IndraLab

Statements

CALM1 is phosphorylated. 56 / 56
| 35 21
rlimsp
"Binding of CaM to the cSH2 domain can help release the inhibition imposed on the p110 subunit, similar to the binding of the phosphorylated motif of RTK, or phosphorylated CaM (pCaM), to the SH2 domains."
29494137
sparser
"All reactions were monitored by thin layer chromatography (TLC) carried out on Merck silica gel plates (0.25 mm thick, 60F254), visualized by using UV (254 nm) or dyes such as ninhydrin, KMnO 4 , p -a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25882520
rlimsp
"A system of digitonin-permeabilized islet cells was developed to characterize Ca2+- and calmodulin-dependent protein phosphorylation further and to determine whether activation of this membrane-bound process was sufficient for initiation of Ca2+-stimulated insulin secretion."
3896230
sparser
"Interestingly, we observed that phosphorylation of CaM1 only has a modest impact on its affinity for calcium."
22870263
sparser
"The basal and agonist induced phosphorylation of CAM was carefully analyzed ( Thomas et al., 2000 )."
19061936
rlimsp
"Western blotting results indicated that berberine treatment decreased phosphorylation of calmodulin kinase II and blocked subsequent MEK1 activation as well as p27 protein degradation."
23421648
sparser
"An involvement of mTORC2 in calcium regulation in muscle is also supported by the decrease in the phosphorylation of calmodulin 1 (CALM1) in Ric mKO muscle, since calmodulins are calcium ion sensor proteins that transduce most of the calcium signalling to downstream protein kinases and phosphatases (Marcelo et al. 2016)."
28464351
sparser
"The analyses exhibited a sharp overexpression as well as a potential correlation of E p CAM to the LC development."
34345719
sparser
"Surprisingly, no increase of CAM basal phosphorylation was observed in comparison to the WT receptor."
19061936
sparser
"After salt-induced CAM induction, the six genes were expressed with more prominent fluctuations than before stress, suggesting that these PP2C genes may be involved in the diurnal regulation of protein phosphorylation in CAM."
32689386
rlimsp
"Selective phosphorylation modulates the PIP2 sensitivity of the CaM-SK channel complex."
25108821
rlimsp
"The availability of alternative substrates for AMPKK and CaMKIK allowed the unequivocal demonstration that AMP and Ca2+/calmodulin promote the activation of AMPK and Ca2+/calmodulin promote the activation of AMPK and CaMKI, respectively, via three independent mechanisms: 1) direct activation of AMPK and CaMKI, 2) activation of AMPKK and CaMKIK, and 3) by binding to AMPK and CaMKI, inducing exposure of their phosphorylation sites."
7592975
rlimsp
"The enzyme activation induced by the pH increase up to 8.2 does not affect the enzyme interaction with calmodulin [2.14 +/- 0.58 mol calmodulin per mol of (alpha beta gamma delta)]; Kd = (4.14 +/- 1.22).10(-8) M. However, the enzyme activation during its autocatalytic phosphorylation eliminates this effect practically completely."
2096954
rlimsp
"A magnetic dependence has been previously reported by others in an homologous system, CaM dependent myosin light chain phosphorylation, implying that there may be an underlying magnetic interaction that involves the initial Ca(2+)/CaM binding process common to both enzymatic pathways."
12483663
sparser
"In addition, no AngII-stimulated phosphorylation of CAM was observed, whereas there was a strong stimulation of AT 1 WT phosphorylation."
19061936
sparser
"It has been suggested that a fraction of CAM is constitutively phosphorylated in vivo [109,110] ."
16765984
rlimsp
"Calcium-activated, phospholipid-dependent phosphoproteins are present in human placental cytosol; whether calcium-activated, calmodulin-dependent phosphoproteins also are present remains a question."
3714718
sparser
"In vitro, CAM can be phosphorylated by casein kinase-II (CKII) at Thr79, Ser81, Ser101 and less frequently at Thr117, as well as by MLCK at Thr26 and also less frequently at Thr29."
16765984
rlimsp
"Autothiophosphorylated CaM-kinase II was resistant to autodephosphorylation."
11401530
sparser
"Furthermore, CSC (25 microgram/ml) both increases the rate of transendothelial migration of vitamin D3-differentiated monocyte-like cells across the HUVEC monolayer by 200% and causes an approximately 10-fold increases in the phosphorylation of platelet endothelial CAM (PECAM-1), an adhesion molecule located at intercellular junctions and involved in endothelial cell-cell adhesion."
8928867
rlimsp
"Calmodulin (CaM) is phosphorylated in vitro and in vivo by multiple protein-serine/threonine and protein-tyrosine kinases."
12153558
sparser
"CaMKII is believed to phosphorylate CAM in vivo but its regulation and functional significance is not yet known."
16765984
sparser
"Phosphorylation of both ANXA2 (aa17_29) and ANXA1 (aa14_26) showed increased, whereas phosphorylation of CALM (aa95_107) significantly decreased by FVP, compared to untreated control cells ( xref , indicated with arrows)."
34223559
sparser
"It is noteworthy that CAM is not phosphorylated by other Ser/Thr kinases such as PKA, PKC and casein kinase-I, which indicates that CAM is not under direct control by the autonomic nervous system."
16765984
sparser
"Cam-PK Phosphorylation of phospholamban following chymotryptic digestion of cardiac SR There are at least two explanations that could account for the decreased level of Cam-PK-catalyzed phosphorylat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
8323944
sparser
"Interestingly, the phosphorylation of CAM and CAMKII was significantly increased within 30 min after BzATP stimulation, and the phosphorylation of CAMKIV peaked within 10 min ( Fig. 6 A)."
35074408
sparser
"Phosphorylation of the CAM in its basal state supports the hypothesis that the A293E mutant structurally mimics the agonist-occupied wild-type receptor and, thus, serves as a substrate for GRK2."
11337028
sparser
"The Cam-PK phosphorylations and calmodulin-affinity- labeling were performed as described in Materials and Methods."
8323944
sparser
"Furthermore, at the biochemical level, phosphorylation of another key CAM gene, phosphoenolpyruvate carboxylase, by phosphoenolpyruvate carboxylase kinase displays circadian rhythms in constant darkness ( xref )."
30546378
sparser
"A further study was shown that non-phosphorylated CALM only interacts with the EGFR when is not phosphorylated at Tyr 1173 (tyrosine 1173 ) [ xref ]."
33602237
sparser
"328 Volume 155, number 2 FEBS LETTERS May 1983 Table 1 Phospholamban phosphorylation and oxalate-supported Ca^-uptake in mixed membrane preparations of embryonic, developing and adult [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
6852244
sparser
"All reactions were monitored by thin layer chromatography (TLC) carried out on either Merck silica gel plates (0.25 mm thick, 60F254) or Millipore Silica gel aluminum sheets (60F254) and visualized by using UV (254 nm) or dyes such as KMnO 4 , p -anisaldehyde, and CAM (Hannesian’s Stain)."
27689912
rlimsp
"This investigation provides important insights into how the increased electronegativity of a phosphorylated CaM protein affects the binding, dynamics, and activation of the NOS enzymes."
22486744
sparser
"All reactions were monitored by thin layer chromatography (TLC) carried out on Merck silica gel plates (0.25 mm thick, 60F254), visualized by using UV (254 nm) or dyes such as KMnO 4 , p -Anisaldehyde and CAM."
22449031
rlimsp
"Calmidazolium and compound 48/80 inhibit calmodulin-dependent protein phosphorylation and ATP-dependent Ca2+ uptake but not Ca2+-ATPase activity in skeletal muscle sarcoplasmic reticulum."
6233277
sparser
"All reactions were monitored by thin layer chromatography (TLC) carried out on Merck silica gel plates (0.25 mm thick, 60F254), visualized by using UV (254 nm) or dyes such as KMnO 4 , p -Anisaldehyde and CAM (Cerium Ammonium Molybdate or Hanessian's Stain)."
34902246
rlimsp
"Day and night forms of phosphoenolpyruvate carboxylase (EC 4.1.1.31) (PEPC) were extracted from leaves of the CAM plants Kalanchoe daigremontiana, K. tubiflora and K. blossfeldiana previously fed with [32P] labelled phosphate solution. A one-step immunochemical purification followed by SDS polyacrylamide gel electrophoresis and autoradiography showed that, in all species, the night form of the enzyme was phosphorylated and not the day form. Limited acid hydrolysis of the night form and two-dimensional separation identified predominantly labelled phosphoserine and phosphothreonine. In vitro addition of exogenous acid phosphatase (EC 3.1.3.2) to desalted night form-containing extracts resulted within 30 min in a shift in PEPC enzymic properties similar to the in vivo changes from night to day form. It is suggested that phosphorylation-dephosphorylation of the enzyme could be the primary in vivo process which might explain the observed rhythmicity of enzymic properties."
3707571
sparser
"In no case, was affinity labeling more sensitive to proteinase digestion than was the endogenous Cam-PK-catalyzed phosphorylation of phospholamban; i.e., the Cam-PK phosphorylation of phospholamban wa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
8323944
rlimsp
"The phosphorylation of the calmodulin-dependent enzyme myosin light chain kinase, purified from bovine tracheal smooth muscle and human blood platelets, by the catalytic subunit of cAMP-dependent protein kinase and by cGMP-dependent protein kinase was investigated."
6547441
rlimsp
"Inhibition of human platelet secretion and of Ca2+, calmodulin-dependent protein phosphorylation by the antiallergic agent GMCHA."
3390216
sparser
"The current understanding of the exact pathophysiological mechanism and how it links to the structural components of calmodulin structure is not clear, however, it is likely related to the large sarcoplasmic Ca 2 + release channel RyR2 sharing the same phenotypic expression.[ xref ] Proof of concept studies using knock-in animal models and human-induced pluripotent stem cells are missing and are another area of research.[ xref ] A recent study making use of nuclear magnetic resonance was able to provide insight into the particular structural effects of CALM1 p."
38817258
rlimsp
"These results suggest that GMCHA, a potent inhibitor of histamine release from mast cells, suppresses platelet secretion, relaxes vascular smooth muscle and inhibits Ca2+, CaM-dependent protein phosphorylation, all at similar concentrations."
3390216
sparser
"After incubation of cultured hepatocytes with [32P]Pi, phosphorylated cell-CAM 105 could be isolated."
3092813
rlimsp
"Day and night forms of phosphoenolpyruvate carboxylase (EC 4.1.1.31) (PEPC) were extracted from leaves of the CAM plants Kalanchoe daigremontiana, K. tubiflora and K. blossfeldiana previously fed with [32P] labelled phosphate solution. A one-step immunochemical purification followed by SDS polyacrylamide gel electrophoresis and autoradiography showed that, in all species, the night form of the enzyme was phosphorylated and not the day form."
3707571
sparser
"Calmodulin is the receptor for calcium, calcium-calmodulin complex collaborates with different targets, Ca 2+ /calmodulin-dependent protein kinase (CaMK) is serine/threonine protein kinases activated [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33894359
sparser
"Thus, maintain the steady state but required level of the phosphorylated CAM on the plasma membrane."
14592788
rlimsp
"GMCHA also inhibits the Ca2+, CaM-dependent myosin light chain phosphorylation of human platelets."
3390216
sparser
"Furthermore, feruloyl amides (FAM) and p -coumaroyl amides ( p CAM) signals were observed in anomeric region."
37499927
sparser
"All reactions were monitored by thin layer chromatography (TLC) carried out on Merck silica-gel plates (0.25 mm thick, 60F254), visualized by using UV (254 nm) or dyes such as ninhydrin, KMnO4, p -anisaldehyde or CAM (ceric ammonium molybdate)."
29501941
sparser
"The binding might be controlled by e.g. phosphorylation of cell-CAM 105 and by interactions with other molecules in the cell cortex."
2917610
rlimsp
"In the presence of low calcium concentrations and in the absence of cytosol, the phosphorylation of CaM-BP103/97 is, on a molar basis, equal to or greater than that of proteins 4.1 and 4.9."
3771508
rlimsp
"However, it is not known whether this is due to direct activation, or calmodulin-dependent protein phosphorylation, or both."
1510133
sparser
"It is conceivable that once the required mass of CAM phosphorylation is complete, the remaining free [NTP] o in circulation would potentially be hydrolyzed by E-NTPase or by E-NTPDase in the circulati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
14592788
sparser
"Our phosphoproteomics analysis revealed hyperphosphorylation of Cam1, Cna1, Cnb1, and Crz1 in the cka1 ∆ mutant ( xref )."
38193728
sparser
"Expression convergence of the PPCK-mediated phosphorylation of PEPC in CAM plants."
36960529
rlimsp
"Their actions were analyzed with respect to activation of receptors, increase in the intracellular calcium concentration, and increase in calmodulin-dependent myosin light chain phosphorylation leading to contraction."
10776476