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SCN5A binds VCL. 21 / 21
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"Interestingly, we found the interaction between VCL and SCN5A were not affected by the M94I mutation of VCL (XREF_FIG, D)."
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"According to our findings that VCL directly interacts with SCN5A invivo and invitro and that VCL and SCN5A were colocalized at the ID in the human heart, this functional effect may result from the direct association between VCL and SCN5A.17 However, the fact that VCL-D841H did not affect the interaction between VCL and SCN5A suggested that the molecular mechanism for this functional interaction remains to be addressed."
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"Vinculin Physically and Functionally Interacts With SCN5A."
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"In this study, we initially identified that VCL interacts directly with SCN5A in both mouse heart and HEK293 cells transiently expressing VCL and SCN5A."
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"Co-immunoprecipitation also showed that VCL-M94I did n't affect the physical interaction between VCL and SCN5A."
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"VCL was also confirmed to directly interact with SCN5A, and VCL-D841H did not disrupt the association of VCL and SCN5A. A VCL common variant was genetically and biophysically associated with Chinese SUNDS."
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"These results suggest that VCL-M94I doesn’t disrupt the physical association of VCL and SCN5A."
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"VCL directly interacts with SCN5A in vivo and in vitro ."
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"To explore the relationship between VCL and SCN5A, we used co-immunoprecipitation experiments to examine if VCL physically interacts with SCN5A. In mouse heart tissues, immunoblot analysis of immunoprecipitated VCL detected the presence of SCN5A, and we also found VCL present following a reciprocal immunoprecipitate using the antibody against SCN5A; due to the negative expression of SCN5A, mouse liver tissue was used as a control ( xref )."
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"The interaction between VCL and SCN5A were also observed in HEK293 cells overexpressing VCL and SCN5A (XREF_FIG), further confirming their direct interaction."
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"As previously reported, xref we identified that VCL directly interacts with SCN5A in vivo and in vitro using coimmunoprecipitations (Figure  xref A and xref B)."
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"VCL directly interacts with SCN5A in vivo and in vitro."
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"Interestingly, we found the interaction between VCL and SCN5A were not affected by the M94I mutation of VCL ( xref ,D)."
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"Through biophysical investigation, we have now linked a VCL variant M94I to the loss-of-function of cardiac sodium channel in both HEK293 cells and iPSC-CMs, showing a previously unknown functional interaction between VCL and SCN5A."
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"To explore the relationship between VCL and SCN5A, we used co-immunoprecipitation experiments to examine if VCL physically interacts with SCN5A."
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"Co-immunoprecipitation also showed that VCL-M94I didn’t affect the physical interaction between VCL and SCN5A. These results suggest the observed functional effect of mutant VCL-M94I on SCN5A does not involve expression and distribution of VCL or SCN5A. The precise mechanism for decreased I Na observed ( xref and xref ) is unclear."
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"More detailed biophysical mechanisms for the alteration of sodium channel kinetics and the functional interaction of VCL with SCN5A remain to be identified."
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"In this study, we initially identified that VCL interacts directly with SCN5A in both mouse heart and HEK293 cells transiently expressing VCL and SCN5A. Based on these studies, we speculated that dysfunction of VCL may affect sodium channel function."
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"Through biophysical investigation, we have now linked a VCL variant M94I to the loss-of-function of cardiac sodium channel in both HEK293 cells and iPSC-CMs, showing a previously unknown functional interaction between VCL and SCN5A. In the myocardial section from the VCL-M94I carrier, we confirmed the co-localization of VCL and SCN5A at ID, but didn’t find significant difference in distribution of both VCL and SCN5A compared to control."
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"The interaction between VCL and SCN5A were also observed in HEK293 cells overexpressing VCL and SCN5A ( xref ), further confirming their direct interaction."
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"Furthermore, VCL-D841H does not affect this physical association between VCL and SCN5A."
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