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IL6 phosphorylates STAT3 on Y705. 211 / 223
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"In this study, phosphorylation of STAT3 at Y705 induced by IL-6 was weakly inhibited by AG490, a Jak2 kinase inhibitor, but not by cirsiliol at 90muM in the serum starved condition (XREF_FIG)."
33392396
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"As shown, C188-9 dose-dependently inhibited the IL-6-induced phosphorylation of STAT3 at the Y705 ( xref )."
29410184
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"In contrast, phosphorylation of Stat3 Tyr705 by IL-6 was not inhibited by Bim-1."
24496235
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"It has been shown that both IL-10 and IL-6 could induce STAT3 phosphorylation at Tyr705."
24513872
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"Besides, homoharringtonine ( 36 ) could reversibly inhibit IL-6-induced STAT3 tyrosine 705 phosphorylation and reduced anti-apoptotic proteins expression."
34333418
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"Studies have shown that IL-6 induces STAT3 phosphorylation on both Tyr705 and Ser727 [ xref , xref ]."
27074565
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"In all three lines, exogenous addition of IL-6 induced phosphorylation of Stat3 Tyr705 ( xref ), which was inhibited following treatment with AZD1480."
19962667
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"As expected, IL-6 alone strongly phosphorylated STAT3 at Y705, but, surprisingly, did not phosphorylate S727."
29410184
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"Even more surprisingly, the co-treatment of VD3 and IL6 induced significant phosphorylation of STAT3 Tyr705 at 24 h post-treatment that was not induced by the single treatments (XREF_FIG)."
33618332
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"As expected, IL6 induced rapid phosphorylation of STAT3 Tyr705 within the first 30 minutes of treatment."
33618332
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"It has been shown previously that IL-6 stimulation leads to STAT3 phosphorylation at Tyrosine 705 (Y705) and Serine 727 (S727) which have been suggested to be required for dimerization and maximal transcriptional activity of STAT3."
27311882
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"Andrographolide was able to block IL-6-stimulated Stat3Tyr705 phosphorylation, Erk phosphorylation, and Akt phosphorylation ( xref )."
21442031
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"Our data show that andrographolide reduced not only the levels of Stat3Tyr705 in DU145 cells that express constitutive IL-6 autocrine loop, but also the levels of IL-6-induced Stat3Tyr705 phosphorylation in LNCaP cells treated with exogenous IL-6."
21442031
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"IL-6 induces STAT3 phosphorylation at Tyr 705 and Ser 727 through IL-6 receptors and JAK to activate STAT3 signalling in HCC cells."
26061710
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"We found that STAT3 (Y705) phosphorylation was significantly inhibited by IL-6 antibody or SH-4-54 pretreatment ( Fig. 2 D)."
28130110
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"It has been shown that both IL-10 and IL-6 could induce STAT3 phosphorylation at Tyr705 ( xref )."
24513872
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"IL-6-induced STAT-3 Y705 phosphorylation was increased in activated, but not in naive PTPN2-deficient CD4 + T cells ( Fig. 7B and C)."
27658548
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"In this study, we confirmed that IL-6 could phosphorylate STAT3 (Y705) (XREF_FIG) but this induction was inhibited by HHT treatment (XREF_FIG) and the STAT3 nucleus translocation and transcription activity were simultaneously repressed (XREF_FIG)."
26166037
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"As shown in XREF_FIG, after serum starvation, phosphorylation of Y705 in STAT3 was induced by IL-6 stimulation."
33392396
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"IL-10 and IL-6 can activate the phosphorylation of STAT-3 at the Tyr705 amino acid position through the activation of JAK [27]."
35765486
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"Interestingly, co-treatment of 5 μM sorafenib for 24 h dramatically increased IL-6 induced STAT3 phosphorylation at tyrosine-705, indicating IL-6 signaling was enhanced in the presence of sorafenib tr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34687791
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"Casticin suppressed the constitutive/IL-6 driven phosphorylation of STAT3 Y705 and did not affect phosphorylation at STAT3 S727 in the tested tumor cells [ 286 ]."
32325172
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"In chronic lymphocytic leukemia (CLL) cells, both interleukin-6 (IL-6) and the B-cell receptor (BCR) activate Janus kinase 2 (JAK2) and induce the phosphorylation of signal transduction and activator of transcription 3 (STAT3) on tyrosine 705 residues."
28722170
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"Furthermore, consistent with our previous finding, IRAK-1 deletion did not affect STAT3 Tyr705 phosphorylation induced by IL-6 ( xref )."
19380824
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"When activated by the IL-6 family of cytokines, receptor tyrosine kinases or JAKs, STAT3 is primarily phosphorylated at the Y705 site, after which it is transported to the nucleus where it acts as a transcription factor with oncogenic functions (29)."
35117228
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"IL-6 enhanced phosphorylation of Stat3 at tyrosine 705 (pY705) and serine 727 (pS727), whereas TGF-β and gefitinib suppressed Stat3 phosphorylation ( Fig. 1 B)."
28115165
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"A time-course of IL-6-promoted STAT3 Tyr 705 phosphorylation demonstrated that naringenin began to exert a significant inhibitory effect after approximately 3 h stimulation ( xref b)."
23782265
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"When the STAT3 Ser727Asp mutant was overexpressed, IL-6 stimulation was still required to induce STAT3 Tyr705 phosphorylation and dimerization, while the IL-6-induced transactivation was not significa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11322950
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"To determine whether AG490 and S3I-201 inhibit p-STAT3 (STAT3 activation) human LNCaP cells which do not constitutively express activated STAT3, IL-6 was exogenously added to induce the phosphorylation of STAT3 at Tyr705."
24520293
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"In LNCaP cells where STAT3 is not constitutively active, the exogenous addition of IL-6 induced the phosphorylation of STAT3 at Tyr705 which had been reduced by JAK and STAT3 inhibitors."
24520293
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"As shown in Fig. 4 B, the suppression of the IL-6-induced Tyr705 phosphorylation of STAT3 by resveratrol is significantly attenuated in the presence of L-NAME."
16150460
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"We found that IL-6 induced STAT3 phosphorylation at Y705, the STAT3 phospho-activation site, but not at S727 (the other site for transcriptional activation in STAT3) ( xref a–c)."
29270528
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"While E966-0530-45418 did not impact the IL6-induced phosphorylation of STAT3 at Y705, it effectively suppressed its phosphorylation at S727 in the nucleus and also led to a reduction in p-RNA polymerase II S2/5 levels (Figure xref C)."
39781457
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"AZD1480 inhibited IL-6−induced STAT3 phosphorylation at Y705 in the cells from IgAN patients (IgAN-PB and IgAN-tonsillar cells) and the control subjects (HC-PB and OSA-tonsillar cells) ( xref c and d, xref c and d)."
29270528
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"The IL-6-induced STAT3 phosphorylation at Y705 in IgAN-PB cells started to weaken after 1 hour, but was detected even after 48 hours ( xref a and b)."
29270528
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"Phosphorylation of STAT3 Y705 residue, for example, can be induced by interleukin 6 (IL-6) [20]."
36473131
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"Knockdown of STAT3 in human vascular endothelial cells using siRNA (knockdown efficiency 80-90%) abolished the phosphorylation of STAT3 Tyr705 caused by IL-6 trans-signaling."
30185178
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"These results demonstrate that activation of FGFR1 leads to production of IL-6 family cytokines, which act through gp130 to induce phosphorylation of STAT3 Tyr705."
24197137
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"Sanguinarine inhibits constitutive as well as IL6-induced phosphorylation of Stat3 at both Tyr705 and Ser727 in prostate cancer cells."
21538419
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"We demonstrate that IL-6 classic signaling causes transient phosphorylation of STAT3 Tyr705, while trans-signaling also leads to phosphorylation of AKT Ser473 and ERK1/2 Thr202 and Tyr204."
30185178
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"Phosphorylation of the STAT3 Y705 residue can be induced by cytokines (IL-6, IFN-alpha), growth factors (EGFR, EGFRvIII, HER2 and PDGFR) and non receptor tyrosine kinases (Src and all the JAK family proteins) [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
24743777
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"Besides, it is well documented that IL-6 phosphorylates transcription factor Stat3 at Tyr705 residue and has a role in inflammation [XREF_BIBR - XREF_BIBR]."
24078775
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"As expected, IL6 induced rapid phosphorylation of STAT3 Tyr705 within the first 30 minutes of treatment."
33618332
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"Interestingly, the inflammatory cytokine IL-6 could induce a robust physical STAT3-NFATc2 interaction that was further enhanced by addition of serum (XREF_FIG) and that required STAT3 Y705 phosphorylation (XREF_FIG)."
26823495
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"As shown in xref , treatment of DU145 cells with sanguinarine resulted in a significant decrease in the levels of both constitutive and IL-6-induced tyrosine 705 phosphorylation of Stat3."
21538419
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"Even more surprisingly, the co-treatment of VD3 and IL6 induced significant phosphorylation of STAT3 Tyr705 at 24 h post-treatment that was not induced by the single treatments ( xref )."
33618332
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"Our results showed that IL-6 stimulated STAT3 phosphorylation on tyrosine 705 in DLD1 and Hep3B cancer cells."
26883202
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"IL-6 overexpression is also commonly observed in GBs and demonstrated to induce STAT3 phosphorylation at Tyr-705 through the hexameric receptor complex IL-6Rα binding [10,36]."
31698775
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"Activation of STAT3 occurs through ligand binding to cell-surface receptors, for example, the interleukin 6 (IL-6) receptor, leading to phosphorylation of STAT3 at the tyrosine 705 residue via kinases such as JAK, c-Src, and c-Abl."
37511611
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"Because in unstimulated MM-1 cells STAT3 is not activated, we incubated the cells with IL-6 that induces phosphorylation of STAT3 on tyrosine 705 residues."
27076684
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"Further analysis of the mechanisms driving STAT3 phosphorylation demonstrated that FGFR activation induces various IL-6 family members, which contributed to phosphorylation of STAT3 Tyr705."
24197137
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"In LNCaP cells where Stat3 is not constitutively active, exogenous addition of IL-6 induced phosphorylation of Stat3 at Tyr705, which was reduced by sanguinarine ( xref )."
21538419
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"IL-6 mediates tyrosine phosphorylation of STAT3 at tyrosine 705 (Y705) which results in dimerization and translocation to the nucleus."
34953891
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"Both autocrine and paracrine IL-6 can lead to constitutive phosphorylation of STAT3 at Tyr705 and Ser727 residues; IL-6 from paracrine sources (such as cancer-associated myeloid cells or fibroblasts) could induce autocrine IL-6 production and STAT3 phosphorylation in tumor cells (9)."
33313091
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"Furthermore, it was previously shown that breast cancer tumor cells secrete IL-6, which causes STAT3 Y705 phosphorylation in the lymphatic endothelial cells of lymphatic vessels."
36497411
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"DDIAS depletion attenuated IL-6–induced STAT3 phosphorylation at Y705 but not at S727 in non-small cell lung cancer (NSCLC) cells, such as NCI-H23 and NCI-H1703 (Fig. 1b)."
31900385
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"Stattic inhibited IL-6−induced STAT3 phosphorylation at Y705 in a dose-dependent manner in PBMC- and tonsil-derived cells from IgAN patients and in those from the control subjects ( xref c and d; xref c and d)."
29270528
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"The results disclosed that PRMT6 overexpression amplified IL-6-induced Y705 phosphorylation in STAT3 WT, yet this effect was nullified in STAT3 R729K mutant, despite the latter having lower basal Y705 phosphorylation levels than STAT3 WT in normal culture conditions (Fig. xref and Supplementary Fig. xref )."
37813837
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"When applied together with sIL-6R, very low concentrations of IL-6 (i.e. 1ng/ml) were sufficient to induce STAT3 Tyr705 phosphorylation (Additional file 3 : Figure S3), while higher concentrations (i.e. 50ng/ml) were required to induce similar degree of phosphorylation when IL-6 is added without sIL-6R."
30185178
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"At 48 h after transfection, exposure of exogenous IL-6 in scrambled siRNA-transfected A549 cells induced tyrosine 705 phosphorylation in a dose-dependent manner, and IL-6-induced STAT3 Tyr705 phospho[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23583378
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"We found that the effect of nm23-H1 depletion on IL-6-induced STAT3 Tyr705 phosphorylation was reversed by ectopic expression of shRNA-resistant nm23-H1 protein."
23583378
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"Furthermore, HMC was also able to suppress the IL-6 induced phosphorylation of STAT-3 at serine 727 and tyrosine 705 residues in K-562 cells ( Fig. 6 D)."
26291658
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"Consistently, knockdown of TRIM27 markedly inhibited IL-6-induced phosphorylation of STAT3 at Y705 (Fig.  xref )."
30143645
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"TRIM27 deficiency also markedly inhibited IL-6-induced phosphorylation of STAT3 Y705 but not JAK1 Y1022/1023 (Fig.  xref )."
30143645
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"Therefore, both K685 acetylation and Y705 phosphorylation of STAT3 induced by IL-6 contribute to its activation in the regulation of Fra-1 gene expression."
25750173
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"Whereas IL-6 induced STAT3-Tyr705 phosphorylation within a few minutes, the stimulation of the BCR with anti-immunoglobulin M (IgM) antibodies required a few hours, since it involves the transcriptional activity of NF-κB. Remarkably, prolonged incubation with anti-IgM antibodies induces transcription and secretion of IL-6, further promoting the phosphorylation of STAT3 on tyrosine residues."
31817171
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"IL-6, which phosphorylates STAT3-Y705, is an anorexigenic agent in the hypothalamus and decreases hypothalamic Npy and Agrp mRNA levels in mouse, while IL-6 deficiency has limited effects ( Pereda et [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38387703
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"Localization of STAT3 to mitochondria increased after inducing STAT3 phosphorylation on tyrosine 705 by IL-6 (10 ng/ml)."
29702530
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"IL-6 triggers JAK2-dependent phosphorylation of STAT3 Y705, which facilitates STAT3 dimerization and translocation into nucleus to initiate the transcription of a wide spectrum of genes 8. Indeed, knockdown of STAT3 largely abolished Glut5 expression in the IL-6-treated DU145 and HSC-3 cells (Figure 2D)."
35813468
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"IL-6 increases STAT3 activation by phosphorylating STAT3 on tyrosine 705 and/or serine 727 in a broad range of human malignancies at the probability of 50% to 90% (Jiang et al., 2011; Wang et al., 201[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26101800
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"While IL-6 did induce Y705 phosphorylation of STAT3 ( xref ), it did not strongly induce STAT1 phosphorylation ( xref ), or induce ISG expression, as measured by immunoblot."
34537236
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"In our study, we showed that treatment with LPS or IL-6 alone markedly increased the phosphorylation of STAT3 at Tyr 705 and that CCM111 robustly decreased the phosphorylation of STAT3 at Tyr 705 in a dose- and time-dependent manner in macrophage cells or epithelial cells (Figs  xref and xref )."
28687744
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"Studies have shown that IL-6 induces STAT3 phosphorylation on both Tyr705 and Ser727 [XREF_BIBR, XREF_BIBR]."
27074565
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"Besides, it is well documented that IL-6 phosphorylates transcription factor Stat3 at Tyr705 residue and has a role in inflammation [ xref – xref ]."
24078775
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"CPT or a STAT3 siRNA significantly suppressed constitutive and IL-6-induced phosphorylation of STAT3 Tyr705, decreasing the levels of proteins encoded by STAT3-target genes (Bcl-xL, Mcl-1, survivin, and XIAP)."
28222632
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"The IL-6 induction of STAT3 (Y705) phosphorylation was suppressed by 103% (p =.0003) with C188-9 treatment and was 50% lower (p =.01) than that of control myotubes (XREF_FIG)."
30918582
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"In DDIAS-knockdown cells, PTPRM knockdown recovered the decrease in IL-6–mediated STAT3 Y705 phosphorylation."
31900385
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"IL-6 treatment (150 ng/ml, 30 min) of cultured epididymal adipose tissue (eWAT) led to an ~ 3-fold increase in the tyrosine 705 phosphorylation of STAT3."
22844518
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"STATTIC (80 μM), a small molecule inhibitor that binds the STAT3 SH2 domain to inhibit phosphorylation [ xref ], abolished IL-6 induced STAT3 Y705 phosphorylation following IL-6 treatment ( xref )."
21619877
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"We found that EB inhibited the IL-6-induced STAT3 Tyr705 phosphorylation in a dose and time-dependent fashion ( xref )."
26010889
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"As shown in xref A,B, the result demonstrated that BBM not only dose-dependently suppressed the phosphorylation of STAT3 at tyrosine 705 (Y705) induced by IL-6 but also decreased the nuclear translocation of STAT3."
33015502
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"Phosphorylation of STAT3 at Tyr705 induced by IL-6 is inhibited by sorafenib."
20371721
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"In cardiac myocytes, IL-6 treatment increases phosphorylation of STAT3 at the Tyr705 through the enhanced de novo synthesis of iNOS protein, leading to increased NO production and decreased cardiac contractility [XREF_BIBR]."
27995459
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"The IL-6 induction of tibialis anterior muscle gp130 expression, STAT3 (Y705) phosphorylation, and ERK1/2 phosphorylation were blocked by muscle gp130 loss (XREF_FIG)."
30918582
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"These data indicate that chalcone regulates both IL-6-induced STAT3 tyr705 phosphorylation and LPS-induced NF-κB activation in a time- and dose-dependent manner."
17320913
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"Blocking of IL-6 and IL-6 receptors using monoclonal antibodies effectively prevented IL-6/tyrosine phosphorylation (Tyr 705 ) of STAT3 proteins in Colo 205 cells [1] ."
20851386
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"The activation of numerous cytokines and molecules like the anti-inflammatory IL-10, proinflammatory interferon (IFN), interleukin-6 (IL-6), and lipopolysaccharide (LPS), can phosphorylate STAT3 at Tyr705 and induce dimerization and nuclear translocation of STAT3."
31803051
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"Phosphorylation of transcription factor STAT3 at Ser727 is associated with MAPK8 and MAPK1/3, whereas STAT3 phosphorylation at Tyr705 is mainly mediated by IL-6 and Janus kinases (JAK)."
29636527
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"CPT inhibits interleukin 6 (IL6)-induced phosphorylation of STAT3 Tyr705 in gastric cancer cells."
28222632
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"Previous studies have indicated that the IL-6 / gp130 and JAK signaling pathway can phosphorylate STAT3 Tyr705 in various tumor cells, including HCC [XREF_BIBR, XREF_BIBR]."
24655440
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"Indeed, the IL-6-STAT3 axis is a well-known trigger of muscle wasting in tumor-bearing mice.38 39 40 41 In agreement, in C2C12 myotubes, C26 CM, as well as IL-6, strongly induced phosphorylation of STAT3 at Tyr705 (pSTAT3) (Figures S1J and S1K)."
38052214
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"However, the depletion or deletion of AnxA2 had no effect on IL6-induced Tyr-705 phosphorylation of STAT3 (Supplementary Fig.  xref )."
| PMC
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"Analogously, culture of OPM2 cells with CM or IL-6 sharply increased STAT3 Y705 phosphorylation but had lesser effects on S727 ( xref , xref )."
34782371
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"As commonly reported in the literature, 2 h of IL-6 elevated phosphorylation of STAT3-Y705 while not altering total STAT3 levels ( Fig. 2 D)."
38387703
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"Here we show that CPT significantly inhibited constitutive and IL-6-induced phosphorylation of STAT3 Tyr705 in human gastric cancer cells without altering the phosphorylation of STAT3 Ser727 (pSer727), JAK1, and JAK2."
28222632
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"IL-6 leads to phosphorylation of STAT3 at tyrosine 705 and its subsequent activation and nuclear translocation via serine 727 phosphorylation."
22079905
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"It could displace the specific STAT3-SH2 binding peptide in vitro, had the dose-dependent inhibitory action on the phosphorylation of STAT3 at Tyr-705 triggered by IL-6, and was able to disrupt the mitochondrial oxidative phosphorylation (OXPHOS) pathways."
38854722
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"Cavin-1 limits IL-6-stimulated Tyr705 STAT3 phosphorylation."
29330478
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"As shown in Fig. 2 A, IL-6 activated phosphorylation of STAT3 (Tyr705), and the response peaked at 15 min, then decreased."
29857127
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"IL-6 is known to induce the phosphorylation of Tyr705 residue of STAT3 protein in two myeloma U266B1 and PCM6 cells; PP inhibited IL-6 (3 ng/mL)-induced STAT3 Tyr705 phosphorylation of both U266B1 and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22210382
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"Figure xref B shows that IL-6 stimulates phosphorylation of Tyr705 of STAT3 and expression of survivin in serum-starved A549 cells."
24661007
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"Further report dictated that mesenchymal stem cells, present in bone marrow, promote bone metastasis of ER positive breast cancer cells through IL-6 dependent phosphorylation of STAT3 at Tyr705 positi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26945971
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"To assess whether sunitinib also inhibits the phosphorylation of STAT3 at Tyr705 induced by IL-6, tumor cells were treated with sunitinib for 20 min, followed by the addition of IL-6 (10 ng/ml) for 10 min."
20053726
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"Immunoblotting assays showed that sunitinib greatly inhibited the phosphorylation of STAT3 (Tyr705) induced by IL-6 in primary cultures from medulloblastoma (XREF_FIG)."
20053726
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"Therefore, both K685 acetylation and Y705 phosphorylation of STAT3 induced by IL-6 contribute to its activation in the regulation of Fra-1 gene expression."
25750173
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"As expected, phosphorylation of STAT3 at both Ser727 and Tyr705 was induced by IL6 treatment."
24687132
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"IL-6 triggers JAK2-dependent phosphorylation of STAT3 Y705, which facilitates STAT3 dimerization and translocation into nucleus to initiate the transcription of a wide spectrum of genes xref ."
35813468
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"As expected, IL6 treatment led to phosphorylation of STAT3 on Tyr705."
24687132
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"IL-6 activates the JAK and STAT signaling pathway and leads to phosphorylation of Tyr 705 of the STAT3 protein [XREF_BIBR]."
22236378
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"Previous studies from our laboratory have demonstrated that the IL-6-induced phosphorylation of STAT3 on tyrosine 705 is crucial for ICAM-1 gene induction ( Wung et al., 2005 )."
16150460
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"In addition, intracisternal injection of IL-6 by itself significantly increased phosphorylation of STAT3 at Tyr705 and Ser727."
16413512
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"ALT inhibits IL-6-induced STAT3 tyrosine 705 phosphorylation."
33828598
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"IL-6 stimulation leads to phosphorylation of STAT-3 (Tyr 705), and inhibition of STAT-3 by specific inhibitors or siRNA abrogates IL-6-induced invasion and integrin beta6 upregulation, indicating the participation of this classical signaling cascade in response to IL-6."
32855767
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"Withaferin A (WFA), a natural product derived from Withania somnifera , potently induces cell death in high-risk drug-resistant pediatric neuroblastoma (NB) and MM cells by inhibiting IL-6-induced pho[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35868477
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"As a result, 3-DSC exhibited inhibitory effects on IL6-induced STAT3 phosphorylation at the Tyr705 residue at lower concentrations (0.1 and 0.3 μM), but STAT3 phosphorylation was unchanged at a higher concentration of the compound ( xref )."
27795451
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"IL-6 alone, IL-6 plus sIL-6R and LIF increased the phosphorylation of STAT3, most strongly at tyrosine 705 (tyr705) residue and to a milder degree at ser727 residue."
19267906
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"Previous studies from our laboratory have demonstrated that the IL-6-induced phosphorylation of STAT3 on tyrosine 705 is crucial for ICAM-1 gene induction in ECs ( Wung et al., 2005a,b )."
16413037
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"IL-6-induced STAT3 phosphorylation at Y705 was more intensive and persisted longer in cells from IgAN patients compared with those from control subjects [101] ."
35527168
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"As expected, we found that 50 ng/mL IL-6 activated STAT3 and induced STAT3 tyrosine phosphorylation on Tyr 705 in A549 and HCC827 cells."
24789104
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"In line with above described data, Western Blot analysis showed that DCE and CS decreased, in a dose-dependent manner, IL-6-induced tyrosine 705 phosphorylation of cytosolic STAT3 without affecting the total amount of STAT3 protein ( xref )."
21625597
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"Following ligand-induced dimerization of IL-6 receptor, the associated protein tyrosine kinases JAK1 and JAK2 are activated and subsequently induces STAT3 tyrosine-705 phosphorylation, the active form[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31479716
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"When HepG2 cells were held at 15 °C, the rate of IL-6 induced STAT3 Y705 phosphorylation was noticeably delayed ( xref )."
21619877
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"However, at a dosage of 50 μM, garcinol blocked the JAK-STAT pathway, lowering STAT3 (Y705) phosphorylation that was both constitutive and caused by IL-6."
38103726
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"It has been shown that IL-6-induced JAK-STAT3 signaling pathway activation plays important roles in endothelial cell activation [ xref ] that is mainly mediated by IL-6-induced Tyr705 phosphorylation in STAT3 [ xref ], which resembles our data on B7-H4 regulation by GM-CSF-induced Tyr705 phosphorylation of STAT3 in neutrophils."
33763491
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"It exhibited a dose dependent cytotoxic effect and inhibition of cell cycle progression in colon cancer cells by down regulating phosphorylation of ERK, JNK and p38 and IL-6 induced phosphorylation of[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27940056
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"IL-6 derived from PTFs greatly activated STAT3 Tyr705 phosphorylation in both non-tumor L-02 cells and HCC cells."
31118769
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"IL-6 induces STAT3 phosphorylation at Tyr-705 and Ser-727 through IL-6 receptors and JAK to activate STAT3 signalling in HCC cells."
26061710
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"This finding was confirmed in our study, in which the I/R increased phosphorylation of Akt (Ser473) and STAT3 (Tyr705) was further enhanced by IL-6."
33428604
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"Consistent with the above human T cell data, IL-10 and IL-21, but not IL-6, induced STAT3 phosphorylation at both Tyr705 (pY705) and Ser727 (pS727) sites in CD8 TILs from both B16-OVA and E.G7 tumors (Fig. 3, B and C)."
36688918
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"In this regard, IL-6, an essential pro-Th17 cytokine, induces phosphorylation of STAT3 on Tyr705 (STAT3 ), which is critical for Th17 differentiation."
39846842
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"In this study, phosphorylation of STAT3 at Y705 induced by IL-6 was weakly inhibited by AG490, a Jak2 kinase inhibitor, but not by cirsiliol at 90 μM in the serum-starved condition ( xref )."
33392396
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"IL-6 alone stimulates transient STAT3 Tyr705 phosphorylation, while PTFs induce long-lasting STAT3 activation by continuously secreting IL-6."
31118769
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"Again, rrCNTF failed to induce STAT3 phosphorylation in rat microglia while IL-6 stimulated strong phosphorylation of STAT3 tyr705 (Fig XREF_FIG)."
19267906
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"In cell assays, IL-6 and sIL-6R stimulation time-dependently promoted the activation and phosphorylation at site Tyr705 of STAT3 in RA-FLS, with a peak at 40min."
33594167
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"Phosphorylation of STAT3 Tyr705 (pSTAT3 Tyr705 ) was induced by OSM, LIF, CT-1, IL-11, and IL-6 ( xref )."
35163371
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"Further experiment revealed that the IL-6 and STAT3 pathway was diminished in the resistant cell lines (XREF_FIG), as exogenous IL-6 induced less STAT3 Tyr705 phosphorylation in the cisplatin resistant than in the cisplatin- sensitive parental cell lines."
27108527
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"For example, in the case of JAK1 and JAK2, IL-6 receptors are associated with the cytoplasmic tail of gp130 and further phosphorylate STAT3 at tyrosine 705; the activated STAT3 dimers then translocate from the cytosol to the nucleus [18,21]."
28001439
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"As shown in Figure XREF_FIG A, B, activation of IL-6 classic signalling in podocytes by IL-6 significantly increases the phosphorylation of STAT3 on Tyr 705 residue and leads to podocyte damage."
28881473
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"In the IL6-STAT3 signaling pathway, IL6 triggers STAT3 phosphorylation at its Y705 site."
39747894
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"Previous studies from our laboratory have demonstrated that IL-6-induced phosphorylation of STAT3 on tyrosine 705 is crucial for gene induction in ECs ( Wung et al., 2005a,b )."
16413037
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"IL-6 is known to stimulate STAT3 phosphorylation on Tyr705 in many cancer cells."
29606890
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"Furthermore, consistent with our previous finding, IRAK-1 deletion did not affect STAT3 Tyr705 phosphorylation induced by IL-6 (XREF_FIG)."
19380824
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"Recombinant IL-6 (10 ng/mL) increased STAT3-Tyr705 phosphorylation in eight of the nine ERα-positive cell lines evaluated by Western blot analysis ( xref and xref )."
26893580
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"These data indicate that 15d-PGJ2 regulates IL-6-induced STAT3 tyr705 phosphorylation in a time- and dose-dependent manner."
16413037
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"IL-6 alone stimulates transient STAT3 Tyr705 phosphorylation, while PTFs induce long-lasting STAT3 activation by continuously secreting IL-6."
31118769
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"In this study, we found that in vivo amlexanox treatment resulted in an IL-6–dependent Tyr705 phosphorylation of STAT3 (pY705 STAT3) in adipose tissue macrophages (ATMs)."
36449000
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"In both naïve and antigen-stimulated (“primed”) CD4+ T cells, loss of MeCP2 did not alter the abundance or activity of STAT3 protein; however, it did dampen the IL-6–dependent phosphorylation of Tyr 705 of STAT3, the hallmark of STAT3 activation ( xref )."
24619648
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"Phosphorylation of STAT3 at Tyr705 induced by IL-6 is inhibited by sorafenib."
20371721
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"In parallel, stimulation of cells with IL-6 induced STAT3 Y705 phosphorylation after 30 minutes at the earliest time point and resumed after 48 hours ( Supplemental Figure S3 E)."
23438478
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"The IL-6 induced constitutive phosphorylation of STAT3 Y705 was effectively suppressed by raloxifene in a dose-dependent manner (Fig.  xref b)."
32940862
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"CPT inhibits interleukin 6 (IL6)-induced phosphorylation of STAT3 Tyr705 in gastric cancer cells."
28222632
sparser
"The results identified that PPPF or STAT3 siRNA inhibits both constitutive and IL-6-induced Tyr705 phosphorylation of STAT3 in HepG2 cells."
28634059
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"Pretreatment of ECs with Mag dose dependently inhibited IL-6-induced Tyr705 and Ser727 phosphorylation in STAT3 without affecting the phosphorylation of JAK1, JAK2, and ERK1/2."
16520748
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"DDIAS depletion attenuated IL-6–induced STAT3 phosphorylation at Y705 but not at S727 in non-small cell lung cancer (NSCLC) cells, such as NCI-H23 and NCI-H1703 (Fig. xref )."
31900385
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"Western blotting results revealed that IL-6 as an activator of the STAT3 signaling pathway enhanced STAT3 phosphorylation at Tyr705 (Fig. 9) in both MDA-MB-231 and SKOV3 cells, without affecting the total STAT3 level."
38505673
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"IL-6 induced phosphorylation of STAT3 at both Tyr705 and Ser727 (Fig. xref and xref )."
26417930
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"The WB results showed that IL-6 activated the phosphorylation of STAT3 (Y705) and increased the MMP9 mRNA expression in ImKC educated by ANGTPL1-Exo in 24h."
33413536
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"TGR5 activation by both 23(S)-mCDCA and GPBARA suppresses IL-6-induced phosphorylation of STAT3 at Tyr705 by about 36% and 38%, respectively (Fig. xref ), but not at Ser727 ( xref )."
26417930
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"In line with above described data, Western Blot analysis showed that the compounds 9a , 9b and 9c decreased, in different grade, IL-6-induced tyrosine705 phosphorylation of cytosolic STAT3 without aff[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23287056
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"We observed that IL-6 at the concentrations from 10 to 100 ng/ml obviously enhanced pSTAT3(Y705) phosphorylation in both A2058 and A375 cell lines ( Figure S10 )."
35096622
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"Surprisingly, the IL-6 stimulation in the RhoC knockdown cell lines phosphorylates STAT3 tyr 705; this strongly suggests that phosphorylation of STAT3 by RhoC can take place via IL-6."
24533098
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"Next, to determine if STAT3 activation is sufficient to induce FTO-regulated ISGs, we treated Huh7 cells with IL-6, which induces Y705 phosphorylation of STAT3 [30]."
34537236
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"In the IL6-STAT3 signaling pathway, IL6 triggers STAT3 phosphorylation at its Y705 site."
39747894
reach
"TY treatment significantly reduced the IL-6- and IL-22-induced phosphorylation of STAT3 at Y705, a key residue for STAT3 activation (Fig. 4E)."
39833165
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"Curcumin was found to inhibit the proliferation of MM cells, constitutive and IL-6 induced phosphorylation of STAT3 Y705 , and IFNγ induced phosphorylation of STAT1 in MM cells."
32325172
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"These observations led us to the overarching hypothesis that negative regulation increases robustness of IL-6-induced STAT3-Y705 phosphorylation against varying STAT3 expression in individual cells."
| PMC
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"IL-6 increased Tyr705 phosphorylation of signal transducer and activator of transcription 3 (STAT3)."
19741197
sparser
"IL-6 strongly induced STAT3 phosphorylation at Y705 but not S727 and was inhibited by WFA after only 2 hours of incubation ( xref ); however, total STAT3 levels were reduced after exposure of cells to WFA for more than 4 hours, suggesting that WFA also downregulates STAT3 protein if cells are exposed to the drug for longer time periods."
25452693
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"We found that B6 had dose-dependent but differential inhibitory effects on the IL-6-induced STAT3 Tyr705 phosphorylation, IFN-γ-induced STAT1 Tyr701 phosphorylation (Fig.  xref ) and IFN-α-induced phosphorylation of both STAT1 and STAT3 (Fig.  xref ), which was consistent with the luciferase assay results."
31201357
reach
"IL-6 rapidly activated STAT3 (Y705) phosphorylation within 15 min (XREF_FIG), corresponding to an acute rise in STAT3 genes IL-6 and SOCS3 (XREF_FIG)."
22698915
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"In eight of nine ERalpha positive breast cancer cell lines, recombinant IL-6 increased phosphorylation of tyrosine 705 of STAT3."
26893580
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"IL-6 and OSM stimulated phosphorylation of STAT3 Y705 ."
36495330
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"Thus, IL-6 classical and trans-signaling have opposite effects to PMA on resting T cells; IL-6 increases STAT3 (Tyr705) phosphorylation, whereas PMA increases phosphorylation of the three other phosphosites."
35566660
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"Indeed, IL-6 treatment induced Y705 phosphorylation of STAT3 in a time dependent manner concomitantly inducing FTO protein expression with a maximal effect at 6 hours of treatment, suggesting that FTO could be a target gene of STAT3 in hepatocytes."
24410832
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"As shown in Fig. 4 , 10 ng/ml IL-6 stimulated the phosphorylations of Tyr-705 and Ser-727 of STAT3 in Hep3B cells, which was blocked by 100 nM PEDF."
16876827
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"However, IL-6 receptor activation induces the phosphorylation of both the Y705 and S727 residues of STAT3, and S727 phosphorylation is required to achieve maximal STAT3 transcriptional activity."
29082496
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"On the other hand, BM-MSCs aid in EMT of LM tumor cells in the primary site through IL-6-induced phosphorylation of the Y705 residue on the STAT3 molecule."
33262462
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"All four IL-6 signals caused highly significant increases in STAT3 (Tyr705) phosphorylation, a phosphosite present on both α and β STAT3 isoforms [13]."
35566660
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"As can be seen in XREF_FIG, niclosamide inhibited interleukin 6 induced phosphorylation of STAT3 Y705 phosphorylation in a dose dependent manner with 2mu completely inhibiting detection of phosphorylated Y705."
22829072
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"In our study, IL-6 could not induce STAT3 Tyr705 phosphorylation in the A549 shMRE11 cells (Figure 1A)."
36547079
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"Western blot analysis demonstrated that compound 25 obviously inhibited IL-6-induced STAT3 Y 705 phosphorylation in Hela and MDA-MB-231 cell at a concentration of 10 µM ( xref A,B)."
37240202
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"RA inhibits interleukin-6 (IL-6)-induced STAT3 Tyr 705 phosphorylation in chemosensitive and chemoresistant OS cell lines."
30745853
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"Kaempferol targeted IL-6-induced phosphorylation of STAT3 at Tyr 705 and p65 subunit of NF-κB at Ser 536 for the attenuation of COX-2 expression."
28955792
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"Phosphorylation of STAT3 (Y705) induced by IL-6 was greatest in naive T cells."
26912317
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"In one example, HOS depleted STAT3 levels in H4IIR rat hepatoma cells through ubiquitin-mediated proteosomal degradation, and subsequently decreased IL-22- and IL-6-induced STAT3 Y705 phosphorylation [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24394455
sparser
"Western blot analysis demonstrated that compound 25 inhibited constitutive STAT3 Y705 phosphorylation in A549 and DU145 cell lines, and also inhibited IL-6-induced STAT3 Y705 phosphorylation in Hela and MDA-MB-231 cell lines."
37240202
reach
"IL-6 strongly induced STAT3 phosphorylation at Y705 but not S727 and was inhibited by WFA after only 2 hours of incubation (XREF_FIG); however, total STAT3 levels were reduced after exposure of cells to WFA for more than 4 hours, suggesting that WFA also downregulates STAT3 protein if cells are exposed to the drug for longer time periods."
25452693
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"We next tested whether SHP2 affects robustness of late IL-6-induced STAT3-Y705 phosphorylation."
| PMC
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"By contrast, we also observed that both IL-6 and LPS induced STAT3 phosphorylation at tyrosine 705 and enhanced acetylation at lysine 685 (XREF_FIG)."
27866206
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"In the present study, our data show that DHA and EPA suppress the IL6-induced Tyr705 phosphorylation of STAT3, which in turn inhibit the transcription activation of CPR gene."
25881314
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"Endogenous IL-6 functions as a potent activator of STAT3 and could restore the phosphorylation of STAT3 Tyr705 in BRM silencing PC cells and regulate phenotypic changes in these cells."
28602977
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"In the present investigation, we sought to determine for the first time the significance of STAT-3 phosphorylation on tyrosine 705, which is described to be transiently induced by IL-6 in CLL cells [XREF_BIBR]."
24883303
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"As a positive control we verified that IL-6, an established activator of JAK-STAT signaling, induced nuclear translocation and Y705 phosphorylation of Stat3 in the macrophages ( Fig. 3 C and S Fig. 2 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37806297
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"Alantolactone, a sesquiterpene lactone derived from Inula helenium , has been found to interact with the STAT3 SH2 domain at Arg595 and inhibit IL-6- or EGF-induced STAT3 phosphorylation at Tyr705, th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35868477
sparser
"By contrast, we also observed that both IL-6 and LPS induced STAT3 phosphorylation at tyrosine 705 and enhanced acetylation at lysine 685 ( xref )."
27866206
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"Localization of STAT3 to mitochondria increased after inducing STAT3 phosphorylation on tyrosine 705 by IL-6 (10 ng/ml)."
29702530
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"SHP2 increases robustness and information transfer of early IL-6-induced STAT3-Y705 phosphorylation and ensures independence of basal STAT3 phosphorylation from varying STAT3 expression."
| PMC
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"Notably, in response to IL6, wild-type STAT3 was far more pronouncedly phosphorylated at tyrosine 705 (Y705) than STAT3 R414Q or STAT3 R417Q."
24721162
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"Mechanisms that reduce IL-6-induced STAT3-Y705 phosphorylation such as expression of SOCS3 and STAT3-S727 phosphorylation on the one hand increase robustness of signalling against varying STAT3 expression."
| PMC
sparser
"Incubation with orthovanadate markedly increased IL-6-stimulated STAT3 Tyr705 phosphorylation, yet this was still markedly inhibited by A769662, indicating that AMPK-mediated inhibition is at the level of JAK activity rather than reduced STAT3 phosphatase activity ( xref )."
27840174
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"Overexpression of PASD1 enhanced both basal and IL-6-induced STAT3 phosphorylation at Y705, whereas knockdown of PASD1 had opposite effects."
26892021
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"We found that phosphorylation of STAT3 Tyr705 is induced by both IL-6 alone and in combination with sIL-6R (Fig.  xref , left) in a dose-dependent manner (Additional file xref : Figure S3)."
30185178
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"Because IL-6 is an activator of the JAK2 and STAT3 signaling pathway, exogenous IL-6 was used to induce the phosphorylation of STAT3 at Tyr705."
26580615
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"Phosphorylation at Tyr705 in STAT3 is induced by IL-6 and IL-23, involves the PI3K-Akt-mTORC1 pathway and controls Th17 commitment and function ( xref , xref )."
29237778
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"As a mediator of cellular inflammatory response, phosphorylation of the transcription factor STAT3 at Y705, which among others is induced by IL-6, was assessed [XREF_BIBR]."
25400510
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"In most HCC cell lines, IL-6 enhances STAT3-Y705 phosphorylation."
30606233
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"In these cells IL-6 was found to induce phosphorylation of STAT3 on Tyr 705 and this was effectively inhibited by both trans-resveratrol and flavone, despite the lack of SOCS3 expression (XREF_FIG d)."
23782265
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"IL-6 also increased the phosphorylation of STAT3 (Tyr 705), a known event in the activation of the JAK and STAT signaling cascade (XREF_FIG C)."
19502419
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"In BAEC, pretreatment for 30 min with the arachidonic acid metabolite and electrophile, 15-deoxy- Δ 12,14 –prostaglandin J 2 (15d-PGJ 2 ) inhibited IL-6-induced STAT3 Y705 phosphorylation, as did acrolein xref ."
22905257
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"We demonstrate that IL-6 classic signaling causes transient phosphorylation of STAT3 Tyr705 , while trans-signaling also leads to phosphorylation of AKT Ser473 and ERK1/2 Thr202/Tyr204 ."
30185178
sparser
"Hence, IL-6 is generally known to activate JAK/STAT3 pathway regardless of binding to soluble or membrane bound IL-6R. In line with this, we show that both classic- and trans-signaling of IL-6 trigger STAT3 Tyr705 phosphorylation in a dose- and time-dependent manner."
30185178