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RPS6KB1 phosphorylates IRS1 on S307. 45 / 45
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reach
"We observed that S6K1-dependent phosphorylation of IRS1 on S307 and S1101 was diminished in SH-SY5Y cells treated with miR-200b and miR-200c.The oAβ leads to additional generation of oAβ resulted from abnormal autophagosome accumulation via inhibition of mTOR pathway [17]."

rlimsp
"Our findings demonstrate that it is unlikely that S6K1 is the protein kinase that phosphorylates IRS1 at Ser307 in response to insulin stimulation of human adipocytes."

reach
"The very different dynamics for phosphorylation of IRS1 at Ser307, on one hand, and of S6K1 at threonine 389 and S6 at serine 235/236, on the other, suggest that IRS1 is not phosphorylated at Ser307 by S6K1."

rlimsp
"This led to phosphorylation of IRS-1 by S6K1 at multiple serine residues including Ser-270, Ser-307, Ser-636, and Ser-1101 in human IRS-1 (Ser-265, Ser-302, Ser-632, and Ser-1097, in rodent IRS-1). Direct phosphorylation of these sites by S6K1 was observed in an in vitro kinase assay using purified IRS-1 and S6K1."

reach
"In in vitro kinase assays, S6K1 directly phosphorylates S302 of mouse IRS-1, a site matching its preferred substrate recognition motif."

reach
"Most likely, mTORC1 itself, rather than S6K1, phosphorylates IRS1 on Ser 307 to effect the inhibition of PI3K-Akt signaling, subsequently suppressing myogenic differentiation."

reach
"Activation of the mTOR pathway functions as a feedback regulator of insulin action by increasing phosphorylation of IRS1 at Ser307 [pIRS (S307)] and Ser636/639 [pIRS (S636/639)] by S6K1 and mTOR, respectively."

sparser
"Consistent with these observations it was found that S6K1 and S6K2 phosphorylate IRS-1 in vitro at S302, a site adjacent to its phosphotyrosine-binding (PTB) domain."

sparser
"We observed that S6K1-dependent phosphorylation of IRS1 on S307 and S1101 was diminished in SH-SY5Y cells treated with miR-200b and miR-200c."

rlimsp
"This led to phosphorylation of IRS-1 by S6K1 at multiple serine residues including Ser-270, Ser-307, Ser-636, and Ser-1101 in human IRS-1 (Ser-265, Ser-302, Ser-632, and Ser-1097, in rodent IRS-1)."

reach
"First, it was found that S6K1 and S6K2 phosphorylate IRS-1 on serine 302, a site adjacent to its PTB domain."

rlimsp
"A point mutant of IRS-1 (S270A) impaired association of IRS-1 with S6K1 resulting in diminished phosphorylation of IRS-1 at three other S6K1 phosphorylation sites (Ser-307, Ser-636, and Ser-1101)."

rlimsp
"We report that, by several criteria, S6K1 does not phosphorylate IRS1 at serine 307 in response to insulin in intact human primary adipocytes: (i) The time-courses for phosphorylation of S6K1 and its phosphorylation of S6 are not compatible with the phosphorylation of IRS1 at serine 307; (ii) A dominant-negative construct of S6K1 inhibits the phosphorylation of S6, without effect on the phosphorylation of IRS1 at serine 307; (iii) The specific inhibitor of S6K1 PF-4708671 inhibits the phosphorylation of S6, without effect on phosphorylation of IRS1 at serine 307."

"Nevertheless, s6k1-deficient mice remain sensitive to insulin owing to the apparent loss of a negative feedback loop from s6k1 to insulin receptor substrate 1 (irs1), which blunts s307 and s636/s639 phosphorylation; thus under conditions of nutrient satiation s6k1 negatively regulatesinsulin."

reach
"In addition, when mTORC1 is activated, S6K1 could directly phosphorylate Insulin receptor substrate 1 (IRS1; S307 and S636 and S639) and promote its degradation, which subsequently blunts phosphoinositide 3-kinase (PI3K)-AKT activation and its downstream effects such as glucose uptake and glycogen accumulation."

sparser
"The non-compatible time-courses, the selective effect of S6K1-DN, and the selective effect of the S6K1 inhibitor, each analyzes a very different aspect of signaling by S6K1 and each indicates non-compatibility with S6K1 phosphorylating IRS1 at Ser307."

reach
"IRS-1 S302 and S632 (S307 and S636, respectively, in humans) are directly phosphorylated by S6K1 and mTORC1, respectively (Copps and White, 2012)."

reach
"S6K1 phosphorylates insulin receptor substrate 1 (IRS-1) at Ser1101 and Ser302."

reach
"These include mammalian target of rapamycin (mTOR)-mediated phosphorylation of IRS1 serine 636 (Ser636) and serine 639 (Ser639), ribosomal S6 kinase 1 (S6K1)-mediated phosphorylation of IRS1 serine 307 [Ser307 (mouse serine 302) (Ser302)] and serine 1101, IkappaB kinase beta (IKKbeta) - and c-Jun N-terminal kinase (JNK)-mediated phosphorylation of IRS1 serine 312 [Ser312 (mouse Ser307)], and protein kinase zeta mediated phosphorylation of IRS1 serine 323 (mouse serine 318)."

reach
"The findings of the present study of diet-and obesity induced insulin resistance in murine models suggest that suppression of TSC1 by IKKbeta activates mTOR and S6K1, which in turn phosphorylate IRS1 at Ser636/639 and Ser307, thereby inhibiting IRS1 function)."

sparser
"Phosphorylation at Ser-302 of IRS-1 by S6K1 results in disruption of its interaction with insulin receptor and inhibits PI3K-mediated Akt activation ( xref ) ( xref )."

sparser
"We report that, by several criteria, S6K1 does not phosphorylate IRS1 at serine 307 in response to insulin in intact human primary adipocytes: (i) The time-courses for phosphorylation of S6K1 and its phosphorylation of S6 are not compatible with the phosphorylation of IRS1 at serine 307; (ii) A dominant-negative construct of S6K1 inhibits the phosphorylation of S6, without effect on the phosphorylation of IRS1 at serine 307; (iii) The specific inhibitor of S6K1 PF-4708671 inhibits the phosphorylation of S6, without effect on phosphorylation of IRS1 at serine 307."

rlimsp
"P70S6K phosphorylates IRS-1 in serine 307 which leads to the uncoupling between IRS-1 and p85 (the regulatory subunit of PI3K) and therefore causing the lack of response of HepG2 to IGF-1."

sparser
"We did not observe elevated levels of components of mTOR/S6K pathway in case of single Pten or Lkb1 deletion in the bladder, however we show that Lkb1 deletion led to increased Ser302 phosphorylation of IRS-1 (equivalent to human Ser307 residue) by p70S6K ( xref )."

reach
"The mTORC1-downstream p70 ribosomal protein S6 kinase (S6K1), which is activated by insulin, can phosphorylate IRS1 at serine 307 in vitro and is considered the physiological protein kinase."

reach
"P70S6K phosphorylates IRS-1 in serine 307 which leads to the uncoupling between IRS-1 and p85 (the regulatory subunit of PI3K) and therefore causing the lack of response of HepG2 to IGF-1."

No evidence text available

sparser
"In in vitro kinase assays, S6K1 directly phosphorylates S302 of mouse IRS-1 ( xref ), a site matching its preferred substrate recognition motif ( xref )."

sparser
"The very different dynamics for phosphorylation of IRS1 at Ser307, on one hand, and of S6K1 at threonine 389 and S6 at serine 235/236, on the other, suggest that IRS1 is not phosphorylated at Ser307 by S6K1."

reach
"Consistent with these observations it was found that S6K1 and S6K2 phosphorylate IRS-1 in vitro at S302, a site adjacent to its phosphotyrosine binding (PTB) domain."

rlimsp
"The non-compatible time-courses, the selective effect of S6K1-DN, and the selective effect of the S6K1 inhibitor, each analyzes a very different aspect of signaling by S6K1 and each indicates non-compatibility with S6K1 phosphorylating IRS1 at Ser307."

reach
"When mTORC1 is chronically activated following excessive glucose or branched chain amino acid consumption, the sustained simulation of downstream S6K1 increases IRS1 Ser307 phosphorylation."

No evidence text available

reach
"Western blot images showed that S6K1-dependent phosphorylation of IRS-1 on S307 and S1101, but not IRS-1 itself, were decreased in miR-200b- and miR-200c-transfected SH-SY5Y cells (Fig 5)."

sparser
"The insulin- and nutrient-stimulated kinase S6k1 phosphorylates Irs1 at Ser302, as well as other putatively inhibitory sites ( xref )."

rlimsp
"S6K1 was found to phosphorylate a large fragment of IRS1 at Ser307 in vitro, and insulin-induced phosphorylation was blocked by knock-down of S6K1 in TSC2−/− mouse embryo fibroblasts [36]."

sparser
"P70S6K phosphorylates IRS-1 in serine 307 which leads to the uncoupling between IRS-1 and p85 (the regulatory subunit of PI3K) and therefore causing the lack of response of HepG2 to IGF-1."

sparser
"Western blot images showed that S6K1-dependent phosphorylation of IRS-1 on S307 and S1101, but not IRS-1 itself, were decreased in miR-200b- and miR-200c-transfected SH-SY5Y cells ( xref )."

sparser
"S6K1 phosphorylates Ser-307 of insulin receptor substrate-1 (IRS-1) and thereby activates an important feedback mechanism, which downregulates insulin signalling by inducing insulin resistance [ xref – xref ]."

sparser
"IRS-1 S302 and S632 (S307 and S636, respectively, in humans) are directly phosphorylated by S6K1 and mTORC1, respectively ( Copps and White, 2012 )."

reach
"The non compatible time-courses, the selective effect of S6K1-DN, and the selective effect of the S6K1 inhibitor, each analyzes a very different aspect of signaling by S6K1 and each indicates non compatibility with S6K1 phosphorylating IRS1 at Ser307."

reach
"The insulin- and nutrient stimulated kinase S6k1 phosphorylates Irs1 at Ser302, as well as other putatively inhibitory sites."

sparser
"CCR5 deficiency desensitizes insulin signaling by increasing p70 S6 kinase-mediated serine 302 phosphorylation of IRS-1."

reach
"S6K1 phosphorylates Ser 307 of insulin receptor substrate-1 (IRS-1) and thereby activates an important feedback mechanism, which downregulates insulin signalling by inducing insulin resistance [XREF_BIBR - XREF_BIBR]."

reach
"When mTORC1 is activated, S6K1 could directly phosphorylate IRS1 (S307 and S636/S639) and promote its degradation, which subsequently blunts PI3K-AKT activation and its downstream effects such as glucose uptake, glycogen accumulation, etc. [2,3]."