IndraLab

Statements

PSMD8 binds HAUS7 and UCHL5. 5 / 5
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"Here, we report the identification of two proteins, S14 and UCH37 interacting protein 1 (UIP1), which interact with UCH37."
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"The Y187 yeast strain harbouring S14/pGAD-GH or UIP1/pGAD-GH was mated with PJ69-2A strain transformed with pU1, pU2 or pU3 respectively to test the interaction between S14 or UIP1 with UCH37, UCH37ΔC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We carried out in vitro binding assays to confirm that S14 or UIP1 could individually bind UCH37 in vitro under conditions other than that of the yeast two-hybrid system, As shown in Fig. 3A,B [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"These results indicate that S14 and UIP1 could bind UCH37 specifically in vitro in a C-terminal extension dependent manner."
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"In the present study we found that UCH37 interact with UIP1 and S14 in a C-terminal extension dependent manner, and UIP1 was found to interact with the C-terminal extension of UCH37 alone by yeast two[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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