IndraLab

"KCNB1 forms macromolecular complexes with integrins in the mouse brain xref ."

"Kv2.1 also forms complexes with integrins that are essential for the migration of glutamatergic neurones during prenatal brain development and regulates migration through nonionic functions."

"KCNB1 forms complexes with integrins named IKCs, that regulate the migration of excitatory glutamatergic neurons during corticogenesis. xref Accordingly, Knock In mice harboring a missense ( Kcnb1 R312H ) mutation found in children with developmental and epileptic encephalopathy (DEE), an umbrella term to describe pathologies in which severe developmental delay coexists with epilepsy, xref , xref exhibit neocortical malformations and defective pyramidal neuron morphology that are due to impairment of the signaling machinery of the IKC. xref Since integrins are broadly expressed in the brain, we asked whether they were also present in KCNB1‐LepR complexes."

"To determine whether KCNB1 and integrins form complexes in the human brain, proteins were immunoprecipitated (IP) with an antibody that detects integrin alpha chain V (integrin-alpha5) and immunoblotted (IB) with an antibody that detects KCNB1 or vice versa."

"Further, KCNB1 forms macromolecular complexes with integrins that will be discussed in some detail in this review (Yu et al., 2017)."

"Here we show that the apoptotic signal originates from integrins, which form macromolecular complexes with KCNB1 channels."

"Here, we investigate the function of neocortical IKCs formed by voltage-gated potassium (Kv) channels Kcnb1 and α5β5 integrin dimers in wild-type (WT) and homozygous knock-in (KI) Kcnb1 R312H(+/+) mouse model of DEE."

"Like other Kv channels, Kcnb1 forms stable complexes with integrins, named Integrin_K + Channel_Complexes or IKCs xref ."

"Moreover, if Kcnb1 interacts with the integrins of the IKC, blocking the channel with GxTx should affect the fibronectin response."

"This may suggest that the voltage-sensor has a role in mediating Kcnb1-integrin interactions and consequently, that the histidine replacement uncouples these interactions in IKCs formed with R312H channels."

"We next determined whether KCNB1 formed a complex with integrins in the mouse brain."

"R312H KCNB1 subunits formed complexes with α5β5 integrins, but in homozygotes the signaling of those macromolecules was grossly compromised."

"In vivo, tetramers that assemble only by α subunits are called homotetrameric Kv2.1 channels, whereas tetramers assembled by both α subunits and other families of channel-forming subunits are referred to as heterotetrameric Kv2.1 channels.[ xref ]Homotetrameric Kv2.1 channels contribute to a significant fraction of delayed rectifier potassium currents in various neuronal subtypes, including pyramidal neurones in the hippocampus and cortex, are more complex, and depend on the subunit composition of the channel.[ xref , xref ]In vitro and in vivo experiments have verified that the pathogenic mechanism of KCNB1 -related diseases involves channel hypofunction, altered voltage dependence, reduced protein expression, or cell surface trafficking due to KCNB1 gene variants.[ xref , xref ]Kv2.1 also forms complexes with integrins that are essential for the migration of glutamatergic neurones during prenatal brain development and regulates migration through nonionic functions.[ xref ]"

"One K + channel known to form IKCs is KCNB1, which assembles with α5β5 integrin dimers in the neocortex ( xref ; Yu et al., 2017; Bortolami et al., 2022)."