IndraLab

Statements

CALM1 binds M109Q. 6 / 6
| 6
sparser
"Direct in-cell measurements of RyR and CaMKII activity are not yet experimentally accessible, so right now we are not able to directly measure the impact of CALM1-M109Q on cellular RyR and CaMKII activation."
| PMC
sparser
"The AMPK nutrient-sensing pathway, which involves Ca2+ release and multiple CaM-dependent targets, negatively regulates myogenesis (Fulco et al., 2008; Williamson et al., 2009), so we hypothesized that inhibitors of this pathway might recover myogenesis in CALM1-M109Q myoblasts."
| PMC
sparser
"We added panels Fig. 2F and Fig. 2G that include investigations of whether the CALM1-M109Q mutation alters mitochondrial structure or lysosome distribution, and whether the myogenesis defect could be rescued by inhibiting the AMPK pathway, as described above."
| PMC
sparser
"We didn’t observe any impact due to CALM1-M109Q. In addition to Figure 2F, we added the following text to a new (second) paragraph of the results section entitled The calmodulin redox sensor halts myogenesis:"
| PMC
sparser
"The CRISPR-Cas9 method was used to generate a CAM1-M109Q mutant that mimicked the oxidative modification of one of 9 methonines in one or both alleles of the three expressed CaM genes in C2C12 mouse myoblast."
| PMC
sparser
"We added these results as Figure 2F that includes an investigation of whether the CALM1-M109Q mutation alters mitochondrial structure or lysosome distribution and dynamics, both of which are redox regulated cell processes involving CaM."
| PMC