IndraLab

Statements

SETDB1 methylates TP53. 9 / 9
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"Therefore, we decided to test whether SETDB1 could methylate p53."
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"While endogenous SETDB1 can methylate either mutant or wild-type p53, mutant p53 appears to be methylated to a higher degree (XREF_SUPPLEMENTARY)."
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"To ask whether SETDB1 can directly methylate full-length p53, we performed the in vitro methylation assay using the endogenous SETDB1 pull down complex and recombinant wild-type p53 protein."
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"Therefore, we decided to test whether SETDB1 could methylate p53."
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"In cancer cells that harbour the GOF, oncogenic TP53 mutations, such as R249S, the p53 protein is stabilized by interaction with and being methylated by SETDB1."
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"Since wild-type p53 is only present in cells at a very low level, methylation of the wild-type tumour-suppressive p53 by SETDB1 may contribute very little to cell growth."
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"We next tested methylation of endogenous p53 by endogenous SETDB1."
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"We next tested methylation of endogenous p53 by endogenous SETDB1."
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"Since wild-type p53 is only present in cells at a very low level, methylation of the wild-type tumour-suppressive p53 by SETDB1 may contribute very little to cell growth."
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