IndraLab

Statements

TGFB1 increases the amount of SMAD2. 57 / 69
| 54 2 1
reach
"TGFbeta1 increased the mRNA level and protein expression of Smad2, 3, 7 in HSC; it also increased protein expression of collagen I and III."
16313727
reach
"Previous in vitro studies have identified that TGF-beta1 increased expression of p-Smad2 and collagen content, and converted rat atrial fibroblasts into myofibroblasts, which induced the atrial fibrosis phenotype."
30894138
reach
"The levels of p-Smad2 (XREF_FIG) and TbetaRII (XREF_FIG) increased by SW620-S or exogenous TGF-beta1 were decreased to the normal level by SB431542, which indicated that this up-regulation was through the TbetaRII-ALK5-Smad2/3 pathway."
24427302
reach
"RLX has been found to inhibit TGF-beta1 signal transduction at the level of intracellular Smad2, either through direct effects on cyclic guanosine monophosphate (cGMP) or by signaling through a RXFP1-extracellular signal regulated kinases (ERK)-1/2 phosphorylation-neuronal nitric oxide (NO) synthase (nNOS)-NO-soluble guanylyl cyclase (sGC)-cGMP-dependent pathway."
32848798
reach
"In addition to p38 activation, TGF-beta1 treatment has been shown to upregulate the expression of p-smad2, which is traditionally believed to be an inducer of apoptosis and a tumor suppressor."
24959295
reach
"18 As shown in XREF_FIG, TGFbeta1 significantly increased the expression of SMAD2, a key factor of TGFbeta signaling pathways."
28067907
sparser
"AR12286 reduced the phosphorylated SMAD2 expression that is induced by TGF-β1."
32993110
reach
"A 54-day-long induction of TGF-beta1 in 3-month-old transgenic mice by withdrawal of doxycycline strongly increased the levels of pSmad2 in the DG."
24779367
reach
"In the Sui66 cell line (wild-type ACVR1B and SMAD4 genes), both TGFB1 and activin A increased the phosphorylation levels of SMAD2; these effects were cancelled by the ACVR1B/TGFBR1/ACVR1C specific inhibitor SB431542."
24886203
reach
"In the Sui68 cell line (homozygous deletion of ACVR1B gene and wild-type SMAD4 gene), TGFB1, but not activin A, increased the phosphorylation levels of SMAD2; these effects were cancelled by SB431542."
24886203
reach
"TGF-beta1 increased the invasive ability of JEG-3 cells and the expression levels of Smad2 and Smad3; hence, TGF-beta1 promotes the invasion of JEG-3 probably by activating Smad2 and Smad3."
28432277
reach
"The results of western blot suggested that TGF-beta1 increased the phosphorylation expression of Smad2."
27293383
reach
"As shown in XREF_FIG, the functional phosphorylation level of Smad 2 and Akt is up-regulated by TGF-beta1 treatment in human cardiac fibroblasts."
27098600
reach
"To investigate whether the TGFbeta1 induced TAGLN expression is mediated by the SMAD2/3 pathway, we tested phosphorylation levels of SMAD2 and found that TGFbeta1 treatment increased phospho-SMAD2 levels (XREF_SUPPLEMENTARY)."
27490926
reach
"In addition, TGF-beta1 markedly increased the expression levels of p-Smad2 in the A549 negative control cells and the A549-p130cas-RNAi cells following 1 h of treatment (XREF_FIG)."
24959295
reach
"In LX-2 cells, we observed that TGFbeta1 induced the expression of Smad2, 3, and 7, TbetaRI and TbetaRII, which was attenuated by ASTX."
25450180
reach
"The use of LY2109761, a selective inhibitor of TGF-beta1 and Smad2, diphenyliodonium (DPI), and/or astragaloside IV (AST IV is a monomer located in an extract of astragaloside) decreased the NOX4 expression, the level of ROS, and the TGF-beta1 and Smad2 expression, suggesting that inhibitions of the NOX4 and TGF-beta1 and Smad2 pathways downregulate apoptosis in HUVECs 30."
29371661
reach
"Addition of SB431542 also inhibited the expression of p-Smad2 produced by addition of TGF-beta1 (0.5 ng/ml)."
25681764
reach
"In order to rule out clonal artefacts, we transiently co-transfected PANC-1 cells with FLAG tagged Smad2 along with either HA tagged FRNK or MYC tagged dn Rac1 and evaluated levels of p-Smad2 following TGF-beta1 stimulation."
21624123
reach
"Our findings suggested that the expression of SOX9, COL10A1 and p-Smad2 was enhanced by TGF-beta1 stimulation in GC cells, further indicating that COL10A1 might play an important role in the TGF-beta1 and Smad2 downstream signaling pathway."
30154451
reach
"Our findings are partly in agreement with a report that TGF-beta1 increased Smad2 mRNA levels without affecting Smad3 mRNA expression [XREF_BIBR]."
28432277
reach
"TGF-beta1 significantly increased Smad 2 expression and decreased PPAR-gamma expression in NRK52E cells compared with the control group, while evodiamine and rosiglitazone almost reversed these effects."
24604887
reach
"TGF-beta1 increased alphaSMA expression and phosphorylation of SMAD-2."
21251657
reach
"In this study, we showed that TGF-beta1 promoted the synthesis and secretion of ET-1 in A549 cells, increased the expression of phospho-p38 MAPK and phospho-Smad2 and induced an increase in nuclear transfer of Smad2."
27293383
reach
"In the absence of TGFBR2 expression (-dox), TGF-beta1 treatment induced a basal level of pSMAD2 in both parental HCT116-Tet-on and HCT116-TGFBR2 cells."
23468914
reach
"Finally, to determine if TGFbeta signaling pathway was activated in cervical tissue we evaluated the expression of Smad2 and Smad3 which are known to be activated by TGFbeta1."
19631925
reach
"Inhibition of PPAR-gamma can suppress the expression of PPAR-gamma mRNA and increase the expression of ET-1 mRNA and protein mediated by TGF-beta1, increase the expression of P-P38 and P-Smad2."
27293383
reach
"TGF-beta1 can upregulate the expression of Smad2 and Smad3 during tissue fibrosis, a process that is negatively regulated by Smad7 as part of a negative feedback loop."
33365066
reach
"NOF-CAF cells ' expression of p-SMAD2 and p-SMAD3 in response to TGF-beta1 demonstrated intact TGF-beta signaling."
30087253
isi
"Then, we found that depletion of GPR37 resulted in a decrease in the expression of TGF-beta1 as well as the extents of Smad2 and Smad3 phosphorylation, while overexpression of GPR37 presented opposite outcomes."
33364431
reach
"Additionally, this study provided the first evidence that the NO donor, DEA/NO, could also induce anti-fibrotic actions via a sGC-cGMP-dependent pathway, leading to inhibition of TGF-beta1 signal transduction (at the level of pSmad2) and subsequently, the pro fibrotic influence of TGF-beta1 on renal myofibroblast differentiation, while being able to promote collagen degrading gelatinases (MMP-2 and MMP-9)."
27065874
reach
"As expected, the addition of exogenous TGF-beta1 increased the amount of pSMAD2 produced by BDC2.5 splenocytes, reaching its maximum level at 30-60 minutes after stimulation (data not shown)."
28170177
reach
"In Nesprin-2 KD cells TGFbeta1 stimulation led to a retarded nuclear accumulation and expression of SMAD2, 3 and 4 and c-Fos."
22198684
reach
"We firstly examined the expression of Smad2 and Smad3 induced by TGF-beta 1 in normal NIH/3T3 cells."
18506886
reach
"By contrast, AG490 treatment reversed the TGFbeta1 induced protein expression of pSmad2 and Twist (XREF_FIG)."
29115395
reach
"The administration of TGF-beta1 induced the expressions of miR-143-3p, p-Smad2, and collagens in cardiac fibroblasts (CFs) and the effect was inhibited by atorvastatin."
31758917
reach
"Along these lines, TGFbeta1 induced the protein expression of pSmad2 and Twist in accordance with the activated JAK and STAT3 signaling."
29115395
reach
"TGFbeta1 increased pSMAD2 levels, however the levels were not altered by TNFalpha pre-treatment (0/1 vs. 50/1 and 0/0 vs. 50/0; XREF_SUPPLEMENTARY)."
32714905
reach
"Western blot analysis revealed a high level of p-Smad2 and p-Smad3 in response to a 1-hour treatment with TGF-beta1."
31496148
reach
"As shown in Figure XREF_FIG, increased expression of both pSmad2 and LEFTY in response to TGF-beta1 was observed in OVISE and Ishikawa cells, whereas such effects were only minor in TOV-21G cells."
28969018
reach
"Consistent with these reports, we found that TGF-beta1 could up-regulate the protein expression of TGF-beta RI, TGF-beta RII, p-Smad2 and p-Smad3."
28458155
reach
"TGF-beta1 treatment increased the expression of pSmad2 in the nuclei of HMVECs, and the percentage of HMVECs positive for pSmad2 were significantly increased from 8.2 +/- 6.3% to 52.7 +/- 18.3% follow[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19608867
reach
"It was previously shown that RLX mediated its anti-fibrotic effects through direct or indirect activation of cGMP, the latter involving nNOS-NO-sGC-cGMP signaling, to inhibit TGF-beta1 signal transduction at the level of intracellular Smad2 and/or Smad3 in myofibroblasts."
32848798
reach
"In the present study, TGF-beta1 treatment was shown to increase the phosphorylation levels of Smad2 and Smad3, confirmed by the enhancement of the transcription and expression of collagen mRNA shown in XREF_FIG, XREF_FIG, XREF_FIG."
23418473
sparser
"While the expression level of Smad2 was not affected by miR-6315 treatment ( p > 0.05, NC vs. miR-6315, xref C), supplementation of TGF-β1 significantly induced Smad2 expression ( p < 0.0005, NC vs. NC+TGF-β1)."
| PMC
reach
"Furthermore, TGF-beta1 also up-regulated p-Smad2 levels, and down-regulated Smad7 levels in the cells (XREF_FIG)."
25464508
reach
"In hepatocyte specific TGFbetaR2 knockout mice with 70% hepatectomy, inhibition of TGFbeta1 and TGFbetaR2 promoted hepatocyte proliferation, increased the volume of the liver after surgery, and increased the expression of cyclin E and SMAD2 XREF_BIBR."
33754025
reach
"TGF-beta1 increased the expression of NFYA, TGFbetaR1, TGFbetaR2, p-Smad2, p-Smad3, p-Smad3L, and p-Akt (Ser473), and knocking down NFYA decreased the expression of these genes, except for TGFbetaR2."
28212548
reach
"Exogenous TGF-beta1 induced temporally dependent alterations in Smad2 and Smad3 gene expression."
19374154
reach
"However, the selective inhibitor of the TGF-beta1 and Smad signaling pathway, SB431542, significantly decreased the protein expression of TGF-beta1, p-Smad2 and Smad2, p-Smad3 and Smad3 and Smad7 in NFs."
33456506
reach
"TGF-beta1 could upregulate the expression of p-Smad2 in RAW264 cells, and this action was suppressed by the addition of a neutralizing antibody against TGF-beta or by SB431542."
25681764
reach
"The results revealed that TGFbeta1 induced the protein expression of pSmad2 and Twist (XREF_FIG)."
29115395
reach
"In the Western blot analysis it is also shown that TGF-beta1 alone is increasing the level of pSmad2 expression in absence of the TGFBR2 compared to the unstimulated cells (XREF_FIG)."
26114631
reach
"P-SMAD2 levels were partially rescued by TGF-beta1 treatment (0.46 +/-0.04; Bonferroni post hoc SB431542+ TGF-beta1 vs. SB431542 : p = 0.013; SB431542+ TGF-beta1 vs. vehicle : p = 0.033; XREF_FIG)."
26059637
reach
"We found that the mRNA levels of Smad2, Smad3, Smad4, and Smad7 were significantly increased by TGFbeta1."
25450180
reach
"siRNA mediated silencing of TGF-b1 in rats led to significantly reduced expression of Smad 2, 3 and 4, but significantly increased expression of Smad 7."
22964151
reach
"The protein expression levels of p-Smad2 and p-Smad3, which are enhanced by TGF-beta1, were markedly decreased in Rg3 treated KFs (XREF_FIG)."
29328420