IndraLab

Statements

IRF3 binds USP22. 11 / 11
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"USP22 interacted with IRF3 after viral infection in a KPNA2-dependent manner."
32130408
sparser
"USP22 is associated with IRF3 after viral infection."
32130408
sparser
"Knockdown of KPNA2 impaired virus-induced USP22-IRF3 association in MEFs ( xref ), indicating that USP22 interacts with IRF3 through KPNA2 after viral infection."
32130408
sparser
"Viral infection induced USP22-IRF3 association in the cytoplasm in a KPNA2-depedent manner, and knockdown or knockout of USP22 or KPNA2 impaired IRF3 nuclear translocation and expression of downstream genes after viral infection."
32130408
sparser
"In this context, we observed that IRF3(IL139/140AA), which is predominantly located in the nucleus, did not interact with USP22, and USP22(RR164/165AA) interacted with IRF3, as did wild-type USP22."
32130408
sparser
"We observed that USP22 interacted with IRF3 and phosphorylated IRF3 (pIRF3) in the cytoplasm but not in the nucleus after vesicular stomatitis virus (VSV) or HSV-1 infection in BMDCs ( xref )."
32130408
sparser
"Interestingly, we found that the USP22(RR164/165AA) was still associated with IRF3 ( xref )."
32130408
sparser
"These data suggest that USP22 interacts with IRF3 in the cytoplasm after viral infection."
32130408
sparser
"Because USP22-mediated virus-triggered signaling requires its enzyme activity and USP22 interacts with IRF3, we hypothesized that USP22 might catalyze deubiquitination of IRF3 and regulate the nuclear translocation of IRF3."
32130408
sparser
"Together with the observations that knockdown of KPNA2 impaired IRF3-USP22 associations after viral infection and that reconstitution of KPNA2 into USP22 knockout cells restored virus-induced nuclear translocation of IRF3 and expression of downstream genes, we concluded that USP22 promotes nuclear translocation of IRF3 primarily through deubiquitinating and stabilizing KPNA2."
32130408
sparser
"Such an intermediate protein should associate with both IRF3 and USP22 and promote virus-triggered signaling by facilitating the nuclear translocation of IRF3."
32130408