IndraLab
Statements
"Another function of reduced Trx is to bind and inhibit ASK1, whereas oxidized Trx dissociates from ASK1 [43,51,52]. We therefore examined Trx1/ASK1 association as another cellular indicator of the functional redox state of Trx1. In untreated cells in which >95% of Trx1 is reduced (Fig. 4), ASK1 is bound to Trx1 (Fig. 8)."
"Another function of reduced Trx is to bind and inhibit ASK1, whereas oxidized Trx dissociates from ASK1 [43,51,52]. We therefore examined Trx1/ASK1 association as another cellular indicator of the functional redox state of Trx1. In untreated cells in which >95% of Trx1 is reduced (Fig. 4), ASK1 is bound to Trx1 (Fig. 8)."
"Apoptosis signal-regulating kinase 1 (ASK1), a member of the mitogen-activated protein kinase kinase kinase family, plays pivotal roles in reactive oxygen species (ROS)-induced cellular responses. In resting cells, endogenous ASK1 constitutively forms a homo-oligomerized but still inactive high-molecular-mass complex including thioredoxin (Trx), which we designated the ASK1 signalosome. Upon ROS stimulation, the ASK1 signalosome unbinds from Trx and forms a fully activated higher-molecular-mass complex"