IndraLab

Statements

CAMK2_complex phosphorylates CYLD. 35 / 37
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rlimsp
"To confirm that CaMKII phosphorylates CYLD at the PSD directly rather than via sequential phosphorylation through activation of other kinases, phosphorylation of purified CYLD by purified CaMKII was tested."
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reach
"While CaMKII enzymatic activity does not appear to be necessary for CYLD recruitment to the PSD, CaMKII nonetheless can phosphorylate CYLD."
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rlimsp
"Two in vitro phosphorylation protocols were used to identify CaMKII-mediated phosphorylation sites on CYLD and test whether CaMKII phosphorylates CYLD directly."
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sparser
"CaMKII phosphorylates CYLD on multiple residues."
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sparser
"Among the three sites on CYLD phosphorylated by CaMKII, S-418 has been reported to be phosphorylated by IKK, resulting in an inhibition of CYLD activity xref , xref ."
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reach
"Since CYLD is a major protein in isolated PSDs XREF_BIBR and is phosphorylated by CaMKII, it is presumed that the observed changes in deubiquitination are a result of CaMKII mediated activation of CYLD."
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sparser
"Having established that CaMKII phosphorylates CYLD, we further tested the effect of CaMKII-mediated phosphorylation on its activity."
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sparser
"Purified CaMKII phosphorylates CYLD on at least three residues (S-362, S-418, and S-772 on the human CYLD protein Q9NQC7-1) and promotes its deubiquitinase activity."
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rlimsp
"Since CYLD is a major protein in isolated PSDs [20] and is phosphorylated by CaMKII, it is presumed that the observed changes in deubiquitination are a result of CaMKII-mediated activation of CYLD."
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reach
"Addition of ATP led to phosphorylation of the same three residues, indicating that CaMKII directly phosphorylates CYLD on these residues."
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reach
"Among the three sites on CYLD phosphorylated by CaMKII, S-418 has been reported to be phosphorylated by IKK, resulting in an inhibition of CYLD activity XREF_BIBR, XREF_BIBR."
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reach
"Activation of CaMKII in isolated PSDs promotes phosphorylation of CYLD on the same residues and also enhances endogenous deubiquitinase activity specific for K63 linked polyubiquitins."
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rlimsp
"CaMKII phosphorylates CYLD on multiple residues."
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reach
"Incubating purified CYLD and purified CaMKII promotes phosphorylation on the same serine residues, providing evidence that the phosphorylation of CYLD in the PSD fractions is directly mediated by the endogenous CaMKII."
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reach
"Together, these data indicate that CaMKII activation promotes phosphorylation of CYLD at the PSD."
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reach
"Activation of endogenous CaMKII in isolated PSDs promotes phosphorylation of CYLD."
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sparser
"CaMKII co-immunoprecipitates with CYLD and promotes phosphorylation of CYLD at the PSD."
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reach
"CaMKII phosphorylates CYLD on multiple residues."
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reach
"Having established that CaMKII phosphorylates CYLD, we further tested the effect of CaMKII mediated phosphorylation on its activity."
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sparser
"Addition of ATP led to phosphorylation of the same three residues, indicating that CaMKII directly phosphorylates CYLD on these residues."
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rlimsp
"Having established that CaMKII phosphorylates CYLD, we further tested the effect of CaMKII-mediated phosphorylation on its activity."
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rlimsp
"These results with purified proteins show that CaMKII activates CYLD via phosphorylation."
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sparser
"Two in vitro phosphorylation protocols were used to identify CaMKII-mediated phosphorylation sites on CYLD and test whether CaMKII phosphorylates CYLD directly."
24614225
sparser
"These results led us to further examine whether CaMKII promotes phosphorylation of CYLD at the PSD."
24614225
rlimsp
"While CaMKII enzymatic activity does not appear to be necessary for CYLD recruitment to the PSD, CaMKII nonetheless can phosphorylate CYLD."
24614225
rlimsp
"Addition of ATP led to phosphorylation of the same three residues, indicating that CaMKII directly phosphorylates CYLD on these residues."
24614225
reach
"Two in vitro phosphorylation protocols were used to identify CaMKII mediated phosphorylation sites on CYLD and test whether CaMKII phosphorylates CYLD directly."
24614225
reach
"These results led us to further examine whether CaMKII promotes phosphorylation of CYLD at the PSD."
24614225
rlimsp
"Among the three sites on CYLD phosphorylated by CaMKII, S-418 has been reported to be phosphorylated by IKK, resulting in an inhibition of CYLD activity [30], [31]."
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sparser
"To confirm that CaMKII phosphorylates CYLD at the PSD directly rather than via sequential phosphorylation through activation of other kinases, phosphorylation of purified CYLD by purified CaMKII was tested."
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sparser
"Consistent with mass-spectrometric results, Western immunoblots using an antibody specific for phospho CYLD (S-418) showed that purified CaMKII phosphorylates purified CYLD on this residue ( xref - left)."
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rlimsp
"CaMKII-mediated phosphorylation of CYLD enhances its deubiquitinase activity as shown in in vitro experiments using purified proteins."
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sparser
"While CaMKII enzymatic activity does not appear to be necessary for CYLD recruitment to the PSD, CaMKII nonetheless can phosphorylate CYLD."
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rlimsp
"Thus, CaMKII directly phosphorylates at least three residues on CYLD, while the identity of the kinase responsible for phosphorylating the additional fourth residue is unclear."
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reach
"To confirm that CaMKII phosphorylates CYLD at the PSD directly rather than via sequential phosphorylation through activation of other kinases, phosphorylation of purified CYLD by purified CaMKII was tested."
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