTUD6A? To this end, further structural study regarding the difference between the N-terminal domain (NTD) of OTUD6B and OTUD6A could provide more insights."
32323143
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"In addition, OTUD6B depletion reduced the inhibitory effects of ectopic pVHL missense mutants on cell migration and HIF-2α level, except for pVHL I151T. Thus, we speculated that I151 residue might be one of key sites of pVHL binding to OTUD6B. These results suggested that OTUD6B is an important regulator for the stability of pVHL missense mutants, which provides a potential therapeutic strategy for ccRCC with VHL mutations."
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"OTUD6B directly interacts with pVHL, decreases its ubiquitylation and proteasomal degradation to reduce HIF-1alpha accumulation in HCC cells under hypoxia."
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"OTUD6B directly interacts with pVHL, decreases its ubiquitylation and proteasomal degradation to reduce HIF‐1α accumulation in HCC cells under hypoxia."
32328410
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"Here, we tested the interaction between OTUD6B with pVHL mutants."
35110537
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"Subsequently, we examined the interaction between pVHL and OTUD6B. Endogenous OTUD6B and pVHL were coimmunoprecipitated from lysates of MHCC‐LM3 cells ( Figure xref )."
32328410
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"Indeed, further biochemical studies showed that, OTUD6B binds directly with pVHL rather than HIF-1α."
32323143
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"In addition, why does pVHL only bind with OTUD6B but not other OTU family members, such as OTUD6A?"
32323143
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"In addition, OTUD6B depletion reduced the inhibitory effects of ectopic pVHL missense mutants on cell migration and HIF-2α level, except for pVHL I151T. We speculated that I151 residue might be one of key sites of pVHL binding to OTUD6B. Altogether, these results suggested that OTUD6B is an important regulator for pVHL missense mutants, which provides a potential therapeutic strategy for ccRCC with VHL mutations."
35110537
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"Ectopic OTUD6B interacted with pVHL in HEK293 cells (Figure xref ; Figure S4B, Supporting Information)."
32328410
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"OTUD6B Interacts with pVHL."
32328410
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"At the same time, we observed that OTUD6B depletion reduced the inhibitory effects of pVHL wild-type and the mutants on cell migration and HIF-2α level, except for pVHL 151T mutant (Fig. xref a and b), suggesting that I151 residue might be one of key sites of pVHL binding to OTUD6B."
35110537
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"Considering that I151T mutation maps to the β domain, the OTUD6B-binding region of pVHL [ xref ], we proposed that I151 might be one of key sites of pVHL binding to OTUD6B."
35110537
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"Thus, we speculated that I151 residue might be one of key sites of pVHL binding to OTUD6B."
35110537