IndraLab

Statements

MIB2 ubiquitinates CYLD. 7 / 7
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"First, co-transfection of haemagglutinin epitope tagged-MIB2 (HA-MIB2) [XREF_BIBR], CYLD (untagged) and His 6 -ubiquitin expression constructs clearly drove the ubiquitylation of CYLD compared to cells transfected with CYLD and His 6 -ubiquitin, compare lanes 1 and 2), suggesting that MIB2 may ubiquitylate CYLD."
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"As we demonstrated that MIB2 can ubiquitylate itself as well as CYLD, we postulated that CYLD, as an ubiquitin hydrolase, might deubiquitylate MIB2, hence providing a potential mechanism of mutual regulation."
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ubibrowser
"UbiNet 2.0"
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"Taken together, these observations suggested that MIB2 ubiquitylates CYLD leading to CYLD destabilisation and degradation, and that CYLD might be involved in regulation of JAG2, and ultimately Notch signalling, via its interaction with MIB2."
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sparser
"First, co-transfection of haemagglutinin epitope tagged-MIB2 (HA-MIB2) [ xref ], CYLD (untagged) and His 6 -ubiquitin expression constructs clearly drove the ubiquitylation of CYLD compared to cells transfected with CYLD and His 6 -ubiquitin (Figure xref panel (i), compare lanes 1 and 2), suggesting that MIB2 may ubiquitylate CYLD."
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"Taken together these results suggested that CYLD interacts with and is ubiquitylated by MIB2."
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sparser
"Taken together, these observations suggested that MIB2 ubiquitylates CYLD leading to CYLD destabilisation and degradation, and that CYLD might be involved in regulation of JAG2, and ultimately Notch signalling, via its interaction with MIB2."
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