IndraLab

"Phospholipase C gamma 1 (PLC gamma 1) and p21ras guanosine triphosphatase (GTPase) activating protein (GAP) bind to and are phosphorylated by activated growth factor receptors"

"From Fig. 4: Using the same time and dose strategies as before, a mean 6.2-fold induction of PLC{gamma} phosphorylation was observed following radiation treatment (Fig. 4). Increases in phosphorylation of Shc family members were observed following irradiation; the mean -fold changes (n = 3) were 2.3, 3.0, and 3.5 for the 66-, 52-, and 46-kDa isoforms, respectively. For both PLC{gamma} and Shc, EGF at 10 ng/ml induced a significantly greater increase in phosphorylation than IR (p < 0.05). Pretreatment of cells with 500 nM AG1478, a specific inhibitor of EGFR Tyr phosphorylation (29), abolished the radiation- and EGF-induced activation of both PLC{gamma} and Shc in A431 cells."